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DNA amplification is one of the most valuable tools for the clinical diagnosis of nucleic acid-related diseases, but current techniques for DNA amplification are based on intermolecular polymerization reactions, resulting in the risk of errors in the intermolecular reaction pattern. In this article, we introduce the concept of intramolecular polymerization and isomerization cyclic amplification (PICA), which extends a short DNA strand to a long strand containing periodic repeats of a sequence through cyclic alternating polymerization and isomerization. To the best of our knowledge, this is the first time that a real ssDNA self-extension method without any additional auxiliary oligonucleotides has been reported. By interfacing PICA with external molecular elements, it can be programmed to respond to different targets. Herein, we designed two distinct types of amplified nucleic acid detection platforms that can be implemented with PICA, including cyclic reverse transcription (CRT) and cyclic replication (CR). We experimentally demonstrate the mechanisms of CRT-PICA and CR-PICA using mammalian miRNA and virus DNA. The results showed that this proposed detection platform has excellent sensitivity, selectivity, and reliability. The detection level could reach the aM level, that is, several copies of target molecules can be detected if a small volume is taken into account., DNA amplification is one of the most valuable tools for the clinical diagnosis of nucleic acid-related diseases, but current techniques for DNA amplification are based on intermolecular polymerization reactions, resulting in the risk of errors in the intermolecular reaction pattern.

Food additives are widely used in our daily life, and the side-effects caused by them have gained extensive attention around the world. Notably, constituent-oriented metabolites, in some sense, always contribute to pharmacological changes, inducing toxicity, therapeutic effects, etc. Characterization of the metabolites and their potential functions is of great importance to the practical applications. In this work, an integrated strategy by combining metabolite profiling and network pharmacology was applied to characterize the metabolic features and reveal pharmacological changes of neohesperidin dihydrochalcone (NHDC) in vivo to demonstrate its pharmacological mechanism and potential functions. As a result, a total of 19 metabolites (3 in plasma, 19 in urine, 8 in feces, 3 in heart, 5 in liver, 0 in spleen, 1 in lung, 2 in kidneys and 2 in brain) were screened and 18 of them were characterized for the first time. Phase I metabolic reactions of hydrolysis and phase II reactions of glucuronidation, sulfation, glutamylation, N-butyryl glycylation and lactylation were the main metabolic reactions of NHDC in vivo. Moreover, the results analyzed by network pharmacology revealed that, in addition to common pathways (steroid hormone biosynthesis) of NHDC, metabolites' targets were involved in pathways in cancer, ovarian steroidogenesis, proteoglycans in cancer, PI3K-Akt signaling pathway and progesterone-mediated oocyte maturation, indicating that these functional changes might result in potential novel functions or other side-effects, such as a disorder of steroid hormones. Our work provided the metabolic features and functional modifications of NHDC in vivo for the first time, and meaningful information for further pharmacological validations or potential functions is supplied.

The dynamic cone penetration test (DCPT) is a common method for assessing the physical and mechanical properties of cohesionless soils. However, current formulas might not be suitable for carbonate...

Antisense oligodeoxynucleotide (ASODN) can directly interfere a series of biological events of the target RNA derived from tumor cells through Watson-Crick base pairing, in turn, plays antitumor therapeutic roles. In the study, a novel HIF-1α ASODN-loaded nanocomposite was formulated to efficiently deliver gene to the target RNA. The physicochemical properties of nanocomposite were characterized using TEM, FTIR, DLS and zeta potentials. The mean diameter of resulting GEL-DGL-FA-ASODN-DCA nanocomposite was about 170–192 nm, and according to the agarose gel retardation assay, the loading amount of ASODN accounted for 166.7 mg/g. The results of cellular uptake showed that the nanocomposite could specifically target to HepG2 and Hela cells. The cytotoxicity assay demonstrated that the toxicity of vectors was greatly reduced by using DCA to reversibly block the cationic DGL. The subcellular distribution images clearly displayed the lysosomal escape ability of the DCA-modified nanocomposite. In vitro exploration of molecular mechanism indicated that the nanocomposite could inhibit mRNA expression and HIF-1α protein translation at different levels. In vivo optical images and quantitative assay testified that the formulation accumulated preferentially in the tumor tissue. In vivo antitumor efficacy research confirmed that this nanocomposite had significant antitumor activity and the tumor inhibitory rate was 77.99%. These results manifested that the GEL-DGL-FA-ASODN-DCA nanocomposite was promising in gene therapeutics for antitumor by interacting directly with target RNA., Graphical abstract A novel HIF-1α antisense oligodeoxynucleotide-loaded nanocomposite was formulated to deliver efficiently gene to the target RNA. The nanocomposite could inhibit mRNA expression and HIF-1α protein translation in vitro. In vivo the nanocomposite had significant antitumor activity. Image, graphical abstract

The fission reactions of 238U induced by D-T neutrons were investigated by neutron activation technique and low-background HPGe-γ spectrometer. The half-life was used to identify the possible daughter nuclide. The fission cross-sections of 238U(n,f)89Rb reaction induced at the neutron energy around 14 MeV, i.e., 14.1 ± 0.3, 14.5 ± 0.3 and 14.7 ± 0.3 MeV, were measured precisely. The neutron flux was monitored by accompanying α-particle of the T(d,n)4He reaction and the neutron energies were determined by the cross-section ratio of 90Zr(n,2n)89Zr to 93Nb(n,2n)92mNb reaction. The fission cross-sections of the 238U(n,f)89Rb reaction are 33.2 ± 2.1 mb, 32.2 ± 2.0 mb, and 32.6 ± 2.1 mb, corresponding to the above neutron energy points.

Short gamma-ray bursts (GRB) with extended emission (EE) that are composed of an initial short hard spike followed by a long-lasting EE are thought to comprise a sucategory of short GRBs. The narrow energy band available during the Swift era, combined with a lack of spectral information, prevented the discovery of the intrinsic properties of these events. In this paper, we perform a systematic search of short GRBs with EE using all available Fermi/GBM data. The search identified 26 GBM-detected short GRBs with EE that are similar to GRB 060614 observed by Swift/BAT. We focus on investigating the spectral and temporal properties of both the hard spike and the EE component of all 26 GRBs, and explore differences and possible correlations between them. We find that while the peak energy (Ep) of the hard spikes is slightly harder than that of the EE, their fluences are comparable. The harder Ep seems to correspond to a larger fluence and peak flux, with a large scatter for both the hard spike and the EE component. Moreover, the Ep of both the hard spike and the EE are compared with other short GRBs. Finally, we also compare the properties of GRB 170817A with those of short GRBs with EE and find no significant statistical differences between them. We find that GRB 170817A has the lowest Ep, probably because it is off-axis.

In this study, the gamma-ray spectrum of single elemental capture spectrum log was simulated. By numerical simulation we obtain a single-element neutron capture gamma spectrum. The neutron and photon transportable processes were simulated using the Monte Carlo N-Particle Transport Code System (MCNP), where an Am-Be neutron source generated the neutrons and thermal neutron capture reactions with the stratigraphic elements. The characteristic gamma rays and the standard gamma spectra were recorded, from analyzing of the characteristic spectra analysis we obtain the ten elements in the stratum, such as Si, Ca, Fe, S, Ti, Al, K, Na, Cl, and Ba. Comparing with single elemental capture gamma spectrum of Schlumberger, the simulated characteristic peak and the spectral change results are in good agreement with Schlumberger. The characteristic peak positions observed also consistent with the data obtained from the National Nuclear Data Center of the International Atomic Energy Agency. The neutron gamma spectrum results calculated using this simple method have practical applications. They also serve as an reference for data processing using other types of element logging tools.

A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China. Growth was observed at 10–30 °C (optimum, 25 °C), in 0–7.0 % NaCl (0 %) and at pH 6.0–11.0 (pH 8.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus , and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon. The DNA G+C content was 44.2 mol%. The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp. nov. is proposed. The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).

Attached microalgae cultivation for biomass/bioenergy production has been proposed these decades to avoid the energy-intensive harvest process. However, the external light could only propagate

The sustainable operation of horizontal subsurface flow constructed wetlands (HSSF CWs) is challenging due to the accumulation of organic and inorganic solids in substrate interspaces. In this study, wetland microcosms with (EC-CWs) and without (C-CWs) external carbon source were monitored for 120 days to investigate the impact of extracellular polymeric substances (EPS) on inorganic particle settlement and nutrient removal of HSSF CWs. EPS flocculation aggravated the solids accumulation in the inlet zone of the EC-CWs, and ion bridging through EPS and sweep flocculation were the main flocculation mechanisms. EPS increased the hydraulic resistance of the accumulated solids, which almost halved the hydraulic retention time (HRT) of the EC-CWs. Compared with the C-CWs, the media storage of total phosphorus (TP) in the EC-CWs was improved by 70.18 ± 2.74%, which was mainly due to the increased adsorption sites introduced by EPS. RDA analysis showed that solids accumulation in the HSSF CWs was positively correlated with the TP removal, but the process had a minor influence on organic matter and nitrogen removal. Combined analyses revealed that the accumulation of EPS and inorganic particle not only caused HRT reduction but also introduced abundant adsorption sites. The present results would promote the understanding on role of EPS in sustainable operation of HSSF CWs, and further maintain the stable nutrient removal performance.

The application of subsurface-flow constructed wetlands (SSFCWs) has been restricted by the low nitrogen and phosphorus removal efficiency for several decades. Ferric-Carbon Micro-Electrolysis (Fe/C-M/E) system has gained increasing attention due to its high redox capacity and the function of adsorption and flocculation. Therefore, to apply Fe/C-M/E process in SSFCWs is a promising approach to enhance the removal efficiency of nitrogen and phosphorus of domestic sewage. In this study, a micro-electrolysis-intensified subsurface-flow constructed wetlands (ME-SSFCWs) including iron scraps and biochar were operated to investigate the effect of micro-electrolysis-substrate on nitrogen and phosphorus removal performance together with the related kinetics and possible pathways. The results showed that the removal efficiency of NO3−-N, total nitrogen (TN) and phosphorus by ME-SSFCWs were 99.54 ± 0.80%, 81.45 ± 1.27% and 93.63 ± 5.30%, respectively, for a 3-day hydraulic retention time (HRT), which were much higher than that of the ordinary SSFCWs (NO3−-N: 37%; TN: 38%; TP: 39%) and biochar-added SSFCWs (NO3−-N: 57%; TN: 54%; TP: 21%). These results indicated that micro-electrolysis could supply electrons for the denitrification. Besides, the improvement of phosphorus removal efficiency was due to the galvanic corrosion of numerous micro-scale sacrificial anodes which caused precipitation, adsorption and flocculation of phosphorus. Microbial analysis via real-time quantitative PCR Detecting System (qPCR) revealed that associated microbial abundance could dramatically increase due to the addition of Fe/C-M/E material substrate.

Twelve-coordinated sulfate hydrogen bonding interactions in water-containing Fe(II) system, [Fe0.5(H2O)3]2SO4·H2O (1), are reported herein. The X-ray single-crystal structural characterization sugg...

Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR. It takes advantage of the novel designed variable primer, which is initially designed to be linear, extending to form a hairpin structure and replacing the target miRNA for cyclic RT. Then the RT product is quantified by conventional EvaGreen based qPCR. The results show that this method has a dynamic range of 8 logs and the sensitivity is sufficient to directly detect down to 4 target miRNA molecules with a total analysis time of less than 2 hours. It is capable of discriminating between similar miRNAs, leading to an accurate representation of the mature miRNA content in a sample. The RT step can be multiplexed and the 8 miRNA profiles measured in 7 mouse tissues by this method show an excellent correlation with the commercial standard TaqMan RT-qPCR assays (r2 = 0.9881).

Microorganisms in sediments are an important part of the aquatic ecosystem, and their functional activities are sensitive to external environmental pressure. Shallow lakes are characterized by frequent sediment resuspension events, leading to large amounts of nutrients being released. However, information about the potential impacts of sediment resuspension events on the functional activities of microbial communities is limited. In this study, the responses of microbial carbon metabolism in sediments under different wind&ndash, wave disturbance were analyzed by BIOLOG ECO microplates. The results showed that under four disturbance conditions (wind speeds of 0, 1.60, 3.62, and 14.10 m/s), the total carbon metabolism function of the sediment microbial community (represented as average well-color development, AWCD) remained unchanged (p &gt, 0.05), and the final total AWCD value stabilized at about 1.70. However, compared with the control group, some specific carbon sources (e.g., amines and carboxylic acids) showed significant changes (p &lt, 0.05). We found that short-term (8 h) resuspension events did not affect the total carbon metabolism of sediment microbial communities, while it affected the microbial utilization ability of some specific types of carbon sources. For example, we found that the microbial utilization capacity of polymers in the 14.10 m/s group was the best. This study provides a new insight into the carbon cycle process of shallow lake sediments that resuspension events will affect the carbon cycle process of sediments.

The successful operation of dedicated detectors has brought us valuable information for understanding the central engine and the progenitor of gamma-ray bursts (GRBs). For instance, the giant X-ray and optical bumps found in some long-duration GRBs (e.g. GRBs 121027A and 111209A) imply that some extended central engine activities, such as the late X-ray flares, are likely due to the fall-back of progenitor envelope materials. Here we systemically search for long GRBs that consist of a giant X-ray or optical bump from the Swift GRB sample, and eventually we find 19 new possible candidates. The fall-back accretion model could well interpret the X-ray and optical bump for all candidates within a reasonable parameter space. Six candidates showing simultaneous bump signatures in both X-ray and optical observations, which could be well fitted at the same time when scaling down the X-ray flux into optical by one order of magnitude, are consistent with the standard $F_{\nu}\propto\nu^{1/3}$ synchrotron spectrum. The typical fall-back radius is distributed around $10^{10}\rm-10^{12}$ cm, which is consistent with the typical radius of a Wolf-Rayet star. The peak fall-back accretion rate is in the range of $\sim 10^{-11}-10^{-4}M_{\odot} \ \text{s} ^{-1}$ at time $\sim10^{2}- 10^{5}~\rm s$, which is relatively easy to fulfill as long as the progenitor's metallicity is not too high. Combined with the sample we found, future studies of the mass supply rate for the progenitors with different mass, metallicity, and angular momentum distribution would help us to better constrain the progenitor properties of long GRBs., Comment: accepted for publication by ApJ

Lonicerae japonicae flos.(LJF) was widely used as a drug to treat upper respiratory tract infection or a tea to clear heat in Asian countries for thousands of years. Despite of its curative effects confirmed by modern pharmacological methods, its functional materials and mechanism against influenza were still unclear and needed further investigation. In this study, an integrated strategy based on in vivo substances profiling and network pharmacology was proposed and applied to screen out the potential anti-influenza substances and mechanism of LJF. An UHPLC/Q-TOF MS method was utilized to profile the chemical components in LJF and their metabolites in rats. The targets of absorbed prototypes were predicted by Swiss Target Prediction, and they were further analyzed by String and Kyoto Encyclopedia of Genes and Genomes (KEGG). As a result, a total of 126 chemical components mainly featuring three chemical structure types were characterized, including 70 iridoid glycosides, 17 caffeoylquinic acids, 24 flavonoids, and 15 other types compounds. Among them, ten N-contained iridoid glycosides were characterized as potential novel compounds. Moreover, 141 xenobiotics (74 prototypes and 67 metabolites) were clearly screened out in rat plasma and urine after ingestion of LJF. Phase II reactions (sulfation, glucuronidation, methylation) and phase I reactions (dehydroxylation, hydrogenation, hydrolysis, N-heterocyclization) were the main metabolic reactions of LJF in rats. Further, a total of 338 targets were predicted and TNF, PTGS2 and EGFR were the three main targets involved in the pathology of influenza. In addition to normal NF-κB pathway, T cell signal pathway and mTOR signal pathway were the other patterns for LJF to achieve its anti-flu effects. Our work provided the meaningful data for further pharmacological validation of LJF against influenza, and a new strategy was also proposed for minimizing the process to reveal the mechanism and functional basis of TCMs.

The disposal of reverse osmosis (RO) concentrate (ROC) is a critical challenge impeding the application of RO-based wastewater reclamation. Herein, we proposed an enhanced biotreatment approach for the simultaneous removal of nitrogen, phosphorous, hardness, and methylisothiazolinone (MIT) from ROC by suspended-solid phase cultivation of Scenedesmus sp. LX1. Repeated carrier addition, guided by the developed optimal carrier addition model, efficiently enhanced algal growth and contaminant removal through dynamically controlling the suspended algal density by cell attachment. The maximum algal growth rate (212.2 mg/(L∙d)) increased by 41% compared with the control, and the time needed for reaching the maximum algal biomass (906.7 mg/L) was shortened by 1 d, attributing to the mitigation of density restriction. 91.8% of nitrogen (30.2 mg/L) was removed with 5.5 mg/(L∙d) accelerating removal rate, and phosphate (3.7 mg/L) was completely removed within 1 d. Hardness precursors calcium and inorganic carbon were also removed in large amounts, 268.4 and 128.2 mg/L, respectively. Moreover, suspended-solid phase cultivation significantly mitigated the growth inhibition caused by MIT toxicity, enabled the algae to completely biodegrade MIT of extremely high concentrations (4.7 mg/L and 11.4 mg/L) in a short time. Our results demonstrate the feasibility of suspended-solid phase algal cultivation for simultaneously and effectively removing multiple main contaminants from ROC.

Traditional Chinese medicine was widely used in China since its definite effects and therapy. The components of TCM were absorbed into the circle system as the format of prototypes or metabolites, which contributed to the therapy or side effects. Declaring the functional changes in this process was of great importance to the clinical applications. In this work, an integrated strategy based on metabolites’ profiling and network pharmacology was proposed for exploring the pharmacological changes of compounds in vivo. Arctiin, the main component in Fructus Arctii with various kinds of bioactivities, was used as an example. An ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry and metabolynx™ software was applied to characterize the metabolites of arctiin in rats at a dosage of 100 mg/kg; network pharmacology was applied to characterize the functional changes. As a result, fifty-three metabolites (32 in plasma, 40 in urine, 19 in bile, 20 in feces, 1 in brain, 12 in liver and 4 in lungs) were screened out and characterized, and 3 of them were unambitiously identified by comparison with standard substances. Among them, 38 metabolites were reported for the first time. It was found the major metabolic pathways of arctiin in rats were demethylation, lactone-opening and phase II conjugations with sulfate and glucuronide. It also confirmed that M14, M15, M18, M23, M22, M43 and M45 were the major circulating forms of arctiin in rats following oral administration. In addition to the above metabolic reactions, phase I reactions of hydrolysis, demethylation, dehydroxylation were also observed, and dehydrogenation were first revealed metabolic patterns of arctiin in rats. Meanwhile, in addition to the main targets of arctiin (MTOR, EGFR and MAPK14), its metabolites targeted additional 392 targets with additional functions of anti-hepatitis B or viral carcinogenesis (SRC, CAPS3, PIK3CA, CDK4, ESR1, MMP9 and ERBB2). The above results provided very important information for understanding the metabolism and functional changes of arctiin in vivo, and supporting data for further pharmacological evaluation. Our work also provided a newsight for elucidation of functional changes of TCMs in vivo.

Non-suspended microalgae cultivation technology coupled with wastewater purification has received more scientific attention in recent decades. Since the non-suspended microalgae cultivation is quite different from the suspended ones, the following issues are compared in this study such as advantages and disadvantages, pollutant removal mechanisms and regulations, influential factors, and microalgae biomass accumulation. The analysis aims to support the further application of this technology. The median removal rates of COD, TN, TP, NH4+-N and NO3−-N were 91.6%, 78.2%, 87.5%, 93.2% and 81.7%, respectively, by non-suspended microalgae under the TN & TP load rates up to 150 mg·L−1·d−1. The main pathway for TN & TP removal is microalgae cell absorbance. Light intensity, pollutant composition and microalgae metabolic types are the major factors that influence pollutant removal and the lipid content of microalgae. Meanwhile the mechanism concerning how macro-outer conditions influence the micro-environment and further growth of non-suspended microalgae requires more investigation.

The observed internal plateau of X-ray emission in some short GRBs suggests the formation of a remnant supra-massive magnetar following a double neutron star (NS) merger. In this paper, we assume that the rotational energy is lost mainly via gravitational wave (GW) radiation instead of magnetic dipole (MD) radiation, and present further constraints on the NS nuclear equation of state (EoS) via mass quadrupole deformation and r-mode fluid oscillations of the magnetar. We present two short GRBs with measured redshifts, 101219A and 160821B, whose X-ray light curves exhibit an internal plateau. This suggests that a supra-massive NS may survive as the central engine. By considering twelve NS EoSs, within the mass quadrupole deformation scenario we find that the GM1, DD2, and DDME2 models give an $M_{\rm p}$ band falling within the 2$\sigma$ region of the proto-magnetar mass distribution for $\varepsilon=0.01$. This is consistent with the constraints from the MD radiation dominated model of rotational energy loss. However, for an r-mode fluid oscillation model with $\alpha=0.1$ the data suggest that the NS EOS is close to the Shen and APR models, which is obviously different from the MD radiation dominated and mass quadrupole deformation cases., Comment: 7 pages, 1 table, and 4 figures. ApJ in press, matched the published version

GRB 200219A is a short gamma-ray burst (GRB) with an extended emission (EE) lasting $\sim 90$s. By analyzing data observed with the {\em Swift}/BAT and {\em Fermi}/GBM, we find that a cutoff power-law model can adequately fit the spectra of the initial short pulse with $\rm E_{p}=1387^{+232}_{-134}$ keV. More interestingly, together with the EE component and early X-ray data, it exhibits plateau emission smoothly connected with a $\sim t^{-1}$ segment and followed by an extremely steep decay. The short GRB composed of those three segments is unique in the {\em Swift} era and is very difficult to explain with the standard internal/external shock model of a black hole central engine, but could be consistent with the prediction of a magnetar central engine from the merger of an NS binary. We suggest that the plateau emission followed by a $\sim t^{-1}$ decay phase is powered by the spin-down of a millisecond magnetar, which loses its rotation energy via GW quadrupole radiation. Then, the abrupt drop decay is caused by the magnetar collapsing into a black hole before switching to EM-dominated emission. This is the first short GRB for which the X-ray emission has such an intriguing feature powered by a magnetar via GW-dominated radiation. If this is the case, one can estimate the physical parameters of a magnetar, the GW signal powered by a magnetar and the merger-nova emission are also discussed., Comment: 21 pages, 2 Tables, 7 figures, ApJL in press, two more references added, matched the published version

Attached microalgae cultivation could simplify the microalgae harvest process and reduce its associated cost, which has attracted much attention recently. In this paper, the reactor patterns, advantages, microalgae biomass productivity, influencing factors and the microalgae physicochemical properties in the attached microalgae cultivation was summarized to show the sketch of this novel microalgae cultivation. It was concluded that the attached microalgae cultivation is advantageous in achieving less water and space consumption, higher water treatment potential and higher biomass productivity compared with the traditional suspended microalgae cultivation. The accumulation of the attached microalgae biomass showed a linear increase with culture time with the largest productivity up to 20.7 g m−2 d−1. Chlorella and Scenedesmus were the top two species that have been studied in attached culture system. Cellulose acetate/nitrate membrane, polycarbonate membrane and cotton were the most popular materials used owing to their high hydrophilicity and wide availability. Flow rate, nutrients, light, CO2 and other factors could affect the attached microalgae productivity and the physicochemical property in a way different from the suspended microalgae, which were described in detail in this review. At last, some technical bottlenecks and the corresponding solvents in the attached microalgae were suggested.

Void space clogging of horizontal subsurface flow constructed wetlands (HSSF CWs) can result in hydraulic malfunction and reduced service life. In this study, the effect of solids accumulation and root growth on the hydrodynamics of the HSSF CWs was evaluated by tracer tests. The experimental HSSF CWs were designed to be unplanted, uniformly planted and linearly planted to investigate the role of Iris (I. pseudacorus) root and the effects of different planting patterns. Results showed that the traditional design of HSSF CW was easy to develop the preferential bottom flow, and the solids accumulation near inlet zone aggravated the fast bottom flow throughout the whole systems. Plant root impeded the upper layer flow at the initial stage and promoted the uniform flow in the severe clogging situation. Consequently, the water flow was more uniform in the linearly planted (dispersion number (d) = 0.16) and uniformly planted (d = 0.13) systems than the unplanted system (d = 0.10) at the final stage. The opening of new void spaces by root growth and the upward water drag induced by plant transpiration was responsible for such phenomenon.

Microalgae are promising feedstocks for food, feed, fuel, fertilizers, fine chemicals and other value-added products, and are considered of great potential in wastewater treatment. Convenient acquisition of the cultivated microalgal biomass or easy separation of microalgae from the treated effluents, however, are the prerequisites of these applications. Biomass harvesting and dewatering in traditional suspended cultivation systems are costly and energy intensive, since volumes of liquid medium needs to be separated from the obtained diluted microalgal biomass. Attached systems can effectively overcome such limitations by introducing substratum into the liquid medium for microalgae attachment, and the attached biomass with magnitudes higher solid content can be collected via simple and straightforward harvesting methods such as mechanical scraping. As an emerging field with less than ten years of research history, systematic information on microalgae attachment and attached systems for biomass production and wastewater treatment has not been thoroughly reviewed. This review, therefore, analyzed the two-step mechanisms of microalgae attachment, discussed the influencing factors of attached microalgal growth including properties of substratum, properties of microalgal cells, turbulence of liquid medium, frequency of biomass harvesting, etc., and their possible impacts on microalgal biofilm formation and biomass production in attached systems. Classification of attached microalgal systems based on different criteria such as substratum orientation, relative position of microalgal cells and the culturing medium, system scales and culturing medium, was summarized. Performances of attached microalgal systems for biomass production and wastewater treatment were evaluated, with commonly used parameters identified and compared as well. Recommendations for future theoretical research and practical applications were also addressed in the manuscript. This review could offer much useful information on microalgal attachment and attached systems for biomass production and wastewater treatment, and may serve as guidance for future studies in related fields.

A newly-born magnetar is thought to be central engine of some long gamma-ray bursts (GRBs). We investigate the evolution of the electromagnetic (EM) emission from the magnetic dipole (MD) radiation wind injected by spin-down of a newly-born magnetar via both quadrupole gravitational-wave (GW) and MD radiations. We show that the EM luminosity evolves as $L_{\rm em}\propto (1+t/\tau_c)^{\alpha}$, and $\alpha$ is $-1$ and $-2$ in the GW and MD radiation dominated scenarios, respectively. Transition from the GW to MD radiation dominated epoch may show up as a smooth break with slope changing from $-1$ to $-2$. If the magnetar collapses to a black hole before $\tau_c$, the MD radiation should be shut down, then the EM light curve should be a plateau followed by a sharp drop. The expected generic light curve in this paradigm is consistent with the canonical X-ray light curve of {\em Swift} long GRBs. The X-ray emission of several long GRBs are identified and interpreted as magnetar spin-down via GW or MD, as well as constrain the physical parameters of magnetar. The combination of MD emission and GRB afterglows may make the diversity of the observed X-ray light curves. This may interpret the observed chromatic behaviors of the X-ray and optical afterglow light curves and the extremely low detection rate of a jet-like break in the X-ray afterglow light curves of long GRBs., Comment: 7 pages, 2 figures, Accepted for publication in MNRAS

The long-term contamination of soil by microplastics may pose risks that are often still not well understood, and the ecological effects of microplastics are mainly dependent on their environmental behavior in environments. This study used saturated quartz sand as a solid porous medium to study the migration and influencing factors of 40–48 μm polyethylene (PE) particles in saturated porous media. The breakthrough curves at different injection concentrations (0.3, 0.4, 0.5 mg/L), flow rates (1.0, 1.5, 2.0, 2.5 ml/L), porous medium particle sizes (1–2, 2–4 mm), ionic strengths (0, 0.01, 0.05 mol/L) and concentrations of fulvic acid (FA) (0, 5, 10 mg/L) were compared and analyzed. The Derjaguin-Landau-Verwey-Overbeek (DLVO) theory was used to more accurately explain relevant transport behaviors. The results showed that the input concentration, flow rate, and particle size can affect the migration of PE particles individually or in combination. As ionic strength increased, the repulsion between microplastics and quartz sand gradually disappeared according to DLVO theory, and their attraction gradually strengthened. As a result, fewer microplastics could penetrate the sand column and reach the water body. With the continuous addition of FA, the repulsive energy between microplastics and quartz sand rose from DLVO theory, and the migration ability of microplastics initially increased before becoming stable because of the effect of straining. In all cases, the migration ability of PE was low (C/C0

Microalgae are potential candidates for the production of valuable products, such as renewable biodiesel, health products and pigments. However, low biomass productivity has restricted their large-scale applications. In this study, the effects of two auxins (one natural type of indole-3-acetic acid (IAA) and the other synthetic type of 2,4-dichlorophenoxyacetic acid (2,4-D)) on the growth and fatty acid methyl esters (FAMEs) production of a freshwater microalgae Scenedesmus sp. LX1 were investigated. Both auxins showed a "low dosage-promotion and high dosage-inhibition" effect on the growth and FAMEs accumulation. The optimum dosage of IAA and 2,4-D were 1mgL-1 and 0.1mgL-1, respectively. Moreover, the IAA could increase the monounsaturated fatty acid content. The auxins may promote the growth by enhancing the photosynthetic activity through increasing chlorophyll contents. Therefore, auxin significantly enhanced microalgal growth and FAMEs accumulation, and has a potential for application in developing efficient microalgal cultivation.

Heterotrophic cultivation of Chlorella pyrenoidosa based on straw substrate was proposed as a promising approach in this research. The straw pre-treated by ammonium sulfite method was enzymatically hydrolyzed for medium preparation. The highest intrinsic growth rate of C. pyrenoidosa reached to 0.097 h−1 in hydrolysate medium, which was quicker than that in glucose medium. Rising nitrogen concentration could significantly increase protein content and decrease lipid content in biomass, meanwhile fatty acids composition kept stable. The highest protein and lipid content in microalgal biomass reached to 62% and 32% under nitrogen excessive and deficient conditions, respectively. Over 40% of amino acids and fatty acids in biomass belonged to essential amino acids (EAA) and essential fatty acids (EFA), which were qualified for high-value uses. This research revealed the rapid biomass accumulation property of C. pyrenoidosa in straw hydrolysate medium and the effectiveness of nitrogen regulation to biomass composition at heterotrophic condition.

Pharmaceuticals and personal care products (PPCPs) cause ongoing water pollution and consequently have attracted wide attention. Constructed wetlands (CWs) show good PPCP removal performance through combined processes of substrates, plants, and microorganisms; however, most published research focuses on the role of substrates and microorganisms. This review summarizes the direct and indirect roles of wetland plants in PPCP removal, respectively. These direct effects include PPCP precipitation on root surface iron plaque, and direct absorption and degradation by plants. Indirect effects, which appear more significant than direct effects, include enhancement of PPCP removal through improved rhizosphere microbial activities (more than twice as much as bulk soil) stimulated by radial oxygen loss and exudate secretions, and the formation of supramolecular ensembles from PPCPs and humic acids from decaying plant materials which improving PPCPs removal efficiency by up to four times. To clarify the internal mechanisms of PPCP removal by plants in CWs, factors affecting wetland plant performance were reviewed. Based on this review, future research needs have been identified.

Inspired by plant transpiration, an attached microalgae−simulated plant system was designed to enhance the transpiration of reverse osmosis concentrated water (i.e., WROC) and realize the conversion of pollutants to microalgae biomass. The results showed that the production rate of clean water could reach as high as 14.84 L·m−2·day−1, which was significantly influenced by the humidity of the air and the growth status of the attached microalgae. Moreover, the enhancement of water evaporation by microalgae was more obvious under relatively low humidity. Pollutants, transported along with the water, could transform into microalgae biofilm or crystallize at the top of the microfiber. TN and TP transformation into biomass resources were maximized in 40% diluted WROC, with efficiencies of 60.91% and 38.49%, respectively. Of note, the accumulation of phosphorus in the micro-environment of attached microalgae may inhibit microalgal growth in the later stages of cultivation, owing to the relatively low movability. Hence, this system could be applied for high-efficiency wastewater purification, especially under high humidity. Wastewater dilution and periodic microalgae harvest could guarantee the attached microalgae growth and increase the pollutant-bioresource conversion rate.

Sodium borosilicate glasses are candidate materials for high-level radioactive waste vitrification; therefore, understanding the irradiation effects in model borosilicate glass is crucial. Effects of electronic energy deposition and nuclear energy deposition induced by the impact of heavy ions on the hardness and Young’s modulus of sodium borosilicate glass were investigated. The work concentrates on sodium borosilicate glasses, henceforth termed NBS1 (60.0% SiO2, 15.0% B2O3, and 25.0% Na2O in mol%). The NBS1 glasses were irradiated by P, Kr, and Xe ions with 0.3 MeV, 4 MeV, and 5 MeV, respectively. The hardness and Young’s modulus of ion-irradiated NBS1 glasses were measured by nanoindentation tests. The relationships between the evolution of the hardness, the change in the Young’s modulus of the NBS1 glasses, and the energy deposition were investigated. With the increase in the nuclear energy deposition, both the hardness and Young’s modulus of NBS1 glasses dropped exponentially and then saturated. Regardless of the ion species, the nuclear energy depositions required for the saturation of hardness and Young’s modulus were apparent at approximately 1.2 × 1020 keV/cm3 and 1.8 × 1020 keV/cm3, respectively. The dose dependency of the hardness and Young’s modulus of NBS1 glasses was consistent with previous studies by Peuget et al. Moreover, the electronic energy loss is less than 4 keV/nm, and the electronic energy deposition is less than 3.0 × 1022 keV/cm3 in this work. Therefore, the evolution of hardness and Young’s modulus could have been primarily induced by nuclear energy deposition.

Binary neutron star (NS) mergers may result in remnants of supra-massive or even stable NS, which have been supported indirectly by observed X-ray plateau of some gamma-ray bursts (GRBs) afterglow. Recently, Xue et al. (2019) discovered a X-ray transient CDF-S XT2 that is powered by a magnetar from merger of double NS via X-ray plateau and following stepper phase. However, the decay slope after the plateau emission is a little bit larger than the theoretical value of spin-down in electromagnetic (EM) dominated by losing its rotation energy. In this paper, we assume that the feature of X-ray emission is caused by a supra-massive magnetar central engine for surviving thousands of seconds to collapse black hole. Within this scenario, we present the comparisons of the X-ray plateau luminosity, break time, and the parameters of magnetar between CDF-S XT2 and other short GRBs with internal plateau samples. By adopting the collapse time to constrain the equation of state (EOS), we find that three EOSs (GM1, DD2, and DDME2) are consistent with the observational data. On the other hand, if the most released rotation energy of magnetar is dominated by GW radiation, we also constrain the upper limit of ellipticity of NS for given EOS, and it is range in $[0.32-1.3]\times 10^{-3}$. Its GW signal can not be detected by aLIGO or even for more sensitive Einstein Telescope in the future., 13 pages, 5 figures,1 table. Accepted for publication by Research in Astronomy and Astrophysics

Cross-sections of the (n,2n) reactions for neodymium (Nd) isotopes induced by 14 MeV neutrons were measured in this work by using the activation and relative methods. The measured cross-sections of the 150Nd(n,2n)149Nd, 148Nd(n,2n)147Nd, and 142Nd(n,2n)141Nd reactions were 1854 ± 81, 1789 ± 119, and 1559 ± 98 mb, respectively, at a neutron energy of 14.2 ± 0.2 MeV, and 1485 ± 74, 1726 ± 85, and 1670 ± 119 mb, respectively, at 14.9 ± 0.2 MeV. The results were compared with the experimental values from the reported literature, with the evaluated data from the ENDF/B-VII.1, CENDL-3.1, and JENDL-4.0 libraries, and with the curves calculated by the Talys-1.8 code.

The event GW170817/GRB 170817A, discovered via the successful joint observation of its gravitational wave radiation and its multi-wavelength electromagnetic counterparts, was the first definite "smoking-gun" from the merger of two neutron stars (NSs). However, the remnant of the merger remains unknown. Piro et al. recently claimed that a low-significance X-ray variability in GRB 170817A. By systematically comparing the properties of variability in the afterglow of GRB 170817A and X-ray flares in GRB afterglows, we find that this X-ray variability seems to share similar statistical correlations with X-ray flares in GRB afterglows. We further investigate several possible merger product scenarios to see whether they can produce the observed X-ray variability in GRB 170817A. The first scenario invokes a stable magnetar as the central engine producing the later X-ray variability via differential rotation or fall-back accretion onto the NS. The second scenario invokes a black hole as the central engine with a fall-back accretion process. The final scenario is a central engine with a long-lived supra-massive NS. We find that the first two scenarios have difficulty producing the later X-ray variability, which requires either an impractical NS magnetic field or an extraordinarily large stellar envelope and an extremely long accretion timescale. However, the third scenario seems to be consistent with observations, and the later X-ray variability can be produced by the magnetosphere which is expelled following the collapse of the NS with a $B_p\in(3.6, 13.5)\times10^{13}$ G., Comment: 7 pages, 2 figures. Accepted for publication in MNRAS

Since low oxygen supplement rate in subsurface flow constructed wetland was a critical obstacle to bacterial nitrification process, a novel tidal unsaturated constructed wetland (CWTU) realized by the siphon effluent pipe was proposed in this study to overcome the problem and strengthen the pollutants removal. Four CWTUs were constructed with different ratios of tidal unsaturated section to constant saturated section (80: 0, 60: 20, 50: 30 and 30: 50). Results showed that the NH4+-N removal efficiency increased with the increasing ratio of tidal unsaturated section to constant saturated section. Among which, CWTU1 significantly enhanced the oxygen supplement from air to wastewater, and the subsequent removal efficiencies of NH4+-N (51.04 ± 6.99 % in CWTU1), and COD (89.90 ± 2.10 % in CWTU1) compared with traditional wetland (NH4+-N 12.96 ± 5.26 %, COD 65.30 ± 10.08 %) (p

Morusin, a prenylated flavone isolated from Morus species, was treated as a potential anti-tumor drug since it inhibited effects on numerous types of human cancer cells. In some sense, drugs-related metabolites always contribute to pharmacological changes, inducing therapy improvement, reduced efficacy, or side-effects. Thus, the characterization of metabolites and the potential functions exerted great importance in clinical applications. Till now, the metabolism feature of morusin was still unclear, and its pharmacological changes were still unconducted, too. In this work, an integrated strategy based on metabolites profiling and network pharmacological was applied to characterize the metabolism feature and reveal pharmacological changes of morusin in vivo. As a result, a total of 31 metabolites (19 in plasma, 8 in urine, 30 in feces, 6 in heart, 17 in liver, 4 in spleen, 6 in lung, 6 in kidney, and 3 in brain) were screened out in rats, and 11 of them were characterized for the first time. Among them, metabolites M6, M18, M19, M20, and M28, were the main metabolites. Phase I metabolic reactions of hydroxylation, dehydrogenation (cyclization), isomerization and phase II reactions of glucuronidation occurred, and glucuronidation and hydroxylation were the two main metabolic reactions. Moreover, the pharmacological difference between morusin and five main metabolites was predicted by an network pharmacological method, and these 6 candidates targeted 177 targets. Meanwhile, in addition to common pathways (PI3K-Akt signaling pathway, proteoglycans in cancer, hepatitis B, cAMP signaling pathway, and viral carcinogenesis) of morusin in cancer, six metabolites’ targets were involved in prostate cancer, chemokine signaling pathway, ras signaling pathway and neuroactive ligand-receptor interaction, indicating that these functional changes might result in novel pharmacological mechanism and new indications. Our work provided the metabolism feature and functional modifications of morusin in vivo for the first time, and meaningful information for further pharmacological validations or potential indications in clinic were supplied.

The partially unsaturated constructed wetland was demonstrated to be able to enhance the oxygen supplement for the microbial nitrification. However, the fast gravity flow of wastewater on the smooth surface of substrate in unsaturated zone led to a short contact time between wastewater and biofilm on the surface of substrate for the microbial pollutant oxidation process. While, the strengthened oxygen supplement also consumed organic carbon, intensifying the shortage of electron donator for the denitrification process. To further enhance the efficiency of both nitrification and denitrification processes, two strategies were conducted as follows: (1) adding microfiber in unsaturated zone to extend the hydraulic retention time (HRT) and improve the oxygenating efficiency; (2) adding slow-release carbon source (Poly butylenes succinate, PBS) as electron donor in saturated zone for denitrification. Results showed that the ammonia oxidation efficiency reached up to 97.0% in the microfiber-enhanced constructed wetland. Additionally, adding microfiber provided more sites for microbes and increased the total number of microbes in unsaturated zone. The addition of PBS in the saturated zone obviously improved the denitrification efficiency with the total nitrogen (TN) removal rate raising from 20.6 ± 4.0% to 90.4 ± 2.7%, which excellently solved the problem of poor denitrification efficiency caused by low ratio of carbon to nitrogen (C/N). In conclusion, the association of microfiber and PBS in partially unsaturated constructed wetland finally accomplished the thorough nitrogen removal.

The role of bacteria/extracellular polymeric substances (EPS) coated carriers on attached microalgae growth in suspended-solid phase photobioreactor (sspBR) was assessed in this study. The results showed that pre-coating cotton with ambient bacteria and their EPS improved the attached microalgal growth by as much as 230% in terms of attached microalgae density. Additionally, the single cell dry weight, chemical composition and oxygen evolving activity of attached microalgae were significantly affected by the presence of bacteria/EPS coating on the cotton carriers. The protein content of microalgae cells cultivated in the ssPBRs with carriers coated by bacteria and sterilized bacteria were on average 26% and 15% more than uncoated carriers, respectively. Through absorbing and immobilizing nutrients from the bulk medium, the bacteria/EPS coating provided the attached microalgae with nitrogen/phosphorus for protein synthesis, especially during the late stages of batch cultivation.

Nowadays, microalgae-based bioenergy has been commonly considered as a potential substitute of the non-renewable traditional energy resources with increasing attention. Nevertheless, microalgal cells will secrete/release some Soluble Algal Products (SAPs) into the culture medium during microalgal biomass cultivation. Studies on the influences, especially the negative ones, of SAPs in large-scale cultivation of microalgae for biomass/bioenergy production remain limited. The negative effects, properties, production and treatments of SAPs were therefore investigated and summarized in this paper. It was found that SAPs may inhibit microalgal growth, raise the cost of microalgal recovery and lower the performance of the downstream discharged water treatment process after microalgal cultivation, among others. The production of SAPs could be as high as 70 mg/L in terms of dissolved organic carbon (DOC), causing substantial environmental issues. The chemical properties of SAPs significantly varied among different microalgal species, growth phases and nutrient concentrations. The distribution of molecular weight of SAPs varied from less than 1 kDa to more than 100 kDa, meanwhile the hydrophilic/hydrophobic property of SAPs showed a consistency. All studies showed that more than 60% of SAPs are hydrophilic. After comparing three different SAPs treatment methods, it was concluded that biodegradation could be a feasible and economical process to remove SAPs based on the properties of SAPs. The mechanisms of SAPs formation and the mitigation of their undesirable effects are still not clear. Therefore, a systematic and deep study on those topics is urgently needed.

During growth microalgal cells release soluble algal products (SAPs), which may be associated with negative effects on microalgal cultivation, including inhibiting microalgal growth, inducing bacterial growth, etc. In this study, the SAP production characteristics of Scenedesmus sp. LX1 under different light intensities, cultivation temperatures and initial pH were investigated. Under the cultivation conditions which were adverse to microalgal growth, including low light intensity (1000 lx), low temperature (15 °C) and extreme pH (5 or 11), microalgal cells tended to release more SAPs. At the end of cultivation, the concentration of SAP changed in the range of 4.9–14.3 mg DOC L − 1 , with the SAP production yield per cell of 0.5–4 pg DOC cell − 1 except under the initial pH of 5. SAP production rate of Scenedesmus sp. LX1 increased with microalgal growth rate, but was limited by algal density. During growth, the major constitutes of SAPs changed from growth-associated products into non-growth-associated products gradually.

An intense D-T/D-D neutron generator is currently being developed at the Lanzhou University. The Cockcroft–Walton accelerator, as a part of the neutron generator, will be used to accelerate and transport the high-current low-energy beam from the duoplasmatron ion source to the rotating target. The design of a high-current low-energy beam transport (LEBT) line and the dynamics simulations of the mixed beam were carried out using the TRACK code. The results illustrate that the designed beam line facilitates smooth transportation of a deuteron beam of 40 mA, and the number of undesired ions can be reduced effectively using two apertures.

Evidence for the central engine of gamma-ray bursts (GRBs) has been collected in the Neil Gehrels \emph{Swift} data. For instance, some GRBs show an internal X-ray plateau followed by very steep decay, which is difficult to be interpreted within the framework of a black hole (BH) central engine, but are consistent within a rapidly spinning magnetar engine picture. The very steep decay at the end of the plateau suggests a sudden cessation of the central engine, which is explained as the collapse of a supra-massive magnetar into a black hole when it spins down. Here we propose that some additional evidence, such as a second X-ray plateau feature would show up, if the fall-back accretion could activate the newborn BH and sufficient energy could be transferred from the newborn BH to the GRB blast wave. With a systematic data analysis for all long GRBs, we find three candidates in \emph{Swift} sample, i.e., GRBs 070802, 090111, and 120213A, whose X-ray afterglow light curves contain two plateaus, with the first one being an internal plateau. We find that in a fairly loose and reasonable parameter space, the second X-ray plateau data for all 3 GRBs could be well interpreted with our proposed model. Future observations are likely to discover more similar events, which could offer more information of the properties of the magnetar as well as the newborn BH., accepted for publication by ApJ

A tidal unsaturated constructed wetland (CWTU) was proposed to improve the oxygen supplement and ammonia oxidation without high energy consumption of aeration. A siphon effluent tube was imported in CWTU to form the tidal discharge, expose part substrate into air, and supply oxygen to the biofilm and the thin wastewater film on the surface of substance during wastewater treatment process. The results showed that NH4+-N removal rates in CWTUs ranged from 42.76% to 61.17%, much higher than that in conventional non-aeration CWNA (25.25%). The addition of hydrophilic material and COD in CWTU further improved nitrogen removal from 24.72% to 30.92%. Seen from the spatial distribution of pollutants and the relative functional gene abundance of bacteria community, it could be concluded that the tidal unsaturated section near the inlet played an important role in nitrogen removal. Increasing organic carbon supply was proposed to improve the denitrification function of the constant saturated section of CWTUs.

The presence of sufficient dissolved oxygen (DO) in a constructed wetland (CW) is vital to the process of removing ammonia nitrogen and organics from wastewater. To achieve total nitrogen removal, which is characterised by enhanced ammonia nitrogen removal, this study offers an efficient strategy to increase the oxygen supply by establishing constant unsaturated zones and baffles in simulating constructed wetlands (SCWs). Henceforth, this strategy is addressed as a partially unsaturated SCW. A centrally located high tube was set up inside the wetland to create an unsaturated zone at a higher level. The effectiveness of the unsaturated zone to supplement the oxygen content was evaluated by comparing with controls (an unaerated SCW and an aerated SCW). The results show the chemical oxygen demand removal rate (85 ± 6%) in the partially unsaturated SCW was equivalent to that in the aerated SCW (83 ± 6%), while the ammonia nitrogen removal rate was 11 times higher compared to that of the unaerated SCW. The removal potential of the partially unsaturated SCW under different HRT (hydraulic retention time)s (12, 24, and 36 h) was examined, and the 36 h-SCW performed the best in the removal of organics and nitrogen. The mechanisms behind the unsaturated zone strategy were studied by analysing water and microbe samples along the pathway. The results from the water quality indicators and the quantitative polymerase chain reactions along the pathway showed the unsaturated zone contributed to the removal of primary organics and ammonia nitrogen. The superior performance of unsaturated zone strategy was discussed further using the enrichment of ammonia-oxidising bacteria, mass of oxygen uptake, and baffle design. The results indicate that the amoA gene/16s rRNA gene abundance ratio and the oxygen uptake (336 ± 44 g m−3 d−1) in the partially unsaturated SCW was higher than that observed in the two controls.

Constructed wetland (CW) for wastewater treatment has attracted increasing attention. In this review, the system configuration optimization, purification effect and general mechanisms of nitrogen removal in CW are systematically summarized and discussed. Ammonia oxidation is a crucial and primary process for total nitrogen (TN) removal in domestic or livestock wastewater treatment. Aeration, waterdrop influent and tidal operation are three main methods to strengthen the oxygen supplement and nitrification process in CW. Aeration significantly increases the ammonia removal rate (almost 100%), followed by the removal of chemical oxygen demand (COD) and TN. Solid carbon source, iron and anode material can be filled as electron donor for the denitrification process. The co-adjustment of oxygen and carbon/electron donor can form different conditions for different nitrogen removal pathways (e.g. the simultaneous nitrification-denitrification, the partial nitrification-denitrification and the anammox process), and achieve the optimal removal of nitrogen.

The favored progenitor model for Gamma-ray Bursts (GRBs) with Supernova (SN) association is the core collapse of massive stars. One possible outcome of such a collapse is a rapidly spinning, strongly magnetized neutron star (>magnetar>). We systematically analyze the multi-wavelength data of GRB/SN associations detected by several instruments before 2017 June. Twenty GRB/SN systems have been confirmed via direct spectroscopic evidence or a clear light curve bump, as well as some spectroscopic evidence resembling a GRB-SN. We derive/collect the basic physical parameters of the GRBs and the SNe, and look for correlations among these parameters. We find that the peak brightness, Ni mass, and explosion energy of SNe associated with GRBs are statistically higher than other Type Ib/c SNe. A statistically significant relation between the peak energy of GRBs and the peak brightness of their associated SNe is confirmed. No significant correlations are found between the GRB energies (either isotropic or beaming-corrected) and the supernova energy. We investigate the energy partition within these systems and find that the beaming-corrected GRB energy of most systems is smaller than the SN energy, with less than 30% of the total energy distributed in the relativistic jet. The total energy of the systems is typically smaller than the maximum available energy of a millisecond magnetar (2 × 10 erg), especially if aspherical SN explosions are considered. The data are consistent with - although not proof of - the hypothesis that most, but not all, GRB/SN systems are powered by millisecond magnetars.© 2018. The American Astronomical Society. All rights reserved., We thank J. D. Lyman for sharing the Type Ib/c SNe data with us, Wei-Hua Lei for helpful discussion, and the anonymous referee for helpful comments. D.A.K. acknowledges financial support from the Spanish research project AYA 2014-58381-P, and from Juan de la Cierva Incorporacion fellowship IJCI-2015-26153. This work is supported by the National Basic Research Program (973 Programme) of China 2014CB845800, the National Natural Science Foundation of China (grant Nos. 11603006, 11851304, 11533003, and U1731239), Guangxi Science Foundation (grant Nos. 2017GXNSFFA198008, 2016GXNSFCB380005, 2015GXNSFDA139002, and AD17129006), the One-Hundred-Talents Program of Guangxi colleges, the high-level innovation team and outstanding scholar program in Guangxi colleges, Scientific Research Foundation of Guangxi University (grant No. XGZ150299), and special funding for Guangxi distinguished professors (Bagui Yingcai & Bagui Xuezhe)

Two-episode emission components separated by quiescent gaps in the prompt emission of gamma-ray bursts (GRBs) have been observed in the {\em Swift} era, but there is a lack of spectral information due to the narrow energy band of the {\em Swift}/Burst Alert Telescope. In this paper, a systematic analysis of the spectral and temporal properties of the prompt emission of 101 {\em Fermi}/Gamma-ray Burst Monitor detected long GRBs show the existence of two-episode emission components in the light curves, with quiescent times of up to hundreds of seconds. We focus on investigating the differences of those two emission episodes. We find that the light curves of the two emission components exhibit different behavior, e.g., a soft emission component that either precedes or follows the main prompt emission or that the intensity of the two emission episodes are comparable with each other. No statistically significant correlation in the duration of the two emission episodes can be claimed. We define a new parameter $\varepsilon$ as the ratio of the peak flux of the first and second emission episodes and find that a higher $\varepsilon$ corresponds to a larger fluence. The preferred spectral model in our analysis is a cutoff power-law model for most GRBs. The distribution of $E_p$ for episodes I and II range from tens of keV to 1000 keV with a lognormal fit and there are no significant differences between them. Moreover, we do not find significant relationships between $\varepsilon$ and $E_p$ for the two emission episodes. Those results suggest that these two-episode emission components likely share the same physical origin., Comment: 22 pages, 1 Table, 5 Figures. updated the reference list and matched the published version

Attached microalgae cultivation for the algae-based products is considered as a promising approach to simplify biomass recovery processes and reduce the cost. However, as an incipient research field, biomass accumulation is the mainly index for attached microalgal growth evaluation. To break through such limitations, physiological properties of attached microalgae (e.g. the oxygen evolving activity and the main organic composition of cells), which are important for microalgal growth evaluation but are still unclear in most studies, were studied using an attached microalgae culture system, i.e. suspended-solid phase photobioreactor (ssPBR) in this paper. As light, nutrients and other environmental conditions of attached microalgae were different from the suspended microalgae, physiological properties of attached microalgae also varied from the suspended ones. Besides the relatively lower biomass accumulation rate, attached microalgae also had a lower oxygen evolving activity (65% on average) comparing to suspended microalgae. The composition of microalgae changed towards accumulating more protein when suspended microalgae turned to attached status. The relative protein content of attached microalgae (50.1% ± 10.1%) was approximately 30% higher than the suspended algae (36.0% ± 16.1%) on average. The discovery of physiological properties of attached microalgae in this paper could help the production of high-protein microalgae-related products and explain some phenomenon during the production of microalgae-related products.

Knowledge of actinides (n,f) fission process induced by neutron is of importance in the field of nuclear power and nuclear engineering, especially for reactor applications. In this work, fission characteristics of 238U(n,f) reaction induced by D-T neutron source were simulated with Geant4 code from multiple perspectives, including the fission production yields, total nubar, kinetic energy distribution, fission neutron spectrum and cumulative γ-ray spectrum of the fission products. The simulation results agree well with the experimental nuclear reaction data (EXFOR) and evaluated nuclear data (ENDF). Mainly, this work was to examine the rationality of the parametric nuclear fission model in Geant4 and to direct our future experimental measurements for the cumulative fission yields of 238U(n,f) reaction.

The fission cross-section of the 232Th(n,f)141Ba reaction induced by neutrons around 14MeV was measured precisely with the neutron activation and off-line gamma-ray spectrometric technique. Neutron fluence was monitored on-line using the accompanying $\alpha$ -particles from the 3H(2H,n)4He reaction, whereas the neutron energies were measured by the method of cross-section ratios of 90Zr(n,2n)89Zr to 93Nb(n,2n)92mNb reactions. The experimentally determined 232Th(n,f)141Ba reaction cross-sections were $12.2\pm 0.4$ mb at $E_{n} = 14.1 \pm 0.3$ MeV, 13.0±0.5 mb at $E_{n} = 14.5 \pm 0.3$ MeV and 13.3±0.5 mb at $E_{n} = 14.7 \pm 0.3$ MeV, respectively.