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3 results on '"Frédéric Véran"'

1. Multimethod Approach for Extensive Characterization of Gallnut Tannin Extracts

Author

Christine Le Guernevé, Frédéric Véran, Pascale Williams, François Garcia, Céline Poncet-Legrand, Hélène Hallé, Aude A. Watrelot, Véronique Cheynier, Bertrand Robillard, Emmanuelle Meudec, Sciences Pour l'Oenologie (SPO), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Oenologique de Champagne (IOC), European Union (EU), and Région Grand-Est

Subjects
Magnetic Resonance Spectroscopy, Antioxidant, 1H DOSY NMR, medicine.medical_treatment, 1H 2D 1H/13C, gel permeation chromatography, Mass spectrometry, 01 natural sciences, Gel permeation chromatography, Quercus, Tandem Mass Spectrometry, enological tannins, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, Nuts, Tannin, 31P NMR, [CHIM]Chemical Sciences, Chromatography, High Pressure Liquid, UPLC−DAD−ESI−MS, Wine, chemistry.chemical_classification, Chromatography, Plant Extracts, 010405 organic chemistry, Chemistry, polygalloylglucose, 010401 analytical chemistry, General Chemistry, molecular weight distribution, Hydrolyzable Tannins, antioxidant properties, 0104 chemical sciences, Heteronuclear molecule, Molar mass distribution, Composition (visual arts), General Agricultural and Biological Sciences, gallotannins
Abstract

International audience; Gallotannins extracted from gallnuts are commonly added to wine to improve its properties. They consist of mixtures of galloylester derivatives of glucose. However, their composition and properties are not well-established. In this study, methods based on liquid chromatography coupled to ultraviolet–visible detection and mass spectrometry, size-exclusion chromatography, and one-dimensional (31P) and two-dimensional (1H diffusion ordered spectroscopy, 31P total correlated spectroscopy, and 1H/13C heteronuclear single-quantum correlation and heteronuclear multiple-bond correlation) nuclear magnetic resonance spectroscopies have been implemented for extensive chemical characterization of three commercial gallnut tannin extracts. Differences in the proportions of the different constituents (gallic, digallic, and trigallic acids and galloylglucose derivatives) and in the structure and molecular weight distributions of gallotannins were demonstrated between the three extracts, with chains containing 8.5, 12.2, and 12.4 galloyl groups on average for TAN A, TAN B1, and TAN B2, respectively. The antioxidant capacities of the extracts, evaluated using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) method, were similar and related mostly to their total tannin content, with only a limited impact of the tannin composition.

Published
2020
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2. Dissecting genetic architecture of grape proanthocyanidin composition through quantitative trait locus mapping

Author

Yung-Fen Huang, Frédéric Véran, Loïc Le Cunff, Agnès Doligez, Cécile Morel, Yves Bertrand, Nancy Terrier, Alexandre Fournier-Level, Valérie Miralles, Véronique Cheynier, Aurélie Canaguier, Jean-Marc Souquet, Patrice This, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Department of Ecology and Evolutionary Biology, Brown University, Géno-vigne® (UMT Géno-vigne®), Institut National de la Recherche Agronomique (INRA)-Institut Français de la Vigne et du Vin (IFV)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unité de recherche en génomique végétale (URGV), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), Architecture et Fonctionnement des Espèces Fruitières [AGAP] (AFEF), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
0106 biological sciences, Candidate gene, [SDV]Life Sciences [q-bio], Quantitative Trait Loci, Plant Science, Berry, Biology, Quantitative trait locus, 01 natural sciences, Anthocyanidin reductase, 03 medical and health sciences, lcsh:Botany, Proanthocyanidins, Vitis, Cultivar, Allele, 030304 developmental biology, 2. Zero hunger, Genetics, 0303 health sciences, Chromosome Mapping, food and beverages, Genetic architecture, lcsh:QK1-989, Epistasis, Research Article, 010606 plant biology & botany
Abstract

Background Proanthocyanidins (PAs), or condensed tannins, are flavonoid polymers, widespread throughout the plant kingdom, which provide protection against herbivores while conferring organoleptic and nutritive values to plant-derived foods, such as wine. However, the genetic basis of qualitative and quantitative PA composition variation is still poorly understood. To elucidate the genetic architecture of the complex grape PA composition, we first carried out quantitative trait locus (QTL) analysis on a 191-individual pseudo-F1 progeny. Three categories of PA variables were assessed: total content, percentages of constitutive subunits and composite ratio variables. For nine functional candidate genes, among which eight co-located with QTLs, we performed association analyses using a diversity panel of 141 grapevine cultivars in order to identify causal SNPs. Results Multiple QTL analysis revealed a total of 103 and 43 QTLs, respectively for seed and skin PA variables. Loci were mainly of additive effect while some loci were primarily of dominant effect. Results also showed a large involvement of pairwise epistatic interactions in shaping PA composition. QTLs for PA variables in skin and seeds differed in number, position, involvement of epistatic interaction and allelic effect, thus revealing different genetic determinisms for grape PA composition in seeds and skin. Association results were consistent with QTL analyses in most cases: four out of nine tested candidate genes (VvLAR1, VvMYBPA2, VvCHI1, VvMYBPA1) showed at least one significant association with PA variables, especially VvLAR1 revealed as of great interest for further functional investigation. Some SNP-phenotype associations were observed only in the diversity panel. Conclusions This study presents the first QTL analysis on grape berry PA composition with a comparison between skin and seeds, together with an association study. Our results suggest a complex genetic control for PA traits and different genetic architectures for grape PA composition between berry skin and seeds. This work also uncovers novel genomic regions for further investigation in order to increase our knowledge of the genetic basis of PA composition.

Published
2012
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3. The oxidation catalytic converter reduces the inhibitory activity of soluble organic fractions of diesel particles on intercellular communication

Author

Philippe Pornet, Isabelle Vanrullen, Catherine Chaumontet, Paule Martel, Frédéric Véran, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV]Life Sciences [q-bio], 010501 environmental sciences, medicine.disease_cause, 01 natural sciences, Cell junction, 03 medical and health sciences, Diesel fuel, medicine, Environmental Chemistry, Carcinogen, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, Chemistry, business.industry, Gap junction, General Chemistry, CANCER, [SDV] Life Sciences [q-bio], Cell culture, Biophysics, Particle, RAT, Telecommunications, business, Intracellular, Genotoxicity
Abstract

The genotoxicity of diesel particles has been widely documented, and their tumor promoting effect has been reported recently using the gap junction intercellular communication (GJIC) assay. In the study, the ability of soluble organic fractions (SOF) of diesel particles emitted from cars equipped (WC) or not (WoutC) with the oxidation catalytic converter (OCC) and of particle SOF from an outdoor high polluted place (OHPP) to inhibit GJIC has been evaluated with two cell lines: a rat liver epithelial cell line (REL cells) and a rat pulmonary alveolar type II cell line (3T cells). With both cell lines, the results demonstrate that GJIC is strongly inhibited by WoutC, whereas it is much less reduced by WC ones: for REL cells, the activity of WC particles is 1/4 off the one of WoutC. Also, the authors show that the inhibition induced by WoutC is associated with a change in the GJ protein localization. Their results clearly show the effectiveness of the OCC technology in reducing both the tumor promoting activity and the genotoxicity of diesel particle SOF. (A)

Published
2000

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