1. Silicon-substituted rhodamines for stimulated emission depletion fluorescence nanoscopy
- Author
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Haidan Zhu, Ning Wang, Dazhi Zhang, Xiaoyan Cui, Yumeng Hao, Xiaowei Feng, and Ting Wang
- Subjects
Fluorescence-lifetime imaging microscopy ,Materials science ,Silicon ,STED microscopy ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Rhodamines ,chemistry ,Microscopy ,Stimulated emission ,0210 nano-technology ,Cell permeability - Abstract
As an essential part in the toolbox of super-resolution microscopy, stimulated emission depletion (STED) nanoscopy has been widely explored in revealing the substructure and bioactivities in fluorescence imaging. Among the applied STED fluorophores, silicon-substituted rhodamines (SiRs) belong to one of the most extensively employed fluorophores. The carboxy-SiR was favored in STED bioimaging with many advantages, including reliable photostability, cell permeability, tunable fluorogenicity, feasible structural decoration and so on. We reviewed the research of carboxy-SiR in the STED nanoscopy and hopefully this can inspire more efforts in the design and application of STED fluorophores.
- Published
- 2022