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Objective To observe the effect of curcumin on DNA methyltransferase (Dnmts) activity and tumor suppressor gene human H-cadherin (CDH13) in non-small-eell lung cancer i NSCLC A549 cells and to explore the mechanism of curcumin s demethylation. Methods A549 cells were divided into two groups: the observation group and the control group. Cells in the observation group were cultured with 40 μmol/ L curcumin and the culture medium, while cells in the control group only with the culture medium. MTT assay was used to detect the cell proliferation, and RT-PCR was used to detect the expression of CDH13 mRNA. CpG island methylation status of CDH13 promoter was detected by methylation-specific PCR (MSP) . and catalytic activity of Dnmts was detected by enzyme colorimetric determination kit. Results The OD of the observation group was 0. 638 ±0. 184. which was lower than that (0. 859 ±0. 040) of the control group (P < 0.05). The relative expression level of CDH13 mRNA in the observation group was 0.64 ±0.09. which was higher than that (0.43 ±0. 12) of the control group. There were methylation amplification bands, but no demethylation bands in the control group, and there were both methylation amplification bands and demethylation bands in the observation gmup. In the observation group and the control group, the activities of Dnmtl were separately (0. 153 ±0.016) and (0. 770 ± 0.014) μmol/min, and the activities of Dnmt3b were (0. 137 ±0.020) and (0. 179 ±0.013) μmol/min, respectively. The activities of Dnmtl and Dnmt3b in the observation group were lower than those in the control group (all P < 0. 05). Conclusion Curcumin inhibits the methylation status of CDH13 by decreasing the activity of Dnmts. and thereby inhibits A549 cell proliferation. [ABSTRACT FROM AUTHOR]

Objective: To study the relationship between circulating tumor cells （CTC） and serum scc-ag and keratin 19 soluble fragment（cy-fra21-1） levels in patients with non-small cell lung cancer （NSCLC）. Methods: 100 NSCLC patients admitted to our hospital from February 2017 to February 2019 were included in the study, which was referred to as NSCLC group. Another 100 patients with benign lung lesions admitted to our hospital at the same time were taken as the benign lesion group. Another 100 healthy volunteers who underwent physical examination in our hospital at the same time were selected as the control group. The CTC and serum scc-ag and cy-fra21-1 levels of the three groups were detected and compared, and the correlation between CTC and serum scc-ag and cy-fra21-1 levels was analyzed by Pearson correlation. The diagnostic efficacy of CTC and serum scc-ag and cy-fra21-1 levels in NSCLC was analyzed by ROC curve. In addition, the relationship between CTCS, serum scc-ag, cy-fra21-1 and lymph node metastasis in NSCLC patients was analyzed. Results: The levels of CTC, serum scc-ag and cy-fra21-1 in the NSCLC group and the benign lesion group were higher than those in the control group, and the levels of CTC, serum scc-ag and cy-fra21-1 in the NSCLC group were higher than those in the benign lesion group （all P<0.05）. Pearson correlation analysis showed that there was a positive correlation between CTC and serum scc-ag and cy-fra21-1 levels in NSCLC patients （r=0.642、0.601, P<0.05）. According to ROC curve analysis, the combined detection of CTC, serum scc-ag and cy-fra21-1 levels in the diagnosis of NSCLC showed that the area under the curve, the sensitivity and the specificity were all higher than those measured separately （all P<0.05）. The levels of CTC and serum scc-ag and cy-fra21-1 in patients with NSCLC lymph node metastasis were higher than those in patients without lymph node metastasis （all P<0.05）. Conclusion: There is a significant positive correlation between CTCS and serum scc-ag and cy-fra21-1 levels in NSCLC patients, and the combined detection of the three can improve the diagnostic value of NSCLC, and the above three indicators are closely related to lymph node metastasis. [ABSTRACT FROM AUTHOR]