Search

[Objective] Screening and obtaining the antagonistic actinomycetes with significant antifungal activity against the pathogen of Sarcandra glahra, for the theoretical foundation for biological control of anthracnose. [Method] The actinomycetes were isolated by root soil of health S. glabra using spread plate method, and the highest antifungal activity of strain was screened by the method of plate confrontation, and using in vitro leaf stabbing wound inoculation method to screen the control effect of antagonistic strain against anthracnose of S. glabra. Moreover, the species was identified through morphological characterization and 16 S rDNA sequence by establishing a phylogenetic tree to analyze its classification status. [Result] By plate confrontation and antibacterial spectrum test, the results showed that the JT-2 F was isolated from soil had the highest antagonistic effect against anthracnose of S. gfubra, resulting in the hyphae being swelled and deformed, the antagonism rate was 86. 75%. Antibacterial spectrum tests showed it had antagonistic activities to 14 plant pathogens, whose average inhibition rate was 81. 50%. Based on morphological characters and 16S rDNA sequence analysis, strain JT-2F was identified as Streptomyces tsukiyonensis. In vitro control effect test results showed that the fermentation broth of JT-2F strain had 87. 32 % control effect on anthracnose of S. glabra. [Conclusion ] JT-2F has development and application potential as biocontrol material for anthracnose of S. glabra. [ABSTRACT FROM AUTHOR]

【Objective】The diversity of endophytic fungi of medicinal plants of Sarcandra glabra was analyzed,and the strains with strong antifungal activity were screened to lay a foundation for the search for new antimicrobial resources.【Method】Endophytic fungi with strong antimicrobial activities were isolated from different tissues of S. glabra by plate confrontation method,and their species was identified by combining morphological characteristics and ITS sequence to screen out endophytic fungi with strong antimicrobial activities.【Result】One hundred and eleven of various endophytes were obtained from the different organizations of S. glabra,forty-two strains of different morphological types were selected for molecular identification,which belonged to nine orders and twenty genera,twenty-eight species. The RJ-1 and JJ-1 had remarkable antimicrobial activities to fifteen pathogenic fungi. Morphological and molecular identification showed that RJ-1 and JJ-1 were Phomopsis heveicola and Neofusicoccum parvum,respectively. Their antifungal activity to seven pathogens including Botrytis sp.,Fusarium solani,Colltetorrichum gloeosporioides of Panax notoginseng,Colltetorrichum sp. of Musa,Phoma sp. of Blumea balsamifera,Alternaria sp. of Picria felterrae,Colletotrichum dematium of S. glabra was over 80 %,with strong antifungal broad-spectrum.【Conclusion】The endophytic fungi in S. glabra were diverse and rich. Some strains such as RJ-1 and JJ-1 deserve further research as an alternative antimicrobial resource. [ABSTRACT FROM AUTHOR]

The inhibition rates of biocontrol fungus F2 on main pathogenic fungi of Panax notoginseng including Alternaria panax, Colletotrichum gloeosporioides and Fusarium solani were tested in this study. The effects of strain F2 on the mycelia growth of the 3 pathogenic fungi were also observed under the microscope. The results showed that strain F2 could inhibit A. panax, C. gloeosporioides and F. solani obviously with the high inhibition rates of 88. 67 %, 91. 00 % and 75. 76 % respectively. When observed under the microscope, mycelia growths of the 3 pathogenic fungi were affected obviously by strain F2. The mycelia of A. panax and C. gloeosporioides showed swollen, abnormal and cracked. And the mycelia of F. solani showed cracked. Base on the morphological and molecular biology characteristics analysis, strain F2 was identified as Trichoderma asperellum. [ABSTRACT FROM AUTHOR]