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Objective To investigate the expression changes of histone deacetylase 7（HDAC7） and ubiquitin-specific peptidase 10（USP10） in non-small-cell lung cancer （NSCLC） tissues and their significance. Methods Ninety-eight NSCLC patients underwent tumor resection, with cancer tissues and adjacent tissues preserved during the operation. Immunohistochemistry was used to detect the expression of HDAC7 and USP10 in tissues. Spearman correlation analysis was used to analyze the correlation between HDAC7 and USP10 expression in NSCLC cancer tissues, and to analyze the relationships between HDAC7 and USP10 expression and the clinicopathological parameters of NSCLC patients. Kaplan-Meier survival curve was used to analyze the relationship between HDAC7 and USP10 expression and the prognosis of NSCLC patients, and the COX multivariate proportional hazard model was used to analyze the factors for the prognosis of NSCLC patients. Results The positive rates of HDAC7 in the NSCLC tissues and adjacent tissues were 65. 31% （64/98） and 6. 12% （6/98), respectively （χ2 =74. 756, P<0. 05). The positive rates of USP10 in the NSCLC tissues and adjacent tissues were 63. 27% （62/98） and 8. 16% （8/98), respectively （χ2 =64. 800, P<0. 05). The positive expression rates of HDAC7 and USP10 in cancer tissues with stage III NSCLC, low tumor differentiation, and lymph node metastasis were higher than those with TNM stage Ⅰ–Ⅱ, high and medium differentiation, and no lymph node metastasis （all P<0. 05). The expression of HDAC7 and USP10 in NSCLC cancer tissues showed a significant positive correlation （r=0. 714, P<0. 05). The 3-year cumulative survival rates of HDAC7 positive and USP10 positive patients were lower in comparison with those of HDAC7 negative and USP10 negative patients （ χ2 =16. 300, 15. 870, respectively； all P<0. 05). Positive HDAC7 expression, positive USP10 expression, poorly differentiated tumor, lymph node metastasis, and TNM stage III were independent risk factors for the prognosis of NSCLC patients. Conclusion HDAC7 and USP10 are highly expressed in NSCLC tissues, HDAC7 and USP10 may synergistically promote the development and progression of NSCLC, and the prognosis of NSCLC patients with high expression levels of HDAC7 and USP10 is poor. [ABSTRACT FROM AUTHOR]

目的 比较整合素 α3（ITGA3）在三种肺癌细胞株 A549（肺腺癌）、H1650（肺泡细胞癌）、DMS53（小细胞肺癌）中的表达。方法　应用基因芯片技术提取 A549、H1650、DMS53细胞株的mRNA，反转录为cDNA，以32 P标记cDNA，与整合素基因芯片进行杂交，经放射自显影后读取灰度值，并以基因表达的限制性分析（ RAGE）和流式细胞仪技术进行验证。结果 ITGA3亚单位在A549、H1650、DMS53细胞株中的表达分别为7．58±1．37、8．10±1．51、2．38±0．20，A549、H1650与 DMS53细胞株比较，P 均＜0．01。 RAGE 结果显示，经 AvaⅡ酶切后，A549、H1650、DMS53细胞株均出现ITGA3（69 bp），分别为167．0、172．1、44．7，A549、H1650与DMS53细胞株比较，P均＜0．01。流式细胞仪检测结果显示，ITGA3在A549、H1650、DMS53细胞株中的表达分别为83．62±1．40、90．86±4．78、1．24±0．53，A549、H1650与DMS53细胞株比较，P均＜0．01。结论 ITGA3在A549和H1650细胞株中的表达上调，高于其在DMS53细胞株中的表达。 [ABSTRACT FROM AUTHOR]

The incidence and mortality of lung cancer ranked the first in China. China had 787,000 new cases of lung cancer in 2015, and a majority of these patients with advanced lung cancer, With the development and popularization of high-resolution computed tomography, more and more early-stage lung adenocarcinomas are found in screening. The imaging finding of early-stage lung adenocarcinomas often manifests as part solid nodule (PSN) containing ground glass opacity (GGO). Although the imaging manifestation of the nodules can't accurately predict the pathologic type of nodules, the parts of solid nodule and GCO still have some pathologic indications, and the prognostic evaluation effect of the maximum diameter of PSN is superior to that o the whole nodule. With the development of the molecular radiography and molecular pathology, the relationship of imaging manifestation of the PSN and metastasis were focused on. Some PSNs with special nature are more active and rapidly progressed than the pure GGOs. While compared to the pure solid nodules, the aggressiveness of PSNs are insufficient, with lower metastatic rates of lymph node and better prognoses. Currently, international acknowledge recommends to take active intervention measure for PSN which are highly suspected to be maligunt. We focus on the diagnosis and treatment of PSNs, systemically depict their staging, follow-up, surgical treatment, gene detection and immunotherapy. [ABSTRACT FROM AUTHOR]