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The experiment was to investigate the mechanism of improvement and protective effect of sodium acetate on MAC-T cell steatosis by adding different concentrations of sodium acetate to bovine MAC-T cell steatosis model. MAC-T cells were stimulated by LPS to establish a steatosis model of MAC-T cells. Control group, LPS treatment group (cells were stimulated by 1 000 μg/L LPS for 9 h) and sodium acetate + LPS treatment group with different concentrations (2, 4 and 8 mmol/L sodium acetate were added to LPS treatment group, respectively) were established. The content of TG and the expression levels of ACC, FAS, SCD-1, CPT-1, CPT-2 and ACO of key genes of lipid anabolic metabolism were determined, and the content of TNF-α, IL-6 and IL-8 were detected. The results showed that after LPS stimulation for 9 h, the TG content of MAC-T cells was extremely decreased (P<0.01), and the total lipid drop area was significantly decreased (P<0.05). Compared with control group, the TG content of cells in LPS treatment group was significantly decreased (P<0.05), and the mRNA expression levels of ACC, FAS and SCD-1 genes related to lipid anabolic metabolism were extremely decreased (P<0.01). The mRNA expression levels of CPT-1, CPT-2 and ACO related to lipid catabolism were significantly up-regulated (P<0.05), the content of inflammatory factors TNF-α, IL-6 and IL-8 were significantly increased (P<0.05). Compared with LPS treatment group, the mRNA expression levels of ACC, FAS and SCD-1 were significantly up-regulated after adding 4 mmol/L sodium acetate (P<0.05), CPT-1 was significantly down-regulated (P<0.05), and the content of TNF-α and IL-6 were significantly decreased (P<0.05). TG content was significantly increased (P<0.05). After adding 8 mmol/L sodium acetate, the mRNA expression levels of ACC and FAS were significantly up-regulated (P<0.05), the mRNA expression levels of CPT-1 and CPT-2 were significantly down-regulated (P<0.05), and the content of TNF-α, IL-6 and IL-8 were significantly decreased (P<0.05). TG content was significantly increased (P<0.05). The study indicates that 4, 8 mmol/L sodium acetate can promote the lipid anabolic metabolism of MAC-T cells, promote TG synthesis, and have a certain protective effect on the inflammatory damage of MAC-T cells. [ABSTRACT FROM AUTHOR]