1. Nogo-B与JCAD诱导肝癌基因激活.
- Author
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郭阵雨, 赵新军, and 马军仁
- Abstract
Based on Hill kinetics and Michaelis - Menten function, we investigate Nogo - Band JCAD inducing activation of hepatocellular carcinoma(HCC) gene. The theoretical model considers : (1) Nogo - B promotes the degradation of oxidized low density lipoprotein (ox LDL) by interacting with Autophagy - related 5 gene (ATG5), and the degraded oxLDL(DoxLDL) enhances the Yes - associated protein (YAP) activity in Hippo signaling pathway, which activates the expression of oncogenes(connective tissue growth factor (CTGF)) by promoting the production of a large amount of Lysopho - sphatidic acid (LPA); (2) JCAD interacts with large tumor suppressor homolog 2 (LATS2) to regulate phosphorylation of YAP. Dephosphorylated YAP activates tumor - related proliferation factors in the Hippo signaling pathway, in turn upregulating activation of YAP to promote HCC cell proliferation. We found that ox LDL degrades in a short time. The degradation of oxLD L promotes LPA largely, and then promotes the activation of YAP in Hippo signaling pathway. During this process, DoxLDL will quickly activate a large number of inactivated cascade path signals ( ( Lysophosphatidylcholine ( LPC), auto- taxin( ATX), LPA, etc. ), which activate expression of HCC gene. By analyzing the temporal evolutions of the levels of Nogo - B, it can be found that Nogo - Band ATG5 forms a complex NA. The ATG5 amplifies the signal of Nogo - B expression. It makes Nogo - B - enhanced activation expression, which promotes LPA production and activates YAP. A pulsed signal driven by the CEBPJ3 will also enhance the transcriptional activation of Nogo - B. The YAP and CTGF showed a steady state with the change of Nogo - B. This indicates further that oxLDL degradation induced by up - regulation of Nogo - B expression activates the stable oxLDL - Nogo - B - YAP - CTGF pathway. Hippo signaling is highly activated after ectopic Nogo - B expression. By analyzing the regulatory effects with the JACD and kinase LATS2, we found that JACD inhibits YAP phosphorylation, which greatly increases the expression level of CTGF. It is opposite to the effect of JCAD regulation, LATS2 promotes YAP phosphorylation, and then decreases the expression of CTGF and other proliferation factors. Our theoretical results are consistent with the experimental observations, and provide a fundamental understanding of the Non - alcoholic steatohepatitis ( NASH) inducing HCC [ABSTRACT FROM AUTHOR]
- Published
- 2022
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