[Objectives] The paper aims to analyze the rice chalkiness character and create materials for studying the molecular mechanisms of rice chalkiness in rice quality breeding. [Methods] In the present study a set of 71 advanced backcrossing population was constructed using high quality and low chalky variety’Koshihikarias’donor parent,high yield and high chalky variety’Guichao 2’as recurrent parent by continuous backcrossing and molecular marker assisted selection. The chalkiness phenotypes including the percentage of grains with chalkiness(PGWC)and degree of percentage of grains with chalkiness(DPGWC)were investigated and the quantitative trait loci(QTL)of advanced backcross population were detected in 2014 Nanjing and 2015 Hainan,respectively. [Results] The results showed that PGWC and DPGWC were significantly associated,and thirty-three PGWC QTL were detected in more than one year. Among them five could be detected repeatedly in two different environments. In addition,seventeen DPGWC QTL were detected in two different environments,three of them can be detected repeatedly,among them two are coincided with PGWC sites. Moreover,seven pleiotropic QTL had the same impacts on the PGWC and DPGWC. [Conclusions] Through two years of phenotype and molecules data of chalky trait,50 QTL were detected,and seven were detected in both two years at Nanjing and Hainan. In addition,the controlling PGWC QTL qPGWC4.1,qPGWC4,qPGWC7.1 and the controlling DPGWC QTL qDPGWC4.1,qDPGWC8.1,qDPGWC10.1 might be new findings in this study. [ABSTRACT FROM AUTHOR]