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Objective: To investigate the expression and clinical significance of co-signal molecule B7-H3 in non-small cell lung cancer (NSCLC) cell lines and tissues. Methods: B7-H3 expression level was evaluated by RT-PCR and western blot in five NSCLC cell lines. Specimens from 121 participants were stained immunohistochemically. Staining was quantified using Image-Proplus software, and integrated optical density was measured on each section. Results: The constitutive expression of B7-H3 in all five cell lines was found. The expression of B7-H3 were higher than that in benign lesions and non-cancer tissues respectively (P<0.001). B7-H3 expression in well differentiated NSCLC was Inhigher than that in not well differentiated one (P<0.05).There was a significant association between expression of B7-H3 and lymph node metastasis. Conclusion: B7-H3 is overexpressed in NSCLC tissues and may be an important target for diagnosis or the therapy of the disease. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of vimentin in non-small cell lung cancer (NSCLC) tissues and the relationship with invasion and metastasis of lung cancer. Methods: Collected 150 cases of NSCLC carcinoma tissues and 79 cases of adjacent normal tissues (>5cm away from carcinoma tissues) from the resection underwent in our hospital from June 2012 to June 2014, then extracted RNA of two groups, detected the expression of vimentin mRNA by real time-polymerase chain reaction (RT-PCR) method, and detected the expression of vimentin protein by immunohistochemical method, finally, analyzed the relationship between the expression of vimentin protein and the lymph node metastasis and TNMstage. Results: The expression of vimentin mRNA expression in NSCLC carcinoma tissues was significantly higher than adjacent normal tissues(P<0.05). The up-regulation expression of vimentin mRNA was related with lymph node metastasis and TNMstage (P<0.05). Conclusions: The expression of vimentin is up-regulation in NSCLC, which is closely related to the occurrence and invasion of NSCLC. [ABSTRACT FROM AUTHOR]