Objective To understand the current infection rate of nosocomial infection and use of antimicrobial agents in a children's hospital, and to provide scientific basis for the formulation of infection prevention and control measures. Methods Using a cross sectional survey approach, bedside surveys were combined with access to medical records to investigate hospitalized children from 0,00 to 24, 00 on July 23,2018. Results A total of 2427 hospitalized children were investigated, with an actual rate of 100.00%. There were 65 cases of nosocomial infection,the prevalence of nosocomial infection rate was 2.68%,68 cases were infected, and the infection rate was 2. 80%. The department with the highest prevalence of nosocomial infections was department of hematology oncology (23. 44%), followed by the department of thoracic surgery and surgical intensive care unit. The lower respiratorytract (48. 53%) and upper respiratory tract (26. 47%) were the main infection sites. The top 3 hospital infection pathogens were Klebsiella pneumoniae,Acinetobacter baumannii and Staphylococcus epidermidis. Among the 2 427 hospitalized children, 1 245 were antibacterial drugs, the antibacterial drug use rate was 51. 30%, mainly treated with drugs (90. 52%), the therapeutic + preventive drug ratio was 5. 62%,and the combined antibacterial drug use rate was 83. 78%, The proportion of the two drugs was 15. 66%, and the proportion of the triple and above drugs was 0. 56%. Before the treatment of antibiotics (including treatment + preventive drugs), the prevalence rate was 62. 66%. Conclusion It is necessary to strengthen the routine monitoring of the high infection department and high infection site, and use antibiotics rationally to ensure the safety of children. [ABSTRACT FROM AUTHOR]