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Aims. To analyse the impact of electromagnetic oscillations in the sound range on diffusion the 3.25 % solution of sodium hypochlorite in the dentine of the tooth root.Materials and methods. A pilot study on diffusion of 3.25% of solution of sodium hypochlorite with dye fuchsine red was executed on 100 extracted teeth of the person. The control group was made by 40 teeth, the introduction of an antiseptic was conducted without use of sound waves. The main group of 60 teeth was divided into 3 subgroups. For the purpose of intensifying of the penetration of an antiseptic in each subgroup, electromagnetic oscillations (frequency range around 20,000 Hz) were applied with exposition time respectively 1 minute, 2 minutes, 3 minutes.Results. Application of sound waves in the canal of a tooth root led to augmentation of penetration of an antiseptic in the system of dentinal canaliculus and microchannels of the tooth root dentine.Conclusions. This process took place most effectively in single-root teeth when exposed to sound vibrations, smaller efficiency is revealed in multirooted teeth that indicates the difference in physical properties of the tissues of the various groups of the teeth.