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Your search keyword '"Yong-Xing Ai"' showing total 3 results
Start Over Author "Yong-Xing Ai" Topic α-toxin
3 results on '"Yong-Xing Ai"'

1. Allicin Reduces the Production of α-Toxin by Staphylococcus aureus

Author

Xiao-Han Dai, Jia-Zhang Qiu, Xiao-Di Niu, Yu Zhang, Yong-Xing Ai, Xu-Ming Deng, Hong-En Li, Jian-Feng Wang, Jing Dong, Ming-Jing Luo, and Bing-Feng Leng

Subjects
Staphylococcus aureus, α-toxin, allicin, sub-inhibitory concentrations, Organic chemistry, QD241-441
Abstract

Staphylococcus aureus causes a broad range of life-threatening diseases in humans. The pathogenicity of this micro-organism is largely dependent upon its virulence factors. One of the most extensively studied virulence factors is the extracellular protein α-toxin. In this study, we show that allicin, an organosulfur compound, was active against S. aureus with MICs ranged from 32 to 64 μg/mL. Haemolysis, Western blot and real-time RT-PCR assays were used to evaluate the effects of allicin on S. aureus α-toxin production and on the levels of gene expression, respectively. The results of our study indicated that sub-inhibitory concentrations of allicin decreased the production of α-toxin in both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in a dose-dependent manner. Furthermore, the transcriptional levels of agr (accessory gene regulator) in S. aureus were inhibited by allicin. Therefore, allicin may be useful in the treatment of α-toxin-producing S. aureus infections.

Published
2011
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2. Allicin Reduces the Production of α-Toxin by Staphylococcus aureus

Author

Xiaohan Dai, Bingfeng Leng, Xiaodi Niu, Hongen Li, Yu Zhang, Jianfeng Wang, Yong-Xing Ai, Jiazhang Qiu, Mingjing Luo, Xuming Deng, and Jing Dong

Subjects
Methicillin-Resistant Staphylococcus aureus, Staphylococcus aureus, Transcription, Genetic, α-toxin, allicin, sub-inhibitory concentrations, Bacterial Toxins, Pharmaceutical Science, Virulence, Microbial Sensitivity Tests, Biology, medicine.disease_cause, Hemolysis, Article, Analytical Chemistry, Microbiology, lcsh:QD241-441, chemistry.chemical_compound, Hemolysin Proteins, Western blot, lcsh:Organic chemistry, Bacterial Proteins, Drug Discovery, medicine, Extracellular, Animals, Disulfides, Physical and Theoretical Chemistry, Staphylococcus aureus delta toxin, medicine.diagnostic_test, Allicin, Organic Chemistry, medicine.disease, Haemolysis, Sulfinic Acids, Anti-Bacterial Agents, chemistry, Chemistry (miscellaneous), Culture Media, Conditioned, Trans-Activators, Molecular Medicine, Rabbits
Abstract

Staphylococcus aureus causes a broad range of life-threatening diseases in humans. The pathogenicity of this micro-organism is largely dependent upon its virulence factors. One of the most extensively studied virulence factors is the extracellular protein α-toxin. In this study, we show that allicin, an organosulfur compound, was active against S. aureus with MICs ranged from 32 to 64 μg/mL. Haemolysis, Western blot and real-time RT-PCR assays were used to evaluate the effects of allicin on S. aureus α-toxin production and on the levels of gene expression, respectively. The results of our study indicated that sub-inhibitory concentrations of allicin decreased the production of α-toxin in both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in a dose-dependent manner. Furthermore, the transcriptional levels of agr (accessory gene regulator) in S. aureus were inhibited by allicin. Therefore, allicin may be useful in the treatment of α-toxin-producing S. aureus infections.

Published
2011
Full Text
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3. Allicin Reduces the Production of α-Toxin by Staphylococcus aureus.

Author

Bing-Feng Leng, Jia-Zhang Qiu, Xiao-Han Dai, Jing Dong, Jian-Feng Wang, Ming-Jing Luo, Hong-En Li, Xiao-Di Niu, Yu Zhang, Yong-Xing Ai, and Xu-Ming Deng

Subjects
STAPHYLOCOCCUS aureus infections, MICROBIAL virulence, MICROBIAL toxins, ORGANOSULFUR compounds, GENETIC regulation, HEMOLYSIS & hemolysins, WESTERN immunoblotting, REVERSE transcriptase polymerase chain reaction, THERAPEUTICS
Abstract

Staphylococcus aureus causes a broad range of life-threatening diseases in humans. The pathogenicity of this micro-organism is largely dependent upon its virulence factors. One of the most extensively studied virulence factors is the extracellular protein a-toxin. In this study, we show that allicin, an organosulfur compound, was active against S. aureus with MICs ranged from 32 to 64 μg/mL. Haemolysis, Western blot and real-time RT-PCR assays were used to evaluate the effects of allicin on S. aureus α-toxin production and on the levels of gene expression, respectively. The results of our study indicated that sub-inhibitory concentrations of allicin decreased the production of a-toxin in both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in a dose-dependent manner. Furthermore, the transcriptional levels of agr (accessory gene regulator) in S. aureus were inhibited by allicin. Therefore, allicin may be useful in the treatment of a-toxin-producing S. aureus infections. [ABSTRACT FROM AUTHOR]

Published
2011
Full Text
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