1. Astaxanthin-Loaded Nanostructured Lipid Carriers for Preservation of Antioxidant Activity
- Author
-
Rodriguez-Ruiz, Violeta, Salatti Dorado, José Ángel, Barzegari, Abolfazl, Nicolas-Boluda, Alba, Houaoui, Amel, Caballero-Casero, Noelia, Caballo Linares, Carmen, Sicilia Criado, María Dolores, Bastias, Jorge, Pauthe, Emmanuel, Omidi, Yadollah, Letourneur, Didier, Rubio Bravo, Soledad, Gueguen, Virginie, Pavon-Djavid, Graciela, Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe), Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT), Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 - Institut universitaire de formation des maîtres - Académie de Grenoble (UJF IUFM Grenoble), Université Joseph Fourier - Grenoble 1 (UJF), and Rodriguez-Ruiz, Violeta
- Subjects
0301 basic medicine ,Antioxidant ,Chemical Phenomena ,medicine.medical_treatment ,nanostructured lipid carriers ,Pharmaceutical Science ,02 engineering and technology ,Xanthophylls ,Microscopy, Atomic Force ,Antioxidants ,Analytical Chemistry ,chemistry.chemical_compound ,Drug Discovery ,Carotenoid ,ComputingMilieux_MISCELLANEOUS ,Lipophilic ABTS assay ,chemistry.chemical_classification ,Drug Carriers ,Molecular Structure ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences ,021001 nanoscience & nanotechnology ,Lipids ,3. Good health ,[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences ,astaxanthin ,antioxidants ,Chemistry (miscellaneous) ,α-TEAC ,Lipophilicity ,Molecular Medicine ,0210 nano-technology ,food.ingredient ,[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Haematoccocus pluvialis ,Oxidative phosphorylation ,Article ,lcsh:QD241-441 ,03 medical and health sciences ,food ,Dynamic light scattering ,lcsh:Organic chemistry ,Astaxanthin ,medicine ,Particle Size ,Physical and Theoretical Chemistry ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,lipophilic ABTS assay ,Nanostructured lipid carriers ,Chromatography ,Sunflower oil ,Organic Chemistry ,Nanostructures ,030104 developmental biology ,chemistry ,Xanthophyll - Abstract
Astaxanthin is a xanthophyll carotenoid showing efficient scavenging ability and represents an interesting candidate in the development of new therapies for preventing and treating oxidative stress-related pathologies. However, its high lipophilicity and thermolability often limits its antioxidant efficacy in human applications. Here, we developed a formulation of lipid carriers to protect astaxanthin&rsquo, s antioxidant activity. The synthesis of natural astaxanthin-loaded nanostructured lipid carriers using a green process with sunflower oil as liquid lipid is presented. Their antioxidant activity was measured by &alpha, Tocopherol Equivalent Antioxidant Capacity assay and was compared to those of both natural astaxanthin and &alpha, tocopherol. Characterizations by dynamic light scattering, atomic force microscopy, and scattering electron microscopy techniques were carried out and showed spherical and surface negative charged particles with z-average and polydispersity values of ~60 nm and ~0.3, respectively. Astaxanthin loading was also investigated showing an astaxanthin recovery of more than 90% after synthesis of nanostructured lipid carriers. These results demonstrate the capability of the formulation to stabilize astaxanthin molecule and preserve and enhance the antioxidant activity.
- Published
- 2018