1. The Polerovirus Minor Capsid Protein Determines Vector Specificity and Intestinal Tropism in the Aphid
- Author
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Catherine Reinbold, Véronique Ziegler-Graff, Kenneth Richards, Danièle Scheidecker, Monique Erdinger, E. Herrbach, Véronique Brault, Sophie Périgon, Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)
- Subjects
0106 biological sciences ,viruses ,PROTEIN ,BWYV ,01 natural sciences ,Polerovirus ,CUCURBIT APHID-BORN YELLOWS VIRUS ,GUT ,SPECIFICITY ,BEET WESTERN YELLOWS VIRUS ,0303 health sciences ,biology ,food and beverages ,APHID TRANSMISSION ,RELATION VIRUS-VECTEUR ,Virus-Cell Interactions ,3. Good health ,Intestines ,Capsid ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,VIRUS ,RTD ,POLEROVIRUSES ,food.ingredient ,Immunology ,Luteovirus ,Luteoviridae ,Microbiology ,APHIDIDAE ,Virus ,03 medical and health sciences ,food ,Species Specificity ,Virology ,Animals ,Tropism ,Plant Diseases ,030304 developmental biology ,CAPSID ,CABYV ,Beet western yellows virus ,fungi ,Midgut ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Insect Vectors ,Protein Structure, Tertiary ,TROPISM ,INTESTINAL TROPISM ,Aphids ,Insect Science ,Capsid Proteins ,010606 plant biology & botany - Abstract
Aphid transmission of poleroviruses is highly specific, but the viral determinants governing this specificity are unknown. We used a gene exchange strategy between two poleroviruses with different vectors, Beet western yellows virus (BWYV) and Cucurbit aphid-borne yellows virus (CABYV), to analyze the role of the major and minor capsid proteins in vector specificity. Virus recombinants obtained by exchanging the sequence of the readthrough domain (RTD) between the two viruses replicated in plant protoplasts and in whole plants. The hybrid readthrough protein of chimeric viruses was incorporated into virions. Aphid transmission experiments using infected plants or purified virions revealed that vector specificity is driven by the nature of the RTD. BWYV and CABYV have specific intestinal sites in the vectors for endocytosis: the midgut for BWYV and both midgut and hindgut for CABYV. Localization of hybrid virions in aphids by transmission electron microscopy revealed that gut tropism is also determined by the viral origin of the RTD.
- Published
- 2005
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