1. Surface glycoproteins of the recently identified African Henipavirus promote viral entry and cell fusion in a range of human, simian and bat cell lines
- Author
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Jan Felix Drexler, Philip Lawrence, Marcel A. Müller, Christian Drosten, Victor M. Corman, Beatriz Escudero Pérez, Viktor E. Volchkov, Bases moléculaires de la pathogénicité virale – Molecular Basis of Viral Pathogenicity (BMPV), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute of Virology, University of Bonn Medical Centre, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
African Henipavirus ,Cancer Research ,viruses ,Heterologous ,Membrane Fusion ,Virus ,Cercopithecus aethiops ,Cell Line ,03 medical and health sciences ,Viral Envelope Proteins ,Viral entry ,Henipavirus glycoproteins ,Virology ,Zoonoses ,Chiroptera ,Cricetinae ,Chlorocebus aethiops ,Animals ,Humans ,Hendra Virus ,Henipavirus ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Cell fusion ,biology ,030306 microbiology ,Virus Internalization ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Viral Tropism ,Infectious Diseases ,chemistry ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Host-Pathogen Interactions ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Glycoprotein ,Cellular Tropism - Abstract
International audience; The recent discovery of a wide range of henipavirus-like viruses circulating in Megabats in Africa raises the question as to the zoonotic potential of these pathogens given the high human mortality rates seen with their pathogenic relatives Nipah virus and Hendra virus. In the absence of cultured infectious African Henipavirus we have performed experiments with recombinant F and G glycoproteins from the representative African Henipavirus strain M74a aimed at estimating its cellular tropism and capacity to use similar receptors to its highly pathogenic counterparts. The ability of the M74a virus G surface protein to use the ubiquitous Ephrin B2 host cell receptor and its heterologous cross-compatibility with Nipah virus could be expected to impart upon this virus a reasonable potential for species spillover, although differences in fusion efficiency seen with the M74a virus F protein in certain cell lines could present a barrier for zoonotic transmission.
- Published
- 2013