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9 results on '"Jolivet, Pascale"'

1. How the shortest telomere in the cell signals senescence

Author

Berardi, Prisca, Mattarocci, Stefano, Langston, Rachel, Jolivet, Pascale, Charvin, Gilles, Doumic, Marie, Xu, Zhou, Teixeira, Maria Teresa, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Modelling and Analysis for Medical and Biological Applications (MAMBA), Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Jacques-Louis Lions (LJLL (UMR_7598)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire Jacques-Louis Lions (LJLL (UMR_7598)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), FRM: EQU202003010428, Ligue Contre Le Cancer, INCa : PLBIO16-059, EMBO, and ANR-11-LABX-0011,DYNAMO,Dynamique des membranes transductrices d'énergie : biogénèse et organisation supramoléculaire.(2011)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV]Life Sciences [q-bio], [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Abstract

International audience; When telomeres shorten, they are thought to lose their protective caps at a critical short length, activating the DNA damage response and recruiting DNA damage repair activities that would degrade, fuse or recombine dysfunctional telomeres. However, the structure(s) of short and dysfunctional telomeres, which respectively trigger permanent replicative senescence and/or potentially promote genome instability, remain unclear.To define the structure of telomeres at the point of dysfunction and the fate of cells carrying them, we have developed a system called FinalCut in Saccharomyces cerevisiae to induce a single telomere of defined length in cells in which we can conditionally inactivate telomerase. This allows 1/ the structural analysis of the shortest telomere in cells, 2/ the monitoring of the pace and the number of consecutive cell cycles from telomerase inactivation to cell death with our microfluidic system, and 3/ the combination of our single cell - single telomere resolved data with a mathematical model of senescence in budding yeast.Our results show that a very small fraction of cells can enter senescence in a probabilistic manner, as cells proliferate in the absence of telomerase. However, for the large majority of cells, when the shortest telomere reaches a critical threshold length, cells immediately stop proliferation. This is consistent with the model that the first telomere reaching a short threshold length is sufficient to abruptly stop cell divisions in a large majority of cells. The structure of this critically short telomere will be discussed.

Published
2022

2. The shortest telomere in the cell signals senescence through a probabilistic rather than deterministic mechanism

Author

Mattarocci, Stefano, Berardi, Prisca, Langston, Rachel, Jolivet, Pascale, Maes, Alexandre, Marcand, Stéphane, Doumic, Marie, Xu, Zhou, Teixeira, Maria Teresa, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Radiobiologie moléculaire et cellulaire (RMC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Modelling and Analysis for Medical and Biological Applications (MAMBA), Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Jacques-Louis Lions (LJLL (UMR_7598)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), INCa : PLBIO16-059, FRM: EQU202003010428, La Ligue Contre le Cancer, ANR-11-LABX-0011,DYNAMO,Dynamique des membranes transductrices d'énergie : biogénèse et organisation supramoléculaire.(2011), and ANR-16-CE12-0026,InTelo,Comment la prolifération cellulaire est contrôlée par les télomères: une approche à l'échelle de lignée cellulaire individuelle(2016)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, Telomeres, [SDV]Life Sciences [q-bio], DNA repair, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Telomerase
Abstract

International audience; Telomeres ensure genome integrity and are maintained by telomerase. During replicative senescence, telomerase is inactivated, telomere sequences progressively shorten, and set the limit for cell proliferation. As telomeres shorten, they are thought to lose their protective caps at a critical short length, activating the DNA damage response and recruiting DNA damage repair activities that would degrade, fuse, or recombine dysfunctional telomeres. However, the structure(s) of short and dysfunctional telomeres, which respectively trigger permanent replicative senescence or potentially promote genome instability, remain unclear. To define the structure of telomeres at the point of dysfunction and the fate of cells carrying them, we developed a system called FinalCut to induce a single telomere of defined length in cells in which we can conditionally inactivate telomerase. This allows structural analysis of this telomere, and combined with the use of our microfluidic system to track consecutive cell cycles from telomerase inactivation to cell death, we can achieve single telomere and single cell resolution. Our results show that the loss of telomere stability is progressive as the telomere shortens, and that telomeres of a given length can be protected or unprotected in separate cells. We conclude that telomere length is not the sole determinant of the switch between functional and dysfunctional telomeres. We therefore propose that the minimum length of telomeres to maintain cell viability follows a probabilistic, rather than deterministic, model.

Published
2021

3. Maturation of telomere 3'-overhangs is linked to the replication stress response

Author

Henninger, Erin, Jolivet, Pascale, Fallet, Emilie, Benmounah, Mohcen, Xu, Zhou, Mattarocci, Stefano, Teixeira, Maria Teresa, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Teixeira, Teresa

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Published
2020

4. Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants

Author

Eberhard, Stephan, Valuchova, Sona, Ravat, Julie, Fulneček, Jaroslav, Jolivet, Pascale, Bujaldon, Sandrine, Lemaire, Stéphane, Wollman, Francis-André, Teixeira, Maria Teresa, Riha, Karel, Xu, Zhou, Physiologie membranaire et moléculaire du chloroplaste (PMMC), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Gregor Mendel Institute of Molecular Plant Biology (GMI), Austrian Academy of Sciences (OeAW), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Base Sequence, [SDV]Life Sciences [q-bio], Genetic Variation, Telomere Homeostasis, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, macromolecular substances, Telomere, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Amino Acid Sequence, Telomerase, Research Articles, Chlamydomonas reinhardtii, Polymorphism, Restriction Fragment Length, Telomere Shortening, Research Article, Repetitive Sequences, Nucleic Acid
Abstract

This study characterizes the sequence, end structure, and length distribution of Chlamydomonas reinhardtii telomeres and shows that telomerase mutants are defective in telomere maintenance., Telomeres are repeated sequences found at the end of the linear chromosomes of most eukaryotes and are required for chromosome integrity. Expression of the reverse-transcriptase telomerase allows for extension of telomeric repeats to counteract natural telomere shortening. Although Chlamydomonas reinhardtii, a photosynthetic unicellular green alga, is widely used as a model organism in photosynthesis and flagella research, and for biotechnological applications, the biology of its telomeres has not been investigated in depth. Here, we show that the C. reinhardtii (TTTTAGGG)n telomeric repeats are mostly nondegenerate and that the telomeres form a protective structure, with a subset ending with a 3′ overhang and another subset presenting a blunt end. Although telomere size and length distributions are stable under various standard growth conditions, they vary substantially between 12 genetically close reference strains. Finally, we identify CrTERT, the gene encoding the catalytic subunit of telomerase and show that telomeres shorten progressively in mutants of this gene. Telomerase mutants eventually enter replicative senescence, demonstrating that telomerase is required for long-term maintenance of telomeres in C. reinhardtii.

Published
2019

5. Synchrotron X-ray footprinting – Mass-spectrometry to probe structure of proteins in interactions

Author

Gohon, Yann, Jolivet, Pascale, Froissard, Marine, Tâche, Roselyne, Chardot, Thierry, Giuliani, Alexandre, Mercere, Pascal, Vermachova, Martina, Młynarczyk, A. H., Daniel, Régis, Gonnet, Florence, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Synchrotron SOLEIL, Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering
Abstract

National audience; The meeting provides an invaluable forum for the synchrotron radiation user community, presenting an important opportunity to obtain the latest information on beamline performance at SOLEIL, to hear about the latest and most exciting results obtained at SOLEIL and to share scientific, technical and practical issues about the synchrotron radiation use.

Published
2015

6. Dynamic and structural studies of lipid droplets using synchrotron light

Author

Jamme, Frederic, Vindigni, Jean-David, Giuliani, Alexandre, Wien, Franck, Tâche, Roselyne, Jagic, Franjo, Réfrégiers, Mathieu, Jolivet, Pascale, Chardot, Thierry, Gohon, Yann, Froissard, Marine, Synchrotron SOLEIL, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Département Caractérisation et Elaboration des Produits Issus de l'Agriculture (CEPIA), and Institut National de la Recherche Agronomique (INRA)

Subjects
lipid droplets, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, synchrotron light, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Abstract

National audience; In yeast, animals and other organisms, storage lipids are maintained in the cytoplasm in specialized organelles called lipid droplets (LDs) or oil bodies. These structures consist mainly of a core of neutral lipids (triacylglycerols and/or steryl esters) enclosed in a monolayer of phospholipids, and contain a number of proteins which vary considerably with the species. In particular, some structural hydrophobic proteins stabilize the interface between lipid core and cellular aqueous environment (perilipins, apolipoproteins, oleosins). Oleosins, with their mass between 15 and 25 kDa, are the major proteins of plant LDs, and completely cover the LD surface. In the last decade, proteomic and genetic studies of this compartment have shown that LDs appear as a complex dynamic organelle with a role in metabolism control and cell signaling. LDs have received a lot of attention as their abnormal dynamics is associated with several metabolic diseases (obesity, diabetes, atherosclerosis and myopathies). In the context of green chemistry, LDs are also promising sources of neutral lipids for the development of derived products, such as biofuels and alternative molecules for food, medicine and cosmetics. For these reasons, understanding dynamics of LD and structure of LD integral proteins is of major importance. We developed a genetic approach using Saccharomyces cerevisiae to obtain heterologous expression of Arabidopsis thaliana LD proteins [1]: oleosin AtOle1 or caleosin AtClo1. These transformed yeasts overaccumulated LDs leading to a specific increase of storage lipids and these LDs harbor a high level of oleosins. These cells or their purified LDs were exposed to the Soleil synchrotron light to understand the dynamics of lipid storage and carbon fluxes by measuring the biochemical changes on single cells using the Synchrotron FTIR microspectroscopy on SMIS beamline [2]. to obtain structural data on whole cellular organelle and on oleosins inserted in LD using Sychrotron Radiation Circular Dichroism (SRCD) [3], Deep UV imaging on DISCO beamline and Synchrotron water radiolysis footprinting of accessible oleosin amino-acids on METROLOGY beamline.

Published
2014

7. Biocarburants, biomatériaux, chimie verte

Author

Froissard, Marine, Baumberger, Stéphanie, Enard, Corine, Dahuron, Lilian, Mouille, Gregory, Cintrat, Bernard, Canonge, Michel, Reymond, Matthieu, Jolivet, Pascale, Chardot, Thierry, Deruyffelaere, Carine, D'Andrea, Sabine, Institut Jean-Pierre Bourgin (IJPB), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech

Subjects
Biocarburants, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, biomatériaux, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, chimie verte, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2012

8. Développement durable et chimie verte

Author

Froissard, Marine, Enard, Corine, Baumberger, Stéphanie, Deruyffelaere, Carine, Cintrat, Bernard, Gohon, Yann, Jolivet, Pascale, Pouteaux, Marie, Méchin, Valérie, Zheng, Dan, Chardot, Thierry, Bouchez-Mahiout, Isabelle, D'Andrea, Sabine, Canonge, Michel, De Franssu, Bernard, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and Institut Polytechnique LaSalle Beauvais

Subjects
Développement durable, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, chimie verte, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2010

9. Carbone renouvelable et énergies vertes : la biomasse oléagineuse

Author

Chardot, Thierry, Jolivet, Pascale, Froissard, Marine, Canonge, Michel, Boulard, Céline, Miquel, Martine, Institut Jean-Pierre Bourgin (IJPB), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech

Subjects
énergies vertes, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Carbone renouvelable, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, biomasse oléagineuse, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

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