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Your search keyword '"Research institute of Fish Culture and Hydrobiology (RFC)"' showing total 5 results
Start Over Author "Research institute of Fish Culture and Hydrobiology (RFC)" Topic [sde.be]environmental sciences/biodiversity and ecology
5 results on '"Research institute of Fish Culture and Hydrobiology (RFC)"'

1. Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Author

Marc Suquet, Nelly Schmitt, Gaël Le Pennec, Jacky Cosson, Marina Demoy-Schneider, Ecosystèmes Insulaires Océaniens (UMR 241) (EIO), Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université de la Polynésie Française (UPF), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Research institute of Fish Culture and Hydrobiology (RFC), University of South Bohemia, Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Milt, endocrine system, CASA, Motility, Aquatic Science, Biology, Cryopreservation, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, Pinctada margaritifera, Andrology, 03 medical and health sciences, Human fertilization, Spermatozoa quality, reproductive and urinary physiology, Sperm motility, Margaritifera, urogenital system, ACL, 0402 animal and dairy science, 04 agricultural and veterinary sciences, biology.organism_classification, 040201 dairy & animal science, Sperm, Metabolism, 030104 developmental biology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J software and TEM, respectively. Sperm motility was assessed using Image J software combined with a computer assisted sperm analysis plugin adapted for assessing P. margaritifera spermatozoa. Other sperm quality parameters evaluated included O2 consumption, ATP content, and creatine kinase activity. Frozen/thawed spermatozoa exhibited damage to the head but retained a compact spherical shape. Sperm motility indicators showed a significant decrease in quality resulting from the freeze/thaw process. The percent of motile cells was 54% compared to 84% in fresh sperm, O2 consumption was 4.8 compared to 44 nanomol min−1, ATP content was 0.72 nmol/109 spermatozoa in the activating medium compared to 4.54 nmol/109 spermatozoa, and creatine kinase activity was 9.06 × 10−5 IU mg−1 protein compared to 12.5 × 10−5 IU mg−1 protein. The cryopreservation protocol allowed obtaining an acceptable motility rate after thawing, confirming the predictive value of sperm motility measurements before cryopreservation in terms of their ability to withstand freezing process.

Published
2018
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2. Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater

Author

Ian A.E. Butts, Mohammad Abdul Momin Siddique, Adrian D Macias, Otomar Linhart, Christian Fauvel, Research institute of Fish Culture and Hydrobiology (RFC), University of South Bohemia, Technical University of Denmark [Lyngby] (DTU), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), and Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, [SDE.MCG]Environmental Sciences/Global Changes, Receptivity, egg longevity, Aquatic Science, 01 natural sciences, reproductive ecology, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Animal science, Human fertilization, medicine, Dicentrarchus labrax, 14. Life underwater, Reproductive ecology, Sea bass, biology, 010604 marine biology & hydrobiology, 04 agricultural and veterinary sciences, biology.organism_classification, Mediterranean sea bass, Sperm, Fishery, medicine.anatomical_structure, embryonic structures, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Gamete, Dicentrarchus, Seawater, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, overripe eggs
Abstract

International audience; Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of pre-incubation time for both the fresh (P < 0.01) and overripe eggs (P < 0.01). Fertilization success of eggs significantly declined for both these treatments after 3 min of pre-incubation, which clearly indicates that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. This study has particular importance for understanding fertilization strategies, reproductive potential, as well as reproductive ecology of sea bass.

Published
2015
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3. Current conservation strategies for European crayfish

Author

Pavel Kozák, Julian D. Reynolds, Catherine Souty-Grosset, Antonín Kouba, Leopold Füreder, Research institute of Fish Culture and Hydrobiology (RFC), University of South Bohemia, Institute of Zoology and Limnology (UNIVERSITY OF INNSBRUCK), University of Innsbruck, Department of Chemistry [York, UK], University of York [York, UK], Ecologie, Evolution, Symbiose (EES), and Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers

Subjects
Astacus spp, 0106 biological sciences, Austropotamobius, Austropotamobius spp, Management, Monitoring, Policy and Law, Aquatic Science, 010603 evolutionary biology, 01 natural sciences, ark site, lcsh:Aquaculture. Fisheries. Angling, Indigenous, Ecosystem services, Stocking, Captive breeding, 14. Life underwater, Environmental planning, Nature and Landscape Conservation, Water Science and Technology, lcsh:SH1-691, Astacus, Ecology, biology, 010604 marine biology & hydrobiology, European Crayfish, biology.organism_classification, Crayfish, conservation management, restocking, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

A round table discussion involving 25 researchers from 11 European countries was organized during the congress “European Crayfish: food, flagships and ecosystem services” (held in Poitiers, France, 26–29th October 2010) to consider current activities dealing with management of indigenous crayfish species (ICS) with special regards to their culture, restocking and conservation strategy. In the following opinion paper, we attempt to summarize best practice of current conservation strategy as well as advantages and disadvantages of applied tools and approaches for listed species or species complexes. The development of conceptual models for the protection of ICS in Europe seems to be necessary; however, currently recommended approaches of conservation strategy of ICS differ according to countries and species. The different approaches to conservation of Astacus astacus and species in the genus Austropotamobius, such as genetic diversity, captive breeding, origin and characteristics of stocking material and establishment, as well as long-term objectives of sanctuary or “ark” sites, were highlighted during the round table discussion.

Published
2011

4. Indigenous crayfish. Habitat and threats

Author

Füreder, L., Edsman, L., Holdich, D., Kozak, P., Machino, Y., Pockl, M., Renai, B., Reynolds, J., Schulz, R., Schulz, H. K., Sint, D., Taugbol, T., Trouilhé, M.-C., Institute of Zoology and Limnology (UNIVERSITY OF INNSBRUCK), University of Innsbruck, National Board of Fisheries (INSTITUTE OF FRESHWATER RESEARCH), Institute of Freshwater Research, Aquatic consultant (AQUATIC CONSULTANT), Aquatic consultant, Research institute of Fish Culture and Hydrobiology (RFC), University of South Bohemia, Higashi-Monzen-cho (HIGASHI-MONZEN-CHO), Sakyo-ku, house 13 (LANDHAUSPLATZ), landhausplatz 1, UNIVERSITY OF FIRENZE Dipt di Biologia Animale e Genetica 'Leo Pardi' (UNIVERSITY OF FIRENZE), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Dept of Zoology (TRINITY COLLEGE), University of Dublin, Institute of Environmental Sciences [Landau], University of Koblenz-Landau, Norwegian Institute for Nature Research (NINA), Génétique et biologie des populations de crustacés (GBPC), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), and Mirebeau, Christelle

Subjects
[SDE.BE] Environmental Sciences/Biodiversity and Ecology, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], [SDV.GEN.GPO] Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Published
2006

5. Plasticity of gene expression according to salinity in the testis of broodstock and F1 black-chinned tilapia,Sarotherodon melanotheron heudelotii

Author

Jean-Christophe Avarre, Rémi Dugué, Marc Legendre, Jacky Cosson, Jean-Dominique Durand, Bruno Guinand, Jacques Panfili, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226, Research institute of Fish Culture and Hydrobiology (RFC), University of South Bohemia, Autonomous intelligent machine (MAIA), Inria Nancy - Grand Est, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Department of Complex Systems, Artificial Intelligence & Robotics (LORIA - AIS), Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Religions et croyances [?-2015] (LARHRA REC [?-2015]), LAboratoire de Recherche Historique Rhône-Alpes - UMR5190 (LARHRA), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Université Pierre Mendès France - Grenoble 2 (UPMF)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Jean Moulin - Lyon 3 (UJML), Université de Lyon-Université de Lyon-Université Pierre Mendès France - Grenoble 2 (UPMF)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Ecologie des systèmes marins côtiers (Ecosym), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Salinity, food.ingredient, Male reproduction, lcsh:Medicine, Zoology, Broodstock, 010603 evolutionary biology, 01 natural sciences, Acclimatization, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, food, 14. Life underwater, Molecular Biology, Sperm motility, 030304 developmental biology, 0303 health sciences, Teleostei, biology, Reproductive success, Ecology, General Neuroscience, lcsh:R, Tilapia, General Medicine, biology.organism_classification, Sperm, Evolutionary Studies, 6. Clean water, Fish, [SDE]Environmental Sciences, Aquaculture, Fisheries and Fish Science, Gene expression, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, General Agricultural and Biological Sciences, Acclimation
Abstract

The black-chinned tilapia Sarotherodon melanotheron heudelotii Rüppell 1852 (Teleostei, Cichlidae) displays remarkable acclimation capacities. When exposed to drastic changes of salinity, which can be the case in its natural habitat, it develops quick physiological responses and keeps reproducing. The present study focused on the physiological impact of salinity on male reproductive capacities, using gene expression as a proxy of acclimation process. Two series of experimental fish were investigated: the first one was composed of fish maintained in freshwater for several generations and newly acclimated to salinities of 35 and 70, whereas the second one consisted of the descendants of the latter born and raised under their native salinity. Expression patterns of 43 candidate genes previously identified from the testes of wild males was investigated in the three salinities and two generations. Twenty of them showed significant expression differences between salinities, and their predicted function revealed that most of them are involved in the osmotic tolerance of sperm cells and/or in the maintenance of sperm motility. A high level of expression variation was evidenced, especially for fish maintained in freshwater. In spite of this, gene expression patterns allowed the differentiation between fish raised in freshwater and those maintained in hypersaline water, in both generations. Altogether, the results presented here suggest that this high variability of expression is likely to ensure the reproductive success of this species under varying salinities.

Published
2014
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