1. Taylor dispersion analysis coupled to ICP-MS for ultrasmall nano-particle size measurement: from drug product to biological media studies
- Author
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Labied, Lucie, Rocchi, Paul, Doussineau, Tristan, Randon, Jérôme, Tillement, Olivier, Lux, François, Hagège, Agnès, Separative Methods - Techniques séparatives, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Formation, élaboration de nanomatériaux et cristaux (FENNEC), Institut Lumière Matière [Villeurbanne] (ILM), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, NH TherAguix SA [Meylan], Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), This work has been supported by a French Government Grant managed by the French National Research Agency (ANR-18-CE17-0025-02)., and ANR-18-CE17-0025,ANALYTAGUIX,Caractérisation analytique de nanoparticules hybrides en vue d'essais cliniques(2018)
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[CHIM]Chemical Sciences ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology - Abstract
The authors would like to thank Mexbrain for providing the cerebrospinal fluid.; International audience; During last decade, special focus has been laid on ultrasmall nanoparticles for nanomedicine and eventual clinical translation. To achieve such translation, a lot of challenges have to be solved. Among them, size determination is a particularly tricky one. In this aim, we have developed a simple hyphenation between Taylor dispersion analysis and ICP-MS. This method was proven to allow the determination of the hydrodynamic radius of metal-containing nanoparticles, even for sizes under 5 nm, with a relative standard deviation below 10% (with a 95% confidence interval) and at low concentrations. Moreover, its specificity provides the opportunity to perform measurements in complex biological media. This was applied to the characterization of an ultrasmall gadolinium-containing nanoparticle, used as theranostic agent in cancer diseases. Hydrodynamic radii measured in urine, cerebrospinal fluid and undiluted serum demonstrated the absence of interaction between the particle and biological compounds such as proteins.
- Published
- 2021
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