1. Recovery of Indicators of Mitochondrial Biogenesis, Oxidative Stress, and Aging With (-)-Epicatechin in Senile Mice.
- Author
-
Moreno-Ulloa, Aldo, Nogueira, Leonardo, Rodriguez, Alonso, Barboza, Jonathan, Hogan, Michael C., Ceballos, Guillermo, Villarreal, Francisco, and Ramirez-Sanchez, Israel
- Subjects
MITOCHONDRIA formation ,OXIDATIVE stress ,EPICATECHIN ,PHYSIOLOGICAL aspects of aging ,GLUTATHIONE ,BIOMARKERS ,HEART metabolism ,PROTEIN metabolism ,BRAIN metabolism ,AGE distribution ,AGING ,ANIMAL experimentation ,BRAIN ,FLAVONOIDS ,GLYCOSIDASES ,KIDNEYS ,MICE ,MYOCARDIUM ,OXIDOREDUCTASES ,RESEARCH funding ,TRANSFERASES ,SKELETAL muscle - Abstract
There is evidence implicating oxidative stress (OS) as the cause of the deleterious effects of aging. In this study, we evaluated the capacity of the flavanol (-)-epicatechin (Epi) to reduce aging-induced OS and restore mitochondrial biogenesis, as well as, structural and functional endpoints in aged mice. Senile (S; 26-month-old) C57BL/6 male mice were randomly assigned to receive either water (vehicle) or 1mg/kg of Epi via oral gavage (twice daily) for 15 days. Young (Y; 6-month-old) mice were used as controls. In S brain, kidney, heart, and skeletal muscle (compared with Y animals) an increase in OS was observed as evidenced by increased protein-free carbonyls and decreased reduced glutathione levels as well as sirtuin 3, superoxide dismutase 2, catalase, thioredoxin and glutathione peroxidase protein levels. Well-recognized factors (eg, sirtuin 1) that regulate mitochondrial biogenesis and mitochondrial structure- and/or function-related endpoints (eg, mitofilin and citrate synthase) protein levels were also reduced in S organs. In contrast, the aging biomarker senescence-associated β-galactosidase was increased in S compared with Y animals, and Epi administration reduced levels towards those observed in Y animals. Altogether, these data suggest that Epi is capable of shifting the biology of S mice towards that of Y animals. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF