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In vitro protein and starch digestions in five sorghum-barley mixtures were investigated, revealing monophasic, and rapid-slow and slow-rapid multiphasic digestograms. The digestograms were adequately (r2 > 0.93, p ≤ 0.001) modelled by the Sopade Objective Procedure (best for the starch digestograms), and multiterm exponential (best for the protein digestograms) and non-exponential equations. The mixtures varied in physicochemical and digestion properties, showing low sorghum digestibilities. The sorghum delayed pasting and increased anthocyanins to possibly inhibit the digestions. Towards better predicting in vitro protein digestibility, a hydrolysis index relative to casein (HI PROTEIN) is presented and correlated (r2 > 0.98, p ≤ 0.001) with the hydrolysis index for starch digestion (HI STARCH) relative to white wheat bread. Using five published in vitro - in vivo protein digestibility data, a general equation (INVIVO PROTEIN = 0.29 HI PROTEIN + 61.89; r2 = 0.960, p = 0.003) was obtained to, with the existing glycaemic-index-HI STARCH equation, better understand food digestions. [Display omitted] • Five sorghum-barley mixtures, examples of beneficial multigrain systems, were studied. • Digestion and non-digestion properties revealed blended characteristics of the grains. • The mixtures exhibited mono- and multi-phasic in vitro protein and starch digestograms. • CaseIn-referenced hydrolysis index of protein digestion is defined for the first time. • The mixtures' digestion and non-digestion properties differently correlated. [ABSTRACT FROM AUTHOR]

The effects of formulating broiler diets that contain sources of either rapidly or slowly digestible protein and 2 different dietary fiber sources on growth performance were studied in broilers chickens from 0 to 36 d of age. A total of 1,920 one-day-old, male Ross 708 broiler chickens were randomly allocated and housed in 48 floor pens (40 birds/pen) to one of 4 dietary treatments. Birds were allotted according to a completely randomized block design using a factorial arrangement of treatments with 2 protein digestion rates (rapidly or slowly) and 2 dietary fiber sources [3% oat hulls (OH) or 3% sugar beet pulp (SBP)] from 0 to 36 d of age. All diets were formulated to be isocaloric and isonitrogenous. The pen was the experimental unit for all variables studied (12 replicates/treatment). Data were analyzed using the MIXED procedure of SAS, and the model included the main effects of the protein digestion rate, dietary fiber source, and their interaction. There were 3 experimental feeding phases; starter (from day 0–14), grower (from day 14–28), and finisher (from day 28–36). Results indicated that broilers fed diets containing sources that supplied more rapidly digestible protein had 4% greater (P

Body protein losses in lactating sows have a negative impact on sow and litter performance. Improving dietary amino acid utilization may limit protein mobilization. The effects of dietary protein kinetics on sow body condition loss, blood plasma metabolites, and plasma insulin-like growth factor-1 (IGF-1), and also on litter gain during lactation, were investigated in this study. In total, 57 multiparous sows were fed one of three lactation diets with the same crude protein level: low level of slow protein diet (LSP) (8% slowly degradable protein of total protein), medium level of slow protein diet (MSP) (12% slowly degradable protein of total protein), or high level of slow protein diet (HSP) (16% slowly degradable protein of total protein) in a complete block design. Our results showed that HSP sows lost the least body weight compared to MSP and LSP sows (11.9 vs. 17.3 and 13.5 kg, respectively; p = 0.01), less body protein than MSP sows (1.0 vs. 2.1 kg; p = 0.01), and tended to lose less loin muscle thickness than LSP sows (1.7 vs. 4.9 mm; p = 0.09) between Day 2 to Day 21 post-farrowing. LSP sows had greatest plasma urea level on Day 6 than MSP and HSP sows (4.9 vs. 3.6 and 3.1 mmol/L, respectively; p < 0.01) and on Day 13 (5.6 vs. 4.1 and 3.7 mmol/L, respectively; p < 0.01). HSP sows had the lowest plasma urea level at Day 20 compared to LSP and MSP sows (4.0 vs. 5.5 and 4.9 mmol/L, respectively; p < 0.01). The average plasma urea level of Days 6, 13, and 20 post-farrowing was negatively correlated with slow protein intake (r = −0.49, p < 0.01). Litter gain, milk composition, and nitrogen output to the environment did not differ significantly among the treatment groups. Therefore, the dietary protein kinetics affected mobilization of maternal reserves in multiparous sows during lactation, with a high fraction of slow protein-sparing protein mobilization.