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Objective: To reveal whether gut microbiota and their metabolites are correlated with oocyte quality decline caused by circadian rhythm disruption, and to search possible approaches for improving oocyte quality. Design: A mouse model exposed to continuous light was established. The oocyte quality, embryonic development, microbial metabolites and gut microbiota were analyzed. Intragastric administration of microbial metabolites was conducted to confirm the relationship between gut microbiota and oocyte quality and embryonic development. Results: Firstly, we found that oocyte quality and embryonic development decreased in mice exposed to continuous light. Through metabolomics profiling and 16S rDNA‐seq, we found that the intestinal absorption capacity of vitamin D was decreased due to significant decrease of bile acids such as lithocholic acid (LCA), which was significantly associated with increased abundance of Turicibacter. Subsequently, the concentrations of anti‐Mullerian hormone (AMH) hormone in blood and melatonin in follicular fluid were reduced, which is the main reason for the decline of oocyte quality and early embryonic development, and this was rescued by injection of vitamin D3 (VD3). Secondly, melatonin rescued oocyte quality and embryonic development by increasing the concentration of lithocholic acid and reducing the concentration of oxidative stress metabolites in the intestine. Thirdly, we found six metabolites that could rescue oocyte quality and early embryonic development, among which LCA of 30 mg/kg and NorDCA of 15 mg/kg had the best rescue effect. Conclusion: These findings confirm the link between ovarian function and gut microbiota regulation by microbial metabolites and have potential value for improving ovary function. [ABSTRACT FROM AUTHOR]

Abstract: Nearest neighbor (NN) classification assumes locally constant class conditional probabilities, and suffers from bias in high dimensions with a small sample set. In this paper, we propose a novel cam weighted distance to ameliorate the curse of dimensionality. Different from the existing neighborhood-based methods which only analyze a small space emanating from the query sample, the proposed nearest neighbor classification using the cam weighted distance (CamNN) optimizes the distance measure based on the analysis of inter-prototype relationship. Our motivation comes from the observation that the prototypes are not isolated. Prototypes with different surroundings should have different effects in the classification. The proposed cam weighted distance is orientation and scale adaptive to take advantage of the relevant information of inter-prototype relationship, so that a better classification performance can be achieved. Experiments show that CamNN significantly outperforms one nearest neighbor classification (1-NN) and -nearest neighbor classification (-NN) in most benchmarks, while its computational complexity is comparable with that of 1-NN classification. [Copyright &y& Elsevier]

As a member of Ubiquitin-specific protease subfamily, ubiquitin specific protease 7 (USP7) has been reported to participate in a variety of cellular processes, including cell cycle, apoptosis, DNA damage response, and epigenetic modification. However, its function in preimplantation embryos is still obscure. To investigate the functions of USP7 during preimplantation embryo development, we used siRNA to degrade endogenous USP7 messenger RNA. We found that USP7 knockdown significantly decreased the development rate of mouse early embryos. Moreover, depletion of USP7 induced the accumulation of the DNA lesions and apoptotic blastomeres in early embryos. In addition, USP7 knockdown caused an abnormal H3K27me3 modification in 2-cell embryos. Overall, our results indicate that USP7 maintains genome stability perhaps via regulating H3K27me3 and DNA damage, consequently controlling the embryo quality. • Depletion of USP7 compromises the early embryo development. • USP7 knockdown increases DNA damage and apoptosis in mouse early embryos. • USP7 knockdown causes an abnormal H3K27me3 modification in mouse early embryos. [ABSTRACT FROM AUTHOR]