1. A novel magnetic solid-phase extraction method for detection of 14 heterocyclic aromatic amines by UPLC-MS/MS in meat products
- Author
-
Shan Shihui, Xianbing Xu, Xiao-lu Guo, Changling Sun, Yun-Jiao Ma, Han Zheng, Jiang-Ning Hu, and Lei Qin
- Subjects
Indoles ,Polymers ,01 natural sciences ,High-performance liquid chromatography ,Analytical Chemistry ,Matrix (chemical analysis) ,0404 agricultural biotechnology ,Heterocyclic Compounds ,Tandem Mass Spectrometry ,Quinoxalines ,Spectroscopy, Fourier Transform Infrared ,Animals ,Solid phase extraction ,Amines ,Chromatography ,Chemistry ,Magnetic Phenomena ,Solid Phase Extraction ,010401 analytical chemistry ,Extraction (chemistry) ,Imidazoles ,04 agricultural and veterinary sciences ,General Medicine ,040401 food science ,0104 chemical sciences ,Meat Products ,Microscopy, Electron, Scanning ,Pork Meat ,Quinolines ,Nanoparticles ,Uplc ms ms ,Heterocyclic Aromatic Amines ,Food Analysis ,Carbolines ,Chromatography, Liquid ,Food Science - Abstract
The current study developed a cheap and effective method for the simultaneous extraction of 14 heterocyclic aromatic amines (HAAs) in food matrix. Core-shell Fe3O4@PDA nanoparticles were constructed and acted as the magnetic solid-phase extraction adsorbent to separate and purify HAAs from meat products for the first time. Then, UPLC-MS/MS technique was employed to identify and quantify the HAAs easily. Fe3O4@PDA nanoparticles were synthesized and characterized successfully. Totally 14 HAAs were completely separated in 19.99 min with good regression coefficients. LODs and LOQs were in the range of 0.013–0.247 ng/g and 0.056–0.803 ng/g, respectively. The intra-day precisions and inter-day precisions were below 9%. Except for IQ[4,5-b], Phe-p-1, PhIP, other 11 types of HAAs (DMIP, 1,5,6-TMIP, IQ, IQx, MeIQ, MeIQx, 7,8-DiMeIQx, AαC, MeAαC, Harman, Norharman) could acquire relatively high recoveries (71.06%–108.49%). The proposed method was successfully devoted to the evaluation of HAAs levels in 8 commercial meat products to verify the adaptability.
- Published
- 2021
- Full Text
- View/download PDF