32 results on '"Yim, Nam-Hui"'
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2. Secondary metabolites and transcriptomic analysis of novel pulcherrimin producer Metschnikowia persimmonesis KIOM G15050: A potent and safe food biocontrol agent
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Rahmat, Endang, Yu, Jae Sik, Lee, Bum Soo, Lee, Jiyoung, Ban, Yeongjun, Yim, Nam-Hui, Park, Jeong Hwan, Kang, Chang Ho, Kim, Ki Hyun, and Kang, Youngmin
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- 2024
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3. Hoveniae Semen Seu Fructus water extract inhibits influenza A virus infection
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Cho, Won-Kyung, Cha, Min-Ho, Yim, Nam-Hui, Choi, Hee-Jeong, and Ma, Jin Yeul
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- 2024
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4. The antiviral activity of Thuja orientalis folium against Influenza A virus
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Lee, Myong-Min, Cho, Won-Kyung, Cha, Min Ho, Yim, Nam-Hui, Yang, Hye Jin, and Ma, Jin Yeul
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- 2023
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5. GC–MS and LC-TOF–MS profiles, toxicity, and macrophage-dependent in vitro anti-osteoporosis activity of Prunus africana (Hook f.) Kalkman Bark
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Komakech, Richard, Shim, Ki-Shuk, Yim, Nam-Hui, Song, Jun Ho, Yang, Sungyu, Choi, Goya, Lee, Jun, Kim, Yong-goo, Omujal, Francis, Okello, Denis, Agwaya, Moses Solomon, Kyeyune, Grace Nambatya, Kan, Hyemin, Hwang, Kyu-Seok, Matsabisa, Motlalepula Gilbert, and Kang, Youngmin
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- 2022
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6. Anti-Fibrosis Effect of Panax ginseng and Inula japonica Formula in Human Pulmonary Fibroblasts.
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Jung, YeonGyun, Yim, Nam-Hui, Lee, Sang Myung, Cho, Won-Kyung, Cha, Min Ho, and Ma, Jin Yeul
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Panax ginseng Meyer and Inula japonica Thunb. are well established in traditional medicine and are known for their therapeutic properties in managing a range of ailments such as diabetes, asthma, and cancer. Although P. ginseng and I. japonica can alleviate pulmonary fibrosis (PF), the anti-fibrosis effect on PF by the combination of two herbal medicines remains unexplored. Therefore, this study explores this combined effect. In conditions that were not cytotoxic, MRC-5 cells underwent treatment using the formula combining P. ginseng and I. japonica (ISE081), followed by stimulation with transforming growth factor (TGF)-β1, to explore the fibroblast-to-myofibroblast transition (FMT). After harvesting the cells, mRNA levels and protein expressions associated with inflammation and FMT-related markers were determined to evaluate the antiinflammation activities and antifibrosis effect of ISE081. Additionally, the anti-migratory effects of ISE081 were validated through a wound-healing assay. ISE081 remarkably reduced the mRNA levels of interleukin (IL)-6, IL-8, α-smooth muscle actin (SMA), and TGF-β1 in MRC-5 cells and suppressed the α-SMA and fibronectin expressions, respectively. Furthermore, ISE081 inhibited Smad2/3 phosphorylation and wound migration of MRC-5 cells. Under the same conditions, comparing those of ISE081, P. ginseng did not affect the expression of α-SMA, fibronectin, and Smad2/3 phosphorylation, whereas I. japonica significantly inhibited them but with cytotoxicity. The results indicate that the synergistic application of P. ginseng and I. japonica enhances the anti-fibrotic properties in pulmonary fibroblasts and concurrently diminishes toxicity. Therefore, ISE081 has the potential as a prevention and treatment herbal medicine for PF. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Water extract of Galla Rhois with steaming process enhances apoptotic cell death in human colon cancer cells
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Yim, Nam-Hui, Gu, Min Jung, Hwang, Youn-Hwan, Cho, Won-Kyung, and Ma, Jin Yeul
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- 2016
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8. Protective Effects of Spatholobi Caulis Extract on Neuronal Damage and Focal Ischemic Stroke/Reperfusion Injury
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Park, Hee Ra, Lee, Heeeun, Lee, Jung-Jin, Yim, Nam-Hui, Gu, Min-Jung, and Ma, Jin Yeul
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- 2018
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9. Simultaneous determination of nine bioactive compounds in Yijin-tang via high-performance liquid chromatography and liquid chromatography-electrospray ionization-mass spectrometry
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Yang, Hye Jin, Yim, Nam-Hui, Lee, Kwang Jin, Gu, Min Jung, Lee, Bohyoung, Hwang, Youn-Hwan, and Ma, Jin Yeul
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- 2016
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10. Screening of aqueous extracts of medicinal herbs for antimicrobial activity against oral bacteria
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Yim, Nam-Hui, Jung, Young Pil, Cho, Won-Kyung, Kim, Taesoo, Kim, Aeyung, Im, Minju, and Ma, Jin Yeul
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- 2013
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11. Decursin and decursinol angelate from Angelica gigas Nakai induce apoptosis via induction of TRAIL expression on cervical cancer cells
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Yim, Nam-Hui, Lee, Ju Hye, Cho, Won-Kyung, Yang, Min Chul, Kwak, Dong Hoon, and Ma, Jin Yeul
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- 2011
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12. Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model.
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Yim, Nam-Hui, Park, Eunhee, Cho, Won-Kyung, Kim, Yeoun-Hee, and Ma, Jin Yeul
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DRY eye syndromes , *TREATMENT effectiveness , *TOPICAL drug administration , *LABORATORY mice , *ORAL drug administration , *MEIBOMIAN glands - Abstract
We determined the effects of two extracts from Acer palmatum Thumb. leaves (hot water extract KIOM-2015EW and 25% ethanol extract KIOM-2015EE) in a benzalkonium chloride (BAC)-induced dry eye mouse model. Dry eye was induced by 0.2% BAC for 2 weeks, followed by treatment three times (eye drop) or once (oral administration) daily with KIOM-2015E for 2 weeks. Treatment with both KIOM-2015EE and KIOM-2015EW resulted in a marked increase in tear volume production for the 4 days of treatment. The Lissamine Green staining score, TUNEL-positive cells, and inflammatory index were significantly decreased after 2 weeks. Topical KIOM-2015EE administration exhibited a greater improvement in decreasing the ocular surface staining scores, inflammation, dead cells, and increasing tear production in a dose-dependent manner compared with the other groups. Furthermore, KIOM-2015E significantly reduced the phosphorylation of NF-κB, which was activated in the BAC-treated cornea. Topical administration was much more effective than oral administration for KIOM-2015E and KIOM-2015EE was more effective than KIOM-2015EW. Application of KIOM-2015E resulted in clinical improvement, inhibited the inflammatory response, and alleviated signs of dry eye. These results indicate that KIOM-2015E has potential as a therapeutic agent for the clinical treatment of dry eye. [ABSTRACT FROM AUTHOR]
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- 2022
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13. Induction of apoptotic cell death by betulin in multidrug-resistant human renal carcinoma cells
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YIM, NAM-HUI, JUNG, YOUNG PIL, KIM, AEYUNG, KIM, TAESOO, and MA, JIN YEUL
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- 2015
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14. Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants
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Chung, Kyung-Sook, Yim, Nam-Hui, Lee, Seung-Hee, Choi, Shin-Jung, Hur, Kyung-Sun, Hoe, Kwang-Lae, Kim, Dong-Uk, Goehle, Sondra, Kim, Hyung-Bae, Song, Kyung-Bin, Yoo, Hyang-Sook, Bae, Ki-Hwan, Simon, Julian, and Won, Misun
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- 2008
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15. Broccoli Leaves Attenuate Influenza A Virus Infection by Interfering With Hemagglutinin and Inhibiting Viral Attachment.
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Cho, Won-Kyung, Yim, Nam-Hui, Lee, Myong-Min, Han, Chang-Hoon, and Ma, Jin Yeul
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VIRUS diseases ,INFLUENZA A virus ,INFLUENZA viruses ,INFLUENZA ,BROCCOLI ,GREEN fluorescent protein - Abstract
Broccoli (Brassica oleracea L. var. Italica) leaves are a byproduct of broccoli and could be used as a food source. The study aimed to evaluate the effect of broccoli leaves on influenza A virus (IAV) infection. We investigated the effect of ethanol extract of Broccoli leaves (EBL) on IAV infection using green fluorescent protein (GFP)–tagged Influenza A/PR/8/34 virus (PR8-GFP IAV). When EBL and PR8-GFP IAV were cotreated to RAW 264.7 cells, the fluorescence microscopy and fluorescence-activated cell sorting (FACS) analysis showed that EBL significantly reduced the levels of GFP expression by influenza viral infection dose-dependently. Immunofluorescence (IF) analysis confirmed that EBL decreased the expression of IAV proteins. EBL exhibited a strong inhibitory effect of IAV binding on the cells and moderate virucidal impact. Consistently, EBL potently suppressed the hemagglutination by IAV infection. These results indicate that EBL prevents IAV attachment via the inhibition of HA upon viral infection. Finally, EBL as an HA inhibitor of IAV could be used as the natural antiviral source to protect against influenza viral infection. [ABSTRACT FROM AUTHOR]
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- 2022
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16. Root Extract of a Micropropagated Prunus africana Medicinal Plant Induced Apoptosis in Human Prostate Cancer Cells (PC-3) via Caspase-3 Activation.
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Komakech, Richard, Yim, Nam-Hui, Shim, Ki-Shuk, Jung, Haiyoung, Byun, Jae-Eun, Lee, Jun, Okello, Denis, Matsabisa, Motlalepula Gilbert, Erhabor, Joseph O., Oyenihi, Omolola, Omujal, Francis, Agwaya, Moses, Kim, Yong-goo, Park, Jeong Hwan, and Kang, Youngmin
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IN vitro studies , *STATISTICS , *MEDICINAL plants , *STAINS & staining (Microscopy) , *HIGH performance liquid chromatography , *ANALYSIS of variance , *WESTERN immunoblotting , *DOXORUBICIN , *APOPTOSIS , *PLANT roots , *CELL survival , *GAS chromatography , *BARK , *DOSE-effect relationship in pharmacology , *MASS spectrometry , *CELL proliferation , *DESCRIPTIVE statistics , *PLANT extracts , *CELL lines , *DATA analysis , *PROSTATE tumors , *CASPASES , *PHARMACODYNAMICS - Abstract
Prostate cancer is one of the major causes of cancer-related deaths among men globally. Medicinal plants have been explored as alternative treatment options. Herein, we assessed the in vitro cytotoxic effects of 70% ethanolic root extracts of six-month-old micropropagated Prunus africana (PIR) on PC-3 prostate cancer cells as an alternative to the traditionally used P. africana stem-bark extract (PWS) treatment. In vitro assays on PC-3 cells included annexin-V and propidium iodide staining, DAPI staining, and caspase-3 activity analysis through western blotting. PC-3 cells were exposed to PWS and PIR at different concentrations, and dose-dependent antiprostate cancer effects were observed. PC-3 cell viability was determined using CCK-8 assay, which yielded IC50 values of 52.30 and 82.40 μg/mL for PWS and PIR, respectively. Annexin-V and PI staining showed dose-dependent apoptosis of PC-3 cells. Significant (p < 0.001) percent of DAPI-stained apoptotic PC-3 cells were observed in PWS, PIR, and doxorubicin treatment compared with the negative control. PWS treatment substantially elevated cleaved caspase-3 levels in PC-3 cells compared with the PIR treatment. These results provide evidence for the antiprostate cancer potential of PIR and sets a basis for further research to enhance future utilization of roots of young micropropagated P. africana for prostate cancer treatment as an alternative to stem bark. Moreover, micropropagation approach may help provide the required raw materials and hence reduce the demand for P. africana from endangered wild population. [ABSTRACT FROM AUTHOR]
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- 2022
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17. In Vitro Antiosteoporosis Activity and Hepatotoxicity Evaluation in Zebrafish Larvae of Bark Extracts of Prunus jamasakura Medicinal Plant.
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Komakech, Richard, Shim, Ki-Shuk, Yim, Nam-Hui, Song, Jun Ho, Yang, Sun Kyu, Choi, Goya, Lee, Jun, Kim, Yong-goo, Omujal, Francis, Agwaya, Moses, Nambatya, Grace Kyeyune, Kan, Hyemin, Hwang, Kyu-Seok, Motlalepula, Gilbert Matsabisa, and Kang, Youngmin
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APOPTOSIS ,DIMETHYL sulfoxide ,FISHES ,HEPATOTOXICOLOGY ,MEDICINAL plants ,MEMBRANE proteins ,NITRIC oxide ,TRADITIONAL medicine ,DRUG development ,LIPOPOLYSACCHARIDES ,CELL survival ,DESCRIPTIVE statistics ,IN vitro studies - Abstract
Osteoporosis is one of the main health problems in the world today characterized by low bone mass and deterioration in bone microarchitecture. In recent years, the use of natural products approach to treat it has been in the increase. In this study, in vitro antiosteoporosis activity and hepatotoxicity of P. jamasakura bark extracts were evaluated. Methods. Mouse bone marrow macrophage (BMM) cells were incubated with tartrate-resistant acid phosphate (TRAP) buffers and p-nitrophenyl phosphate and cultured with different P. jamasakura bark extracts at concentrations of 0, 6.25, 12.5, 25, and 50 μg/ml in the presence of the receptor activator of nuclear factor kappa-Β ligand (RANKL) for 6 days. The osteoclast TRAP activity and cell viability were measured. Nitric oxide (NO) assay was conducted using murine macrophage-like RAW 264.7 cells treated with P. jamasakura ethanolic and methanolic bark extracts at concentrations of 0, 6.25, 12.5, 25, 50, 100, and 200 μg/ml. For hepatotoxicity assessment, zebrafish larvae were exposed to P. jamasakura bark extracts, 0.05% dimethyl sulfoxide as a negative control, and 5 μM tamoxifen as a positive control. The surviving larvae were anesthetized and assessed for hepatocyte apoptosis. Results. TRAP activity was significantly inhibited (p < 0.001) at all concentrations of P. jamasakura extracts compared to the control treatment. At 50 μg/ml, both ethanolic and methanolic extracts of P. jamasakura exhibited significant (p < 0.01) BMM cell viability compared to the control treatment. P. jamasakura ethanolic and methanolic extracts had significant inhibitory (p < 0.01) effects on lipopolysaccharide (LPS)-induced NO production at 200 μg/ml and exhibited significant (p < 0.01) and (p < 0.05) stimulative effects, respectively, on RAW 264.7 cell viability. No overt hepatotoxicity was observed in the liver of zebrafish larvae in any of the treatments. Conclusion. The TRAP activity of P. jamasakura bark gives a foundation for further studies to enhance future development of antiosteoporosis drug. [ABSTRACT FROM AUTHOR]
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- 2020
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18. Hoveniae Semen Seu Fructus Ethanol Extract Exhibits Anti-Inflammatory Activity via MAPK, AP-1, and STAT Signaling Pathways in LPS-Stimulated RAW 264.7 and Mouse Peritoneal Macrophages.
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Jeong, Yun Hee, Oh, You-Chang, Cho, Won-Kyung, Yim, Nam-Hui, and Ma, Jin Yeul
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PERITONEAL macrophages ,SEMEN ,RAW foods ,THERAPEUTICS ,WESTERN immunoblotting ,LIPOPOLYSACCHARIDES ,FRUIT seeds ,MACROPHAGE inflammatory proteins - Abstract
Hoveniae semen seu fructus (HSF, fruit and seed of Hovenia dulcis Thunb) is an important traditional herbal medicine and food supplement in East Asia for the treatment of liver diseases, alcohol poisoning, obesity, allergy, and cancer. HSF has also been reported to have anti-inflammatory activity, but the cellular mechanism of action is not fully understood. We assessed the anti-inflammatory properties of an HSF ethanol (HSFE) extract and explored its precise mechanism. The ability of HSFE to suppress inflammatory responses was investigated in a murine macrophage cell line, RAW 264.7, and mouse primary macrophages. Secretions of NO, proinflammatory cytokines, inflammatory factors, and related proteins were measured using the Griess assay, ELISA, Western blot analysis, and real-time PCR, respectively. In addition, the main components of HSFE were analyzed by HPLC, and their anti-inflammatory activity was confirmed. Our results showed that pretreatment of HSFE markedly reduced the expression of NO and iNOS without causing cytotoxicity and significantly attenuated secretion of proinflammatory cytokines, including TNF-α, IL-6, and IL-1β. In addition, HSFE strongly suppressed phosphorylation of MAPK and decreased the activation of AP-1, JAK2/STAT, and NF-κB in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. Furthermore, HSFE strongly suppressed the inflammatory cytokine levels in mouse peritoneal macrophages. Also, as a result of HPLC analysis, three main components, ampelopsin, taxifolin, and myricetin, were identified in the HSFE extract, and each compound effectively inhibited the secretion of inflammatory mediators induced by LPS. These findings show that HSFE exerts anti-inflammatory effects by suppressing the activation of MAPK, AP-1, JAK2/STAT, and NF-κB signaling pathways in LPS-stimulated macrophages. In addition, the anti-inflammatory efficacy of HSFE appears to be closely related to the action of the three main components. Therefore, HSFE appears to be a promising candidate for the treatment of inflammatory diseases. [ABSTRACT FROM AUTHOR]
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- 2019
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19. Enhancement of neuroprotective activity of Sagunja-tang by fermentation with lactobacillus strains.
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Yim, Nam-Hui, Gu, Min Jung, Park, Hee Ra, Hwang, Youn-Hwan, and Ma, Jin Yeul
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REACTIVE oxygen species ,BIOLOGICAL transport ,CELL death ,CELL lines ,ETOPOSIDE ,FERMENTATION ,GLYCOSIDES ,HERBAL medicine ,HIGH performance liquid chromatography ,HYDROGEN peroxide ,LACTOBACILLUS ,LIQUID chromatography ,MASS spectrometry ,CHINESE medicine ,NERVOUS system ,NEUROBLASTOMA ,TERPENES ,PHYTOCHEMICALS ,OXIDATIVE stress - Abstract
Background: Sagunja-tang (SGT) is widely used in traditional herbal medicine to treat immune system and gastrointestinal disorders and reportedly has protective effects against inflammation, cancer, and osteoporosis. In this study, we fermented SGT with different Latobacillus strains and investigated the change in phytochemical compositions in SGT and enhancement of it neuroprotective effects in SH-SY5Y human neuroblastoma. Methods: Marker components, including ginsenoside Rg
1 , glycyrrhizin, liquiritin, liquiritigenin, atractylenolide I, atractylenolide II, atractylenolide III, and pachymic acid, in SGT, were qualitatively and quantitatively analyzed using high-performance liquid chromatography–diode array detection and liquid chromatography–mass spectrometry. SGT was fermented with eight different Lactobacillus strains to yield eight fermented SGTs (FSGTs). The conversion efficiencies of SGT marker components were determined in each FSGT. To detect the protective effect of SGT and FSGT, reactive oxygen species (ROS) assay and mitochondrial membrane potentials (MMPs) assay were performed in SH-SY5Y cells. Results: Compared with the other FSGTs, SGT166, i.e., SGT fermented with L. plantarum 166, had high conversion efficiency, as indicated by increased amounts of glycyrrhizin, liquiritigenin, and atractylenolides I–III. In SH-SY5Y cells, protection against cell death induced by H2 O2 and etoposide was high using SGT166 and very low using SGT. Furthermore, ROS production and mitochondrial membrane potential disruption in SH-SY5Y cells were markedly suppressed by SGT166 treatment, which demonstrated that inhibition of ROS generation may be one of the neuroprotective mechanisms of SGT166. Conclusions: This study demonstrated that fermentation of SGT with L. plantarum 166 enhanced suppression of oxidative stress and MMP loss. This enhanced neuroprotective effect was thought to be caused by the conversion of SGT phytochemicals by fermentation. SGT166 shows potential for treating neurological damage-related diseases. [ABSTRACT FROM AUTHOR]- Published
- 2018
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20. Anti-Inflammatory and Anti-Apoptotic Effects of Acer Palmatum Thumb. Extract, KIOM-2015EW, in a Hyperosmolar-Stress-Induced In Vitro Dry Eye Model.
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Kim, Yeoun-Hee, Oh, Tae Woo, Park, Eunhee, Yim, Nam-Hui, Park, Kang Il, Cho, Won Kyung, and Ma, Jin Yeul
- Abstract
The aim of this study was to assess the anti-inflammatory and anti-apoptotic effects of KIOM-2015EW, the hot-water extract of maple leaves in hyperosmolar stress (HOS)-induced human corneal epithelial cells (HCECs). HCECs were exposed to hyperosmolar medium and exposed to KIOM-2015EW with or without the hyperosmolar media. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production and apoptosis were observed, and the activation of mitogen-activated protein kinases (MAPKs) including extracellular signal regulated kinase (ERK), p38 and c-JUN N-terminal kinase (JNK) signaling and nuclear factor (NF)-κB was confirmed. Compared to isomolar medium, the induction of cell cytotoxicity significantly increased in HCECs exposed to hyperosmolar medium in a time-dependent manner. KIOM-2015EW-treatment significantly reduced the mRNA and protein expression of pro-inflammatory mediators and apoptosis. KIOM-2015EW-treatment inhibited HOS-induced MAPK signaling activation. Additionally, the HOS-induced increase in NF-κB phosphorylation was attenuated by KIOM-2015EW. The results demonstrated that KIOM-2015EW protects the ocular surface by suppressing inflammation in dry eye disease, and suggest that KIOM-2015EW may be used to treat several ocular surface diseases where inflammation plays a key role. [ABSTRACT FROM AUTHOR]
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- 2018
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21. A platycoside-rich fraction from the root of Platycodon grandiflorum enhances cell death in A549 human lung carcinoma cells via mainly AMPK/mTOR/AKT signal-mediated autophagy induction.
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Yim, Nam–Hui, Hwang, Youn–Hwan, Liang, Chun, and Ma, Jin Yeul
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CANCER prevention , *PROTEIN metabolism , *LUNG tumors , *AUTOPHAGY , *ALTERNATIVE medicine , *ANTINEOPLASTIC agents , *BIOLOGICAL assay , *BIOLOGICAL models , *CELL death , *CELLULAR signal transduction , *CYTOPLASM , *DOSE-effect relationship in pharmacology , *HIGH performance liquid chromatography , *MEDICINAL plants , *MICROSCOPY , *PHOSPHORYLATION , *PROTEIN kinases , *PLANT roots , *WESTERN immunoblotting , *PLANT extracts , *STATISTICAL significance , *IN vitro studies , *PHARMACODYNAMICS , *PREVENTION - Abstract
Ethnopharmacological relevance The root of Platycodon grandiflorum (PG), commonly known as Kilkyong in Korea, Jiegeng in China, and Kikyo in Japan, has been extensively used as a traditional anti-inflammatory medicine in Asia for the treatment of respiratory conditions, such as bronchitis, asthma, and tonsillitis. Platycosides isolated from PG are especially well-known for their anti-cancer effects. Aim of the study We investigated the involvement of autophagic cell death and other potential molecular mechanisms induced by the platycoside-containing butanol fraction of PG (PGB) in human lung carcinoma cells. Materials and methods PGB-induced growth inhibition and cell death were measured using a 5-diphenyl-tetrazolium bromide (MTT) assay. The effects of PGB on autophagy were determined by observing microtubule-associated protein 1 light chain 3 (LC3) redistribution with confocal microscopy. The PGB-mediated regulation of autophagy-associated proteins was investigated using Western blotting analysis. Furthermore, the anti-cancer mechanism of PGB was confirmed using chemical inhibitors. A high-performance liquid chromatography (HPLC)-DAD system was used to analyze the platycosides in PGB. Results In A549 cells, PGB induced significant autophagic cell death. Specifically, PGB upregulated LC3-II in a time- and dose-dependent manner, and it redistributed LC3 via autophagosome formation in the cytoplasm. PGB treatment increased the phosphorylation of AMP-activated protein kinase (AMPK) and subsequently suppressed the AKT/mammalian target of the rapamycin (mTOR) pathway. Furthermore, PGB inhibited cell proliferation by regulating the mitogen-activated protein kinase (MAPK) pathways. In this study, six types of platycosides were identified in the PGB using HPLC. Conclusions PGB efficiently induced cancer cell death via autophagy and the modulation of the AMPK/mTOR/AKT and MAPK signaling pathways in A549 cells. Therefore, PGB may be an efficacious herbal anti-cancer therapy. [ABSTRACT FROM AUTHOR]
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- 2016
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22. Alpinia officinarum Stimulates Osteoblast Mineralization and Inhibits Osteoclast Differentiation.
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Shim, Ki-Shuk, Lee, Chung-Jo, Yim, Nam-Hui, Gu, Min Jung, and Ma, Jin Yeul
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BONE metabolism ,ANIMAL experimentation ,BONE resorption ,HIGH performance liquid chromatography ,MICE ,OSTEOPOROSIS ,POLYMERASE chain reaction ,RESEARCH funding ,T-test (Statistics) ,PLANT extracts ,OSTEOBLASTS ,IN vitro studies - Abstract
Alpinia officinarum rhizome has been used as a traditional herbal remedy to treat inflammatory and internal diseases. Based on the previously observed inhibitory effect of A. officinarum rhizome in an arthritis model, we evaluated whether a water extract of A. officinarum rhizome (WEAO) would enhance in vitro osteoblast mineralization using calvarial osteoblast precursor cells or would inhibit in vitro osteoclast differentiation and bone resorption using bone marrow derived macrophages. In osteoblasts, WEAO enhanced the mRNA levels of transcription factor ( runt-related transcription factor 2, smad1, smad5, and junB) and marker ( bone morphogenetic protein-2, collagen type 1alpha1, and osteocalcin) genes related to osteoblast mineralization, consistent with increased alizarin red S staining intensity. WEAO markedly inhibited osteoclast differentiation by suppressing the receptor activator for nuclear factor-B ligand-induced downregulation of inhibitor of DNA binding 2 and V-maf musculoaponeurotic fibrosarcoma oncogene homolog B and the phosphorylation of c-Jun N-terminal kinase, p38, nuclear factor-B, c-Src, and Bruton's tyrosine kinase to induce nuclear factor of activated T cells cytoplasmic 1 expression. WEAO also suppressed the resorbing activity of mature osteoclasts by altering actin ring formation. Therefore, the results of this study demonstrate that WEAO stimulates osteoblast mineralization and inhibits osteoclast differentiation. Thus, WEAO may be a promising herbal candidate to treat or prevent pathological bone diseases by regulating the balance between osteoclast and osteoblast activity. [ABSTRACT FROM AUTHOR]
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- 2016
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23. Anti-Inflammatory Effect of Rhapontici Radix Ethanol Extract via Inhibition of NF-κB and MAPK and Induction of HO-1 in Macrophages.
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Jeong, Yun Hee, Oh, You-Chang, Cho, Won-Kyung, Yim, Nam-Hui, and Ma, Jin Yeul
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ANTI-inflammatory agents ,THERAPEUTIC use of plant extracts ,MITOGEN-activated protein kinases ,NF-kappa B ,TRADITIONAL medicine ,MACROPHAGES - Abstract
Rhapontici Radix (RR) has been used in traditional medicine in East Asia and has been shown to have various beneficial effects. However, its biological properties or mechanism on inflammation-related diseases is unknown. The goal of this study was to determine the anti-inflammatory activity and underlying molecular mechanisms of Rhapontici Radix ethanol extract (RRE). The inhibitory effect of RRE on the production of NO, cytokines, inflammatory-related proteins, and mRNAs in LPS-stimulated macrophages was determined by the Griess assay, ELISA, Western blot analysis, and real-time RT-PCR, respectively. Our results indicate that treatment with RRE significantly inhibited the secretion of NO and inflammatory cytokines in RAW 264.7 cells and mouse peritoneal macrophages without cytotoxicity. We also found that RRE strongly suppressed the expression of iNOS and COX-2 and induced HO-1 expression. It also prevented nuclear translocation of NF-κB by inhibiting the phosphorylation and degradation of IκBα. Furthermore, the phosphorylation of MAPKs in LPS-stimulated RAW 264.7 cells was significantly inhibited by RRE. These findings suggest that RRE may operate as an effective anti-inflammatory agent by inhibiting the activation of NF-κB and MAPK signaling pathways and inducing HO-1 expression in macrophages. Our results suggest that RRE has potential value as candidate to inflammatory therapeutic phytomedicine. [ABSTRACT FROM AUTHOR]
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- 2016
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24. A Novel Herbal Medicine, KIOM-C, Induces Autophagic and Apoptotic Cell Death Mediated by Activation of JNK and Reactive Oxygen Species in HT1080 Human Fibrosarcoma Cells.
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Kim, Aeyung, Im, Minju, Yim, Nam-Hui, Kim, Taesoo, and Ma, Jin Yeul
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HERBAL medicine ,AUTOPHAGY ,APOPTOSIS ,C-Jun N-terminal kinases ,REACTIVE oxygen species ,FIBROSARCOMA ,CANCER cells - Abstract
KIOM-C was recently demonstrated to have anti-metastatic activity in highly malignant cancer cells via suppression of NF-κB-mediated MMP-9 activity. In addition, it was reported to be effective for clearance of the influenza virus by increasing production of anti-viral cytokines, such as TNF-α and IFN-γ, and efficacious in the treatment of pigs suffering from porcine circovirus-associated disease (PCVAD). In this study, we investigated whether KIOM-C induces cancer cell death and elucidated the underlying anti-cancer mechanisms. In addition, we examined whether KIOM-C oral administration suppresses in vivo tumor growth of HT1080 cells in athymic nude mice. We initially found that KIOM-C at concentrations of 500 and 1000 µg/ml caused dose- and time-dependent cell death in cancer cells, but not normal hepatocytes, to approximately 50% of control levels. At the early stage of KIOM-C treatment (12 h), cells were arrested in G
1 phase, which was accompanied by up-regulation of p21 and p27, down-regulation of cyclin D1, and subsequent increases in apoptotic and autophagic cells. Following KIOM-C treatment, the extent of caspase-3 activation, PARP cleavage, Beclin-1 expression, and LC3-II conversion was remarkably up-regulated, but p62 expression was down-regulated. Phosphorylation of AMPK, ULK, JNK, c-jun, and p53 was increased significantly in response to KIOM-C treatment. The levels of intracellular ROS and CHOP expression were also increased. In particular, the JNK-specific inhibitor SP600125 blocked KIOM-C-induced ROS generation and CHOP expression almost completely, which consequently almost completely rescued cell death, indicating that JNK activation plays a critical role in KIOM-C-induced cell death. Furthermore, daily oral administration of 85 and 170 mg/kg KIOM-C efficiently suppressed the tumorigenic growth of HT1080 cells, without systemic toxicity. These results collectively suggest that KIOM-C efficiently induces cancer cell death by both autophagy and apoptosis via activation of JNK signaling pathways, and KIOM-C represents a safe and potent herbal therapy for treating malignancies. [ABSTRACT FROM AUTHOR]- Published
- 2014
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25. Aqueous Extract of Bambusae Caulis in Taeniam Inhibits PMA-Induced Tumor Cell Invasion and Pulmonary Metastasis: Suppression of NF-κB Activation through ROS Signaling.
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Kim, Aeyung, Im, Minju, Yim, Nam-Hui, Jung, Young Pil, and Ma, Jin Yeul
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LUNG cancer treatment ,PLANT extracts ,CANCER cells ,NF-kappa B ,BAMBOO ,CYTOKINES ,ENZYME inhibitors ,CHINESE medicine ,ACTIVE oxygen in the body ,CELLULAR signal transduction - Abstract
Bamboo shavings (Bambusae Caulis in Taeniam, BCT) are widely used as a traditional Chinese medicine to control hypertension and cardiovascular disease, and to alleviate fever, vomiting, and diarrhea. It has been demonstrated that BCT reduces ovalbumin-induced airway inflammation by regulating pro-inflammatory cytokines, and decreases tumor growth in tumor-bearing mice. However, the effects of BCT on the metastatic potential of malignant cancer cells and the detailed mechanism of its anti-metastatic activity have not been examined previously. In this study, we investigated whether an aqueous extract of BCT (AE-BCT) reduces the metastatic potential of HT1080 cells, and elucidated the underlying anti-metastatic mechanism. In addition, we examined whether AE-BCT administration inhibits pulmonary metastasis of intravenously injected B16F10 cells in C57BL/6J mice. AE-BCT (50–250 µg/ml) dose-dependently suppressed colony-forming activity under anchorage-dependent and -independent growth conditions. Pretreatment with AE-BCT efficiently inhibited cell migration, invasion, and adhesion. AE-BCT also dramatically suppressed PMA-induced MMP-9 activity and expression by blocking NF-κB activation and ERK phosphorylation. Production of intracellular ROS, a key regulator of NF-κB-induced MMP-9 activity, was almost completely blocked by pretreatment with AE-BCT. Furthermore, daily oral administration of AE-BCT at doses of 50 and 100 mg/kg efficiently inhibited lung metastasis of B16F10 cells injected into the tail veins of C57BL/6J mice with no systemic toxicity. These results demonstrate that AE-BCT significantly reduced the metastatic activity of highly malignant cancer cells by suppressing MMP-9 activity via inhibition of ROS-mediated NF-κB activation. These results indicate that AE-BCT may be a safe natural product for treatment of metastatic cancer. [ABSTRACT FROM AUTHOR]
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- 2013
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26. Association of CYP26C1 Promoter Hypomethylation with Small Vessel Occlusion in Korean Subjects.
- Author
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Lee, Eun-Ji, Kim, Myung-Sunny, Yim, Nam-Hui, and Cha, Min Ho
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DNA methylation ,LEUCOCYTES ,PROMOTERS (Genetics) ,LACTATE dehydrogenase ,CYTOCHROME P-450 - Abstract
The risk factors for stroke, a fatal disease, include type two diabetes, hypertension, and genetic influences. Small vessel occlusion (SVO) can be affected by epigenetic alterations, but an association between SVO and the methylation of cytochrome P450 family 26 subfamily C member 1 (CYP26C1) has not been identified. In this study, we measured the level of DNA methylation in the CYP26C1 promoter and the 5′ untranslated region of 115 normal subjects and 56 patients with SVO in Korea. The DNA methylation level of each subject was measured by bisulfite amplicon sequencing, and statistical analysis was performed using the general linear model or Pearson's correlation. The average level of DNA methylation was markedly lower in patients with SVO than in normal subjects (20.4% vs. 17.5%). We found that the methylation of CYP26C1 has a significant positive correlation with blood parameters including white blood cells, hematocrit, lactate dehydrogenase, and Na+ in subjects with SVO. We predicted that binding of RXR-α and RAR-β might be affected by CYP26C1 methylation at CpG sites −246–237 and −294–285. These findings suggest that CYP26C1 methylation in the promoter region may be a predictor of SVO. [ABSTRACT FROM AUTHOR]
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- 2021
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27. Inhibition of Programmed Death Receptor-1/Programmed Death Ligand-1 Interactions by Ginsenoside Metabolites.
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Yim, Nam-Hui, Kim, Young Soo, Chung, Hwan-Suck, and Yang, Deok-Chun
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PROGRAMMED cell death 1 receptors , *IMMUNE checkpoint inhibitors , *METABOLITES , *GINSENOSIDES , *HYDROGEN bonding interactions , *HYDROPHOBIC interactions - Abstract
Evidence suggests that programmed death receptor-1/programmed death ligand-1 (PD-1/PD-L1) targeted inhibitors act as an immune checkpoint blockade, indicating that these compounds may be useful in cancer immunotherapy by inhibiting the immune response between T-cells and tumors. Previous studies have shown that ginsenosides can regulate the expression of PD-1 and PD-L1 in target diseases; however, it remains unknown whether ginsenosides act as a blockade of PD-1/PD-L1 interactions. In this study, we used competitive ELISA to investigate 12 ginsenosides for their ability to block PD-1/PD-L1 interactions. In addition, we performed a protein–ligand docking simulation and examined the hydrophobic interactions and hydrogen bonds formed at the interfaces between the ginsenosides and PD-L1/PD-1. Eight out of the 12 ginsenosides studied showed inhibition of PD-1/PD-L1 interactions at 35% at the maximum concentration (1 μM). Among them, Rg3 and Compound K (C-K) demonstrated the highest inhibitory effects. Rg3 and C-K were further identified for their interaction efficacy with PD-1/PD-L1, which supported our results demonstrating the blocking activity of these compounds against PD-1/PD-L1 binding interactions. Collectively, our findings suggest that some ginsenosides, including Rg3 and C-K, inhibit PD-1/PD-L1 binding interactions. Therefore, these compounds may prove useful as part of an overall immuno-oncological strategy. [ABSTRACT FROM AUTHOR]
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- 2020
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28. Hypermethylation of the TSPOAP1-AS1 Promoter May Be Associated with Obesity in Overweight/Obese Korean Subjects.
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Yim, Nam-Hui, Cha, Min Ho, and Kim, Myung Sunny
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BLOOD cholesterol , *DNA methylation , *BODY mass index , *ANTISENSE RNA , *OBESITY , *CHILDHOOD obesity , *ANTISENSE peptides - Abstract
Obesity is a major chronic disease associated with the risk of serious cardiovascular or endocrinal diseases, such as hypertension, diabetes, atherosclerosis and stroke. Considerable interest has been directed towards the potential effects of epigenetic variations in obesity. In this study, we evaluated DNA methylation level at the promoter region of the gene encoding TSPO-associated protein 1 antisense RNA 1 (TSPOAP1-AS1) in 80 overweight/obese subjects (body mass index (BMI) > 25) and 104 non-obese subjects who participated in the SOPI-Stroke study in Korea. DNA methylation was measured using bisulfite amplicon sequencing (BSAS). A general linear model or relative correlation was used to determine the effects of DNA methylation on obesity and obese phenotypes. Notably, the mean level of DNA methylation was significantly higher in the overweight/obese group than in the non-obese group (18.62% vs. 17.18%). Further analyses revealed significant positive correlations of the BMI, the serum total cholesterol and low-density lipoprotein cholesterol levels with the DNA methylation level (p = 0.0493, p = 0.003, and p = 0.0094, respectively). The study findings suggest an association between DNA methylation at the TSPOAP1-AS1 promoter and overweight/obesity. Accordingly, methylation in this promoter region might be a potential predictor of obesity. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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29. Antiplatelet Activity of Acylphloroglucinol Derivatives Isolated from Dryopteris crassirhizoma.
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Yim, Nam-Hui, Lee, Jung-Jin, Lee, BoHyoung, Li, Wei, Ma, Jin Yeul, and Efferth, Thomas
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ARACHIDONIC acid , *BLOOD platelet aggregation , *DICHLOROMETHANE , *COLUMN chromatography , *ETHYL acetate , *BLOOD platelet activation , *CARDIOMYOPATHIES - Abstract
Platelets are an important component of the initial response to vascular endothelial injury; however, platelet dysfunction induces the acute clinical symptoms of thrombotic disorders, which trigger severe cardiovascular diseases such as myocardial infarction, ischemia, and stroke. In this study, we investigated the Dryopteris crassirhizoma's antiplatelet activity. A water extract of D.crassirhizoma (WDC) was partitioned into dichloromethane (DCM), ethyl acetate, n-butyl alcohol, and water. Among these four fractions, the DCM fraction potently inhibited the collagen-stimulated platelet aggregation in a concentration-dependent manner. From this fraction, five different acylphloroglucinol compounds and one flavonoid were isolated by activity-guided column chromatography. They were identified by comparing their mass, 1H-, and 13C-NMR spectral data with those reported in the literature. Quantifying the six compounds in WDC and its DCM fraction by high-performance liquid chromatography (HPLC) revealed that butyryl-3-methylphloroglucinol (compound 4) was the most abundant in these samples. Additionally, butyryl-3-methylphloroglucinol showed the strongest inhibitory activity in the collagen- and arachidonic acid (AA)-induced platelet aggregation, with inhibition ratios of 92.36% and 89.51% in the collagen and AA-induced platelet aggregation, respectively, without cytotoxicity. On the active concentrations, butyryl-3-methylphloroglucinol significantly suppressed the convulxin-induced platelet activation. Regarding the structure–activity relationships for the five acylphloroglucinol compounds, our results demonstrated that the functional butanonyl, methoxy, and hydroxy groups in butyryl-3-methylphloroglucinol play important roles in antiplatelet activity. The findings indicate that acylphloroglucinols, including butyryl-3-methylphloroglucinol from D.crassirhizom, possess an antiplatelet activity, supporting the use of this species for antiplatelet remedies. [ABSTRACT FROM AUTHOR]
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- 2019
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30. Genetic toxicity of Epimedium koreanum Nakai.
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Hwang, Youn-Hwan, Yang, Hey Jin, Yim, Nam-Hui, and Ma, Jin Yeul
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ALTERNATIVE medicine , *BIOLOGICAL assay , *BIOLOGICAL models , *CHROMOSOME abnormalities , *GLYCOSIDES , *LIGNANS , *LIQUID chromatography , *MAMMALS , *MASS spectrometry , *MEDICINAL plants , *MUTAGENS , *PHYTOCHEMICALS , *PLANT extracts , *IN vitro studies , *IN vivo studies - Abstract
Ethnopharmacological relevance In Eastern Asia, E. koreanum Nakai (EKN) has traditionally been used as an aphrodisiac herbal medicine. However, there was no available information for its genotoxicity. This study was conducted to evaluate the genotoxic potentials of EKN. Materials and methods The phytochemicals of EKN were identified using liquid chromatography/tandem mass spectrometry (LC/MS/MS). Three standard battery of genotoxicity assay for bacterial reverse mutation, mammalian chromosomal aberration and in vivo micronuclei formation was employed. Results The LC/MS/MS analysis revealed four hydroxybenzoic acids, three lignans, and ten flavonoid glycosides in EKN. The bacterial reverse mutation assay revealed no mutagenic effects of EKN. Moreover, EKN did not show any clastogenic effects in the in vivo and in vitro assays. Conclusion EKN water extract was shown to be a non-genotoxic herbal medicine under the conditions tested in this study. [ABSTRACT FROM AUTHOR]
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- 2017
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31. Jageum-Jung improves 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in mice and suppresses pro-inflammatory chemokine production by inhibiting TNF-α/IFN-γ-induced STAT-1 and NFκB signaling in HaCaT cells.
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Yang, Ju-Hye, Do, Hyun Ju, Lee, Esther, Yim, Nam-Hui, Cho, Won-Kyung, Park, Kwang-Il, and Ma, Jin Yeul
- Subjects
- *
ANIMAL experimentation , *ATOPIC dermatitis , *CELLULAR signal transduction , *CHEMOKINES , *INFLAMMATORY mediators , *INTERFERONS , *INTERLEUKINS , *KERATINOCYTES , *MAST cells , *MEDICINAL plants , *ASIAN medicine , *MICE , *TRANSCRIPTION factors , *TUMOR necrosis factors , *DNA-binding proteins , *BENZENE derivatives , *PHARMACODYNAMICS - Abstract
Ethnopharmacological relevance Jageum-Jung (JGJ) is an oriental herbal formula comprising five herbs ( Melaphis chinensis Bell, Cremastra variabilis Nakai, Knoxia valerianoides Thorel, Euphorbia lathyris L., and Moschus moschiferus L.). It has been used for detoxification and treating cancer and inflammatory diseases in China, Japan, and Korea. However, the mechanism of action of JGJ on keratinocyte inflammatory response is poorly understood. Aim of the study In the present study, we investigated the anti-inflammatory mechanism of JGJ and studied the effects of JGJ on atopic dermatitis-like skin lesions in mice. Materials and methods We elucidated the anti-inflammatory and anti-inflammatory effects of JGJ on tumor necrosis factor-α/interferon-γ (TNF-α/IFN-γ)-treated human keratinocyte cells, IgE-sensitized RBL-2H3 cells, and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like mice, respectively. Results The results showed that JGJ suppressed the production and mRNA revels of IL-8, IL-6 and, conspicuously, both TARC and RANTES. JGJ inhibited nuclear translocation of the inflammatory transcription factors NFκB and STAT-1. Moreover, JGJ improved AD-like skin lesions in DNCB-treated mice and inhibited degranulation of mast cell. Conclusions The results of this study suggest that JGJ can be considered as a candidate agent for AD treatment. [ABSTRACT FROM AUTHOR]
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- 2018
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32. Anti-inflammatory effects of Forsythia suspensa in dextran sulfate sodium-induced colitis.
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Hwang, Youn-Hwan, Kim, Dong-Gun, Li, Wei, Yang, Hye Jin, Yim, Nam-Hui, and Ma, Jin Yeul
- Subjects
- *
ANTI-inflammatory agents , *ANIMAL experimentation , *COLITIS , *INTERLEUKINS , *MEDICINAL plants , *ASIAN medicine , *PROBABILITY theory , *TUMOR necrosis factors - Abstract
Ethnopharmacological relevance Forsythia suspensa Fructus (FS) is used to treat various inflammatory disorders in traditional Oriental medicine, including gastrointestinal diseases, but its therapeutic potential in ulcerative colitis is unclear. Thus, we investigated any potential therapeutic effects of FS against intestinal inflammation and the bioactive constituents in FS. Materials and methods After the induction of colitis using 3% dextran sulfate sodium, FS (100 mg/kg/day) was administered orally during the experimental period. We evaluated body weight, bloody diarrhea, colon length, and pro-inflammatory cytokine levels. Subsequently, the bioactive constituents of FS were identified using UPLC/MS/MS. Results FS significantly decreased the body weight loss, colon length shortening, and tumor necrosis factor-α and interleukin-6 elevations induced by colitis compared with the negative control ( P < 0.05). Moreover, FS improved the colitis-induced histopathological damage to the colon, including epithelial necrosis, infiltration of inflammatory cells, ulceration, and submucosal edema. In phytochemical analyses, 7 flavonoids, 9 lignans, 13 phenolics, and 2 triterpenes were identified by comparison with the retention times and mass fragmentations of authentic standards. Conclusions We demonstrated beneficial effects of FS and its constituents, suggesting their potential for treatment of intestinal inflammation. These data could provide useful information for managing ulcerative colitis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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