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Using the CiteSpace visual literature analysis tool, 802 relevant literature on dragon boat racing research were analyzed to map the knowledge structure of research hotspots, core author groups and research institutions from 1980-2022 in CNKI General Library. The results show that dragon boat racing research has experienced a stagnant period, a recovery period and a rapid development period, and it was expected that after 2023, dragon boat racing research would usher in new development opportunities. Dai Yu, Wang Saishi and Wang Junqi were the main authors of publications, but there was not much cooperation among research authors, mainly relying on academic relationships and not much cross-regional cooperation; Guangxi University for Nationalities, Shanxi Normal University and Minzu University of China were the institutions with more publications, and universities were the foundation of dragon boat culture research. Keyword co-occurrence analysis formed a total of 765 nodes with 1372 connections, (density = 0.0047), S=0.9124, which was close to 1, indicating that the homogeneity of the network was high and the research perspectives in the field of dragon boat racing tend to be diversified. Analyzed by the time of appearing keywords, dragon boat racing research shows a trend of migrating from cultural research to competitive sports. [ABSTRACT FROM AUTHOR]

A Z-scheme BNO/BSO composite photocatalyst has been successfully prepared using an in situ solvothermal method. The phase component, microstructure and optical properties of the as-prepared samples were characterized using X-ray diffractometry, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, photoluminescence spectroscopy and other means. The photocatalytic performance of the BNO/BSO composite was evaluated via the degradation of the typical antibiotic tetracycline (TC) under hydrogen peroxide assistance and visible light irradiation. The "cata + H2O2 + vis" system shows the best photocatalytic activity, and its apparent rate constant reaches 0.03164 min−1, which is 4.9 times and 5.7 times that of the "cata + vis" system and the "cata + H2O2" system, respectively. Compared with pristine that of BSO alone, the reaction rate constant of the 15% BNO/BSO composite increases 1.8 times. The enhanced photocatalytic activity is attributed to the construction of a unique Z-scheme-type heterojunction, which effectively suppresses the recombination of electron–hole pairs. In addition, the addition of H2O2 promotes the generation of more active species. Moreover, a possible photocatalytic degradation mechanism is also further proposed. [ABSTRACT FROM AUTHOR]

Previous studies reported that the Myoz1 gene plays a crucial role in signal transduction and muscle fibre type differentiation. This finding suggests that the Myoz1 gene is a potential candidate for affecting carcase and meat quality traits in animals. Therefore, this study aimed to detect genetic variations in Avian and Yellow Bantam chickens and to evaluate the effects of these variations on economically important carcase and meat-quality traits. Expression analysis using quantitative real-time PCR indicated that Myoz1 was broadly expressed in all four studied tissues (liver, heart, breast muscle and leg muscle). Expression of Myoz1 was significantly higher in both leg muscle and breast muscle compared to the liver and heart. Then, using direct sequencing, a total of five single nucleotide polymorphisms (SNPs) were identified within the Myoz1 gene in two breeds of chickens. These breeds included three non-synonymous SNPs (SNP1: g. 16022512 G > T, SNP3: g. 16022560 C > T and SNP5: g.16023903 A > G) corresponding to A103S, H119Y and S189G, respectively. Statistical analyses indicated that SNP2 (g. 16022529 T > C), SNP3, SNP4 (g.16023878 A > C) and SNP5 significantly influenced some carcase and meat quality traits. Meanwhile, we did not find any association between the constructed haplotype and carcase traits in the Avian nor Yellow Bantam chickens. In conclusion, SNPs in the Myoz1 gene could be used for marker-assisted selection in chicken breeding. [ABSTRACT FROM AUTHOR]

Embryonic muscle development and fibre type differentiation has always been a topic of great importance due to its impact on both human health and farm animal financial values. Myozenin3 (Myoz3) is an important candidate gene that may regulate these processes. In the current study, we knocked down and overexpressed Myoz3 in chicken embryonic fibroblasts (CEFs) and chicken myoblasts, then utilized RNA-seq technology to screen genes, pathways and biological processes associated with Myoz3. Multiple differentially expressed genes were identified, including MYH10, MYLK2, NFAM1, MYL4, MYL9, PDZLIM1; those can in turn regulate each other and influence the development of muscle fibres. Gene ontology (GO) terms including some involved in positive regulation of cell proliferation were enriched. We further validated our results by testing the activity of cells by cell counting kit-8(CCK-8) and confirmed that under the condition of Myoz3 overexpression, the proliferation rate of CEFs and myoblasts was significantly upregulated, in addition, expression level of fast muscle specific gene was also significantly upregulated in myoblasts. Pathway enrichment analysis revealed that the PPAR (Peroxisome Proliferator-Activated Receptor) pathway was enriched, suggesting the possibility that Myoz3 regulates muscle fibre development and differentiation through the PPAR pathway. Our results provide valuable evidence regarding the regulatory functions of Myoz3 in embryonic cells by screening multiple candidate genes, biological processes and pathways associated with Myoz3. [ABSTRACT FROM AUTHOR]

Based on the monitoring results ol environmental quality in the sea area near the Aojiang estuary in Wenzou during 2010-2012, the present situation of environmental quality in the sea area was analyzed and evaluated. The results show that pH, DO. CODMn, petroleum, and heavy metals (Cu, Pb, Zn, Cd, Hg, As and Cr) in the sea area near the Aojiang estuary did not exceed the second-class standard of Seawater Quality Standard (GB 3097-1997), but both inorganic nitrogen and reactive phosphate in the sea area exceeded the second-class standard seriously, and the water quality of the sea area was in an eutrophic state; domestic sewage and fertilizer loss were the main sources ol nitrogen and phosphorus. The standard index of most evaluation factors of sediment quality except for Zn was smaller than 1, meeting the demands of sediment quality for environmental protection. [ABSTRACT FROM AUTHOR]

Myozenin3 (Myoz3) has been reported to bind multiple Z-disc proteins and hence play a key role in signal transduction and muscle fiber type differentiation. The purpose of current study is to better understand the basic characteristics of Myoz3. Firstly, we cloned the ORF (open reading frame) of the Myoz3 gene. AA (amino acid) sequence analysis revealed that the Myoz3 gene encodes a 26 kDa protein which have 97% identities with that of turkey. Expression profiling showed that Myoz3 mRNA is mainly expressed in leg muscle and breast muscle. Furthermore, we investigated Myoz3 gene polymorphisms in two broiler breeds, the Yellow Bantam (YB) and the Avian. Five SNPs (single nucleotide polymorphisms) were identified in the YB breed and 3 were identified in the Avian breed. Genotypes and haplotype were constructed and their associations with carcass traits were analyzed. In the YB breed, c.516 C>T had a strong effect on both shank bone length and the L⁎ value of breast muscle, and the H1H3 diplotype had the highest FC compared to other diplotypes. The markers identified in this study may serve as useful targets for the marker-assisted selection (MAS) of growth and meat quality traits in chickens. [ABSTRACT FROM AUTHOR]

internal standard method based on gas chromatography-electron capture detector (GC-ECD), the contents of persistent organochlorine pollutants polychorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in eggs of seabirds (Catharacta lonnbergi, Catharacta maccormicki, Pygoscelis papua and Macronectes giganteus) breeding on King George Island, Antarctica were detected, and their ecological environment significance was discussed. The results showed that the recovery of various compounds ranged from 68.6% to 90.8% ; relative standard deviation (RSD) was 3.6%, and the lowest detection limits of PCBs and OCPs were 0.8 -16 and 2 - 1 2 pg respectively, which could meet the demands for the residue analysis of trace persistent organochlorine pollutants in the eggs of Antarctic seabirds. The contents of PCBs, DDTs, HCB and HCHs in the eggs of Antarctic seabirds were 0.5 -515.5, 2.0 -304.4, 0.5 -70.5, and 0 . 5 - 2 . 0 ng/g respectively. In addition, the accumulation of PCBs and OCPs in the skuas was enhanced gradually through the food chain. The maximums of persistent organochlorine pollutants appeared in the eggs of C. lonnbergi, followed by the eggs of C. maccomicki. The detection of OCPs and PCBs in the eggs of Antarctic seabirds was not interfered by other compounds containing chlorine, showing that the eXraction, purification and detection method was suitable for the analysis of OCPs and PCBs in the eggs of Antarctic seabirds and has high sensitivity and reliability. [ABSTRACT FROM AUTHOR]

The ubiquitin E3 ligase RNF220 and its co-factor ZC4H2 are required for multiple neural developmental processes through different targets, including spinal cord patterning and the development of the cerebellum and the locus coeruleus. Here, we explored the effects of loss of ZC4H2 and RNF220 on the proliferation and differentiation of neural stem cells (NSCs) derived from mouse embryonic cortex. We showed that loss of either ZC4H2 or RNF220 inhibits the proliferation and promotes the differentiation abilities of NSCs in vitro. RNA-Seq profiling revealed 132 and 433 differentially expressed genes in the ZC4H2−/− and RNF220−/− NSCs, compared to wild type (WT) NSCs, respectively. Specifically, Cend1, a key regulator of cell cycle exit and differentiation of neuronal precursors, was found to be upregulated in both ZC4H2−/− and RNF220−/− NSCs at the mRNA and protein levels. The targets of Cend1, such as CyclinD1, Notch1 and Hes1, were downregulated both in ZC4H2−/− and RNF220−/− NSCs, whereas p53 and p21 were elevated. ZC4H2−/− and RNF220−/− NSCs showed G0/G1 phase arrest compared to WT NSCs in cell cycle analysis. These results suggested that ZC4H2 and RNF220 are likely involved in the regulation of neural stem cell proliferation and differentiation through Cend1. [ABSTRACT FROM AUTHOR]