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Your search keyword '"Yasuhiro Kakimoto"' showing total 3 results
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3 results on '"Yasuhiro Kakimoto"'

1. Supported Lipid Bilayers of Escherichia coli Extracted Lipids and Their Calcium Dependence

Author

Yasuhiro Kakimoto and Ryugo Tero

Subjects
supported lipid bilayer, E. coli lipid, lipid organization, atomic force microscopy, fluorescence microscopy, fluorescence recovery after photobleaching, Technology
Abstract

Formation of supported lipid bilayer (SLB) and additional structures of Escherichia coli (E. coli) lipids were investigated with fluorescence microscopy and atomic force microscopy. Ca2+ in the aqueous phase with concentration above 2 mM was necessary for the formation of SLB. Additional lipid structures, string-like structures, the second lipid bilayer, and multilayer stacking appeared on the first layer SLB depending on Ca2+ concentration. The bridging effect of Ca2+ between the negatively charged E. coli lipid bilayers and substrate is the dominant factor determining the two-dimensional and three-dimensional morphology of the E. coli lipid bilayer membranes.

Published
2018
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2. Morphology and Physical Properties of Hydrophilic-Polymer-Modified Lipids in Supported Lipid Bilayers

Author

Yoshihiro Tachihara, Yoshiaki Okamoto, Keisuke Miyazawa, Ryugo Tero, Yasuhiro Kakimoto, and Takeshi Fukuma

Subjects
0301 basic medicine, Morphology (linguistics), Polymers, Lipid Bilayers, macromolecular substances, 02 engineering and technology, Polyethylene glycol, Microscopy, Atomic Force, 03 medical and health sciences, chemistry.chemical_compound, PEG ratio, Microscopy, Electrochemistry, Fluorescence microscope, General Materials Science, Lipid bilayer, Spectroscopy, Chemistry, technology, industry, and agriculture, Fluorescence recovery after photobleaching, Surfaces and Interfaces, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Lipids, 030104 developmental biology, Membrane, Microscopy, Fluorescence, Biophysics, lipids (amino acids, peptides, and proteins), 0210 nano-technology, Hydrophobic and Hydrophilic Interactions
Abstract

Lipid molecules such as glycolipids that are modified with hydrophilic biopolymers participate in the biochemical reactions occurring on cell membranes. Their functions and efficiency are determined by the formation of microdomains and their physical properties. We investigated the morphology and properties of domains induced by the hydrophilic-polymer-modified lipid applying a polyethylene glycol (PEG)-modified lipid as a model modified lipid. We formed supported lipid bilayers (SLBs) using a 0–10 mol % range of PEG-modified lipid concentration (CPEG). We studied their morphology and fluidity by fluorescence microscopy, the fluorescence recovery after photobleaching method, and atomic force microscopy (AFM). Fluorescence images showed that domains rich in the PEG-modified lipid appeared and SLB fluidity decreased when CPEG ≥ 5%. AFM topographies showed that clusters of the PEG-modified lipid appeared prior to domain formation and the PEG-lipid-rich domains were observed as depressions. Frequency-modulati...

Published
2018
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