1. Identification and characterization of multipotential stem cells in immortalized normal ovarian surface epithelial cells
- Author
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Hou Lin, Hong Hanqing, Cao Wenjiao, Wei Liutong, Weng Lichun, Yuan Shuang, Xiao Chengqi, Zhang Qiuwan, Wang Qian, and Lai Dongmei
- Subjects
ovarian surface epithelial cell ,transcriptome ,multipotential stem cell ,oocyte ,repair function ,Biochemistry ,QD415-436 ,Genetics ,QH426-470 - Abstract
The ovarian surface epithelium (OSE) is a single layer of squamous-to-cuboidal epithelial cells that experience repetitive ovulatory rupture and subsequent repair. However, the characteristics of human immortalized ovarian surface epithelial cells (IOSE80) remain elusive. This study aims to determine whether IOSE80 cells have the characteristics of stem cell proliferation and multilineage differentiation and their application in regenerative medicine. IOSE80 cells are sequenced by high-throughput transcriptome analysis, and 5 sets of public data are used to compare the differences between IOSE80 cells and bone marrow mesenchymal stem cells, pluripotent stem cells, and oocytes in transcriptome profiling. The IOSE80 cells present a cobblestone-like monolayer and express the epithelial cell marker KRT18; the stem cell markers IFITM3, ALDH1A1, and VIM; lowly express stem cell marker LGR5 and germ cell markers DDX4 and DAZL. In addition, the GO terms “regulation of stem cell proliferation”, “epithelial cell proliferation”, etc., are significantly enriched (P
- Published
- 2024
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