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The geochemical analysis and experimental simulation are comprehensively used to systematically study the hydrocarbon generation material, organic matter enrichment and hydrocarbon generation model of Paleogene source rock in the Western Qaidam Depression, Qaidam Basin, NW China. Three main factors result in low TOC values of saline lacustrine source rock of the Qaidam Basin: relatively poor nutrient supply inhibits the algal bloom, too fast deposition rate causes the dilution of organic matter, and high organic matter conversion efficiency causes the low residual organic carbon. For this type of hydrogen-rich organic matter, due to the reduction of organic carbon during hydrocarbon generation, TOC needs to be restored based on maturity before evaluating organic matter abundance. The hydrocarbon generation of saline lacustrine source rocks of the Qaidam Basin is from two parts: soluble organic matter and insoluble organic matter. The soluble organic matter is inherited from organisms and preserved in saline lacustrine basins. It generates hydrocarbons during low-maturity stage, and the formed hydrocarbons are rich in complex compounds such as NOS, and undergo secondary cracking to form light components in the later stage; the hydrocarbon generation model of insoluble organic matter conforms to the traditional “Tissot” model, with an oil generation peak corresponding to Ro of 1.0%.

Ultra-low permeability–tight sandstone (gravel) gas reservoir is an important exploration target for natural gas both domestically and internationally. As a crucial oil and gas exploration basin in China, the Bohai Bay Basin has discovered tight sandstone (gravel) gas in every depression. However, the distribution, origin, and accumulation model of tight sandstone (gravel) gas reservoirs were not systematically studied by previous researchers, who instead mainly concentrated on depressions from the basin-wide perspective. The research conclusively demonstrates that ultra-low permeability–tight sandstone (gravel) gas reservoirs are widely distributed in the Bohai Bay Basin, covering various depressions and multiple strata of Paleozoic, Cenozoic, and Upper Paleozoic coal-formed gas as well as Paleogene oil-type gas. The Upper Paleozoic ultra-low permeability–tight sandstone gas reservoirs are mainly distributed in residual Upper Paleozoic strata of the Huanghua, Linqing, and Jiyang depressions. Most of those reservoirs, which are located in uplifted sag zones, are structural gas reservoirs, and because the strata were buried deeply in the past, most of the reservoirs have worse physical properties. This natural gas is typical coal-formed gas originating in Carboniferous and Permian, and others are Paleogene oil-type gas. Paleogene ultra-low permeability–tight gas reservoirs are widely distributed in all depressions, including the multiple strata in the Kongdian Formation and the second, third. and fourth members of the Shahejie Formation (Es2, Es3, and Es4); The gas reservoirs are located in the uplift, slope, and steep slope zones of the sags, including a variety of trap types, such as lithology, lithology-structure, structure reservoirs, and others. Sandstone reservoirs often found in the Es2 and Es3 are distributed in gentle slopes, whereas sandstone and glutenite reservoirs can be found in the Es4 and Kongdian Formation (Ek), with the glutenites mainly distributed in steep slope area. Since the Paleogene is a continuous deposit, burial depth appears to be a controlling factor in the reservoirs physical properties. Densification threshold depths for distinct depressions range from 3200 m to 4000 m. The majority of the natural gas is gas formed from Paleogene oil-type, while some of it is gas formed from Upper Paleozoic coal. Four different hydrocarbon accumulation models have been developed in this area in accordance with the relationship between gas sources and reservoirs. These models include migration along fault with old source rock and new reservoir, near-source accumulation with new source rock and new reservoir, distant-source accumulation with old source rock and old reservoir, and migration along fault with new source rock and old reservoir, of which the first two are the main hydrocarbon accumulation models. Two sets of Carboniferous–Permian and Paleogene strata are developed, and the remaining natural gas resources have great potential in the Bohai Bay Basin. The Carboniferous–Permian beneficial zone of secondary hydrocarbon generation, the structures adjacent to the margin of the Paleogene gas generation center, and the sweet spot in lithology reservoirs would all be promising areas for further exploration. The research is of instructive significance for tight sandstone (gravel) gas exploration in the Bohai Bay Basin.

Breakthroughs have been made for the natural gas exploration of the Middle Devonian and the Middle Permian formations in the Sichuan Basin in recent years. Nevertheless, its natural gas source is still not clear, which then affects the next exploration deployment decision. A comprehensive study is conducted on the geochemical characteristics of the Middle Devonian and the Middle Permian natural gas, the biomarkers of reservoir bitumen, and the source rocks. The results show that these natural gases are secondary cracking dry gas that includes more than 86% of methane content, a small amount of ethane, propane, and a non-hydrocarbon gas (e.g., nitrogen, carbon dioxide, and hydrogen sulfide). The natural gas maturity varies slightly in different regions. The carbon and hydrogen isotope values of natural gas are as follows: δ13C1 ranges from −35.7‰ to −27.3‰, δ13C2 ranges from −38.7‰ to −26.6‰, δ13C3 ranges from −37‰ to −26.5‰, δ2HCH4 ranges from −141‰ to −138‰, and δ2HC2H6 ranges from −164‰ to −112‰. The isotope values indicate that the genetic type of natural gas is the sapropelic type and the mixture type is dominated by sapropelic type. The difference between natural gas values of δ13C1, δ13C2, and δ2HCH4 in different regions is related to the contribution ratio of source rocks from different ages. The gases of the Middle Devonian and the Middle Permian of the Shuangyushi structure in Northwestern Sichuan, the Middle Permian of Southwestern Sichuan, and the Middle Permian of Paleo-uplift in Central Sichuan are mainly derived from the Cambrian Qiongzhusi Formation and the Middle-Lower Permian source rocks. The Middle-Upper Permian natural gases of the volcanic reservoir in Southwestern Sichuan is mainly from the Qiongzhusi source rock. The Middle Permian natural gases of the Hewanchang structure in Northwestern Sichuan, Eastern Sichuan, and Southern Sichuan are mainly derived from the Silurian Longmaxi source rock. The research results have important guiding significance for developing exploration and deployment solutions for natural gas of the Middle Devonian and the Middle Permian formation in the Sichuan Basin.

Jurassic coal-measure strata are widely developed in Northwest China and mainly distributed in the Tarim Basin, Junggar Basin, Qaidam Basin, and Tuha Basin. The Jurassic coal-formed gas resources are abundant. Although the Jurassic strata in Northwest China have similar tectonic and sedimentary environments, the geochemical characteristics of the coal-formed gas formed in Northwest China have both similarities and differences. Therefore, analyzing and summarizing the geochemical characteristics and exploration potential of the coal-formed gas in the Jurassic petroleum systems in different basins are of great significance for searching for different types of coal-formed gases. Through comprehensive comparison and analysis, it is believed that the drying coefficient of Jurassic coal-formed gases in western China present three types: namely the single peak type of wet gas, the double peak type wet gas-dry gas and the single peak type dry gas; the distribution range of δ13C1 is large, corresponding to the sequence of source rocks from low maturity to over-mature; the partial isotopic inversion of C2 and C3 is mainly caused by the mixed source and biological actions; source rock maturity is the main reason causing the geochemical differences of natural gases in different regions. The Jurassic coal-formed gas in Northwest China has large exploration potential, and the exploration degree of structural gas reservoirs is relatively high. The structural-lithological and lithological gas reservoirs, as well as the main exploration reservoir types for Jurassic coal-formed gas in Northwest China; the Jurassic system in the Kuqa Depression of Tarim Basin, Cretaceous-Jurassic system in the southern margin of the Junggar Basin, the Jurassic high maturity coal-formed gas in the Tuha Basin and the Jurassic high maturity–post-mature coal-formed gas in the Qaidam Basin have great exploration potential, which are important exploration fields for Jurassic coal-formed gas in Northwest China in the future. Keywords: Northwest China, Jurassic coal-formed gas, Geochemical characteristics, Exploration potential

The Kuche Depression is considered as the most important gas resource potential and gas exploring area with great gas resource potential and prospect in the Tarim Basin. Based on geochemical experimental analyses and comprehensive geological studies, the general geochemical characteristics of molecular and isotope compositions of rare gases as well as hydrocarbon gases and nonhydrocarbon gases are comparatively studied in the Kuche and Southwestern Depressions. Then, their genetic types are separately identified and gas originations are comprehensively discussed. The main results are as follows. (1) Gas fields in the Kuche Depression have a higher methane abundance, accompanied with low N2 and CO2 abundances, but the Akemomu gas field in the Southwestern Depression has a relatively lower average methane abundance, accompanied with high average N2 and CO2 abundances. The helium abundance of natural gases in gas fields from the Kuche Depression general has 1 order of magnitude higher than the air value. Comparatively, it has more than 2 orders of magnitude higher than the atmospheric value in the Akemomu gas field from the Southwestern Depression. The neon, argon, krypton, and xenon abundances in both Kuche and Southwestern Depressions are lower than the corresponding air values. (2) Natural gases from gas fields in the Kuche Depression and the Southwestern Depressions are generally typical coal-formed gases. The rare gases in the Kuche Depression have typical crustal genesis, mainly deriving from the radioactive decay of elements in the crust, while in the Akemomu gas field from the Southwestern Depression, the rare gases have main crustal genesis with a proportion of 92.5%, probably accompanied with a little mantled genetic contribution. (3) Natural gases in the Kuche Depression are generally derived from coal measure source rocks of Jurassic and Triassic, which principally originated from Jurassic in strata period and coals in source rock types. The Jurassic source rocks account for 55%-75% and the Triassic source rocks account for 25%-45% approximately, while coals occupy 68% and mudstones occupy 32% separately. Natural gases from the Akemomu gas field in the Southwestern Depression mainly originated from humic mudstones of marine and continental transitional source rocks of Carboniferous to Permian.

Giardia duodenalis is a zoonotic intestinal protozoan parasite that may cause host diarrhea and chronic gastroenteritis, resulting in great economic losses annually and representing a significant public health burden across the world. However, thus far, our knowledge on the pathogenesis of Giardia and the related host cell responses is still extensively limited. The aim of this study is to assess the role of endoplasmic reticulum (ER) stress in regulating G0/G1 cell cycle arrest and apoptosis during in vitro infection of intestinal epithelial cells (IECs) with Giardia. The results showed that the mRNA levels of ER chaperone proteins and ER-associated degradation genes were increased and the expression levels of the main unfolded protein response (UPR)-related proteins (GRP78, p-PERK, ATF4, CHOP, p-IRE1, XBP1s and ATF6) were increased upon Giardia exposure. In addition, cell cycle arrest was determined to be induced by UPR signaling pathways (IRE1, PERK and ATF6) through upregulation of p21 and p27 levels and promotion of E2F1-RB complex formation. Upregulation of p21 and p27 expression was shown to be related to Ufd1-Skp2 signaling. Therefore, the cell cycle arrest was induced by ER stress when infected with Giardia. Furthermore, the apoptosis of the host cell was also assessed after exposure to Giardia. The results indicated that apoptosis would be promoted by UPR signaling (PERK and ATF6), but would be suppressed by the hyperphosphorylation of AKT and hypophosphorylation of JNK that were modulated by IRE1 pathway. Taken together, both of the cell cycle arrest and apoptosis of IECs induced by Giardia exposure involved the activation of the UPR signaling. The findings of this study will deepen our understanding of the pathogenesis of Giardia and the associated regulatory network.

Giardia duodenalis, the causative agent of giardiasis, is among the most important causes of waterborne diarrheal diseases around the world. Giardia infection may persist over extended periods with intestinal inflammation, although minimal. Cyclooxygenase (COX)-2 is well known as an important inducer of inflammatory response, while the role it played in noninvasive Giardia infection remains elusive. Here we investigated the regulatory function of COX-2 in Giardia-induced pro-inflammatory response and defense-related nitric oxide (NO) generation in macrophage-like cell line, and identified the potential regulators. We initially found that Giardia challenge induced up-regulation of IL-1β, IL-6, TNF-α, prostaglandin (PG) E2, and COX-2 in macrophages, and pretreatment of the cells with COX-2 inhibitor NS398 reduced expressions of those pro-inflammatory factors. It was also observed that COX-2 inhibition could attenuate the up-regulated NO release and inducible NO synthase (iNOS) expression induced by Giardia. We further confirmed that Giardia-induced COX-2 up-regulation was mediated by the phosphorylation of p38 and ERK1/2 MAPKs and NF-κB. In addition, inhibition of reactive oxygen species (ROS) by NAC was shown to repress Giardia-induced activation of MAPK/NF-κB signaling, up-regulation of COX-2 and iNOS, increased levels of PGE2 and NO release, and up-expressions of IL-1β, IL-6, and TNF-α. Collectively, in this study, we revealed a critical role of COX-2 in modulating pro-inflammatory response and defense-related NO production in Giardia-macrophage interactions, and this process was evident to be controlled by ROS-dependent activation of MAPK/NF-κB signaling. The results can deepen our knowledge of anti-Giardia inflammatory response and host defense mechanisms.

Urban air quality mapping has been widely applied in urban planning, air pollution control and personal air pollution exposure assessment. Urban air quality maps are traditionally derived using measurements from fixed monitoring stations. Due to high cost, these stations are generally sparsely deployed in a few representative locations, leading to a highly generalized air quality map. In addition, urban air quality varies rapidly over short distances (2 by 100 m and 1 h resolution for fine-grained air quality mapping. The CLADF model exploits deep forest to construct a local model for each cluster consisting of nearest neighbor measurements in contextual feature space, and considers traffic flow as an important contextual feature. Extensive validation experiments were conducted using mobile NO2 measurements collected by 17 postal vans equipped with low-cost sensors operating in Antwerp, Belgium. The experimental results demonstrate that the CLADF model achieves the lowest RMSE as well as advances in accuracy and correlation, compared with various benchmark models, including random forest, deep forest, extreme gradient boosting and support vector regression.