Search

In this article, we study a p-Laplacian Schrödinger-Poisson system involving a parameter q≠0q\ne 0 in bounded domains. By using the Nehari manifold and the fibering method, we obtain the non-existence and multiplicity of nontrivial solutions. On one hand, there exists q*>0{q}^{* }\gt 0 such that only the trivial solution is admitted for q∈(q*,+∞).q\in \left({q}^{* },+\infty ). On the other hand, there are two positive solutions existing for q∈(0,q0*+ε)q\in \left(0,{q}_{0}^{* }+\varepsilon ), where ε>0\varepsilon \gt 0 and q0*+ε

Based on neural machine translation, this article introduced the ConvS2S system and transformer system, designed a semantic sharing combined transformer system to improve translation quality, and compared the three systems on the NIST dataset. The results showed that the operation speed of the semantic sharing combined transformer system was the highest, reaching 3934.27 words per second; the BLEU value of the ConvS2S system was the smallest, followed by the transformer system and the semantic sharing combined transformer system. Taking NIST08 as an example, the BLEU values of the designed system were 4.74 and 1.49 higher than the other two systems. The analysis of examples showed that the semantic sharing combined transformer had higher translation quality. The experimental results show that the proposed system is reliable in English content translation and can be further promoted and applied in practice.

Background: Mesenchymal stem cells (MSCs) are increasingly recognized for their regenerative potential. However, their clinical application is hindered by their inherent variability, which is influenced by various factors, such as the tissue source, culture conditions, and passage number. Methods: MSCs were sourced from clinically relevant tissues, including adipose tissue-derived MSCs (ADMSCs, n = 2), chorionic villi-derived MSCs (CMMSCs, n = 2), amniotic membrane-derived MSCs (AMMSCs, n = 3), and umbilical cord-derived MSCs (UCMSCs, n = 3). Passages included the umbilical cord at P0 (UCMSCP0, n = 2), P3 (UCMSCP3, n = 2), and P5 (UCMSCP5, n = 2) as well as the umbilical cord at P5 cultured under low-oxygen conditions (UCMSCP5L, n = 2). Results: We observed that MSCs from different tissue origins clustered into six distinct functional subpopulations, each with varying proportions. Notably, ADMSCs exhibited a higher proportion of subpopulations associated with vascular regeneration, suggesting that they are beneficial for applications in vascular regeneration. Additionally, CMMSCs had a high proportion of subpopulations associated with reproductive processes. UCMSCP5 and UCMSCP5L had higher proportions of subpopulations related to female reproductive function than those for earlier passages. Furthermore, UCMSCP5L, cultured under low-oxygen (hypoxic) conditions, had a high proportion of subpopulations associated with pro-angiogenic characteristics, with implications for optimizing vascular regeneration. Conclusions: This study revealed variation in the distribution of MSC subpopulations among different tissue sources, passages, and culture conditions, including differences in functions related to vascular and reproductive system regeneration. These findings hold promise for personalized regenerative medicine and may lead to more effective clinical treatments across a spectrum of medical conditions. [ABSTRACT FROM AUTHOR]

Background: Homocysteine (Hcy) has been found to be closely related to the occurrence of diabetes mellitus (DM) and is considered as one of the risk factors of DM. However, Hcy alone is not enough as a factor to predict DM, and our study analyzed and determined the relationship between the main metabolites involved in the Hcy metabolic pathway and DM. Methods: A total of 48 clinical samples were collected, including 18 health control samples and 30 DM samples. All standards and samples were detected by LC-QTOF-MS. Multivariate statistical analysis and k-means cluster analysis were performed to screen and confirm the metabolites significantly correlated with DM. Results: A total of 13 metabolites of the Hcy metabolic pathway were detected in the samples. The content of Hcy, cysteine, taurine, pyridoxamine, methionine, and choline were significantly increased in the DM group (p < 0.05). Hcy, choline, cystathionine, methionine, and taurine contributed significantly to the probabilistic principal component analysis (PPCA) model. The odds ratios (OR) of Hcy, cysteine, taurine, methionine, and choline were all greater than one. K-means cluster analysis showed that the Hcy, taurine, methionine, and choline were significantly correlated with the distribution of glucose values (divided into four levels: 10.5–11.7 mmol/L, 7.7–9.7 mmol/L, 6.0–6.9 mmol/L, and 5.0–5.9 mmol/L, respectively). Conclusion: Hcy, taurine, methionine, and choline can be used as risk factors for diabetes diagnosis and are expected to be used for the assessment of diabetes severity. [ABSTRACT FROM AUTHOR]

Polycystic ovary syndrome (PCOS), characterized by polycystic ovarian morphology, ovarian follicular maturation arrest, and hormonal disorders, affects numerous women in the reproductive age worldwide. A recent study has found that mitochondria are likely to play an essential role in oocyte quality. However, it is still unclear whether oocyte development failure is associated with mitochondria in patients with PCOS. We analyzed the single-cell RNA sequencing data from the previous study, including data from 14 oocytes from 7 healthy fertile women and 20 oocytes from 9 patients with PCOS at the germinal vesicle (GV) stage, metaphase I (MI) stage, and metaphase II (MII) stage. We revealed the transcriptomic dynamics by weighted gene co-expression network analysis (WGCNA) and investigated the differences between stages using PCA and Deseq2 analyses to identify the differential expression genes (DEGs). Gene ontology (GO) was performed using clusterProfiler R package and Metascape. Our results indicated that specific gene modules were related to different stages of oocyte development using WGCNA. Functional enrichment analysis and gene co-expression network analysis found significant enrichment of the mitochondrial regulation genes at the GV stage. PCA (principal component analysis) and differential gene expression analysis suggested that GV was significantly different from the MI and MII stages between the two groups. Further analysis demonstrated that the upregulated differentially expressed genes at the GV stage of patients with PCOS mainly related to mitochondrial function, such as COX6B1, COX8A, COX4l1 , and NDUFB9. Meanwhile, these genes tended to be activated at the MII stage in healthy cells, suggesting that some mitochondrial functions may be prematurely activated at the GV stage of PCOS oocytes, whereas this process occurs at the MII stage in healthy oocytes. Collectively, our study showed that aberrant mitochondrial function at the GV stage may contribute to a decline in oocyte quality of PCOS patients. [ABSTRACT FROM AUTHOR]

This paper describes work performed in an attempt to bring more clarity regarding the differences in potential corrosive effect between sulfides and disulfides at specified reaction conditions as defined in ASTM 1275 B. SEM/EDS was used to further evaluate testing results. The results show that Dibenzyl disulfide (DBDS) has a strong tendency to form copper sulfides while Dibenzyl sulfide (DBS) display no tendency at all to form copper sulfides in the virgin insulating oils, but the reactivity of DBS increase if in the thermally degraded oils. In addition, the insulating paper decrease the oxidation of the copper surface due to that oxygen is adsorbed and consumed when migrating through the cellulose phase. With the improvement of surface smoothness, the specific surface area accessible for reactions will decrease and consequently it is more difficult for the copper to be corroded by DBDS. [ABSTRACT FROM AUTHOR]

In humans, the maternal endometrium participates in the physical and physiological interaction with the blastocyst to begin implantation. A bidirectional crosstalk is critical for normal implantation and then a successful pregnancy. While several studies have used animal models or cell lines to study this step, little knowledge was acquired to address the role of endometrial cells in humans. Here, we analyzed single-cell sequencing data from a previous study including 24 non-coculture endometrial stromal cells (EmSCs) and 57 EmSCs after coculture with embryos. We further explored the transcriptomic changes in EmSCs and their interactions with trophoblast cells after coculture. Differentially expressed gene (DEG) analysis showed 1783 upregulated genes and 569 downregulated genes in the cocultured embryos. Weight gene coexpression network and gene ontology analysis of these DEGs showed a higher expression of RAMP1, LTBP1, and LRP1 in EmSCs after coculture, indicating the enrichment of biological processes in blood vessel development and female pregnancy. These data imply that EmSCs start blood vessel development at the implantation stage. Compared with endometrium data in vivo at the implantation window, key pathways including epithelial cell development and oxygen response were involved at this stage. Further analysis using CellphoneDB shed light on the interactions between EmSCs and embryonic trophoblasts, suggesting the important role of integrins and fibroblast growth factor pathways during implantation. Taken together, our work reveals the synchronization signaling and pathways happening at the implantation stage involving the acquisition of receptivity in EmSCs and the interaction between EmSCs and trophoblast cells. [ABSTRACT FROM AUTHOR]

The analyses for temporal variation pollution sources of Qijiawan treatment pond show that in non-livestock and poultry breeding area, total phosphorus (TP) pollution mainly happens during the period from June to August with both farmland and domestic sewage as the main pollution source and during the Spring Festival with domestic sewage as the source. Total nitrogen (TN) pollution mainly occurs during the rainy season from July to September taking domestic sewage and nitrogen fertilizer as main sources, besides the Spring Festival with domestic sewage as the main pollution source. As for ammonia nitrogen pollution, it is similar to TN pollution, and domestic sewage is the first pollution source. The emphasis of pollution control should be put on the phosphorus especially particle phosphorus (PP) in the domestic sewage and dissolved organic nitrogen (DON) in the sewage and farmland fertilizer. In addition, nitrate‑nitrogen (NO 3 −-N) is mainly from the impervious land surface and transfers into the treatment pond with rainfall. COD into the pond in the rainy season was greater than that in the dry season, but anyhow, it firstly comes from domestic sewage, especially in the Spring Festival. Research on the purification performance of Myriophyllum spicatum treatment pond shows that it is relatively difficult for Myriophyllum spicatum to absorb COD and TN with DON as the main form, but it is very easy to absorb TP and NH 3 -N before October. Therefore, Myriophyllum spicatum should be harvested in this month in order to realize effective removal. The removal of COD and TN mainly depends on microbial degradation, and so it should be enhanced in order to improve water quality of treatment pond. Unlabelled Image [ABSTRACT FROM AUTHOR]

There is an increasing need for rapid and on-site detection of emerging drugs of abuse. In this work, we developed a method using a miniature dual-LIT (linear ion trap) mass spectrometer recently developed with comprehensive tandem mass spectrometry analysis capability, for qualitative and quantitative analysis of multiple drugs of abuse. Paper-capillary spray cartridges were used with related workflow established to simplify overall analysis procedure. Quantitation of ketamine and methamphetamine was achieved by beam-type collision-induced dissociation on the miniature dual-LIT mass spectrometer and a linear concentration range of 100–5000 ng/mL was obtained. The system has been applied in analysis of real urine samples from individuals addicted to morphine and methamphetamine use. The changes of the ratio of cocaine to its metabolite benzoylecgonine were also explored to estimate the time of cocaine intaking. Image 1 • Miniature dual-LIT mass spectrometry system was developed for quantitative analysis. • On-site testing of drugs of abuse in urine samples. • Testing of real urine samples from morphine and methamphetamine users. • Exploration of the time of cocaine intaking by the changes of cocaine/benzoylecgonine ratios in urine. [ABSTRACT FROM AUTHOR]

Recurrent implantation failure (RIF) associated with impaired endometrial receptivity and other factors. Disease-specific therapy has yet to be developed due to the lack of understanding of underlying mechanism(s). Herein we investigated the key factors of endometrial receptivity in RIF patients by transcriptomic sequencing. In vitro cellular model was used to delineate the molecular mechanism of key factors on proliferation, invasion and migration of trophoblast cells. SEMA4D was identified as the key factors of endometrial receptivity with significantly lower expression in the mid-secretory endometrium of RIF patients compared with those from normal fertile women. The binding of SEMA4D to its receptor Plexin-B1 on human trophoblast cells HTR-8/SVneo resulted in the activation of Met/PI3K/Akt signaling, which promotes trophoblast cell invasion and migration by enhancing MMP-2 expression. Moreover, the effect of SEMA4D on HTR-8/SVneo could be blocked by knocking down Met with specific siRNA or treating with LY294002. Collectively, our data indicate that decreased expression of SEMA4D in endometrium impair the process of trophoblast invasion and migration through Met/PI3K/Akt pathway, which provides insights into this essential physiological process in the development of RIF. • SEMA4D is a potential novel factor of the mid-luteal endometrium in the RIF patients. • SEMA4D promoted HTR-8/SVneo cells invasion and migration via Met/PI3K/Akt pathway. • SEMA4D promoted MMP-2 expression in HTR-8/SVneo cells. • Knockdown of Met could block SEMA4D mediated effects on trophoblast behavior. • Inhibition of the PI3K/Akt pathway abrogated SEMA4D mediated effects on trophoblast behavior. [ABSTRACT FROM AUTHOR]

Highlights: [•] TALENickases were described for the first time. [•] TALENickases stimulated targeted gene addition remarkably. [•] TALENickases were even more effective than TALENs at the rDNA locus. [ABSTRACT FROM AUTHOR]