23 results on '"Xiaohong Duan"'
Search Results
2. Characterization and mitigation of artifacts derived from NGS library preparation due to structure-specific sequences in the human genome
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HuiJuan Chen, YiRan Zhang, Bing Wang, Rui Liao, XiaoHong Duan, ChunYan Yang, Jing Chen, YanTong Hao, YingShuang Shu, LiLi Cai, Xue Leng, Nian-Song Qian, DaWei Sun, Beifang Niu, and Qiming Zhou
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Next generation sequencing ,Hybridization capture-based target NGS ,Sonication fragmentation ,Enzymatic fragmentation ,Sequencing errors ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Hybridization capture-based targeted next generation sequencing (NGS) is gaining importance in routine cancer clinical practice. DNA library preparation is a fundamental step to produce high-quality sequencing data. Numerous unexpected, low variant allele frequency calls were observed in libraries using sonication fragmentation and enzymatic fragmentation. In this study, we investigated the characteristics of the artifact reads induced by sonication and enzymatic fragmentation. We also developed a bioinformatic algorithm to filter these sequencing errors. Results We used pairwise comparisons of somatic single nucleotide variants (SNVs) and insertions and deletions (indels) of the same tumor DNA samples prepared using both ultrasonic and enzymatic fragmentation protocols. Our analysis revealed that the number of artifact variants was significantly greater in the samples generated using enzymatic fragmentation than using sonication. Most of the artifacts derived from the sonication-treated libraries were chimeric artifact reads containing both cis- and trans-inverted repeat sequences of the genomic DNA. In contrast, chimeric artifact reads of endonuclease-treated libraries contained palindromic sequences with mismatched bases. Based on these distinctive features, we proposed a mechanistic hypothesis model, PDSM (pairing of partial single strands derived from a similar molecule), by which these sequencing errors derive from ultrasonication and enzymatic fragmentation library preparation. We developed a bioinformatic algorithm to generate a custom mutation “blacklist” in the BED region to reduce errors in downstream analyses. Conclusions We first proposed a mechanistic hypothesis model (PDSM) of sequencing errors caused by specific structures of inverted repeat sequences and palindromic sequences in the natural genome. This new hypothesis predicts the existence of chimeric reads that could not be explained by previous models, and provides a new direction for further improving NGS analysis accuracy. A bioinformatic algorithm, ArtifactsFinder, was developed and used to reduce the sequencing errors in libraries produced using sonication and enzymatic fragmentation.
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- 2024
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3. Multifunctionality of temperate alley-cropping agroforestry outperforms open cropland and grassland
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Edzo Veldkamp, Marcus Schmidt, Christian Markwitz, Lukas Beule, René Beuschel, Andrea Biertümpfel, Xenia Bischel, Xiaohong Duan, Rowena Gerjets, Leonie Göbel, Rüdiger Graß, Victor Guerra, Florian Heinlein, Martin Komainda, Maren Langhof, Jie Luo, Martin Potthoff, Justus G. V. van Ramshorst, Carolin Rudolf, Diana-Maria Seserman, Guodong Shao, Lukas Siebicke, Nikolai Svoboda, Anita Swieter, Andrea Carminati, Dirk Freese, Torsten Graf, Jörg M. Greef, Johannes Isselstein, Martin Jansen, Petr Karlovsky, Alexander Knohl, Norbert Lamersdorf, Eckart Priesack, Christine Wachendorf, Michael Wachendorf, and Marife D. Corre
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Geology ,QE1-996.5 ,Environmental sciences ,GE1-350 - Abstract
Alley-cropping agroforestry enhances carbon sequestration compared to open cropland and open grassland, and improves soil biological habitat and erosion resistance relative to open cropland, based on measured ecosystem functions at five sites in Germany.
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- 2023
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4. Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway
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Zanyan Zhao, Xiangpu Wang, Yu Ma, and Xiaohong Duan
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osteoporosis ,Atp6v1h ,Fos-Jun-Src-Integrin pathway ,tail suspension model ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The microgravity conditions in outer space are widely acknowledged to induce significant bone loss. Recent studies have implicated the close relationship between Atp6v1h gene and bone loss. Despite this, the role of Atp6v1h in bone remodeling and its molecular mechanisms in microgravity have not been fully elucidated. To address this, we used a mouse tail suspension model to simulate microgravity. We categorized both wild-type and Atp6v1h knockout (Atp6v1h+/-) mice into two groups: regular feeding and tail-suspension feeding, ensuring uniform feeding conditions across all cohorts. Analysis via micro-CT scanning, hematoxylin-eosin staining, and tartrate-resistant acid phosphatase assays indicated that wild-type mice underwent bone loss under simulated microgravity. Atp6v1h+/- mice exhibited bone loss due to Atp6v1h deficiency but did not present aggravated bone loss under the same simulated microgravity. Transcriptomic sequencing revealed the upregulation of genes, such as Fos, Src, Jun, and various integrin subunits in the context of simulated microgravity and Atp6v1h knockout. Real-time quantitative polymerase chain reaction (RT-qPCR) further validated the modulation of downstream osteoclast-related genes in response to interactions with ATP6V1H overexpression cell lines. Co-immunoprecipitation indicated potential interactions between ATP6V1H and integrin beta 1, beta 3, beta 5, alpha 2b, and alpha 5. Our results indicate that Atp6v1h level influences bone loss in simulated microgravity by modulating the Fos-Jun-Src-Integrin pathway, which, in turn, affects osteoclast activity and bone resorption, with implications for osteoporosis. Therefore, modulating Atp6v1h expression could mitigate bone loss in microgravity conditions. This study elucidates the molecular mechanism of Atp6v1h’s role in osteoporosis and positions it as a potential therapeutic target against environmental bone loss. These findings open new possibilities for the treatment of multifactorial osteoporosis.
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- 2024
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5. K205R specific nanobody-horseradish peroxidase fusions as reagents of competitive ELISA to detect African swine fever virus serum antibodies
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Angke Zhang, Shuya Wu, Xiaohong Duan, Huijun Zhao, Haoxin Dong, Jiahui Ren, Mingfang Zhang, Jiaji Li, Hong Duan, and Gaiping Zhang
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Nanobody-HRP ,ASFV ,K205R ,cELISA ,Antibody ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background African swine fever virus (ASFV) is a highly contagious hemorrhagic disease and often lethal, which has significant economic consequences for the swine industry. Due to lacking of commercial vaccine, the prevention and control of ASF largely depend on early large-scale detection and screening. So far, the commercial ELISA kits have a long operation time and are expensive, making it difficult to achieve large-scale clinical applications. Nanobodies are single-domain antibodies produced by camelid animals, and have unique advantages such as smaller molecular weight, easy genetic engineering modification and low-costing of mass production, thus exhibiting good application prospects. Results The present study developed a new method for detection of ASFV specific antibodies using nanobody-horseradish peroxidase (Nb-HRP) fusion proteins as probe. By using camel immunization, phage library construction and phage display technology, five nanobodies against K205R protein were screened. Then, Nb-HRP fusion proteins were produced using genetic modification technology. Based on the Nb-HRP fusion protein as specific antibodies against K205R protein, a new type of cELISA was established to detect ASFV antibodies in pig serum. The cut-off value of the cELISA was 34.8%, and its sensitivity, specificity, and reproducibility were good. Furthermore, the developed cELISA exhibited 99.3% agreement rate with the commercial available ELISA kit (kappa value = 0.98). Conclusions The developed cELISA method has the advantages of simple operation, rapid and low-costing, and can be used for monitoring of ASFV infection in pigs, thus providing a new method for the prevention and control of ASF.
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- 2022
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6. Potential Theranostic Roles of SLC4 Molecules in Human Diseases
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Jingwen Zhong, Jing Dong, Wenyan Ruan, and Xiaohong Duan
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solute carrier family 4 ,transporter ,physiology ,genetic disease ,therapy ,diagnose ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The solute carrier family 4 (SLC4) is an important protein responsible for the transport of various ions across the cell membrane and mediating diverse physiological functions, such as the ion transporting function, protein-to-protein interactions, and molecular transduction. The deficiencies in SLC4 molecules may cause multisystem disease involving, particularly, the respiratory system, digestive, urinary, endocrine, hematopoietic, and central nervous systems. Currently, there are no effective strategies to treat these diseases. SLC4 proteins are also found to contribute to tumorigenesis and development, and some of them are regarded as therapeutic targets in quite a few clinical trials. This indicates that SLC4 proteins have potential clinical prospects. In view of their functional characteristics, there is a critical need to review the specific functions of bicarbonate transporters, their related diseases, and the involved pathological mechanisms. We summarize the diseases caused by the mutations in SLC4 family genes and briefly introduce the clinical manifestations of these diseases as well as the current treatment strategies. Additionally, we illustrate their roles in terms of the physiology and pathogenesis that has been currently researched, which might be the future therapeutic and diagnostic targets of diseases and a new direction for drug research and development.
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- 2023
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7. Famotidine promotes inflammation by triggering cell pyroptosis in gastric cancer cells
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Jin Huang, Pingsheng Fan, Miao Liu, Chengtao Weng, Gaofei Fan, Tengyue Zhang, Xiaohong Duan, Yang Wu, Lili Tang, Guohong Yang, and Yabei Liu
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Famotidine ,Cell pyroptosis ,GSDME, gastric cancer cells ,Therapeutics. Pharmacology ,RM1-950 ,Toxicology. Poisons ,RA1190-1270 - Abstract
Abstract Background Cell pyroptosis has been characterized by cell swelling and pro-inflammatory factors release to aggravate inflammatory reaction., such as interlukin-1 beta (IL-1β) and interlukin18 (IL-18). However, the function of famotidine, an antagonist of histamine H2-receptor antagonists, in cell pyroptosis remained unknown. Methods Real-time quantitative PCR (qPCR), western blotting (WB), LDH release assay and enzyme linked immunosorbent assay (Elisa) combined with inhibitor were performed to analyze the effect of famotidine on cell pyroptosis-related gene expression. Results In this study, we found that famotidine (300 μm) treatment led to a phenomenon of cell pyroptosis as confirmed by LDH assay. Further results showed that famotidine triggered cell pyroptosis in gastric cancer cells by activation of NLPR3 inflammasomes including ASC, Caspase-1 and NLRP, leading to enhanced IL-18, not IL-1β, mature and secretion. What’s more, the results also showed GSDME, not GSDMD, was increased in response to famotidine stimulation in BGC823 and AGS cells. Mechanically, phosphorylation of ERK1/2 was drastically enhanced in present with famotidine treatment, while inhibition of ERK1/2 activity by U0126 could reverse the promotion of famotidine in IL-18 secretion. Conclusion These findings revealed a novel role of famotidine in cell pyroptosis in patients with gastric cancer, a comprehensive consideration is needed in treatment of gastric cancer.
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- 2021
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8. Exosomal microRNA panel as a diagnostic biomarker in patients with hepatocellular carcinoma
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Jingwen Yang, Weiwei Dong, He Zhang, Huixia Zhao, Zhiyan Zeng, Fengyun Zhang, Qiuwen Li, Xiaohong Duan, Yanyan Hu, and Wenhua Xiao
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exosome ,plasma ,microRNA ,diagnostic biomarker ,hepatocellular carcinoma ,Biology (General) ,QH301-705.5 - Abstract
Background: Diagnostic tools for hepatocellular carcinoma (HCC) are critical for patient treatment and prognosis. Thus, this study explored the diagnostic value of the exosomal microRNA panel for HCC.Methods: Expression profiles of microRNAs in exosomes and plasma of HCC and control groups were assessed using microRNA microarray analysis. Reverse transcription-quantitative PCR was applied to evaluate the expression of candidate microRNAs in blood samples from 50 HCC patients, 50 hepatic cirrhosis patients, and 50 healthy subjects. The area calculated the diagnostic accuracy of the microRNAs and microRNA panel under the receiver operating characteristic curve (AUC).Results: MicroRNA microarray analysis revealed that there were more differentially expressed microRNAs in the exosome HCC group than plasma HCC group. Among the 43 differentially expressed microRNAs contained in both exosomes and plasma, we finally decided to testify the expression and diagnostic significance of microRNA-26a, microRNA-29c, and microRNA-199a. The results indicated that expression of the microRNA-26a, microRNA-29c, and microRNA-199a in both exosomes and plasma was significantly lower in HCC patients compared with hepatic cirrhosis and healthy group. Interestingly, exosomal microRNAs were substantially more accurate in diagnosing HCC than microRNAs and alpha-fetoprotein in plasma. Moreover, the exosomal microRNA panel containing microRNA-26a, microRNA-29c, and microRNA-199a showed high accuracy in discriminating HCC from healthy (AUC = 0.994; sensitivity 100%; specificity 96%) and hepatic cirrhosis group (AUC = 0.965; sensitivity 92%; specificity 90%).Conclusion: This study revealed that the exosomal microRNA panel has high accuracy in diagnosing HCC and has important clinical significance.
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- 2022
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9. Same soil, different climate: Crop model intercomparison on translocated lysimeters
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Jannis Groh, Efstathios Diamantopoulos, Xiaohong Duan, Frank Ewert, Florian Heinlein, Michael Herbst, Maja Holbak, Bahareh Kamali, Kurt‐Christian Kersebaum, Matthias Kuhnert, Claas Nendel, Eckart Priesack, Jörg Steidl, Michael Sommer, Thomas Pütz, Jan Vanderborght, Harry Vereecken, Evelyn Wallor, Tobias K. D. Weber, Martin Wegehenkel, Lutz Weihermüller, and Horst H. Gerke
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Environmental sciences ,GE1-350 ,Geology ,QE1-996.5 - Abstract
Abstract Crop model intercomparison studies have mostly focused on the assessment of predictive capabilities for crop development using weather and basic soil data from the same location. Still challenging is the model performance when considering complex interrelations between soil and crop dynamics under a changing climate. The objective of this study was to test the agronomic crop and environmental flux‐related performance of a set of crop models. The aim was to predict weighing lysimeter‐based crop (i.e., agronomic) and water‐related flux or state data (i.e., environmental) obtained for the same soil monoliths that were taken from their original environment and translocated to regions with different climatic conditions, after model calibration at the original site. Eleven models were deployed in the study. The lysimeter data (2014–2018) were from the Dedelow (Dd), Bad Lauchstädt (BL), and Selhausen (Se) sites of the TERENO (TERrestrial ENvironmental Observatories) SOILCan network. Soil monoliths from Dd were transferred to the drier and warmer BL site and the wetter and warmer Se site, which allowed a comparison of similar soil and crop under varying climatic conditions. The model parameters were calibrated using an identical set of crop‐ and soil‐related data from Dd. Environmental fluxes and crop growth of Dd soil were predicted for conditions at BL and Se sites using the calibrated models. The comparison of predicted and measured data of Dd lysimeters at BL and Se revealed differences among models. At site BL, the crop models predicted agronomic and environmental components similarly well. Model performance values indicate that the environmental components at site Se were better predicted than agronomic ones. The multi‐model mean was for most observations the better predictor compared with those of individual models. For Se site conditions, crop models failed to predict site‐specific crop development indicating that climatic conditions (i.e., heat stress) were outside the range of variation in the data sets considered for model calibration. For improving predictive ability of crop models (i.e., productivity and fluxes), more attention should be paid to soil‐related data (i.e., water fluxes and system states) when simulating soil–crop–climate interrelations in changing climatic conditions.
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- 2022
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10. Molecular Features in Lymphatic Metastases Reflect the Metastasis Mechanism of Lymph Nodes With Non-Small-Cell Lung Cancers
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Nannan Guo, Yuanyuan Chen, Zhongying Jing, Siyao Liu, Junyan Su, Ruilin Li, Xiaohong Duan, Zhigong Chen, Ping Chen, Rongjiang Yin, Shaojun Li, and Jian Tang
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NSCLC ,lymphatic metastasis ,molecular features ,ATR ,TET2 ,Biotechnology ,TP248.13-248.65 - Abstract
Lymphatic metastasis influences clinical treatment and prognosis of patients with non-small-cell lung cancer (NSCLC). There is an urgency to understand the molecular features and mechanisms of lymph node metastasis. We analyzed the molecular features on pairs of the primary tumor and lymphatic metastasis tissue samples from 15 NSCLC patients using targeted next-generation sequencing. The potential metastasis-related genes were screened from our cohort based on cancer cell fraction. After filtering with gene functions, candidate metastasis-related events were validated in the MSK cohort with Fisher’s exact test. The molecular signature and tumor mutational burden were similar in paired samples, and the average mutational concordance was 42.0% ± 28.9%. Its metastatic mechanism is potentially a linear progression based on the metastatic seeding theory. Furthermore, mutated ataxia telangiectasia mutated and Rad3-related (ATR) and tet methylcytosine dioxygenase 2 (TET2) genes were significantly enriched in lymphatic metastases (p ≤ 0.05). Alterations in these two genes could be considered metastasis-related driving events. Mutated ATR and TET2 might play an active role in the metastasis of lymph nodes with NSCLC. More case enrollment and long-term follow-up will further verify the clinical significance of these two genes.
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- 2022
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11. Molecular Mechanisms of Craniofacial and Dental Abnormalities in Osteopetrosis
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Yu Ma, Yali Xu, Yanli Zhang, and Xiaohong Duan
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osteopetrosis ,osteoclast ,tooth ,craniofacial bone ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Osteopetrosis is a group of genetic bone disorders characterized by increased bone density and defective bone resorption. Osteopetrosis presents a series of clinical manifestations, including craniofacial deformities and dental problems. However, few previous reports have focused on the features of craniofacial and dental problems in osteopetrosis. In this review, we go through the clinical features, types, and related pathogenic genes of osteopetrosis. Then we summarize and describe the characteristics of craniofacial and dental abnormalities in osteopetrosis that have been published in PubMed from 1965 to the present. We found that all 13 types of osteopetrosis have craniomaxillofacial and dental phenotypes. The main pathogenic genes, such as chloride channel 7 gene (CLCN7), T cell immune regulator 1 (TCIRG1), osteopetrosis-associated transmembrane protein 1 (OSTM1), pleckstrin homology domain-containing protein family member 1 (PLEKHM1), and carbonic anhydrase II (CA2), and their molecular mechanisms involved in craniofacial and dental phenotypes, are discussed. We conclude that the telltale craniofacial and dental abnormalities are important for dentists and other clinicians in the diagnosis of osteopetrosis and other genetic bone diseases.
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- 2023
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12. DIVIS: Integrated and Customizable Pipeline for Cancer Genome Sequencing Analysis and Interpretation
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Xiaoyu He, Yu Zhang, Danyang Yuan, Xinyin Han, Jiayin He, Xiaohong Duan, Siyao Liu, Xintong Wang, and Beifang Niu
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variants detection ,customization ,workflow ,next-generation sequencing ,cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Next-generation sequencing (NGS) has drastically enhanced human cancer research, but diverse sequencing strategies, complicated open-source software, and the identification of massive numbers of mutations have limited the clinical application of NGS. Here, we first presented GPyFlow, a lightweight tool that flexibly customizes, executes, and shares workflows. We then introduced DIVIS, a customizable pipeline based on GPyFlow that integrates read preprocessing, alignment, variant detection, and annotation of whole-genome sequencing, whole-exome sequencing, and gene-panel sequencing. By default, DIVIS screens variants from multiple callers and generates a standard variant-detection format list containing caller evidence for each sample, which is compatible with advanced analyses. Lastly, DIVIS generates a statistical report, including command lines, parameters, quality-control indicators, and mutation summary. DIVIS substantially facilitates complex cancer genome sequencing analyses by means of a single powerful and easy-to-use command. The DIVIS code is freely available at https://github.com/niu-lab/DIVIS, and the docker image can be downloaded from https://hub.docker.com/repository/docker/sunshinerain/divis.
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- 2021
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13. A Novel 90-kbp Deletion of RUNX2 Associated with Cleidocranial Dysplasia
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Yanli Zhang and Xiaohong Duan
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cleidocranial dysplasia ,large fragment deletion ,RUNX2 ,novel mutation ,Genetics ,QH426-470 - Abstract
Cleidocranial dysplasia (CCD) is a rare autosomal dominant skeletal dysplasia caused by runt-related transcription factor 2 (RUNX2) mutations. In addition to the regular missense, small or large fragment deletions are the common mutation types of RUNX2. This study aimed to find the rules of deletions in RUNX2. The clinical information of one Chinese CCD family was collected. Genomic DNA was extracted for whole-exome sequencing (WES). Bioinformatics analyzed the pathogenicity of the variants. Polymerase chain reaction (PCR) and Sanger sequencing were carried out using specific primers. RT-PCR and Q-PCR were also used to detect the mRNA level of RUNX2. The CCD studies related with deletions in RUNX2 from 1999 to 2021 from HGMD and PubMed were collected and analyzed for the relationship between the phenotypes and the length of deleted fragments. The proband presented typical CCD features, including delayed closure of cranial sutures, clavicle dysplasia, abnormal teeth. WES, PCR with specific primers and Sanger sequencing revealed a novel heterozygous 90-kbp deletion in RUNX2 (NG_008020.2 g.103671~193943), which caused a substitution (p.Asn183Ile) and premature termination (p.Asp184*). In addition, the mRNA expression of RUNX2 was decreased by 75.5% in the proband. Herein, 31 types of deletions varying from 2 bp to 800 kbp or covering the whole gene of RUNX2 were compared and the significant phenotypic difference was not found among these deletions. The CCD phenotypes were related with the final effects of RUNX2 mutation instead of the length of deletion. WES has the defects in identifying large indels, and direct PCR with specific primers and Sanger sequencing could make up for the shortcoming.
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- 2022
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14. Crop growth and soil water fluxes at erosion‐affected arable sites: Using weighing lysimeter data for model intercomparison
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Jannis Groh, Efstathios Diamantopoulos, Xiaohong Duan, Frank Ewert, Michael Herbst, Maja Holbak, Bahareh Kamali, Kurt‐Christian Kersebaum, Matthias Kuhnert, Gunnar Lischeid, Claas Nendel, Eckart Priesack, Jörg Steidl, Michael Sommer, Thomas Pütz, Harry Vereecken, Evelyn Wallor, Tobias K.D. Weber, Martin Wegehenkel, Lutz Weihermüller, and Horst H. Gerke
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Environmental sciences ,GE1-350 ,Geology ,QE1-996.5 - Abstract
Abstract Agroecosystem models need to reliably simulate all biophysical processes that control crop growth, particularly the soil water fluxes and nutrient dynamics. As a result of the erosion history, truncated and colluvial soil profiles coexist in arable fields. The erosion‐affected field‐scale soil spatial heterogeneity may limit agroecosystem model predictions. The objective was to identify the variation in the importance of soil properties and soil profile modifications in agroecosystem models for both agronomic and environmental performance. Four lysimeters with different soil types were used that cover the range of soil variability in an erosion‐affected hummocky agricultural landscape. Twelve models were calibrated on crop phenological stages, and model performance was tested against observed grain yield, aboveground biomass, leaf area index, actual evapotranspiration, drainage, and soil water content. Despite considering identical input data, the predictive capability among models was highly diverse. Neither a single crop model nor the multi‐model mean was able to capture the observed differences between the four soil profiles in agronomic and environmental variables. The model's sensitivity to soil‐related parameters was apparently limited and dependent on model structure and parameterization. Information on phenology alone seemed insufficient to calibrate crop models. The results demonstrated model‐specific differences in the impact of soil variability and suggested that soil matters in predictive agroecosystem models. Soil processes need to receive greater attention in field‐scale agroecosystem modeling; high‐precision weighable lysimeters can provide valuable data for improving the description of soil–vegetation–atmosphere process in the tested models.
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- 2020
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15. Surface Pollen Distribution from Alpine Vegetation in Eastern Tibet, China
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Yun Zhang, Zhaochen Kong, Zhenjing Yang, Li Wang, and Xiaohong Duan
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Medicine ,Science - Abstract
Abstract We explore the relationship between modern pollen spectra and vegetation patterns in the Eastern Tibet, China in order to provide information on the representation of pollen taxa and improve the general knowledge of vertical pollen transport. Forty-two modern pollen samples collected in surface soil along two altitudinal transects allowed conclusions on vertical pollen dispersal from the alpine region of Dingqing County, Changdu district in Tibet. Discriminant analyses and detrended correspondence analysis (DCA) of 24 pollen taxa were used to further discuss the difference of modern pollen spectra in these alpine vegetation zones. The surface pollen assemblage is divided into three pollen zones, such as subalpine shrub meadow, montane coniferous forest and shrub steppe with sparse trees. Altitude and precipitation are two primary factors contributing to changes in surface pollen assemblage from alpine vegetation in the eastern Tibet. Large amounts of spruce pollen at higher elevations above the timberline might be introduced from lower elevations by upslope winds. Therefore, the interpretation of spruce pollen in the fossil record must take into account long distance upward wind transport. Moreover, the destruction of coniferous forest in the study area is well illustrated in the modern pollen rain.
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- 2017
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16. ATP6V1H Deficiency Impairs Bone Development through Activation of MMP9 and MMP13.
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Yihan Zhang, Haigen Huang, Gexin Zhao, Tadafumi Yokoyama, Hugo Vega, Yan Huang, Raman Sood, Kevin Bishop, Valerie Maduro, John Accardi, Camilo Toro, Cornelius F Boerkoel, Karen Lyons, William A Gahl, Xiaohong Duan, May Christine V Malicdan, and Shuo Lin
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Genetics ,QH426-470 - Abstract
ATP6V1H is a component of a large protein complex with vacuolar ATPase (V-ATPase) activity. We identified two generations of individuals in which short stature and osteoporosis co-segregated with a mutation in ATP6V1H. Since V-ATPases are highly conserved between human and zebrafish, we generated loss-of-function mutants in atp6v1h in zebrafish through CRISPR/Cas9-mediated gene knockout. Homozygous mutant atp6v1h zebrafish exhibited a severe reduction in the number of mature calcified bone cells and a dramatic increase in the expression of mmp9 and mmp13. Heterozygous adults showed curved vertebra that lack calcified centrum structure and reduced bone mass and density. Treatment of mutant embryos with small molecule inhibitors of MMP9 and MMP13 significantly restored bone mass in the atp6v1h mutants. These studies have uncovered a new, ATP6V1H-mediated pathway that regulates bone formation, and defines a new mechanism of disease that leads to bone loss. We propose that MMP9/MMP13 could be therapeutic targets for patients with this rare genetic disease.
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- 2017
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17. Correction: ATP6V1H Deficiency Impairs Bone Development through Activation of MMP9 and MMP13.
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Yihan Zhang, Haigen Huang, Gexin Zhao, Tadafumi Yokoyama, Hugo Vega, Yan Huang, Raman Sood, Kevin Bishop, Valerie Maduro, John Accardi, Camilo Toro, Cornelius F Boerkoel, Karen Lyons, William A Gahl, Xiaohong Duan, May Christine V Malicdan, and Shuo Lin
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Genetics ,QH426-470 - Abstract
[This corrects the article DOI: 10.1371/journal.pgen.1006481.].
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- 2017
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18. Dynamic Path Planning of Emergency Vehicles Based on Travel Time Prediction
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Jiandong Zhao, Yujie Guo, and Xiaohong Duan
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Transportation engineering ,TA1001-1280 ,Transportation and communications ,HE1-9990 - Abstract
The dynamic paths planning problem of emergency vehicles is usually constrained by the factors including time efficiency, resources requirement, and reliability of the road network. Therefore, a two-stage model of dynamic paths planning of emergency vehicles is built with the goal of the shortest travel time and the minimum degree of traffic congestion. Firstly, according to the dynamic characteristics of road network traffic, a polyline-shaped speed function is constructed. And then, based on the real-time and historical data of travel speed, a new kernel clustering algorithm based on shuffled frog leaping algorithm is designed to predict the travel time. Secondly, combined with the expected travel time, the traffic congestion index is defined to measure the reliability of the route. Thirdly, aimed at the problem of solving two-stage target model, a two-stage shortest path algorithm is proposed, which is composed of K-paths algorithm and shuffled frog leaping algorithm. Finally, based on the data of floating vehicles of expressway in Beijing, a simulation case is used to verify the above methods. The results show that the optimization path algorithm meets the needs of the multiple constraints.
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- 2017
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19. Herbicide weed control increases nutrient leaching as compared to mechanical weeding in a large-scale oil palm plantation.
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Formaglio, Greta, Veldkamp, Edzo, Xiaohong Duan, Tjoa, Aiyen, and Corre, Marife D.
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OIL palm ,WEED control ,EFFECT of herbicides on plants ,HERBICIDES ,LEACHING ,WEEDS ,HERBICIDE resistance - Abstract
Nutrient leaching in intensively managed oil palm plantations can diminish soil fertility and water quality. There is a need to reduce this environmental footprint without sacrificing yield. We quantified nutrient leaching in a large-scale oil palm plantation on Acrisol soil with factorial treatment combinations of two fertilization rates (260 N, 50 P, 220 K kg ha
-1 yr-1 as conventional practice, and 136 N, 17 P, 187 K kg ha-1 yr-1 , equal to harvest export, as reduced management) and two weeding methods (conventional herbicide, and mechanical weeding as reduced management). Each of the four treatment combinations was represented by a 2500 m² plot, replicated in four blocks. In each plot, soil-pore water was collected monthly at 1.5 m depth for one year in three management zones: palm circle, inter-row, and frond-stacked area. In the palm circle, nutrient leaching was low due to low solute concentrations and small drainage fluxes, resulting from large plant uptake. Conversely, in the inter-row, nitrate and aluminum leaching losses were high due to their high concentrations, large drainage fluxes, low plant uptake, and acidic pH. In the frond-stacked area, base cation leaching was high, presumably from frond litter decomposition, but N leaching was low. Mechanical weeding, even with conventional high fertilization rates, reduced leaching losses of all nutrients. Mechanical weeding with reduced fertilization had the lowest N and base cation leaching whereas its yield and economic gross margin remain comparable with the conventional management practices. Herbicide weed control decreased ground vegetation, and thereby reduced efficiency of soil nutrient retention. Our findings signified that mechanical weeding and reduced fertilization should be included in the Indonesian Ministry of Agriculture program for precision farming (e.g. variable rates with plantation age), particularly for large-scale plantations, and in the science-based policy recommendations, such as those endorsed by the Roundtable for Sustainable Palm Oil association. [ABSTRACT FROM AUTHOR]- Published
- 2020
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20. Correlation between genotype and phenotype in three families with Peutz-Jeghers Syndrome.
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YANLI ZHANG, YAO KE, XUENI ZHENG, QING LIU, and XIAOHONG DUAN
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STATISTICAL correlation ,GENOTYPES ,PHENOTYPES ,PEUTZ-Jeghers syndrome ,POLYMERASE chain reaction ,NUCLEOTIDE sequence ,CLONING ,GENETICS - Abstract
Peutz-Jeghers syndrome (PJS) is a hereditary disorder characterized by mucocutaneous pigmentations, gastrointestinal (GI) polyposis and an increased risk of certain malignancies. Little is known about the causative genes of PJS, or their association with the clinical phenotypes of PJS. The present study reports the results of clinical and genetic analysis of three Chinese families with PJS. In addition, the medical histories and clinical manifestations of these families were compared. DNA was collected from the blood samples of patients with PJS and controls. Serine/threonine kinase 11 (STK11), olfactory receptor family 4 subfamily C member 45 (OR4C45) and zonadhesin (ZAN) were amplified by polymerase chain reaction, and analyzed by sequencing and cloning. Two PJS-affected members of one family had a de novo single base deletion (NM_000455.4:c.842delC) in the STK11 gene, and their clinical presentations reflected the quantity of mutant STK11 copies in a dose-dependent manner. No pathogenic variants of OR4C45 or ZAN were found in the patients with PJS, although a new single nucleotide polymorphism (NM_003386.2:c.5768delG) of ZAN was identified. The results of the current study identified that a STK11 mutation dose-dependent genotype-phenotype relationship exists in patients with PJS. In addition, an early onset and high severity of oral pigmentations in PJS was indicative of serious GI phenotypes. These findings may aid the diagnosis and treatment of PJS. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
21. PeanutDB: an integrated bioinformatics web portal for Arachis hypogaea transcriptomics.
- Author
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Xiaohong Duan, Emily Schmidt, Pei Li, Lenox, Douglas, Liu, Lin, Changlong Shu, Jie Zhang, and Chun Liang
- Subjects
- *
BIOINFORMATICS , *GENETIC polymorphisms , *POPULATION genetics , *PEANUTS , *GENETIC transcription - Abstract
Background: The peanut (Arachis hypogaea) is an important crop cultivated worldwide for oil production and food sources. Its complex genetic architecture (e.g., the large and tetraploid genome possibly due to unique cross of wild diploid relatives and subsequent chromosome duplication: 2n = 4x = 40, AABB, 2800 Mb) presents a major challenge for its genome sequencing and makes it a less-studied crop. Without a doubt, transcriptome sequencing is the most effective way to harness the genome structure and gene expression dynamics of this non-model species that has a limited genomic resource. Description: With the development of next generation sequencing technologies such as 454 pyro-sequencing and Illumina sequencing by synthesis, the transcriptomics data of peanut is rapidly accumulated in both the public databases and private sectors. Integrating 187,636 Sanger reads (103,685,419 bases), 1,165,168 Roche 454 reads (333,862,593 bases) and 57,135,995 Illumina reads (4,073,740,115 bases), we generated the first release of our peanut transcriptome assembly that contains 32,619 contigs. We provided EC, KEGG and GO functional annotations to these contigs and detected SSRs, SNPs and other genetic polymorphisms for each contig. Based on both open-source and our in-house tools, PeanutDB presents many seamlessly integrated web interfaces that allow users to search, filter, navigate and visualize easily the whole transcript assembly, its annotations and detected polymorphisms and simple sequence repeats. For each contig, sequence alignment is presented in both bird's-eye view and nucleotide level resolution, with colorfully highlighted regions of mismatches, indels and repeats that facilitate close examination of assembly quality, genetic polymorphisms, sequence repeats and/or sequencing errors. Conclusion: As a public genomic database that integrates peanut transcriptome data from different sources, PeanutDB (http://bioinfolab.muohio.edu/txid3818v1) provides the Peanut research community with an easy-to-use web portal that will definitely facilitate genomics research and molecular breeding in this less-studied crop. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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22. Clinical and animal research findings in pycnodysostosis and gene mutations of cathepsin K from 1996 to 2011.
- Author
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Yang Xue, Tao Cai, Songtao Shi, Weiguang Wang, Yanli Zhang, Tianqiu Mao, Xiaohong Duan, Xue, Yang, Cai, Tao, Shi, Songtao, Wang, Weiguang, Zhang, Yanli, Mao, Tianqiu, and Duan, Xiaohong
- Subjects
GENETIC mutation ,PAPAIN ,BONE diseases ,COLLAGEN ,PHENOTYPES - Abstract
Cathepsin K (CTSK) is a member of the papain-like cysteine protease family. Mutations in the CTSK gene cause a rare autosomal recessive bone disorder called pycnodysostosis (OMIM 265800). In order to follow the advances in the research about CTSK and pycnodysostosis, we performed a literature retrospective study of 159 pycnodysostosis patients reported since 1996 and focused on the genetic characteristics of CTSK mutations and/or the clinical phenotypes of pycnodysostosis. Thirty three different CTSK mutations have been found in 59 unrelated pycnodysostosis families. Of the 59 families, 37.29% are from Europe and 30.51% are from Asia. A total of 69.70% of the mutations were identified in the mature domain of CTSK, 24.24% in the proregion, and 6.06% in the preregion. The hot mutation spots are found in exons 6 and 7. CTSK mutations result in total loss or inactivity of the CTSK protein, which causes abnormal degradation of bone matrix proteins such as type I collagen. Skeletal abnormalities, including short stature, an increase in bone density with pathologic fractures, and open fontanels and sutures, are the typical phenotypes of pycnodysostosis. Research on Ctsk(-/-) mouse models was also reviewed here to elucidate the biological function of Ctsk and the mechanism of pycnodysostosis. New evidence suggests that Ctsk plays an important role in the immune system and may serve as a valid therapeutic target in the future treatment of pycnodysostosis. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
23. Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3).
- Author
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Zhihong Lin, Shi Jin, Xiaohong Duan, Tong Wang, Martini, Sabrina, Hulamm, Phuson, Boyoung Cha, Hubbar, Ann, Donowitz, Mark, and Guggin, Sandra E.
- Subjects
- *
ION channels , *MOBILE genetic elements , *CELL membranes , *PARATHYROID hormone , *ANTIHYPERTENSIVE agents - Abstract
ClC-5, a chloride/proton exchanger, is predominantly expressed and localized in subapical endosomes of the renal proximal tubule. Mutations of the CLCN5 gene cause Dent disease. The symptoms of Dent disease are replicated in Clcn5 knock-out mice. Absence of ClC-5 in mice is associated with reduced surface expression of NHE3 in proximal tubules. The molecular basis for this change is not fully understood. In this study, we investigated the mechanisms by which ClC-5 regulates trafficking of NHE3. Whether ClC-5-dependent endocytosis, exocytosis, or both contributed to the altered distribution of NHE3 was examined. First, NHE3 activity in proximal tubules of wild type (WT) and Clcn5 KO mice was determined by two-photon microscopy. Basal and dexamethasone-stimulated NHE3 activity of Clcn5 KO mice was decreased compared with that seen in WT mice, whereas the degree of inhibition of NHE3 activity by increasing cellular concentration of cAMP (forskolin) or Ca2+ (A23187) was not different in WT and Clcn5 KO mice. Second, NHE3-dependent absorption of HCO3-, measured by single tubule perfusion, was reduced in proximal tubules of Clcn5 KO mice. Third, by cell surface biotinylation, trafficking of NHE3 was examined in short hairpin RNA (shRNA) plasmid-transfected opossum kidney cells. Surface NHE3 was reduced in opossum kidney cells with reduced expression of ClC-5, whereas the total protein level of NHE3 did not change. Parathyroid hormone decreased NHE3 surface expression, but the extent of decrease and the rate of endocytosis observed in both scrambled and ClC-5 knockdown cells were not significantly different. However, the rates of basal and dexamethasone-stimulated exocytosis of NHE3 were attenuated in ClC-5 knockdown cells. These results show that ClC-5 plays an essential role in exocytosis of NHE3. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
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