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Since social and emotional development in early childhood is so significant for success later in life and the foundation of the capacity for learning, it is essential for caregivers to build these capacities in young children through everyday moments (Russell, Lee, Spieker, & Oxford, 2016; Turculet & Tulbure, 2014). Caregivers who provide the child with choices, discuss the child's experiences, and are sensitive to the child's emotional states promote the development of empathy and social and emotional competence (Roopnarine & Davidson, 2015). Caregivers who respond to the child's actions and emotions and talk about those of others provide those learning opportunities, which is especially significant for children with special needs (Shire, Gulsrud, & Kasari, 2016; Test, Cunningham, & Lee, 2010). These relationships are bidirectional, as the ways caregivers respond to the child change as a child grows from an infant through the preschool years, and how the child responds to caregivers and their developmental needs can influence the caregiver's response. An increase in intentionality on behalf of the caregiver to cue into a child's non-verbal and verbal communication is important. In addition, attempting and responding accurately to the situation and the child's emotional state is especially important when a child's communication development may be atypical due to a delay (Shire et al., 2016). Caregivers can use responsive interactions, which means caregivers are tuned into the child and join in the play, communications, and actions in the present moment to provide individualized support to promote social and emotional competence (Leifield & Sanders, 2007). Responsive interactions during play and daily activities are essential for development as children acquire and apply new information through them (Landry et al., 2012). This article discusses approaches for responsive interactions with infants, toddlers, and preschoolers.

After deciding whether to breastfeed or formula feed, parents must determine when to introduce solid foods. We examined feeding behaviors of 131 first-time parents, applying the theory of planned behavior to our investigation. Results indicated that parents' attitudes influenced infant feeding behavior at 6 months old. Therefore, Extension professionals should consider providing programming and materials to encourage healthful attitudes, in addition to focusing on general nutrition information. Moreover, our sample relied heavily on health professionals, friends and family, and books or videos for infant feeding information, so Extension professionals should consider targeting other influential groups in addition to targeting parents directly.

Introduction: Increased access to midwifery care is one strategy that could improve perinatal health outcomes and help address the maternal health crisis in the United States. A modifiable barrier to increasing the workforce is greater access to midwifery preceptors for clinical training. The objective of this research is to use the socioecological framework to identify midwives' perceptions of the barriers and facilitators to precepting students in clinical areas. Methods: Midwives attending a preceptor education and training workshop series responded to 3 different questions at the end of each session: (1) What makes precepting midwifery students challenging? (2) What makes precepting midwifery students possible? and (3) What makes precepting midwifery students worthwhile? Responses were coded to align with the socioecological framework, which distinguishes individual, interpersonal, community, institutional, and policy‐level influences. Results: Midwives' responses were spread across the levels of the socioecological model except for policy. Participants identified institutional influences such as support as factors that made precepting feasible, both individual and interpersonal factors such as time constraints as areas that presented challenges to precepting, and community factors, like the joy of sharing midwifery, contributing to what made precepting worthwhile. Discussion: Multiple levels of influence were identified in the preceptor process. Participants were internally motivated to precept while also articulating that to make precepting possible, there is a need for support from both colleagues and the greater systems within which they worked. Further studies are needed to investigate an ecosystem that facilitates an effective and sustainable model for midwifery precepting. Additionally, there is a need for efforts to engage and educate midwives in clinical practice about government advocacy that could actualize policy initiatives to support clinical midwifery education. [ABSTRACT FROM AUTHOR]

Throughout the history of television, American audiences have participated in a tradition of programs that follow a consistent structure: Amateur musicians and entertainers are offered an opportunity to display their talent on stage, competing for audience votes to win first prize and a chance at stardom. This article contributes to a growing literature on the significance of televised talent shows, demonstrating how their remarkable longevity and representational power stems from their configuration as a "format," the set of guidelines that structure and constrain the content of each broadcast—an aesthetic process grounded in exclusion. Through their formatting, I argue, these programs reify the notion of "talent" at the heart of talent shows, transforming a multidimensional and context-contingent assemblage of musical abilities into a seemingly stable object able to be recognized, rated, and ranked. Musical auditions offer a microcosm of formatting's role as a means of training audiences' attention. They normalize the practice of eliminating whatever (or whomever) is deemed unworthy—on these programs and in the wider world. Through analyzing examples from Ted Mack and the Original Amateur Hour (1948), The Gong Show (1978), and The Voice (2017), the article demonstrates how beneath the widely discussed content of contestant demographics, judge commentary, or audience voting results, the talent show format serves to obscure the contradictions upon which meritocracy's cruel optimism rests. [ABSTRACT FROM AUTHOR]

More research is needed to understand the relationships between imposter syndrome, perfection, and stress, as well as how they might be similar or different among first- and continuing-generation college students. This research study examines the relationships among imposter syndrome, perfectionism, and stress among first- and continuing-generation college students. Participants included 388 college students, including 184 (47.4%) first-generation students. Results indicate levels of imposter syndrome and stress are similar between first- and continuing-generation students. Levels of socially prescribed perfectionism are significantly correlated with imposter syndrome and stress for both groups; however, imposter syndrome is more strongly associated with stress among first-generation students. Implications for college student mental health and retention are discussed. [ABSTRACT FROM AUTHOR]

Research into intervention strategies for developmental verbal dyspraxia (DVD) clearly demonstrates the need to identify effective interventions. The goals of this study were to examine changes in articulation skills following the use of phonetic, multimodal intervention and to consider the relationship between these improved articulation skills and perceptions of resilience behaviors. These changes were related to components of the World Health Organization (WHO) International Classification of Functioning, Disability, and Health, Children and Youth version (ICF-CY). The intervention was implemented daily for 12 children diagnosed with DVD, ages 3 to 10 years, enrolled in a school for children with speech, language, and hearing impairments on a university campus in the southeast of the USA. Eleven children presented with significant comorbid conditions. Changes in articulation, along with changes in parents' and speech-language therapists' (SLTs') perceptions of resilience behaviors were measured. At the end of a 2-year period, statistically significant gains in articulation and parents' and SLTs' perceptions of resilience behaviors were noted. The relationship between improved articulation skills and increased resilience behaviors is discussed.

Colleges strive to increase leadership, service, and employability skills of their students; professional organizations are one way for students to learn such abilities. One AAFCS-accredited department has a student organization that has found relative success. Students indicated a desire for additional information about the field of family and consumer sciences and networking opportunities; they also noted that time conflicts created challenges to being active in the organization. Research is needed to investigate how the AAFCS core concepts can be promoted within student organizations.

Endobronchial valves (EBVs) are a novel, minimally invasive bronchoscopic management technique for persistent air leaks that fail conservative therapy. Currently, 2 EBVs are available in the United States: the Spiration Valve System (Olympus, Redmond, WA) and the Zephyr Valve (Pulmonx, Redwood, CA). These valves are Food and Drug Administration–approved to reduce hyperinflation in emphysematous patients via bronchoscopic lung-volume reduction. However, more recently, the Spiration Valve has been granted a compassionate use exemption through the Food and Drug Administration for persistent postsurgical air leaks. Despite their popularity, these devices are not free from side effects. As an anesthesiologist, it is vital to be aware of the pathophysiology of this patient population so that safe and effective anesthetics may be provided during valve placement. Here, the use of EBVs is discussed in a patient who presented with a persistent air leak after a transthoracic needle aspiration that failed treatment due to persistent hypoxemia, warranting EBV removal. [ABSTRACT FROM AUTHOR]

Previous research has explored family engagement experiences and practices in childcare programs. The purpose of this study was to explore family members' experiences related to their engagement in decision-making processes across various inclusive, toddler childcare programs. Five early educators and eight parents participated in the study. Four parents had a child with a diagnosed disability or developmental delays, were at-risk for developmental delays, and/or received speech, occupational, or physical therapy. Eligible early educators participating in the study taught toddlers (18 to 30 months) in a private, faith-based, or university childcare program. A semi-structured interview style was used to collect family participant responses. The early educators' inclusive practice indicator rubric scores previously collected from the larger grant funded project were used to triangulate data. Through a phenomenological qualitative design, this study gained a better understanding of families' decision-making experiences, facilitators and barriers that may impact family engagement, and opportunities early educators have provided or not provided to encourage family engagement in inclusive, toddler childcare programs. [ABSTRACT FROM AUTHOR]

Many women experience childhood sexual abuse (CSA) during their childhood and CSA often negatively impacts adult's romantic relationships. Consequently, it is important to understand the protective factors that buffer against the detrimental impact of CSA on the quality of women's romantic relationships. Forgiveness may be one such factor. The current study looked at trait forgiveness as a moderator of CSA and overall relationship quality, positive relationship quality, and negative relationship quality. A sample of 171 women completed an online survey. Using hierarchical regression, forgiveness was found to moderate the association between CSA and overall relationship quality and negative relationship quality, but not positive relationship quality. Findings indicate that the interaction between CSA and forgiveness was significant, but higher levels of forgiveness actually decreased overall relationship quality and increased negative relationship quality. The relationship between CSA and overall reports of relationship quality and negative relationship quality were stable at low levels of forgiveness, but when forgiveness was high overall relationship quality decreased and negative relationship quality increased. CSA was also directly associated with lower levels of positive relationship quality. Findings from the study indicate continued conceptual refinement when considering CSA, forgiveness, and relationship quality. [ABSTRACT FROM AUTHOR]

In the first study of the term "genius", 349 undergraduates nominated three geniuses in 1984, 1991, 1993, 1994, and 1997. Einstein was regarded as a stereotypical genius, while other nominees were subjective and transitory. In the second study, nominated geniuses were agreed to on only 26.2 percent of possible occasions. (Contains references.) (Author/CR)

Reactive oxygen species have been shown to play a significant role in hyperoxia-induced acute lung injury, in part, by inducing apoptosis of pulmonary endothelium. However, the signaling roles of phospholipid oxidation products in pulmonary endothelial apoptosis have not been studied. Using an oxidative lipidomics approach, we identified individual molecular species of phospholipids involved in the apoptosis-associated peroxidation process in a hyperoxic lung. C57BL/6 mice were killed 72 h after exposure to hyperoxia (100% oxygen). We found that hyperoxia-induced apoptosis (documented by activation of caspase-3 and -7 and histochemical terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling staining of pulmonary endothelium) was accompanied by nonrandom oxidation of pulmonary lipids. Two anionic phospholipids, mitochondria-specific cardiolipin (CL) and extramitochondrial phosphatidylserine (PS), were the two major oxidized phospholipids in hyperoxic lung. Using electrospray ionization mass spectrometry, we identified several oxygenation products in CL and PS. Quantitative assessments revealed a significant decrease of CL and PS molecular species containing [C.sub.18:2], [C.sub.20:4], [C.sub.22:5], and [C.sub.22:6] fatty acids. Similarly, exposure of mouse pulmonary endothelial cells (MLEC) to hyperoxia (95% oxygen; 72 h) resulted in activation of caspase-3 and -7 and significantly decreased the content of CL molecular species containing [C.sub.18:2] and [C.sub.20:4] as well as PS molecular species containing [C.sub.22:5] and [C.sub.22:6]. Oxygenated molecular species were found in the same two anionic phospholipids, CL and PS, in MLEC exposed to hyperoxia. Treatment of MLEC with a mitochondria-targeted radical scavenger, a conjugate of hemi-gramicidin S with nitroxide, XJB-5-131, resulted in significantly lower oxidation of both CL and PS and a decrease in hyperoxia-induced changes in caspase-3 and -7 activation. We speculate that cytochrome c driven oxidation of CL and PS is associated with the signaling role of these oxygenated species participating in the execution of apoptosis and clearance of pulmonary endothelial cells, thus contributing to hyperoxic lung injury. hyperoxia; endothelium; hydroperoxides; apoptosis doi: 10.1152/ajplung.00035.2010.

Children of maternal caregivers abused in childhood are at increased risk for mental health problems including anxiety and depression. To date, most studies exploring the intergenerational transmission of trauma have focused on younger children, with far fewer studies investigating adolescent mental health. Previous research suggests that maternal childhood abuse negatively impacts the parent–adolescent relationship, which may contribute to the development and maintenance of adolescent mental health problems. The current study examined dyadic reports of maternal–adolescent relationship quality as mediators linking maternal reports of childhood abuse to adolescent depression and anxiety. The bootstrapped indirect effects from maternal childhood abuse to adolescent symptoms of anxiety and depression were significant through adolescent reports of relationship quality, but not through maternal reports of relationship quality. Findings suggest that an adolescent's perception of their maternal–adolescent relationship may mediate the relationship between their maternal caregiver's childhood abuse and their own symptoms of anxiety and depression. [ABSTRACT FROM AUTHOR]

Helicobacter pylori (H. pylori) is a Gram-negative bacterium that colonizes the human stomach leading to the development of chronic gastritis, peptic ulcers and gastric adenocarcinoma. A combination of host, environment and bacterial virulence factors contribute to disease development. The H. pylori TNFα inducing protein (Tipɑ) is a virulence factor shown to induce multiple pro-inflammatory cytokines in addition to TNFα in vitro. The goal of the present study was to elucidate the role of Tipα in promoting inflammation in vivo and to identify the molecular pathways associated with Tipα associated virulence. Mice were infected with wild-type Sydney strain (SS1) or a tipα mutant (Δ tipα) for 1 month and 4 months. We also completed a second 4 months infection including a 1:1 SS1 to Δ tipα co-infected group in addition to SS1 and Δ tipα infected groups. The expression of TNFα, and KC were significantly higher in the SS1 infected group compared to both uninfected control (naïve) and Δ tipα groups. Mice infected with Tipα expressing SS1 induced more severe histological gastritis and developed hyperplasia compared to Δtipα infected mice. Microarray analysis of gastric epithelial cells co-cultured with recombinant Tipα (rTipα) demonstrates up-regulation of the NFκB pathway. This data suggest Tipα plays an important role in H. pylori induced inflammation. [ABSTRACT FROM AUTHOR]

Sexting is a common practice in intimate relationships; however, little research has investigated the role that sexting plays in adult relationships. The authors surveyed 356 individuals about their sexting behaviors and expectations and personality factors. Results indicated extroversion and openness are associated with greater arousal expectations for sending sexts and lower negative expectations for receiving sexts. Agreeableness is associated with lower negative expectations for sending sexts. Neuroticism is associated with lower arousal expectations and greater negative expectations for sending sexts. Individuals who are cohabitating sext more frequently than individuals who are dating, single, or married. [ABSTRACT FROM AUTHOR]

Parents of children with special needs can experience more stress in their marriage than parents of children without special needs. Divorce rates are higher for parents of children with special needs than for the general population, creating a need to study potential influencers of marital quality to promote healthy relationships within these families. Previous work has focused on the child as a major influence for the marital relationship, however this research sought to examine less commonly discussed factors that influence marital satisfaction for these parents. Perceived job flexibility and perceived spousal support in parenting were targeted as potential influencers, as they impact the amount of time and the quality of care an individual can provide. A mediation model was attempted to explain the mediating role of perceived spousal support in parenting in the relationship between perceived job flexibility and marital satisfaction. Results showed no correlation between perceived job flexibility and marital satisfaction. Positive correlations between perceived job flexibility and perceived spousal support in parenting were found and positive correlations were also seen between perceived spousal support in parenting and marital satisfaction. Analysis produced a more linear model of influence than the predicted mediation model. Implications of these findings were discussed. [ABSTRACT FROM AUTHOR]

I. INTRODUCTION No other human rights violation has been ignored and left unresolved as much as sexual violence against women. (1) The United Nations deferred formal recognition of rape as [...]

We sought to understand that individuals who sext in their marital relationships by looking at sexting behaviors in relation to both relationship status and marital satisfaction. Three hundred twenty-seven individuals completed the sexting behavior scales and the Kansas marital satisfaction scale. Results indicate that about 91% of the population report sending or receiving sext text messages. Although they still partake, this study also found that married individuals have a more negative view of sexting compared to other relationship statuses, except for younger females who despite their negative views of sexting, reported high marital satisfaction. Finally, sexting within the marital relationship does not seem to increase marital satisfaction. [ABSTRACT FROM AUTHOR]

Summary: Rhodococcus equi is a multihost, facultative intracellular bacterial pathogen that primarily causes pneumonia in foals less than six months in age and immunocompromised people. Previous studies determined that the major virulence determinant of R. equi is the surface bound virulence associated protein A (VapA). The presence of VapA inhibits the maturation of R. equi‐containing phagosomes and promotes intracellular bacterial survival, as determined by the inability of vapA deletion mutants to replicate in host macrophages. While the mechanism of action of VapA remains elusive, we show that soluble recombinant VapA32‐189 both rescues the intramacrophage replication defect of a wild type R. equi strain lacking the vapA gene and enhances the persistence of nonpathogenic Escherichia coli in macrophages. During macrophage infection, VapA was observed at both the bacterial surface and at the membrane of the host‐derived R. equi containing vacuole, thus providing an opportunity for VapA to interact with host constituents and promote alterations in phagolysosomal function. In support of the observed host membrane binding activity of VapA, we also found that rVapA32‐189 interacted specifically with liposomes containing phosphatidic acid in vitro. Collectively, these data demonstrate a lipid binding property of VapA, which may be required for its function during intracellular infection. [ABSTRACT FROM AUTHOR]

Bacteria are everywhere, out-numbering all other forms of life. The first organisms on earth were much like the bacteria today. Among the biological world, they have the widest range of [...]

Araucana chickens are known for their rounded, tailless rumps and tufted ears. Inheritance studies have shown that the rumpless (Rp) and ear-tufted (Et) loci each act in an autosomal dominant fashion, segregate independently, and are associated with an increased rate of embryonic mortality. To find genomic regions associated with Rp and Et, we generated genome-wide SNP profiles for a diverse population of 60 Araucana chickens using the 60 K chicken SNP BeadChip. Genomewide association studies using 40 rumpless and 11 tailed birds showed a strong association with rumpless on Gga 2 (Praw = 2.45x10-10, Pgenome = 0.00575), and analysis of genotypes revealed a 2.14 Mb haplotype shared by all rumpless birds. Within this haplotype, a 0.74 Mb critical interval containing two Iroquois homeobox genes, Irx1 and Irx2, was unique to rumpless Araucana chickens. Irx1 and Irx2 are central for developmental prepatterning, but neither gene is known to have a role in mechanisms leading to caudal development. A second genome-wide association analysis using 30 ear-tufted and 28 non-tufted birds revealed an association with tufted on Gga 15 (Praw = 6.61x10-7, Pgenome = 0.0981). We identified a 0.58 Mb haplotype common to tufted birds and harboring 7 genes. Because homozygosity for Et is nearly 100% lethal, we employed a heterozygosity mapping approach to prioritize candidate gene selection. A 60 kb region heterozygous in all Araucana chickens contains the complete coding sequence for TBX1 and partial sequence for GNB1L. TBX1 is an important transcriptional regulator of embryonic development and a key genetic determinant of human DiGeorge syndrome. Herein, we describe localization of Rp and Et and identification of positional candidate genes. [ABSTRACT FROM AUTHOR]

Objective The aim of the current study was to explore the health beliefs of older people in relation to skin cancer.Design This was a qualitative study, based on semi-structured interviews. Setting Interviews were carried out at a social centre, run by Age Concern, for people aged over 55.Methods Ten males and ten females, aged between 58 and 87, were interviewed using a semi-structured interview schedule.Results A qualitative content analysis of the interviews revealed a certain degree of confusion regarding the causes and appearance of skin cancer, and whilst early detection was perceived as 'a good thing' there was evidence of a lack of motivation, salience, knowledge of how to carry out skin self-examination, and a low sense of personal susceptibility.Conclusions It is suggested that health education for skin cancer needs to be targeted specifically at older people, in a way which encourages them to relate to it, in contrast to current health education which emphasises primary prevention and thus focuses on, and is designed to attract the attention of younger people. [ABSTRACT FROM PUBLISHER]

The article focuses on the importance of mental skills training to athletes. Mental skills training is said to focus on the positive aspects of athletes' performance, abilities and preparation while allowing them to gain more control over their performance. Results of a study found that time restrictions make it difficult for coaches to develop mental skills training. The study also mentioned that coaches need an integrated coaching team to help in the development of a comprehensive training program for athletes.

Self-focused attention is an important target of intervention within Wells's (2009) metacognitive therapy and the attention training technique (ATT) is one component of metacognitive therapy that purportedly alters focus of attention. However, we do not yet fully understand whether ATT causes changes in focus of attention, the effectiveness of ATT compared to other techniques in reducing self-focused attention, and how ATT leads to its therapeutic gains. A laboratory-based component study was completed to address these gaps in the literature. Nonclinical participants were randomly assigned to one session of ATT ( n = 38) or a mindfulness-based task ( n = 38). ATT and the mindfulness-based task differentially changed focus of attention, with ATT causing greater external focus of attention and the mindfulness-based task causing greater self-focused attention from pre-to-post manipulation. ATT and the mindfulness-based task both led to reductions in anxiety. Reductions in self-focused attention were related to less anxiety following ATT, whereas increases in self-focused attention were related to less anxiety following the mindfulness-based task. Conceptual and therapeutic implications are discussed. [ABSTRACT FROM AUTHOR]

The article shares efforts in antimicrobial use (AMU) data collection by the National Animal Health Monitoring System, administered by the U.S. Department of Agriculture (USDA) and discusses some of the challenges and opportunities in these arenas. The effort calls veterinarians and partners to action in supporting and participating in the collection of AMU data. Also mentioned are examples of treatment outcome data collection in food animal systems and challenges with AMU data collection.

AZD3783, a cationic amphiphilic drug and a potent inhibitor of the 5-hydroxytryptamine (5-HT1B) receptor, was explored as a potential treatment for depression. To support clinical trials, repeat dose toxicity studies in rats and dogs were conducted. Here we report toxicity findings in dogs after dosing from 1 to 3 months. In the 1-month study, there were minimal neuronal vacuolation in the brain, a marked increase in liver enzymes accompanied by hepatocellular degeneration/necrosis and phospholipidosis (PLD), and PLD/cholecystitis in the gallbladder of animals dosed at 47 mg/kg/day. In the 3-month study, neurotoxicity resulted in euthanasia of one animal dosed at 30 mg/kg/day after 86 days. Extensive pathologic changes were seen in all animals in retina epithelium (inclusion bodies), brain (neuronal vacuolation, degeneration, or necrosis and nerve fiber degeneration), spinal ganglia (vacuolation, degeneration, or necrosis), as well as sciatic and optic nerves (degeneration). Pigment-laden macrophages were observed in the lung, kidney, liver, gallbladder, bone marrow, gastrointestinal tract, and lymphoid tissues. Also seen were vitrel and retinal hemorrhage in the eyes. A brain concentration and pathology study showed that the concentration of AZD3783 in the brain was approximately 4 times higher than in the plasma after 4 weeks of dosing, however, they were similar in all regions examined, and did not correlate with areas with pathologic findings. Our findings with AZD3783 in dogs have not been reported previously with other CNS compounds that effect through serotonergic pharmacology. [ABSTRACT FROM AUTHOR]

Abstract: We have identified a new series of C-5 substituted indazolylaminoquinazolines as potent erbB2 kinase inhibitors. The lead compound 22 showed excellent in vitro potency, good physical properties, acceptable oral pharmacokinetics in rat and dog, and low human in vitro clearance. It showed at least equivalent activity dose for dose compared to lapatinib in various erbB2- or EGFR-driven xenograft models after chronic oral administration. [Copyright &y& Elsevier]

Abstract: Neutral 5-substituted 4-anilinoquinazolines addressed high in vivo clearance and phospholipidosis associated with previous basic compounds. A representative compound 8a inhibited tumor growth in a mouse xenograft model when co-administered with the cytochrome P450 inhibitor 1-aminobenzotriazole (ABT), and data are consistent with pharmacology primarily reflecting inhibition of erbB2 receptor tyrosine kinase. [Copyright &y& Elsevier]

The development and progression of cancer is controlled by gene expression, often regulated through chromatin packaging. Heterochromatin protein 1Hsα (HP1Hsα), one of three human HP1 family members, participates in heterochromatin formation and gene regulation. HP1Hsα possesses an amino-terminal chromodomain, which binds methylated lysine 9 of histone H3 (meK9 H3), and a carboxyl-terminal chromoshadow domain (CSD) that is required for dimerization and interaction with partner proteins. HP1Hsα is down-regulated in invasive metastatic breast cancer cells compared with poorly invasive nonmetastatic breast cancer cells. Expression of EGFP-HP1Hsα in highly invasive MDA-MB-231 cells causes a reduction in in vitro invasion, without affecting cell growth. Conversely, knock-down of HP1Hsα levels in the poorly invasive breast cancer cell line MCF-7 increased invasion, without affecting cell growth. To determine whether functions of the CSD were required for the regulation of invasion, mutant forms of HP1Hsα were expressed in MDA-MB-231 cells. A W174A mutation that disrupts interactions between HP1Hsα and PXVXL-containing partner proteins reduced invasion similar to that of the wild type protein. In contrast, an 1165E mutation that disrupts dimerization of HP1Hsα did not decrease invasion. No gross changes in localization and abundance of HP1Hsβ, HP1Hsγ, and meK9 H3 were observed upon expression of wild type and mutant forms of HP1Hsα in MDA-MB-231 cells. Taken together, these data demonstrate that modulation of HP1Hsα alters the invasive potential of breast cancer cells through mechanisms requiring HP1 dimerization, but not interactions with PXVXL-containing proteins. [ABSTRACT FROM AUTHOR]