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Objective: This study aims to investigate the therapeutic effect of Lactobacillus rhamnosus GG-loaded lemon pectin microspheres (LGG@LPM) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice. Methods: LGG@LPM was characterized, and the therapeutic potential of gavage administration of LGG@LPM against UC in mice was explored. Results: LGG@LPM microspheres exhibited good stability. The administration of LGG@LPM significantly alleviated the pathological process of DSS-induced UC in mice, restoring normal body mass and colon length and decreasing histological activity index (HAI) and disease activity index (DAI). Additionally, LGG@LPM administration reversed the increase in inflammatory cytokine levels and the decrease in antioxidant enzyme activities in the colon, the increase in serum lipopolysaccharide (LPS) levels and the alterations in short-chain fatty acids (SCFAs) levels induced by DSS in mice. 16S rRNA sequencing revealed that LGG@LPM restored the gut microbiota in UC mice through enhancing the abundance of Lactobacillus and Mucispirillum, and the alteration of the microbiota was significantly correlated with some environmental factors. Conclusion: LGG@LPM could alleviate the pathological progression of UC by improving the gut microbiota, the effect being more pronounced than that of either lemon pectin or LGG alone.

Featured Application: This work can be applied to estimate acceleration using measured inclination, withoutpartially measuring the target acceleration. Moreover, this is a finiteelement model-free method and can be conveniently applied to beam bridges. Data fusion is an important issue in bridge health monitoring. Through data fusion, specific unknown bridge responses can be estimated with measured responses. However, existing data fusion methods always require a precise finite element model of the bridge or partially measured target responses, which are hard to realize in actual engineering. In this study, we propose a novel data fusion method. Measured inclinations across multiple cross-sections of the target bridge and accelerations at a subset of these sections were used to estimate accelerations at the remaining sections. Theoretical analysis of a typical vehicle-bridge interaction (VBI) system has shown parallels with the blind source separation (BSS) problem. Based on this, Independent Component Analysis (ICA) was applied to derive surrogate inclination mode shapes. This was followed by calculating surrogate displacement mode shapes through numerical integration. Finally, a surrogate inter-section transfer matrix for both measured and unmeasured accelerations was constructed, enabling the estimation of the target accelerations. This paper presents three key principles involving the relationship between the surrogate and actual inter-section transfer matrices, the integration of mode shape functions, and the consistency of transfer matrices for low- and high-frequency responses, which form the basis of the proposed method. A series of numerical simulations and a large-scale laboratory experiment were proposed to validate the proposed method. Compared to existing approaches, our proposed method stands out as a purely data-driven technique, eliminating the need for finite element analysis assessment. By incorporating the ICA algorithm and surrogate mode shapes, this study addresses the challenges associated with obtaining accurate mode shape functions from low-frequency responses. Moreover, our method does not require partial measurements of the target responses, simplifying the data collection process. The validation results demonstrate the method's practicality and convenience for real-world engineering applications, showcasing its potential for broad adoption in the field. [ABSTRACT FROM AUTHOR]

Background: Cancer stem cells (CSCs) are a specific subpopulation of cancer cells with the ability of self‐renewal, infinite proliferation, multidifferentiation and tumorigenicity, and play critical roles in cancer progression and treatment resistance. CSCs are tightly regulated by the tumor microenvironment, such as hypoxia; however, how hypoxia regulates CSCs in non‐small cell lung cancer (NSCLC) remains unclear. Methods: The proportion of ALDHhi cells was examined using the Aldefluor assay. Tankyrase inhibitor XAV939 and siRNA were used to inhibit β‐catenin while pcDNA3‐β‐catenin (S33Y) plasmid enhanced the expression of β‐catenin. Western blot was administered for protein detection. The mRNA expression was measured by quantitative real‐time PCR. Results: We found that hypoxia led to an increase in the proportion of ALDHhi cells in lung squamous carcinoma (LUSC) H520 cells, while causing a decrease in the ALDHhi cell proportion in lung adenocarcinoma (LUAD) A549 cells. Similarly, β‐catenin expression was upregulated in H520 cells but downregulated in A549 cells upon exposure to hypoxia. Mechanically, the proportion of ALDHhi cells in both cell lines was decreased by β‐catenin inhibitor or siRNA knockdown, whereas increased after β‐catenin overexpression. Furthermore, hypoxia treatment suppressed E‐cadherin expression in H520 cells and enhanced N‐cadherin and β‐catenin expression, while this effect was completely opposite in A549 cells. Conclusion: The hypoxia‐EMT‐β‐catenin axis functions as an important regulator for the proportion of CSCs in NSCLC and could potentially be explored as therapeutic targets in the future. [ABSTRACT FROM AUTHOR]

Periodontitis is an inflammatory disease that eventually destroys tooth-supporting tissue. Yunnan Baiyao (YNBY), a traditional Chinese medicine compound with haemostatic and anti-inflammatory properties has shown therapeutic potential in several diseases. Our previous study revealed that YNBY suppressed osteoclast differentiation in periodontitis. The purpose of this study is to investigate the influences of YNBY on osteoblasts and explore its potential mechanisms. Materials and Methods: A rat periodontitis model was established by ligation of maxillary second molars. After the end of modelling, histopathological observation by hematoxylin-eosin (HE) staining and Masson trichrome staining, detection of bone resorption by Micro-CT scanning, detection of osteoclasts by tartrate-resistant acid phosphatase (TRAP) staining, expression of osteocalcin (OCN) and microtubule-associated protein 1 light chain 3 (LC3) by immunohistochemistry. Lipopolysaccharides was used to irritate MC3T3-E1 osteoblastic cells and ex vivo calvarial organ as an in vitro model of inflammation. CCK-8 assay was performed to examine the toxicity of YNBY to MC3T3-E1 osteoblastic cells. Osteogenesis was assessed with alizarin red staining, immunofluorescence staining, Western blot and immunohistochemical staining. Transmission electron microscopy, fluorescent double staining, Western blot and immunohistochemical staining were employed to detect autophagy. Results: Histological and micro-CT analyses revealed that YNBY gavage reduced bone loss caused by experimental periodontitis and upregulated osteogenic proteins in vivo. YNBY attenuated the production of autophagy-related proteins in periodontitis rats. Additionally, YNBY promoted osteogenesis by inhibiting inflammation-induced autophagy in vitro. Furthermore, YNBY suppressed LPS-mediated bone resorption and promoted the production of osteoblast-related proteins in inflamed calvarial tissues ex vivo. Conclusion: This study demonstrated, through in vivo, in vitro and ex vivo experiments, that YNBY promoted osteoblast differentiation by suppressing autophagy, which markedly alleviated bone destruction caused by periodontitis. [ABSTRACT FROM AUTHOR]

The quantitative evaluation of wetland hydrological connectivity is essential to the hydrological connectivity restoration‐oriented ecological conservation and environmental management of wetlands. We proposed a framework to evaluate wetland hydrological connectivity with a combination of hydrological connectivity metrics and morphological spatial pattern analysis and recognized potential sites and links that had been generally overlooked in previous studies. Variations in hydrological connectivity revealed a decreasing trend followed by a gradual recovery from the critical time node of 2005 in Baiyangdian Lake. The core, one of the most important landscape types, played a dominant role in maintaining wetland hydrological connectivity at both temporal and spatial scales, and its variations matched those of hydrological connectivity. More importantly, we redressed the conventional ignorance of peripheral patches and links and recognized their importance in improving the hydrological connectivity of wetlands. The proposed framework provides an effective and practical tool for the hydrological connectivity evaluation of wetlands, expanding new insights into maintaining the health and integrity of wetland ecosystems. Integr Environ Assess Manag 2023;19:1064–1078. © 2022 SETAC Key Points: We evaluated wetland hydrological connectivity from the multiscale and multifaceted perspectives with a combination of hydrological connectivity metrics and morphological spatial pattern analysis.We redressed the conventional ignorance of peripheral patches and links and recognized their importance in improving the hydrological connectivity of wetlands.The core, one of the most important landscape types, played a dominant role in maintaining wetland hydrological connectivity at both temporal and spatial scales, and its variations matched those of hydrological connectivity. [ABSTRACT FROM AUTHOR]

The unique physicochemical properties of DNA molecules allow them to be used as self‐assembling motifs for the construction of DNA nanostructures with the merits of shape designability, precise addressability, structural dynamic stimuli‐responsiveness, and superb biocompatibility. After loading therapeutical payloads, current DNA nanostructures were able to deliver them to diseased tissues and cells in a targeted manner, and release them responsively, as well as increase the cellular uptake of the payloads. In this review, we investigate the utility of typical DNA nanostructures in the development of the state‐of‐the‐art drug delivery systems, highlighting the key technological aspects in the transportation of payloads of different chemical categories, i. e. small molecules, oligonucleotides and peptides/proteins. Meanwhile, their implementation in various therapeutic modalities was surveyed, including chemotherapy, immunotherapy, gene therapy, protein therapy, photothermal and photodynamic therapy. And finally, possible challenges and future perspective are discussed for further developments. [ABSTRACT FROM AUTHOR]

Rare-earth-doped mixed crystals have demonstrated tunable optical properties, and it is of great importance to study the structural characteristics of the mixed-crystal hosts. Herein, LuxGd1-xVO4 (0 ≤ x ≤ 1) solid solution nanocrystals were synthesized by a modified sol–gel method, with a pure crystalline phase and element composition. The X-ray diffraction (XRD) and Rietveld refinement results showed that LuxGd1−xVO4 nanocrystals are continuous solid solutions with a tetragonal zircon phase (space group I41/amd) and the lattice parameters strictly follow Vegard's law. The detailed local structures were studied by extended X-ray absorption fine structure (EXAFS) spectra, which revealed that the average bond length of Gd-O fluctuates and decreases, while the average bond length of Lu-O gradually decreases with the increase in Lu content. Furthermore, the binding energy differences of core levels indicate that the covalent V-O bond is relatively stable, while the ionicity of the Lu-O bond decreases with the increasing x value, and the ionicity of the Gd-O bond fluctuates with small amplitude. The valence band structures were further confirmed by the first-principles calculations, indicating that the valence band is contributed to by the O 2p nonbonding state, localized Gd 4f and Lu 4f states, and the hybridized states between the bonding O 2p and V 3d. The binding energies of the Lu core and the valence levels tend to decrease gradually with the increase in Lu content. This work provides insight into the structural features of mixed-crystal hosts, which have been developed in recent years to improve laser performance by providing different positions for active ions to obtain inhomogeneous broadening spectra. [ABSTRACT FROM AUTHOR]

Determination of potassium ion concentration in biological samples is of great significance due to its vital role in the maintenance of physiological activities. Single or few‐layered transition metal dichalcogenides (TMD, e. g. MoS2, WS2, TiS2) have recently found wide applications in optoelectronics and biomedical engineering. Herein, for the first time, MoS2 nanosheets were integrated with G‐quadraplex DNA secondary structures to construct a fluorescence sensor for detecting potassium ions. The biosensor showed merits such as ease of preparation, ease of use, low‐cost, broad linear working range, as well as sufficient sensitivity, specificity and practical applicability, demonstrating its promise as an effective potassium ion sensor for practical applications. To the best of our knowledge, this is the first report on the combination of TMD nanomaterials and DNA quadraplex for the development of nanodevices. The proposed TMD nanosheets‐G‐quadraplex sensing formulation could enable the devise of other nanomachines and nanosensors considering the versatility of TMD nanomaterials and G‐quadraplex‐containing aptamers. [ABSTRACT FROM AUTHOR]

AGO2 is the only member of mammalian Ago protein family that possesses the catalytic activity and plays a central role in gene silencing. Recently researches reported that multiple gene silencing factors, including AGO2, function in the nuclei. The molecular mechanisms of the gene silencing factors functioning in nuclei are conducive to comprehend the roles of gene silencing in pretranslational regulation of gene expression. Here, we report that AGO2 interacts with DDX21 indirectly in an RNA-dependent manner by Co-IP and GST-Pulldown assays and the 2 proteins present nuclei foci in the immunofluorescence experiments. We found that DDX21 up-regulated the protein level of AGO2 and participated in target gene, SNM2 , alternative splicing involved in AGO2 by the indirect interaction with AGO2, which produced different transcripts of SMN2 in discrepant expression level. This study laid important experiment foundation for the further analysis of the nuclear functions of gene silencing components. [ABSTRACT FROM AUTHOR]

Summary: Fruit neck length (FNL) is an important quality trait in cucumber because it directly affects its market value. However, its genetic basis remains largely unknown. We identified a candidate gene for FNL in cucumber using a next‐generation sequencing‐based bulked segregant analysis in F2 populations, derived from a cross between Jin5‐508 (long necked) and YN (short necked). A quantitative trait locus (QTL) on chromosome 7, Fnl7.1, was identified through a genome‐wide comparison of single nucleotide polymorphisms between long and short FNL F2 pools, and it was confirmed by traditional QTL mapping in multiple environments. Fine genetic mapping, sequences alignment and gene expression analysis revealed that CsFnl7.1 was the most likely candidate Fnl7.1 locus, which encodes a late embryogenesis abundant protein. The increased expression of CsFnl7.1 in long‐necked Jin5‐508 may be attributed to mutations in the promoter region upstream of the gene body. The function of CsFnl7.1 in FNL control was confirmed by its overexpression in transgenic cucumbers. CsFnl7.1 regulates fruit neck development by modulating cell expansion. Probably, this is achieved through the direct protein–protein interactions between CsFnl7.1 and a dynamin‐related protein CsDRP6 and a germin‐like protein CsGLP1. Geographical distribution differences of the FNL phenotype were found among the different cucumber types. The East Asian and Eurasian cucumber accessions were highly enriched with the long‐necked and short‐necked phenotypes, respectively. A further phylogenetic analysis revealed that the Fnl7.1 locus might have originated from India. Thus, these data support that the CsFnl7.1 has an important role in increasing cucumber FNL. [ABSTRACT FROM AUTHOR]

Active cathode particles are fundamental architectural units for the composite electrode of Li‐ion batteries. The microstructure of the particles has a profound impact on their behavior and, consequently, on the cell‐level electrochemical performance. LiCoO2 (LCO, a dominant cathode material) is often in the form of well‐shaped particles, a few micrometres in size, with good crystallinity. In contrast to secondary particles (an agglomeration of many fine primary grains), which are the other common form of battery particles populated with structural and chemical defects, it is often anticipated that good particle crystallinity leads to superior mechanical robustness and suppressed charge heterogeneity. Yet, sub‐particle level charge inhomogeneity in LCO particles has been widely reported in the literature, posing a frontier challenge in this field. Herein, this topic is revisited and it is demonstrated that X‐ray absorption spectra on single‐crystalline particles with highly anisotropic lattice structures are sensitive to the polarization configuration of the incident X‐rays, causing some degree of ambiguity in analyzing the local spectroscopic fingerprint. To tackle this issue, a methodology is developed that extracts the white‐line peak energy in the X‐ray absorption near‐edge structure spectra as a key data attribute for representing the local state of charge in the LCO crystal. This method demonstrates significantly improved accuracy and reveals the mesoscale chemical complexity in LCO particles with better fidelity. In addition to the implications on the importance of particle engineering for LCO cathodes, the method developed herein also has significant impact on spectro‐microscopic studies of single‐crystalline materials at synchrotron facilities, which is broadly applicable to a wide range of scientific disciplines well beyond battery research. [ABSTRACT FROM AUTHOR]

Use of biologics has been widely advocated for inflammatory diseases recently. Anti-tumor necrosis factor (TNF)-α antibody therapy is reportedly effective against ocular inflammation. However, side effects of TNF-α inhibition have been reported, particularly in the form of exacerbation of infections such as tuberculosis. Paradoxical reactions such as exacerbated inflammation are also well known. Around 20 million humans are infected with human T-cell leukemia virus type 1 (HTLV-1) globally, and this virus can cause adult T-cell leukemia, HTLV-1-associated myelopathy and HTLV-1 uveitis. As for ophthalmic concerns, it has not been identified whether anti-TNF-α antibody stimulates HTLV-1-infected cells and ocular cells to induce HTLV-1 uveitis in HTLV-1 carriers. Here we investigated the effects of anti-TNF-α antibody on ocular status under HTLV-1 infectious conditions using ocular cells and HTLV-1-infected cells in vitro. We used the ARPE-19 human retinal pigment epithelial cell line as ocular cells considered to play an important role in the blood-ocular barrier, and the MT2 HTLV-1-infected cell line. Jurkat cells were used as controls. Infliximab (IFX) was used as an anti-TNF-α antibody to achieve TNF-α inhibition. We evaluated the production of inflammatory cytokines and intercellular adhesion molecule (ICAM)-1, proliferation of ARPE-19, expression of TNF-α receptor (TNF-R) and HTLV-1 proviral DNA, and the percentage of apoptotic ARPE-19. Inflammatory cytokines such as interleukin (IL)-6, IL-8, TNF, and ICAM-1 were significantly elevated through contact between ARPE-19 and MT2. Treatment with IFX tented to inhibit TNF production, although the level of production was low, but changes in IL-6, IL-8, and ICAM-1 remained unaffected. Expression of TNFR was unaltered by IFX treatment. HTLV-1 proviral DNA was not significantly changed with treatment. No change in cell growth rate or apoptotic rate of ARPE-19 was seen with the addition of IFX. In conclusion, IFX did not exacerbate production of inflammatory cytokines, and did not affect expression of TNFR, proliferation of ARPE-19, HTLV-1 proviral load, or apoptosis of ARPE-19. These results suggest that IFX does not exacerbate HTLV-1-related inflammation in the eye and represents an acceptable treatment option under HTLV-1 infectious conditions. [ABSTRACT FROM AUTHOR]

Combination with genomic DNA is one of the important ways for microRNAs (miRNAs) to perform biological processes. However, because of lack of an experimental method, the identified genomic sites targeted by microRNA were only located in the promoter and enhancer regions. In this study, based on affinity purification of labeled biotin at the 3′-end of miRNAs, we established an efficiently experimental method to screen miRNA binding sequences in the whole genomic regions in vivo. Biotinylated miR-373 was used to test our approach in MCF-7 cells, and then Sanger and next-generation sequencing were used to screen miR-373 binding sequences. Our results demonstrated that the genomic fragments precipitated by miR-373 were located not only in promoter but also in intron, exon, and intergenic. Eleven potentially miR-373 targeting genes were selected for further study, and all of these genes were significantly regulated by miR-373. Furthermore, the targeting sequences located in E-cadherin, cold-shock domain-containing protein C2 (CSDC2), and PDE4D genes could interact with miR-373 in MCF-7 cells rather than HeLa cells, which is consistent with our data that these three genes can be regulated by miR-373 in MCF-7 cells while not in HeLa cells. On the whole, this is an efficient method to identify miRNA targeting sequences in the whole genome. [ABSTRACT FROM AUTHOR]

The structural integrity of layered Ni-rich oxide cathode materials is one of the most essential factors that critically affect the performance and reliability of lithium-ion batteries. Prolonged battery operation often involves repeated phase transitions, buildup of mechanical stresses, and could provoke thermal spikes. Such sophisticated chemo-thermo-mechanical interplay can cause performance degradation through structural disintegration of the cathode active materials (CAMs). Herein, we systematically investigate the thermal decomposition, fracture, and oxygen evolution of chemically delithiated Li0.3Ni0.8Co0.15Al0.05O2 (NCA) particles upon heating from 25 °C to 450 °C using a number of advanced X-ray and electron probes. We observed a continuous reduction of the Ni oxidation state upon heating, as well as the release of oxygen from the NCA lattice that undergoes the thermally induced phase transformations. The release of oxygen also created numerous mesopores throughout the analyzed particles, which could significantly affect the chemical and mechanical properties of the electrode. In addition, intergranular and intragranular fracturing at elevated temperatures also contribute to the degradation of the NCA cathode under these conditions. Our investigation of the mechanical integrity at elevated temperatures provides a fundamental understanding of the thermally driven chemomechanical breakdown of the NCA cathode active materials. [ABSTRACT FROM AUTHOR]

While Li ion batteries are intended to be operated within a mild temperature window, their structural and chemical complexity could lead to unanticipated local electrochemical events that could cause extreme temperature spikes, which, in turn, could trigger more undesired and sophisticated reactions in the system. Visualizing and understanding the response of battery electrode materials to thermal abuse conditions could potentially offer a knowledge basis for the prevention and mitigation of the safety hazards. Here we show a comprehensive investigation of thermally driven chemomechanical interplay in a Li0.5Ni0.6Mn0.2Co0.2O2 (charged NMC622) cathode material. We report that, at the early stage of the thermal abuse, oxygen release and internal Li migration occur concurrently, and are accompanied by mechanical disintegration at the mesoscale. At the later stage, Li protrusions are observed on the secondary particle surface due to the limited lithium solubility in non-layered lattices. The extraction of both oxygen and lithium from the host material at elevated temperature could influence the chemistry and safety at the cell level via rearrangement of the electron and ion diffusion pathways, reduction of the coulombic efficiency, and/or causing an internal short circuit that could provoke a thermal runaway. [ABSTRACT FROM AUTHOR]

Novel developments in X‐ray sources, optics and detectors have significantly advanced the capability of X‐ray microscopy at the nanoscale. Depending on the imaging modality and the photon energy, state‐of‐the‐art X‐ray microscopes are routinely operated at a spatial resolution of tens of nanometres for hard X‐rays or ∼10 nm for soft X‐rays. The improvement in spatial resolution, however, has led to challenges in the tomographic reconstruction due to the fact that the imperfections of the mechanical system become clearly detectable in the projection images. Without proper registration of the projection images, a severe point spread function will be introduced into the tomographic reconstructions, causing the reduction of the three‐dimensional (3D) spatial resolution as well as the enhancement of image artifacts. Here the development of a method that iteratively performs registration of the experimentally measured projection images to those that are numerically calculated by reprojecting the 3D matrix in the corresponding viewing angles is shown. Multiple algorithms are implemented to conduct the registration, which corrects the translational and/or the rotational errors. A sequence that offers a superior performance is presented and discussed. Going beyond the visual assessment of the reconstruction results, the morphological quantification of a battery electrode particle that has gone through substantial cycling is investigated. The results show that the presented method has led to a better quality tomographic reconstruction, which, subsequently, promotes the fidelity in the quantification of the sample morphology. Automatic alignment of projection images with respect to a common rotation axis is fundamental to the quality of nano‐resolution tomographic reconstruction. A hybrid algorithm developed herein demonstrates superior accuracy, efficiency and robustness. [ABSTRACT FROM AUTHOR]

Grating‐based X‐ray differential phase‐contrast imaging has attracted a great amount of attention and has been considered as a potential imaging method in clinical medicine because of its compatibility with the traditional X‐ray tube source and the possibility of a large field of view. Moreover, phase‐contrast computed tomography provides three‐dimensional phase‐contrast visualization. Generally, two‐dimensional information retrieval performed on every projection is required prior to three‐dimensional reconstruction in phase‐contrast computed tomography. In this paper, a three‐dimensional information retrieval method to separate absorption and phase information directly from two reconstructed images is derived. Theoretical derivations together with numerical simulations have been performed to confirm the feasibility and veracity of the proposed method. The advantages and limitations compared with the reverse projection method are also discussed. Owing to the reduced data size and the absence of a logarithm operation, the computational time for information retrieval is shortened by the proposed method. In addition, the hybrid three‐dimensional images of absorption and phase information were reconstructed using an absorption reconstruction algorithm, hence the existing data pre‐processing methods and iterative reconstruction algorithms in absorption reconstruction may be utilized in phase reconstruction immediately. [ABSTRACT FROM AUTHOR]

Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the metacestode larva of Echinococcus granulosus. In this study, two-dimensional gel electrophoresis (2-DE) coupled with immunoblot analysis revealed that E. granulosus severin and 14-3-3zeta proteins (named EgSeverin and Eg14-3-3zeta, respectively) might be two potential biomarkers for serological diagnosis of echinococcosis. The recombinant EgSeverin (rEgSeverin, 45 kDa) and Eg14-3-3zeta (rEg14-3-3zeta, 35 kDa) were administered subcutaneously to BALB/c mice to obtain polyclonal antibodies for immunofluorescence analyses (IFAs). And IFAs showed that both proteins were located on the surface of protoscoleces (PSCs). Western blotting showed that both proteins could react with sera from E. granulosus- infected sheep, dog, and mice. Indirect ELISAs (rEgSeverin- and rEg14-3-3zeta-iELISA) were developed, respectively, with sensitivities and specificities ranging from 83.33% to 100% and a coefficient of variation (CV %) of less than 10%. The rEgSeverin-iELISA showed cross-reaction with both E. granulosus and E. multilocularis , while the rEg14-3-3zeta-iELISA showed no cross-reaction with other sera except for the E. granulosus- infected ones. The field sheep sera from Xinjiang and Qinghai were analyzed using rEgSeverin-iELISA, rEg14-3-3zeta-iELISA, and a commercial kit respectively, and no significant differences were found among the three methods (p > 0.05). However, the CE positive rates in sheep sera from Qinghai were significantly higher than those from Xinjiang (p < 0.01). Overall, the results suggest that EgSeverin and Eg14-3-3zeta could be promising diagnostic antigens for E. granulosus infection. • Both EgSeverin and Eg14-3-3zeta were located on the surface of protoscoleces of E. granulosus. • The rEgSeverin-iELISA could be used in the detection of anti- E. granulosus or anti- E. multilocularis sera. • The rEg14-3-3zeta-iELISA could only be employed for the detection of anti- E. granulosus sera. • It is the first time to develop the iELISAs based on two antigens-EgSeverin and Eg14-3-3zeta. [ABSTRACT FROM AUTHOR]

Formaldehyde (FA) is a human leukemogen. Since there is a latency period between initial FA exposure and the development of leukemia, the subsequent impact of FA on hematopoietic stem or progenitor cells (HSCs/HPCs) in post-exposure stage is crucial for a deep understanding of FA-induced hematotoxicity. BALB/c mice were exposed to 3 mg/m 3 FA for 2 weeks, mimicking occupational exposure, and were monitored for another 7 days post-exposure. Meanwhile, we included benzene (BZ) as a positive control, separately and together with FA because co-exposure occurs frequently. After 7-day recovery, colonies of progenitors for CFU-GM and BFU-E, and nucleated bone marrow cells in FA-exposed mice were comparable to controls, although they were significantly reduced during exposure. Levels of reactive oxygen species (ROS) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in CFU-GM and BFU-E from FA-exposed mice were higher than controls, although the increase in 8-OHdG was not significant. Granulocyte-macrophage colony stimulating factor (GM-CSF) level in the FA group was lower than controls, but the expression level for the receptor was not upregulated. It suggests that HSCs/HPCs in FA-exposed mice respond to a small amount of GM-CSF and proliferate rapidly, which may cause a possible risk of expansion of abnormal stem/progenitor cell clones. FA co-exposure with BZ was more potent for promoting CFU-GM formation and inducing ROS in BFU-E and 8-OHdG in CFU-GM during the post-exposure period. The compensation of myeloid progenitors with elevated ROS and 8-OHdG may lead to a risk of transforming normal HSCs/HPCs to leukemic stem/progenitor cells. Thus, co-exposure may pose a greater leukemia risk. [ABSTRACT FROM AUTHOR]