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25. Polo-like kinase 1 promotes pulmonary hypertension

Author

Chen, Rongrong, Wang, Hongfei, Zheng, Cuiting, Zhang, Xiyu, Li, Li, Wang, Shengwei, Chen, Hongyu, Duan, Jing, Zhou, Xian, Peng, Haiyong, Guo, Jing, Zhang, Anchen, Li, Feifei, Wang, Wang, Zhang, Yu, Wang, Jun, Wang, Chen, Meng, Yan, Du, Xinling, and Zhang, Hongbing

Published
2023
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27. Study on permeability model of bedding coal and rocks based on equivalent fracture aperture

Author

QI Xianyin, WANG Shengwei, GENG Diandong, FU Peng

Subjects
gas extraction, bedded coal and rock, equivalent fracture aperture, permeability, gas-solid coupling, Mining engineering. Metallurgy, TN1-997
Abstract

In order to improve the anisotropy characteristics of permeability model in quantitatively describing mechanical deformation and permeability behavior of bedding coal rocks, take the fracture aperture of parallel bedding coal rocks as the benchmark, introduce the equivalent fracture aperture coefficient, and make the fracture aperture of non-parallel bedding coal rocks equivalent, we propose a permeability evolution model of bedding coal and rocks based on equivalent fracture aperture coefficient. The results show that with the increase of the bedding angle, the permeability of bedding coal and rocks decreases significantly, and the larger the bedding angle is, the smaller the dynamic permeability changes with the change of gas pressure; the adsorption effect has a significant impact on the evolution of permeability, but they are in a competitive relationship under different mechanical boundaries. The effect of effective stress on permeability is in a dominant position under the boundary of constant surrounding rock stress, while the adsorption effect is dominant under the fixed displacement boundary condition; for gas drainage of bedding coal and rock, drainage along the bedding direction can greatly improve the drainage efficiency.

Published
2023
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41. Structural Characterization of Longan Polysaccharide and Its Activating Effect on Macrophages

Author

ZHANG Jing, FENG Chao, WANG Shengwei, YANG Ruili, LI Wu

Subjects
longan, polysaccharide, purification, structural characterization, macrophages, Food processing and manufacture, TP368-456
Abstract

To explore the structure of longan polysaccharide and its effect on macrophages, a homogenous polysaccharide (named LP) was isolated and purified from longan pulp by ethanol fractional precipitation, and the structure of LP was characterized by gel permeation chromatography with laser light scattering (GPC-LLS), infrared spectroscopy (IR) and nuclear magnetic resonance (NMR) spectroscopy. Meanwhile, its effect on the phagocytic capacity of macrophages and the secretion of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. The results showed that LP was a heteropolysaccharide with a weight average molecular mass of 8.42 × 105 Da composed of glucose, galactose, mannose, mannuronic acid, arabinose and rhamnose at a molar ratio of 98.70% : 0.57% : 0.35% : 0.09% : 0.20% : 0.12%. NMR spectroscopic analysis showed the backbone chain structure of LP to be 6)-α-Glcp-(1→6)-α-Glcp-(14)-α-Glcp-(-1. LP at 50, 100, 200 μg/mL promoted the phagocytic capacity of macrophages and increased the secretion of TNF-α, IL-1β and IL-6. The phagocytic capacity of LP-induced macrophages increased by 95.61% compared with the untreated control group. TNF-α, IL-1β and IL-6 secretion in macrophages challenged by LP at 50, 100 and 200 μg/mL were 2.38, 10.51 and 10.67 times higher than those in the control group, respectively. These results showed that LP had an activating effect on macrophages, which can provide a scientific basis for the deep processing of longan fruit.

Published
2023
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