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The experiment was to investigate the effect of different proportions of Moringa oleifera leaves polysaccharides on the growth performance, serum biochemical indexes and immune function of Sanhuang chickens. A total of 600 oneday- old Sanhuang chickens were randomly divided into five groups with eight replicates in each group and 15 chickens in each replicate. The chickens in the control group (CON group) were fed a basal diet, and the basal diet of group T1, group T2, group T3 and group T4 was supplemented with 0.08%, 0.12%, 0.16% and 0.20% Moringa oleifera leaves polysaccharides, respectively. The trial period was 42 days. The results showed that compared with the control group, the feed to gain ratio of Sanhuang chickens in the group T1, group T2 and group T3 from 1 to 21 days of age decreased significantly (P<0.05), and the body weight of Sanhuang chickens in group T3 at 42 days of age increased significantly (P<0.05). From 1 to 42 days of age, the feed to gain ratio of Sanhuang chickens in the experimental groups decreased significantly (P<0.05). Compared with the control group, the content of serum albumin in the group T2 increased significantly (P<0.05), while the content of serum urea nitrogen in the group T3 decreased significantly (P<0.05). The glucose content in serum of the group T1, group T2 and group T3 increased significantly (P<0.05). The thymus index of the group T3 increased significantly ( P<0.05), the serum IL-4 content of the group T1, group T3 and group T4 increased significantly (P<0.05), the serum IL-6 content of the group T2 and group T3 decreased significantly (P<0.05), and the serum IL-1β content of the experimental groups increased significantly ( P<0.05). The experiment indicates that adding Moringa oleifera leaves polysaccharides can improve the growth performance, promote the development of immune organs, improve immune function and improve protein metabolism of Sanhuang chickens, and adding 0.16% has the better effect. [ABSTRACT FROM AUTHOR]