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Coral reefs, widely distributed in tropical and subtropical waters, generally have uneven flat topography. Sudden changes in reef flat topography significantly impact the propagation and evolution characteristics of tsunami-like waves; however, related studies are rare. This study systematically investigates the propagation and evolution of tsunami-like waves over uneven islands and reefs through wave flume physical experiments, analyzing the influences of incident wave height and reef flat water depth on these characteristics. The experimental results show that an uneven reef flat blocks the propagation of solitary waves and generates reflected waves. The slope, reef margin, and first reef flat in front of the reef are deep underwater, causing solitary waves to undergo shallow deformation without breaking. The wave height increases to a maximum at the step of the reef flat at shallower water depths and then breaks on the second reef flat. After breaking, the solitary wave propagates over the second reef flat as a swell, with wave height slowly attenuated due to energy dissipation from breaking in the limited water depth. As the incident wave height increases, the local wave height at each measuring point rises, with the increase in wave surface further amplified by the shallowing effect at the reef flat step. An increase in incident wave height causes the uneven reef flat to dissipate more wave energy and enhances wave attenuation. Consequently, the spatial distribution of local wave height Hi/H0 decreases with an increase in incident wave height. Increasing the water depth of the reef flat gradually weakens the shallowing effect, resulting in a decrease in the range and peak value of the maximum wave height near the reef flat step. When the water depth of the reef flat exceeds a certain limit, such as hr = 0.05 m in this study, the influence of the uneven reef flat on local wave height diminishes. An increase in the water depth leads to a reduction in wave energy consumed by the uneven reef flat, while the maximum wave height on the second reef flat increases monotonically with deeper water. During the propagation of solitary waves over uneven islands and reefs, two reflections from reef margins and flat steps occur. The reflection coefficient CR1 decreased with increasing incident wave height and reef flat water depth, while the reflection coefficient CR2 decreases significantly with increasing water depth. When the reef flat water depth is low, sudden changes in reef flat terrain greatly enhance the reflection intensity of incident waves. In particular, when hr = 0 m, the reflection coefficient CR2 reaches approximately 0.3.

As a new type of grain storage structure, the double-story squat silo (DSSS) can ensure the quality of grain storage, but also has the advantages of land saving, energy saving and carbon reduction, and high degree of mechanization. In this paper, the method of coupling discrete elements and finite elements was adopted to construct a mathematical analysis model of the dynamic response between grain particles and the silo wall during the central unloading process in a double-layer shallow round silo (DSSS). The validity of the scaling method was verified by combining experimental tests with the scaling model. Numerical analyses of the scaled-down model were compared with theoretical calculations to confirm the feasibility of the numerical methods, and the grain-silo interaction during the central unloading of the DSSS was investigated. The results showed that both the upper and lower layers exhibited a sudden increase in lateral pressure and oscillation during the unloading process, with a larger overpressure coefficient and a greater tendency for overpressure to occur in the middle of the silo wall. The stresses in both layers of the silo wall reached their maximum at 1/4 of the distance from the bottom of the silo wall when considering the grain-silo interaction. This study has provided technical support for the structural design of DSSS, facilitating its promotion and application, and had significant engineering implications.

Sport plays a crucial role in human society, fostering physical health, collaboration, and the spirit of rivalry. The importance of sports in promoting a sense of well-being, self-control, and cognitive sharpness should be considered. The efficacy of sports and athletic performance often relies on the caliber of clothing used. Traditional sportswear encounters many issues, including but not limited to restricted breathability, inefficient moisture management, and insufficient ultraviolet (UV) protection. To examine these concerns, the present study investigates the Intelligent Sportswear Design (ISWD), an innovative advancement rooted in conjugated nanomaterials. The field of ISWD covers the development of sports apparel and footwear specifically intended to boost performance and provide optimal comfort. Coatings of graphene, silver nanoparticles, and environmentally friendly green nanomaterials are employed in these multipurpose PES fabrics. This research looks at how we might better protect athletes from the sun by developing fabrics with UV-blocking qualities. Results show increased moisture-wicking efficiency, better UV-radiation protection, and enhanced electrical conductivity. The study found that fabric moisture management could be improved by 25%, UV-blocking performance could be increased by 30%, fabric conductivity could be increased by 15%, and heat retention could be decreased by 20%. The experimental results show that the proposed ISWD exhibits notable performance in several necessary measures, including moisture-wicking effectiveness (23.22%), UV-blocking performance (34.22%), fabric conductivity (20.88%), heat retention reduction (26.94%), and UV radiation shielding (35.68%).

Abstract Background Circular RNAs (circRNAs) are capable of affecting breast cancer (BC) development. However, the role and underneath mechanism of circFKBP8 (also known as hsa_circ_0000915) in BC remain largely unknown. Methods Expression analyses were performed using quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemistry (IHC) assays. Effects on cell functional phenotypes were determined by assessing cell proliferation, migratory capacity, invasion, and stemness in vitro. The relationship between microRNA (miR)-432-5p and circFKBP8 or E2F transcription factor 7 (E2F7) was examined by RNA pull-down, dual-luciferase reporter, and RNA immunoprecipitation (RIP) assays. Xenograft assays were used to identify the function of circFKBP8 in vivo. Results CircFKBP8 was presented at high levels in BC tissues and cells. High circFKBP8 expression was associated with worse overall survival in BC patients. CircFKBP8 suppression inhibited BC cell proliferation, migratory capacity, invasion and stemness in vitro. CircFKBP8 suppression blocked xenograft tumor growth in vivo. Mechanistically, circFKBP8 functioned as a miR-432-5p sponge to modulate E2F7 expression. CircFKBP8 modulated BC cell malignant behaviors by miR-432-5p, and miR-432-5p affected these cell phenotypes through E2F7. Conclusion Our observations prove that circFKBP8 promotes BC malignant phenotypes through the miR-432-5p/E2F7 cascade, offering a promising therapeutic and prognostic target for BC.

Abstract Background Does incorporating Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors into endocrine therapy (ET) effectively enhance survival outcomes, notably overall survival (OS), among individuals with hormone receptor-positive (HR+), human epidermal growth factor receptor 2-negative (HER2-) advanced breast cancer? This remains a clinical controversy. We compared the antitumor efficacy and adverse effects (AEs) between CDK4/6 inhibitors + ET (CET) and placebo + ET (PET) by conducting a phase III randomized controlled trials (RCTs) based meta-analysis. Methods Seven databases were searched to identify eligible studies, comprising Phase III RCTs comparing CET to PET. The primary endpoints were OS and progression-free survival (PFS), with secondary endpoints including responses and adverse events (AEs). Results Seven RCTs (DAWNA-2, MONALEESA-2, MONALEESA-3, MONALEESA-7, MONARCH-3, PALOMA-2, and PALOMA-4) were included. The CET group exhibited significantly improved OS (HR: 0.81 [0.74, 0.88]), PFS (HR: 0.57 [0.52, 0.63]), objective response rate (RR: 1.31 [1.20, 1.43]), and clinical benefit rate (RR: 1.11 [1.07, 1.15]). These benefits were consistent across almost all subgroups. Additionally, the CET group showed better overall survival rates (OSR) from 24 to 60 months (OSR 24–60 m) and progression-free survival rates (PFSR) from 6 to 60 months (PFSR 6–60 m). However, more total AEs, grade 3–5 AEs, and serious AEs were found in CET group. The top 5 grade 3–5 AEs in the CET group were neutropenia (59.39%), leukopenia (24.11%), decreased white blood cell count (12.99%), hypertension (7.03%), and increased alanine aminotransferase (5.91%). Conclusions The superiority of CET over PET in HR+/HER2- advanced breast cancer is evident, showing improved survival and responses. Nonetheless, the higher incidence of AEs, specifically hematologic AEs, requires cautious attention.

Breast cancer is the most diagnosed cancer in women worldwide and the leading cause of most cancer-related deaths, posing a serious threat to women′s health worldwide. At present, although the prognosis of some patients with breast cancer has improved, the emergence of drug resistance and the metastasis and recurrence of breast cancer are still the main reasons for poor prognosis. CD36 is a multiligand transmembrane glycoprotein expressed on various cell types. In recent years, studies have confirmed that CD36 can reshape the lipid metabolism of cancer cells; promote the differentiation of tumor-related macrophages into M2 type and recruitment into tumor tissues; regulate the function of Treg cells, CD8+ T cells, DCs, and other immune cells, and thus promote tumor development. In addition, CD36 is also associated with breast cancer stem cells, metastasis-initiating cells, and breast drug resistant cells. Therefore, CD36 could be an important potential therapeutic target for breast cancer.

In the dynamic interaction between the runner and the ground, the running shoe is the only medium that bears the impact force of body weight and plays a crucial role in athletic performance. Traditional designs do not adequately consider the different shapes of the foot, which often leads to discomfort and aggravation of foot disorders. This study presents an innovative approach to running shoe midsole design using 3D-printed chiral negative poisson’s ratio (NPR) structures to enhance shock absorption and support, thereby optimizing biomechanical performance and comfort. Using computer-aided design (CAD) and computer-aided engineering (CAE), the biomechanical effects of different midsole structures has been explored through finite element analysis (FEA). The study focuses on optimizing the cushioning, propulsion and stability of the midsole to mitigate the impact on the ankle and knee. Static compression and dynamic impact simulations were utilized to comprehensively select optimized design of midsole structure and the selected structures was 3D printed to validate the biomechanical benefits in a wear trial. The results of the study highlight the superior performance of chiral NPR structures in reducing forefoot stress during standing and movement and advance the design and functionality of 3D printed materials in running shoes.

Objective To observe the effect of abdominal massage combined with moxibustion in the treatment of Qi-deficiency type chronic functional constipation in elderly people. Methods Totally 100 elderly patients with chronic functional constipation of Qi-deficiency type were randomly divvied into the control group and observation group, with 50 cases in each group. The control group received routine treatment and abdominal massage, and the observation group received abdominal massage combined with moxibustion. The defecation condtion, Tradition Chinese Medicine syndrome score and clinical curative effect were compared between two groups. Results The Patient Assessment of Constipation Symptoms(PAC-SYM) score was decreased in both two groups after intervention (P＜0. 05), and PAC-SYM score in the observation group was lower than that in the control group (P＜0. 05). The Tradition Chinese Medicine syndrome score was decreased in both two groups after intervention (P＜0. 05), and Tradition Chinese Medicine syndrome score in the observation group was lower than that in the control group (P＜0. 05). The total effective rate was 94. 00%(47/50) in the observaiton group, which was higher than 76. 00%(38/50) in the control group (P＜0. 05). Conclusion The abdominal massage combined with moxibustion is effective to relieve the clinical symptoms of Qi-deficiency type chronic functional constipation in elderly people. (目的 观察腹部推拿联合艾灸治疗气虚型老年慢性功能性便秘的效果。方法 选取2022年4月—2023年12月在医院住院治疗的气虚型老年慢性功能性便秘患者100例为研究对象, 采用随机数字表法分为对照组和观察组, 各50例。对照组给予常规治疗和腹部推拿, 观察组在对照组基础上联合艾灸治疗。治疗7 d后比较两组患者排便情况、中医证候积分及临床疗效。结果 治疗后两组便秘患者症状自评量表(PAC-SYM)评分均较治疗前降低(P＜0. 05), 且观察组治疗后评分低于对照组(P＜0. 05)。治疗后两组排便无力、腹中隐痛、食欲不振、乏力懒言等中医证候积分均较治疗前降低(P＜0. 05), 且观察组治疗后评分低于对照组(P＜0. 05)。观察组总有效率为94. 00%(47/50), 高于对照组的76. 00%(38/50), 差异有统计学意义(P＜0. 05)。结论 腹部推拿联合艾灸治疗可有效缓解气虚型老年慢性功能性便秘患者的临床症状。)

Small nucleolar RNAs (snoRNAs) are non-coding RNAs in the nucleolus and are mainly responsible for the 2'-O-methylation and pseudouridine modification of rRNA. snoRNAs regulate various biological processes, such as tRNA modification, spliceosome snRNA modification, maintenance of the telomere structure, and alternative splicing of mRNA. Aberrant expression of snoRNA is related to cancer progression, and it may become a new target for cancer treatment. snoRNAs are stable and easy to detect in body fluids, so they can be used as a biomarker for clinical diagnosis and prognostic. This article reviews the biogenesis, classification, structure, and function of snoRNAs and introduces their potential role in the occurrence and development of hepatocellular carcinoma.

Hematopoietic stem cell transplantation (HSCT) has been widely used in the treatment of malignant hematological diseases, non-malignant refractory hematological diseases and some solid tumors. Preconditioning of HSCT will inevitably cause damage to the gastrointestinal tract and increase the incidence of malnutrition. Malnutrition can affect hematopoietic reconstitution, increase the incidence of acute graft-versus-host disease (aGVHD) and infection, and prolong hospital stay. At present, there are few reports on systematic nutritional intervention in patients with hematopoietic stem cell transplantation. This article reviewed the nutritional risk screening and assessment tools, nutritional support methods and nutritional intervention models for patients undergoing HSCT, and provided reference for establishing related nutritional plan. (造血干细胞移植(HSCT)被广泛应用于恶性血液病、非恶性难治性血液病和某些实体瘤的治疗。HSCT预处理会对胃肠道造成不可避免的损伤, 增加营养不良风险。营养不良会影响造血功能重建, 增加急性移植物抗宿主病( aGVHD)及感染的发生率, 延长住院时间, 严重影响HSCT患者生存质量。本研究就HSCT患者营养风险筛查和评估工具、营养支持方式及营养干预模式进行综述, 旨在为HSCT患者营养干预方案的制定提供参考。)

In this paper, we consider a class of functionals subject to a duality restriction. The functional is of the form J(Ω,Ω*)=∫Ωf+∫Ω*g $\mathcal{J}\left({\Omega},{{\Omega}}^{{\ast}}\right)={\int }_{{\Omega}}f+{\int }_{{{\Omega}}^{{\ast}}}g$ , where f, g are given nonnegative functions in a manifold. The duality is a relation α(x, y) ≤ 0 ∀ x ∈ Ω, y ∈ Ω*, for a suitable function α. This model covers several geometric and physical applications. In this paper we review two topological methods introduced in the study of the functional, and discuss possible extensions of the methods to related problems.

Starch serves as the primary energy source for human activities and is utilized as a crucial raw material in various industries. However, due to limited land resources and challenging yield growth, the traditional starch-producing plants are significantly influenced by environmental conditions, which made it unable to meet the demands of population expansion. On the other hand, the traditional source of starch exhibits a relatively stable structure but faces performance limitations that fail to fully meet application requirements. Consequently, structural modifications have been often employed to effectively regulate the performance of starch. Chemical modification is a commonly employed method for controlling performance, but it can pose potential risks to food safety due to the occurrence of chemical reactions caused by limited reagents, residues of chemical auxiliary materials, generation of by-products, and the introduction of new chemical groups into starch molecules. Therefore, the traditional starch resources are inadequate to meet the demands of sustainable development. It is imperative to explore novel starch resources possessing “future characteristics”. According to their characteristics, the sources of future starch could be divided into three categories: firstly, the traditional starch source plants could be genetic breeding or gene edited to achieve directional or site-directed transformation of starch structure, making starch with special application performance; secondly, new starch resources that could overcome the limitations of land and natural environment, or are rich in starch but have not been fully developed and utilized; thirdly, new cell-free synthetic starch that does not take plants and microorganisms as carriers. This review elucidates the necessity, feasibility and development trend of future starch development, and emphasizes on the research status and characteristics of three types of future starch, taking high amylose starch, waxy starch, duckweed starch, microalgae starch, CO2 synthetic starch and polysaccharide biosynthesis starch as examples. This review will provide valuable guidance for future advancements in starch development.

The process from test starting to supersonic flow state established in an outlet-opening wind tunnel，i. e. the starting problem of supersonic flow，has become a recognized challenge. In order to establish the feasible flow field starting method，and lay the foundation for the use of outlet-opening wind tunnel，three-dimensional numerical simulation research is carried out，which is based on a certain wind tunnel，and taken the panel blade of supersonic compressor as the research object. The failure reason of supersonic flow field starting on experimental condition is analyzed，and three flow field starting schemes are established. The results show that the starting failure reason is the formation of a strong normal shock wave at the leading edge of the cascade. Increasing the inlet total pressure alone cannot establish the supersonic flow. The supersonic cascade flow field can be started by increasing the overflow gap width on lower wall side，but the number of effective cascade passages is decreased and the boundary layer thickness is increased. Maintaining the width of overflow gap on the upper and lower walls at more than 1.0 pitch，the setting upper and lower supersonic walls in front of cascade and conducting suction can effectively start the supersonic flow field. The maximum fluctuation of outlet Mach number is 0.01，the maximum fluctuation of outlet flow angle is 0.09°. The periodicity can meet the experimental requirements.

In order to explore the failure behavior of heat-insulating and abradable seal coating，the effects of interface roughness and the abradable structure of the surface layer near the interface on the cracking of the composite coating were investigated.The cohesive zone model and the phase-field damage method were used to describe the overall peeling of cracks along the interface and the internal fracture behavior of abradable surface layer.Moreover，the peeling failure mechanism of coating was revealed.Results showed that the bonding strength and cracking tendency of the coating presented opposite change laws with the increase of interface roughness.On the one hand，the effective contact area of the non-damaging composite coating interface increased，and the bonding strength increased.On the other hand，with the increase of roughness，the internal fracture of the surface layer became easier.Therefore，the interface roughness should be optimized reasonably.In addition，the pore of surface layer near the interface could reduce the bonding strength at the interface.Based on this，an appropriate interface roughness was selected，and an anti-cracking compact transition layer was introduced between the thermal insulation layer and the abradable surface layer to eliminate the weakening effect of surface layer pores on the interface strength of composite coatings and design an anti-cracking coating.

ObjectiveTuberous sclerosis complex (TSC) is a rare autosomal dominant genetic disease involving multiple organ system. Renal angiomyolipoma (RAML) is a leading cause of death for adult TSC patients. Our study aims at investigating the clinical manifestations of TSC-RAML to enable clinicians to have a better understanding of the disease in Chinese patients.MethodsWe reviewed retrospectively the data collected from patients with TSC-RAML in Peking Union Medical College Hospital from January 2014 to January 2023. We also collected information about the age, gender, imageological examination, RAML stage, and comorbidities.ResultsA total of 186 TSC-RAML patients were registered, 65 of whom were males and 121 were females(male-to-female ratio 1∶1.86). The median age of all patients was 31 years old. Totally, 117 cases (62.9%)of RAML were rated stage 6. Twenty-two cases (11.8%) had a history of tumor rupture and bleeding. Research shows statistical differences between high and low grade on RAML rupture bleeding(P=0.0475). Angiofibromas/fibrous cephalic plaque (155/186, 83.3%), subependymal nodules(103/146, 70.5%), lymphangioleiomyomatosis (102/157, 65.0%), hypomelanotic macules (114/186, 61.3%), and shagreen patch (83/186, 44.6%)were the most common clinical manifestations. All patients with lymphangioleiomyomatosis(LAM) were female(P < 0.0001).ConclusionsFemale patients predominated TSC-RAML patients. Most RAML were in stage 6. About 11.8% cases had a history of tumor rupture and hemorrhage. The higher grade meant the higher proportion of tumor rupture and hemorrhage. Dermatological and nervous lesions were the most common comorbidities. All patients with LAM in this study were female.

The optical microscope, scanning electron microscope, electron backscattered diffraction and three-dimensional atom probe techniques were employed to investigate the morphology, distributions of alloy elements and orientation of blocky α phase in β forging TC17 titanium alloy. The results show that the blocky α phases are distributed nearby the grain boundary, inside the prior β grains and recrystallized grains, while abnormal microstructure such as long and coarse α lamellar can also be observed inside the grains. There is no significant difference in the distribution of main elements between all kinds of abnormal α and β matrix phase. Therefore, the segregation of alloy composition does not cause the blocky α phase. The acicular α phase is randomly distributed in the grain and is acicular or cotton-blocky under the microscope. In addition, the orientation of α phase is also a main factor determining lamellar morphology. Coarse lamellar and agglomerate blocky α phase can be observed inside the fine recrystallized grains and slightly deformed grains. The fine recrystallized grains represent the undeformed or slightly deformed grains, which grain boundary α and α platelets inside the grain are basically single in orientation. Therefore, the α phase with the same orientation is easy to agglomerate under slow cooling. In addition to the dispersed cotton-blocky α phase, it also appears as blocky α phase which run through the entire grain or agglomerate near the grain boundary.

BackgroundThe persistently high prevalence of insomnia seriously affects the quality of life of all populations. Studies showed that state-trait anxiety， resilience and neuroticism are related to the occurrence of insomnia， while the research on the relationship among the four factors in college students is still insufficient.ObjectiveTo discuss the impact of neuroticism on insomnia among college students， and to examine the mediating role of state-trait anxiety and resilience in the relationship between neuroticism and insomnia， thus providing references for the intervention of insomnia in college students.MethodsFrom September to December 2020， simple random sampling techniques were utilized to select 1 416 students in a university in Sichuan province， and all subjects were assessed using State-Trait Anxiety Inventory （STAI）， Connor-Davidson Resilience Scale （CD-RISC）， Chinese Big Five Personality Inventory-15 （CBF-PI-15） and Insomnia Severity Index （ISI）. Then Pearson correlation analysis was conducted to examine the correlation among the above four scales， and the mediating role of STAI and CD-RISC in the relationship between CBF-PI-15 neuroticism dimension and ISI was verified by Process macro mediation analysis.ResultsInsomnia was reported in 241/1 416 （17.02%） college students. The prevalence rate of insomnia in male students was higher than that in female students， with statistical difference （χ2=16.417， P

ABSTRACTUnintended effects of gene edit crops may pose safety issues. Omics is a useful tool for researchers to evaluate these unexpected effects. Transcriptome and proteomics analyses were performed for two gene editors, CRISPR-Cas9 and adenine base editor (ABE) gene edit rice, as well as corresponding wild-type plants (Nipponbare). Transcriptome revealed 520 and 566 rice differentially expressed genes (DEGs) in the Cas9/Nip and ABE/Nip comparisons, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most DEGs participated in metabolism of terpenoids and polyketones, plant–pathogen interactions, and plant signal transduction. It mainly belongs to environmental adaptation. Proteomics revealed 298 and 54 rice differentially expressed proteins (DEPs) in the Cas9/Nip and ABE/Nip comparisons, respectively. KEGG pathway enrichment analysis showed that most DEPs participated in the biosynthesis of secondary metabolite and metabolic pathways.According to integrated transcriptomes and proteomics analysis, the results showed that no newly generated genes were identified as new transcripts of these differentially expressed genes, and gene edit tools had little effect on rice transcription levels and no new proteins were generated in the gene-edited rice.

Abstract Background Acute kidney injury (AKI) is a frequent complication of cardiac surgery that poses significant risks for both the development of chronic kidney diseases and mortality. Our previous study illustrated that heightened expression levels of faecal and plasma indole metabolites before the operation were associated with ischemic AKI. In this study, we aimed to validate the supposition that plasma indole-3-aldehyde (I3A) could serve as a predictive biomarker for AKI in patients undergoing cardiac surgery. Methods This statistical reanalysis utilized AKI metabolomic data from patients scheduled for cardiac surgery between April 2022 and July 2022 in two tertiary hospitals. Faecal and blood samples were prospectively collected before surgery within 24 h, and variables related to the preoperative, intraoperative, and postoperative periods were recorded. AKI diagnosis was based on the Kidney Disease Improving Global Outcomes criteria. Results In this study, 55 patients who underwent cardiac surgery were analyzed, and 27 of them (49.1%) developed postoperative AKI. Before surgery, these patients had significantly higher levels of faecal indole metabolites, including skatole, trans-3-indoleacrylic acid, and 5-methoxyindoleacetic acid. The plasma I3A, clinical model that considered perioperative and intraoperative variables, and their combination had area under the receiver operating characteristic curve (ROC) values of 0.79 (95% CI 0.67–0.91), 0.78 (95% CI 0.66–0.90), and 0.84 (95% CI 0.74–0.94) for predicting AKI, respectively. Furthermore, by utilizing net reclassification improvement and integrated discrimination improvement, plasma I3A showed significant improvements in risk reclassification compared to the clinical model alone. Conclusions The dysregulation of gut microbiota metabolism in patients scheduled for cardiac surgery can result in an increase in indoles from tryptophan metabolism, which may be associated with postoperative acute kidney injury (AKI). This suggests that indoles may serve as a predictive biomarker for AKI in patients undergoing cardiac surgery.

Spinal degenerative diseases are currently most common clinical conditions and also dominant diseases in the field of traumatology and orthopedics. Traditional Chinese manipulative therapy is a common approach for spinal degenerative diseases in traumatology and orthopedics of Chinese medicine. However, the current researches on the efficacy mechanism of traditional Chinese manipulative therapy is still not deep enough, which hinders the modernization and internationalization of the study on the manipulative therapy. Combination of medicine and industry is a novel research idea generated by the combination of relevant scientific and technological progress and the modernization of traditional Chinese medicine in recent years, which is helpful to realize the modernization of the research on the efficacy mechanism of traditional Chinese manipulative therapy and promote the internationalization of traditional Chinese manipulative therapy. Using the application status of modern engineering technologies such as mechanical engineering technology, software engineering technology, neural engineering technology and artificial intelligence technology in the research of traditional Chinese manipulative therapy as research ideas, this paper sorts out the researches on the modernization on the efficacy mechanism of traditional Chinese manipulation under the "combination of medicine and industry" research model in recent years, in order to provide reference ideas for the subsequent researches of the efficacy mechanism of manipulation in the treatment of spinal degenerative diseases.

Based on 40 years of routine water quality monitoring data from Shenzhen Bay and special intensive monitoring since 2021, we analysed the spatiotemporal variations in nitrogen and phosphorus pollution. The results indicate serious levels of nitrogen and phosphorus pollution in Shenzhen Bay. Although recent trends show a decline in concentrations of inorganic nitrogen and reactive phosphate—with the annual average concentration of inorganic nitrogen dropping below 1.0 mg/L since 2020 and reactive phosphate levels near the bay mouth decreasing to approximately 0.020 mg/L—the overall water quality still does not meet China’s Class IV seawater quality standards. Additionally, water quality is poorer during the rainy season, with the average summer concentration of inorganic nitrogen being over 25% higher than in spring and autumn. The water quality is primarily influenced by land-sourced pollutants and tidal effects, generally, with the concentration of DIN and DIP decreased from the estuary of the Shenzhen River, the main seagoing river, to the bay mouth. Near the river’s mouth, concentrations of inorganic nitrogen and reactive phosphate are approximately six and four times higher, respectively, than those near the bay mouth. Due to current emission standards and sewage disposal methods, the proportion of nitrate nitrogen in DIN has significantly increased, exceeding 80% since 2020. To improve the water quality of Shenzhen Bay and to reduce pollution inputs, coordinated land and marine development activities are recommended.

Although traditional machine learning based methods for predicting mine internal caused fire have certain predictive capabilities, they cannot effectively capture global dependencies between complex multivariate data, resulting in low prediction precision. In order to solve the above problems, a transformer based time series prediction method for mine internal caused fire is proposed. Firstly, the Hampel filter and Lagrange interpolation method are used to detect outliers and fill in missing values in the data. Secondly, the self attention mechanism of Transformer is utilized to extract features and predict trends from time series data. Finally, by adjusting the size and step size of the sliding window, the model is trained in different time dimensions at different time steps and prediction lengths. Combining gas analysis method, the iconic gases generated by mine fires (CO, O2, N2, CO2, C2H2, C2H4, C2H6) are used as input variables for the model, with CO as the target variable for model output and O2, N2, CO2, C2H2, C2H4, C2H6 as covariates for model input. Selecting the bundle data of S1206 return air corner fire warning in Ningtiaota Coal Mine of Shanmei Coal Group for experimental verification, the results show the following points. ① Univariate prediction and multivariate prediction of CO show that multivariate prediction has higher prediction precision than univariate prediction, indicating that multivariate prediction can improve the prediction precision of the model by capturing the correlation between sequences. ② When the time step is fixed, the prediction precision of the Transformer based mine internal caused fire prediction model decreases with the increase of prediction length. When the prediction length is fixed, the prediction precision of the model improves with the increase of time step. ③ The prediction accuracy of the Transformer algorithm is improved by 7.1%-12.6% and 20.9%-24.9% over the long short-term memory (LSTM) algorithm and recurrent neural network (RNN) algorithm, respectively.

BackgroundThe occurrence of acute kidney injury (AKI) following cardiac surgery is common and linked to unfavorable consequences while identifying it in its early stages remains a challenge. The aim of this research was to examine whether the fibrinogen-to-albumin ratio (FAR), an innovative inflammation-related risk indicator, has the ability to predict the development of AKI in individuals after cardiac surgery.MethodsPatients who underwent cardiac surgery from February 2023 to March 2023 and were admitted to the Cardiac Surgery Intensive Care Unit of a tertiary teaching hospital were included in this prospective observational study. AKI was defined according to the KDIGO criteria. To assess the diagnostic value of the FAR in predicting AKI, calculations were performed for the area under the receiver operating characteristic curve (AUC), continuous net reclassification improvement (NRI), and integrated discrimination improvement (IDI).ResultsOf the 260 enrolled patients, 85 developed AKI with an incidence of 32.7%. Based on the multivariate logistic analyses, FAR at admission [odds ratio (OR), 1.197; 95% confidence interval (CI), 1.064–1.347, p = 0.003] was an independent risk factor for AKI. The receiver operating characteristic (ROC) curve indicated that FAR on admission was a significant predictor of AKI [AUC, 0.685, 95% CI: 0.616–0.754]. Although the AUC-ROC of the prediction model was not substantially improved by adding FAR, continuous NRI and IDI were significantly improved.ConclusionsFAR is independently associated with the occurrence of AKI after cardiac surgery and can significantly improve AKI prediction over the clinical prediction model.

Abstract The severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne human-infecting bunyavirus, which utilizes two envelope glycoproteins, Gn and Gc, to enter host cells. However, the structure and organization of these glycoproteins on virion surface are not yet known. Here we describe the structure of SFTSV determined by single particle reconstruction, which allows mechanistic insights into bunyavirus assembly at near-atomic resolution. The SFTSV Gn and Gc proteins exist as heterodimers and further assemble into pentameric and hexameric peplomers, shielding the Gc fusion loops by both intra- and inter-heterodimer interactions. Individual peplomers are associated mainly through the ectodomains, in which the highly conserved glycans on N914 of Gc play a crucial role. This elaborate assembly stabilizes Gc in the metastable prefusion conformation and creates some cryptic epitopes that are only accessible in the intermediate states during virus entry. These findings provide an important basis for developing vaccines and therapeutic drugs.

Objective The sedimentary palaeogeomorphology of the Permian Qixia Formation in Sichuan Basin has key influence over the distribution and evolution of carbonate reservoirs. Restoring and characterizing the sedimentary palaeogeomorphology of Gaoshiti area can gain further understanding of the distribution of favorable sedimentary facies and predict the distribution of high-quality reservoirs. Methods Based on the division of the third-order sequence, the high-resolution sequence stratigraphic framework was established by using geochemical proxies, petrological markers and logging markers. Two kinds of sedimentary microfacies, i.e. beach core and beach wing, were also identified. In addition, this study has calculated the difference of layer thicknesses in the sequence, and has identified different types of sedimentary sequences. A number of values including the beach core/beach wing value, Fe/Mn and MgO/Al2O3 ratios and other geochemical parameters were also calculated. The characteristics of the above parameters on the plane are analyzed and re-evaluated, which are all used to restore the palaeogeomorphic characteristics of the sedimentary period of the Qixia Formation in the study area. Results The result of research shows that the palaeogeomorphic characteristics of the 1st Member of Qixia are higher in the northwest and south, lower in the middle, and the palaeogeomorphic drop is obvious. Our new results show that the palaeogeomorphic highlands of the 2nd Member of Qixia are distributed in the middle of the study area, that the western palaeogeomorphology is low, and that the overall difference of palaeogeomorphology is small. Conclusion According to the results of ancient landform restoration, Gaoshiti-Moxi area forms a shallow water ring zone in the northeast-southwest direction where granular shoals are developed. Local micro-positive structures and granular shoals are developed in the south. However, the distribution of micro-positive structures is so limit that the extension range of granular shoals is limit. This study highlights that future exploration and development should focus on the development area of granular shoals in the northeast-southwest direction.

In this note, we establish the global C3,α{C}^{3,\alpha } regularity for potential functions in optimal transportation between hypercubes in Rn{{\mathbb{R}}}^{n} for n≥3n\ge 3. When n=2n=2, the result was proved by Jhaveri. The C3,α{C}^{3,\alpha } regularity is also optimal due to a counterexample in the study by Jhaveri.

In the process of applying deep reinforcement learning (DRL) to realize autonomous behavioral decision-making of robotic arms, the high-dimensional continuous state-action space is prone to low data sampling efficiency and low quality of empirical samples, which ultimately leads to slow convergence of the reward function and long learning time. To address this problem, a DRL model that introduces prior knowledge was proposed. The model was combined with the inverse kinematics of the robotic arm, and prior knowledge was introduced to guide the agent during the sampling phase of DRL, addressing the issues of low data sampling efficiency and poor quality of experience samples during the learning process. Furthermore, the introduced prior knowledge DRL model's strong generalization capabilities were verified when facing new tasks through network parameter transfer. Lastly, joint simulation experiments were conducted using Python and the CoppeliaSim platform. The results show that the DRL model with the introduction of prior knowledge improves the learning efficiency by 13.89% and 12.82%, and the success rate of completing the task increases by 16.92% and 13.25% than the original model; in the new task, the learning rate improves by 23.08% and 23.33%, and the success rate improves by 10.7% and 11.57%.

This study aimed to investigate the effect of diatrizoate vaginal stump markers on the dose distribution of postoperative radiotherapy for cervical cancer. From July 2022 to July 2023, 12 patients who underwent radical postoperative radiotherapy for cervical cancer at Gansu Provincial Maternity and Child-Care Hospital, Gansu Provincial Central Hospital, and who had cotton balls with meglumine diatrizoate placed to mark the vaginal stump before positioning were enlisted. Volume modulated arc therapy was designed as Plan A and was used in the actual treatment plan. The relative electron density value of the vaginal stump area marked by meglumine diatrizoate was replaced by the relative electron density value of the normal vaginal wall, and Plan B was recalculated, keeping the planning optimization parameters unchanged. The evaluation indicators and dose distributions were compared between the two group. The results showed that the Homogeneity index（HI）was slightly worse in the Plan B group than the Plan A group (1.048 vs. 1.047) but the Conformance index (CI) (0.870 vs. 0.868) was better in the Plan B group. The average dose to the planned target volum (46.48 vs. 46.47) and the dose to the vaginal stump (47.14 vs. 46.45) were higher for Plan B than Plan A, and the minimum dose to the rectum (6.42 vs. 6.49) was lower for Plan B than Plan A. The effect of diatrizoate vaginal stump markers on dose distribution was negligible.

This study uses the finite element method (FEM) to investigate the effect of key structural parameters on the impact resistance of E-glass 3D orthogonal woven (3DOW) composites subjected to low-velocity impact. These structural parameters include the number of y-yarn layers, the path of the binder yarn (z-yarn), and the density of the x-yarn. Using ABAQUS, yarn-level finite element (FE) models are created based on the measured geometrical parameters and validated for energy absorption and damage behavior from experimental data gathered from the previous study. The results from finite element analysis (FEA) indicate that the x-yarn density and the binder path substantially influenced the composites’ damage behavior and impact performance. Increasing x-yarn density in 3DOW leads to a 15% increase in energy absorption compared to models with reduced x-yarn densities. Moreover, as the x-yarn density increases, crack lengths at the back face of the resin matrix decrease in the y-yarn direction but increase in the x-yarn direction. The basket weave structure absorbs less energy than plain and 2 × 1 twill structures due to the less constrained weft primary yarns. These results underscore the importance of these structural parameters in optimizing 3DOW composite for better impact performance, providing valuable insights for the design of advanced composite structures.

In order to solve the existing problems such as non-standard size and inconvenient activities of the arm of dress form in current market, based on the latest collection of young male body size data, the forward and reverse modeling technology were used to build arm model. And two kinds of shoulder joint fittings were designed. The products were manufactured by rapid prototyping technology to realize free rotation of the arm at 90° coronal plane and 360° sagittal plane, which can meet the needs of draping fixation, and provide the necessary tools for garment sleeve design. The subjective and objective evaluation compared results show that the comprehensive performance of product with damping hinge by using forward modeling technology was better. In order to further improve the modeling effect, the forward and reverse hybrid modeling technology is suggested in the modeling building of the arm of dress form. This method provides technical reference for related production enterprises.

In recent years, with advancements in machine control automation and intelligent systems, both domestic and international research has increasingly focused on the automation and semi-automation of excavator control. This study introduces an auxiliary control system for excavator fleets, which utilizes sensor and wireless communication technologies. The research investigates the excavation errors that arise when operators rely on personal vision and experience in complex working environments, as well as the challenges of managing large fleets of excavators. By assisting operators in controlling the excavators, the system significantly reduces the operator’s experience requirements and work intensity and provides higher precision, consistency, and efficiency for excavation equipment. This method not only further improves the operating efficiency and excavation accuracy, but also saves the overall construction cost and improves the sustainability of the project.

Objective: This paper discusses the feasibility of using mineral element fingerprinting technology of to identify tobacco producing areas, screens out the effective indicators, and constructs the discrimination model of tobacco producing areas. Methods: The contents of 20 mineral elements in tobacco leaves from 11 producing areas were simultaneously determined by ICP-MS, and the data were analyzed by variance analysis, cluster analysis, principal component analysis and discriminant analysis. Results: The contents of 16 elements were significantly different among producing areas, and principal component analysis resulted in 6 principal components, with the cumulative variance contribution of 89.253%. Using linear discriminant analysis, four elementsmineral, K, Mn, Se and Ba, were screened as the effective indicators to discriminate the geographical origin of tobacco leaves. The established discriminant model could accurately distinguish tobacco leaf samples from different tobacco producing areas. Conclusion: Significant differences were showed in the contents of mineral elements in tobacco leaves from 11 producing areas. Four elements, K, Mn, Se and Ba, are important indicators for distinguishing tobacco producing areas. Mineral element fingerprinting technology can be used for distinguishing tobacco producing areas.

To meet the needs of flight teaching and training, aiming at the interactive, multi-input multi-output and comprehensive sequential logic controlling characteristic of integrated avionics system display and control interface for fighters, a functional simulation method by designing display and control logic independently is introduced. On the basis of this structure, a loosely coupled, modular and visual simulation method of display and control interface for multi-function fire control radar is presented, and the realization of display and control logic based on Simulink/Stateflow is introduced. The application result shows that the program progress is more intuitive, and the code is more maintainable, so this design method can improve the fidelity and developing efficiency of display and control simulation. All these researches can provide technology reference for the simulation of display and control interfaces for avionics systems and its subsystems.

In the process of coal mining, the coal body is subjected to external force, resulting in pore penetration and strength decline, which is easy to cause engineering disasters such as roadway surrounding rock instability and roof collapse. In order to study the correlation and influence mechanism between pore distribution and strength characteristics, three groups of representative coal samples （N22, H52 and H42 coal samples） from Yushenfu Mining Area in northern Shaanxi Province of China were selected to carry out the pore size distribution and strength characteristics tests, and the results were analyzed based on Pearson correlation coefficient and energy evolution. The research conclusions are as follows: the three groups of coal samples are mainly developed with micropores and transition pores, and the development of macropores is extremely poor. The compressive strength of N22 coal sample is the highest, which is 31.3 MPa, followed by H52 and H42 coal samples, which are 14.2 MPa and 12.5 MPa, respectively. The total porosity of coal samples and the porosity of large pores, middle pores and transition pores are negatively correlated with compressive strength, and the porosity of micropores is positively correlated with compressive strength. During the compression process, the micropore is beneficial to the accumulation of elastic strain energy Ue during the compression of coal samples, and to a certain extent, it is beneficial to improve the compressive capacity of coal samples. Large pores, middle pores and transition pores consume dissipation energy Ud and form cracks through each other.

In order to study the dynamic evolution law of the pressure relief gas reservoir in the mining of extra-thick coal seams, the 3DEC numerical simulation software was used to analyze the stress and displacement distribution law of the overburden rock caused by mining, and the evolution characteristics of the pressure relief gas reservoir were studied, and the pressure relief gas was proposed. The method of discriminating the location of the reservoir area, and the practice of gas drainage in high-position boreholes at the test working face. The results show that: after the extra-thick coal seam is mined, the height of the overburden caving zone is 49.5 m, and the height of the fracture zone is 104 m. After the key strata in the pressure relief zone on the working face side loses stability, a pressure relief gas reservoir is formed below it. Pressure relief gas reservoir areas are divided into three categories: ①The key layer is in the bent subsidence zone, and the high-level reservoir is formed below it; ②The key layer is in the fracture zone, and the middle-level reservoir is formed under the masonry beam; ③The key layer is in the fracture zone. In the collapse zone, a low-level storage area is formed under the cantilever beam. The shape and area of ​​the reservoir are closely related to the state of the control key layer above. Before the control key layer is broken, the area continues to increase. The shape of the low-level reservoir is rectangular, and the shape of the middle and high-level reservoir is semi-elliptical, expanding to a three-dimensional shape. It is a rectangular cross-section ring body and an elliptical cross-section semi-ellipsoid body; the area decreases rapidly after breaking, and then changes periodically with the pressure of the overlying rock. The low-level reservoir is trapezoidal, the middle-level reservoir is triangular, and the high-level reservoir is shaped like a trapezoid. The shape is a semi-ellipse, and the three-dimensional shape is expanded to a rectangular cross-section ring, a triangular cross-section ring and an elliptical cross-section semi-ellipsoid. The location of the final hole of the high-level borehole was arranged in the range of the middle reservoir area, and the drainage verification was carried out. The maximum drainage concentration of a single hole was 34.5%, the average drainage concentration was 16.8%, and the upper corner gas concentration after drainage was 0.55%, The gas drainage effect is good, indicating that the arrangement of high-level drilling holes according to this method is reasonable.

In order to solve the problems of low detection accuracy and less attention of vairous malicious traffic identification in traditional intrusion detection system (IDS) in the face of massive data mixed with various malicious traffic, an intrusion detection model based on capsule network CapIDS was proposed. In this model, the traffic to be measured was input into the capsule network in the form of capsules, and the characteristics of malicious traffic were extracted by using the dynamic routing mechanism to complete the identification of malicious traffic. At the same time, the structure of the capsule network was improved, which made the model have stronger generalization ability and improved the detection accuracy of malicious traffic. The experimental results show that the detection accuracy of the proposed model for malicious traffic has reached 99.50% on the NSL-KDD data set, which is better than that of other schemes.

Iron metabolism is considered to play the principal role in sepsis, but the key iron metabolism-related genetic signatures are unclear. In this study, we analyzed and identified the genetic signatures related to the iron-metabolism in sepsis by using a bioinformatics analysis of four transcriptomic datasets from the GEO database. A total of 21 differentially expressed iron metabolism-related signatures were identified including 9 transporters, 8 enzymes, and 4 regulatory factors. Among them, lipocalin 2 was found to have the highest diagnostic value as its expression showed significant differences in all the comparisons including sepsis vs healthy controls, sepsis vs non-sepsis diseases, and mild forms vs severe forms of sepsis. Besides, the cytochrome P450 gene CYP1B1 also showed diagnostic values for sepsis from the non-sepsis diseases. The CYP4V2, LTF, and GCLM showed diagnostic values for distinguishing the severe forms from mild forms of sepsis. Our analysis identified 21 sepsis-associated iron metabolism-related genetic signatures, which may represent diagnostic and therapeutic biomarkers of sepsis, and will improve our understanding of the molecular mechanism underlying the occurrence of sepsis.

Water, in ultra-deep layers of the earth and in layers receiving abnormally high heat, can exist in a supercritical state. Supercritical water (SCW) can participate in the transformations of organic compounds not only as a solvent but also as a reactant, influencing petroleum formation and the evolution of sedimentary organic matter. Here, we carried out hydrous pyrolysis experiments in both SCW and water vapor (WV) using two organic-rich marine rocks under closed conditions, to quantitatively evaluate the generation potential of hydrocarbons in a supercritical state and to clarify the effect of water phase on hydrous pyrolysis experiments. The results showed that SCW promoted gaseous and liquid hydrocarbon generation and facilitated the cracking of aliphatic hydrocarbons. For gaseous hydrocarbons, the action of SCW became stronger as the temperature increased. For liquid hydrocarbons, the peak yields of bitumen were enhanced by the SCW, and the temperature corresponding to peak yield in SCW was lower than that in WV. These results were attributed to the supply of hydrogen and oxygen from SCW for the petroleum formation and cracking. The δ13C and δD values of gases were also influenced by SCW. However, these values obtained in SCW did not always become positive as their yields increased. Generally, methane (CH4) was enriched in 12C and 1H. The influence of SCW on the isotopic fractionation of ethane (C2H6) and propane (C3H8) was more complex. The water phase is an important factor affecting the experimental results of hydrous pyrolysis. From these findings, it can be concluded that SCW allowed for increased conversion of sedimentary organic matter to gaseous and liquid hydrocarbons in ultra-deep layers and layers affected by volcanic-hydrothermal activity.

This study investigates the effects of the number of layers, x-yarn (weft) density, and z-yarn (binder) path on the mechanical behavior of E-glass 3D orthogonal woven (3DOW) composites during low-velocity impacts. Meso-level finite element (FE) models were developed and validated for 3DOW composites with different yarn densities and z-yarn paths, providing analyses of stress distribution within reinforcement fibers and matrix, energy absorption, and failure time. Our findings revealed that lower x-yarn densities led to accumulations of stress concentrations. Furthermore, changing the z-yarn path, such as transitioning from plain weaves to twill or basket weaves had a noticeable impact on stress distributions. The research highlights the significance of designing more resilient 3DOW composites for impact applications by choosing appropriate parameters in weaving composite designs.

Nipah virus (NiV) is a highly lethal zoonotic virus with a potential large-scale outbreak, which poses a great threat to world health and security. In order to explore more potential factors associated with NiV, a proximity labeling method was applied to investigate the F, G, and host protein interactions systematically. We screened 1996 and 1524 high-confidence host proteins that interacted with the NiV fusion (F) glycoprotein and attachment (G) glycoprotein in HEK293T cells by proximity labeling technology, and 863 of them interacted with both F and G. The results of GO and KEGG enrichment analysis showed that most of these host proteins were involved in cellular processes, molecular binding, endocytosis, tight junction, and other functions. Cytoscape software (v3.9.1) was used for visual analysis, and the results showed that Cortactin (CTTN), Serpine mRNA binding protein 1 (SERBP1), and stathmin 1 (STMN1) were the top 20 proteins and interacted with F and G, and were selected for further validation. We observed colocalization of F-CTTN, F-SERBP1, F-STMN1, G-CTTN, G-SERBP1, and G-STMN1 using confocal fluorescence microscopy, and the results showed that CTTN, SERBP1, and STMN1 overlapped with NiV F and NiV G in HEK293T cells. Further studies found that CTTN can significantly inhibit the infection of the Nipah pseudovirus (NiVpv) into host cells, while SERBP1 and STMN1 had no significant effect on pseudovirus infection. In addition, CTTN can also inhibit the infection of the Hendra pseudovirus (HeVpv) in 293T cells. In summary, this study revealed that the potential host proteins interacted with NiV F and G and demonstrated that CTTN could inhibit NiVpv and HeVpv infection, providing new evidence and targets for the study of drugs against these diseases.

The surface spike (S) glycoprotein mediates cell entry of SARS-CoV-2 into the host through fusion at the plasma membrane or endocytosis. Omicron lineages/sublineages have acquired extensive mutations in S to gain transmissibility advantages and altered antigenicity. The fusogenicity, antigenicity, and evasion of Omicron subvariants have been extensively investigated at unprecedented speed to align with the mutation rate of S. Cells that overexpress receptors/cofactors are mostly used as hosts to amplify infection sensitivity to tested variants. However, systematic cell entry comparisons of most prior dominant Omicron subvariants using human lung epithelium cells are yet to be well-studied. Here, with human bronchial epithelium BEAS-2B cells as the host, we compared single-round virus-to-cell entry and cell-to-cell fusion of Omicron BA.1, BA.5, BQ.1.1, CH.1.1, XBB.1.5, and XBB.1.16 based upon split NanoLuc fusion readout assays and the S-pseudotyped lentivirus system. Virus-to-cell entry of tested S variants exhibited cell-type dependence. The parental Omicron BA.1 required more time to develop full entry to HEK293T-ACE2-TMPRSS2 than BEAS-2B cells. Compared to unchanged P681, S-cleavage constructs of P681H/R did not have any noticeable advantages in cell entry. Omicron BA.1 and its descendants entered BEAS-2B cells more efficiently than D614G, and it was slightly less or comparable to that of Delta. Serine protease-pretreated Omicron subvariants enhanced virus-to-cell entry in a dose-dependent manner, suggesting fusion at the plasma membrane persists as a productive cell entry route. Spike-mediated cell-to-cell fusion and total S1/S2 processing of Omicron descendants were similar. Our results indicate no obvious entry or fusion advantages of recent Omicron descendants over preceding variants since Delta, thus supporting immune evasion conferred by antigenicity shifts due to altered S sequences as probably the primary viral fitness driver.

In order to study the water infiltration and self-weight collapse deformation characteristics of Jingyuan loess with large thickness under the condition of immersion, a field immersion test without water injection holes was carried out in the self-weight collapsed loess site of Jingyuan North Station along the Zhongwei−Lanzhou Railway. The surface and underground collapsible deformation, cracks, water content and vertical stress in the soil around the test pit were monitored and analyzed. The water diffusion, self-weight collapsible characteristics and vertical stress in the soil were studied, and the regional correction coefficient β0 value and wetting angle were discussed. The results showed that: the change of volumetric water content was divided into four stages: immersion stabilization (two), rapid increase (one) and slow increase (one). In the immersion process, the vertical infiltration of water was accelerated and the radial diffusion was slowed down at 21 m, and the final shape of the wetting front was presented as elliptical. According to the water content test results of exploratory wells and boreholes, the maximum wetting angle was calculated to be 41°. The self-weight collapse process of loess in the site went through three stages: severe collapse, slow collapse and consolidation stabilization. At the end of the test, a total of 13 ring cracks were developed, and the farthest point of the cracks was 26 m from the edge of the test pit. According to the laboratory test and field test results, it was suggested that the regional correction coefficient should be corrected along the depth of the soil layer, and the β0 value was taken as 1.05 within 0−10 m and 0.95 within 10−27 m. In the depth range from the surface to 21 m, the foundation soil was saturated and fully collapsed. The vertical stress in the soil increased linearly along the depth, and the vertical stress in the soil was close to the saturated self-weight stress. The foundation soil below 21 m failed to collapse entirely, and the vertical stress in the soil decreased gradually. The research results could be applied to the later construction of Zhongwei−Lanzhou Railway and provide a reference for other regional engineering projects.

Pancreatic cancer is the sixth leading cause of cancer-related death in China. It is characterized by high malignancy and concealment at early stages. More than 80% of patients have progressed to advanced stages at the time of diagnosis, and missed the opportunity of surgical resection. Pancreatic cancer patients who have successfully received surgical resection are at a high risk of metastasis, and the 5-year survival rate is only 15%-20%. Unlike other types of cancer, radiotherapy, chemotherapy and immunotherapy do not work well for pancreatic cancer, and the research in molecular subtyping and precision medical therapy technology is also slow. It is an urgent need to devote more clinical medical resources and transfer high-quality scientific research achievements to improve the prognosis of patients. The year 2022 is a year of profound accumulation in the field of translational pancreatic cancer research, witnessing the generation of the first small-molecule drug targeting KRASG12D and the clinical application of personalized T-cell receptor engineered T (TCR-T) cell therapy, as well as other advances in basic fields such as pathogenesis, molecular mechanism, metabolic immune microenvironment, and clinical fields such as early diagnosis, surgical treatment, radiation therapy, chemotherapy and immunotherapy. Here we reviewed the latest progress in research, diagnosis and treatment of pancreatic cancer in 2022.

In this study, 1H magnetic resonance spectroscopy (1H MRS) was used to analyze metabolites in the hippocampus of chronic type 1 diabetes mellitus (T1DM) rats induced by streptozocin and T1DM rats treated with long-term insulin. The results showed that, compared with control rats and insulin-treated T1DM rats, the fasting blood glucose of T1DM rats increased significantly, while the body weight of T1DM rats decreased remarkably (p < 0.05). The contents of myo-inositol (Ins), taurine (Tau) and glutamate (Glu) of T1DM rats were significantly higher than those of control rats (p=0.000, p=0.003, p=0.014, respectively), and the contents of Ins and Tau of insulin-treated T1DM rats were significantly decreased than those in the T1DM diabetic rats (p=0.000, p=0.010, respectively). Contents of Glu and Glx (Glu and glutamine) of insulin-treated T1DM rats were significantly higher than those in the control group (p=0.007, p=0.042, respectively). The metabolite changes of Ins and Tau in hippocampus of T1DM rats are sensitive to insulin treatment.

ObjectiveTo observe the intervention effect of Shuganjieyu Formula on the rat model of constipation irritable bowel syndrome (IBS-C) and its effects on 5- hydroxytryptamine (5-HT), corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH), and to explore their mechanism of regulating brain-gut axis function.MethodsTotally fourty rats were randomly divided into four groups with 10 rats in each group, including normal control group, model group, low (5 mg/kg) and high (20 mg/kg) doses of Shuganjieyu Formula groups. Except for the normal control group, the other three groups of rats were gavaged with ice water for preparing the IBS-C model. Subsequently, the low and high doses groups of rats were treated with Shuganjieyu Formula for 2 weeks via oral gavage. The body weight of rats, total particle number and water content of fecal in the four groups were observed for 28 days. At last, serum of inferior vena cava and colon were collected from rats after anesthesia of 3% pentobarbital sodium. The morphological changes of colon tissue and the count of mast cells (MC) were observed with light microscope. Immunohistochemistry was used to detect the positive expression of 5-HT in the colon of rats in each group. The serum levels of 5-HT, CRH and ACTH were detected by ELISA.ResultsCompared with the normal control group, the body weight of rats in IBS-C model group increased slowly, the number of fecal grains and the fecal water content decreased (P

Abstract Background Research on aging is growing as the elderly make up a greater share of the population, focusing on reversing and inhibiting the aging process. The exhaustion and senescence of stem cells are the fundamental drivers behind aging. β-Carotene has been depicted to have many biological functions, and we speculate that it may have an anti-aging effect. Methods Firstly, the anti-aging property of β-carotene was investigated in vitro using mesenchymal stem cells (MSCs) induced by H2O2. The anti-aging effect was characterized using Western-bloting, confocal laser scanning microscopy, indirect immunofluorescence, and immunohistochemistry. The anti-aging property was also tested in vivo using aged mice. Results The in vitro experiment revealed that β-carotene could relieve the aging of MSCs, as evidenced by a series of aging marker molecules such as p16 and p21. β-Carotene appeared to inhibit aging by regulating the KAT7-P15 signaling axis. The in vivo experiment revealed that β-carotene treatment has significantly down-regulated the aging level of tissues and organs. Conclusions In this work, we explored the anti-aging effect of β-carotene in vivo and in vitro. The experimental results indicate that β-carotene may be an important potential anti-aging molecule, which can be used as a drug or in functional food to treat aging in the future.

As one of the essential means to support the operation of new power systems in the future， demand-side flexible resources can effectively deal with the challenges brought about by the integration of high-proportion renewables and clean energies. A scientific， rational， and perfect ancillary service market mechanism is significant in promoting the utilization and development of demand-side flexible resources. Therefore， this paper investigates and summarizes the current research and market practice of auxiliary service market mechanisms at home and abroad. Firstly， the existing domestic ancillary service market mechanisms for demand-side flexible resources participating in auxiliary service market trading are sorted out， and several problems existing in the operation of the current market mechanism are summarized. Then， the relevant market mechanisms of Australia， the United States， and the United Kingdom and their exploration and practice in flexible resource utilization on the demand side are introduced. Finally， future suggestions on the utilization of flexible resources on demand side and the construction of power ancillary service market in China are advanced.

This study presented a mathematical description of an arbitrary shape area, and after analyzing the characteristics of the near-polar orbital co-ground trajectory constellation that satisfies the revisit interval constraint, an optimization model of the orbital parameters design of reference satellites in regional coverage constellations was constructed. Then based on the designed area covering the constellation of common ground trajectories, a simple and effective two-pulse maneuvering scheme was adopted for the needs of sudden tasks, and an existing satellite scheduling optimization model using the golden section method to constrain the boundary of substellar point coverage was constructed. Finally, the optimal design of regional coverage constellations and existing satellite dispatches was carried out for the sea areas with the most frequent tropical cyclone activities in the northwest Pacific Ocean, and the simulation results verified the effectiveness of the proposed optimization model and have certain practical use value.

ObjectiveTo analyze the research status of forensic medicine in China from 2010 to 2019, obtain the development trend of forensic medicine and explore the hotspots and research frontiers.MethodsThe forensic medical academic papers published on China National Knowledge Infrastructure （CNKI） database from 2010 to 2019 were collected. CiteSpace 5.7.R1, an information visualization analysis software, was used to analyze publication organizations, authors, keywords, and other elements.ResultsThe majority of the research institutions were universities, provincial and ministerial scientific research and forensic institutions. Forensic pathology was still an important branch of forensic medicine and a popular research direction. The “polymorphism” and “Y chromosome” had been the research hotspots in recent years. “Medical damage” and “standard” were the most novel studies.ConclusionIn order to provide scientific basis and research direction for forensic research, this paper analyzes the cooperation network, research hotspots and research innovation in forensic research.

Visual event-related potential （ERP） is an electrophysiological technique that objectively reflects the cognitive processing of stimulus from the perspective of detecting and recording neural electrophysiology responses using different paradigms of visual stimuli. Its endogenous components are closely related to advanced psychological activities. This article introduces the characteristics of main endogenous components including visual mismatch negativity （vMMN）, N200 and P300, reviews the research progress of visual ERP in the sequelae of brain injury and objective evaluation of visual function, and prospects the application prospect of visual ERP in the field of forensic medicine.