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Copyright of Baghdad Science Journal is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this research, an Artificial Bee Colony (ABC) algorithm based Selective Harmonics Elimination (SHE) technique is used as a pulse generator in a reduced switch fifteen level inverter that receives input from a PV system. Pulse width modulation based on Selective Harmonics Elimination is mostly used to suppress lower-order harmonics. A high gain DC-DC-SEPIC converter keeps the photovoltaic (PV) panel's output voltage constant. The Grey Wolf Optimization (GWO) filter removes far more Photovoltaic panel energy from the sunlight frame. To eliminate voltage harmonics, this unique inverter architecture employs a multi-carrier duty cycle, a high-frequency modulation approach. The proposed ABC harmonics elimination approach is compared to SHE strategies based on Particle Swarm Optimization (PSO) and Flower Pollination Algorithm (FPA). The suggested system's performance is simulated and measured using the MATLAB simulation tool. The proposed ABC approach has a THD level of 4.86%, which is better than the PSO and FPA methods. [ABSTRACT FROM AUTHOR]

To eliminate the usage of salt, salt-free reactive dyeing using CHPTAC (3-Chloro-2-hydroxypropyl trimethylammonium chloride) has been proposed. Studies have shown promising results, such as better dye shade attainment and less effluent load compared to conventional methods. In this work, to improve the effective utilisation of CHPTAC, the cotton fabric has been treated with cellulase enzyme and the application of CHPTAC is carried out using the ultrasonication technique. The samples are then characterised for colour parameters and it is found that the enzymatic treatment and ultrasonication lead to improve the dye uptake, as the interaction of CHPTAC with the fabric becomes better. [ABSTRACT FROM AUTHOR]

Data Mining is a powerful technology and is used to identify useful and understandable patterns by analyzing large sets of data. It gives a detailed view of various disease predictions. It will be more useful especially in pandemic times. During these days, doctors are in the front line and battling with the COVID-19 virus. It will be hard for people to immediately get medical guidance or appointments. Our proposed system, the smart health application will come in handy at these times. The system allows people to get medical guidance for their health issues. Also, the system is fed with symptoms and the disease-related with it which will give high accuracy for disease prediction. Our model aims to use Stacking Ensemble Classification Algorithms to give high accuracy and correct prediction than Naive Bayes, Random Forest, Support Vector Machine, K -- Nearest Neighbor, Decision Tree, Logistic Regression for different types of 149 diseases. The GUI is designed which can be used easily to predict the different types of disease accurately. [ABSTRACT FROM AUTHOR]

Neuroblastoma (NB) is a heterogeneous cancer of the sympathetic nervous system, which accounts for 7-10% of paediatric malignancies worldwide. Due to the lack of targetable molecular aberrations in NB, most treatment options remain relatively nonspecific. Here, we investigated the therapeutic potential of BCI, an inhibitor of DUSP1 and DUSP6, in cultured NB cells. BCI was cytotoxic in a range of NB cell lines and induced a short-lived activation of the AKT and stress-inducible MAP kinases, although ERK phosphorylation was unaffected. Furthermore, a phosphoproteomic screen identified significant upregulation of JNK signalling components and suppression in mTOR and R6K signalling. To assess the specificity of BCI, CRISPR-Cas9 was employed to introduce insertions and deletions in the DUSP1 and DUSP6 genes. Surprisingly, BCI remained fully cytotoxic in NB cells with complete loss of DUSP6 and partial depletion of DUSP1, suggesting that BCI exerts cytotoxicity in NB cells through a complex mechanism that is unrelated to these phosphatases. Overall, these data highlight the risk of using an inhibitor such as BCI as supposedly specific DUSP1/6, without understanding its full range of targets in cancer cells. [ABSTRACT FROM AUTHOR]

IntroductionBioassembly techniques for the application of scaffold-free tissue engineering approaches have evolved in recent years toward producing larger tissue equivalents that structurally and functionally mimic native tissues. This study aims to upscale a 3-dimensional bone in-vitro model through bioassembly of differentiated rat osteoblast (dROb) spheroids with the potential to develop and mature into a bone macrotissue.MethodsdROb spheroids in control and mineralization media at different seeding densities (1 × 104, 5 × 104, and 1 × 105 cells) were assessed for cell proliferation and viability by trypan blue staining, for necrotic core by hematoxylin and eosin staining, and for extracellular calcium by Alizarin red and Von Kossa staining. Then, a novel approach was developed to bioassemble dROb spheroids in pillar array supports using a customized bioassembly system. Pillar array supports were custom-designed and printed using Formlabs Clear Resin® by Formlabs Form2 printer. These supports were used as temporary frameworks for spheroid bioassembly until fusion occurred. Supports were then removed to allow scaffold-free growth and maturation of fused spheroids. Morphological and molecular analyses were performed to understand their structural and functional aspects.ResultsSpheroids of all seeding densities proliferated till day 14, and mineralization began with the cessation of proliferation. Necrotic core size increased over time with increased spheroid size. After the bioassembly of spheroids, the morphological assessment revealed the fusion of spheroids over time into a single macrotissue of more than 2.5 mm in size with mineral formation. Molecular assessment at different time points revealed osteogenic maturation based on the presence of osteocalcin, downregulation of Runx2 (p < 0.001), and upregulated alkaline phosphatase (p < 0.01).DiscussionWith the novel bioassembly approach used here, 3D bone macrotissues were successfully fabricated which mimicked physiological osteogenesis both morphologically and molecularly. This biofabrication approach has potential applications in bone tissue engineering, contributing to research related to osteoporosis and other recurrent bone ailments.