9 results on '"Tsai-Yuan, Chen"'
Search Results
2. The gephyrin scaffold modulates cortical layer 2/3 pyramidal neuron responsiveness to single whisker stimulation
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Tsai, Yuan-Chen, Hleihil, Mohammad, Otomo, Kanako, Abegg, Andrin, Cavaccini, Anna, Panzanelli, Patrizia, Cramer, Teresa, Ferrari, Kim David, Barrett, Matthew J. P., Bosshard, Giovanna, Karayannis, Theofanis, Weber, Bruno, Tyagarajan, Shiva K., and Stobart, Jillian L.
- Published
- 2024
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3. A genetically encoded sensor for in vivo imaging of orexin neuropeptides
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Duffet, Loïc, Kosar, Seher, Panniello, Mariangela, Viberti, Bianca, Bracey, Edward, Zych, Anna D., Radoux-Mergault, Arthur, Zhou, Xuehan, Dernic, Jan, Ravotto, Luca, Tsai, Yuan-Chen, Figueiredo, Marta, Tyagarajan, Shiva K., Weber, Bruno, Stoeber, Miriam, Gogolla, Nadine, Schmidt, Markus H., Adamantidis, Antoine R., Fellin, Tommaso, Burdakov, Denis, and Patriarchi, Tommaso
- Published
- 2022
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4. Modulation of sleep/wake patterns by gephyrin phosphorylation status.
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Tsai, Yuan‐Chen, ElGrawani, Waleed, Muheim, Christine, Spinnler, Andrea, Campbell, Benjamin F. N., Lasic, Denis, Hleihil, Mohammad, Brown, Steven A., and Tyagarajan, Shiva K.
- Subjects
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SLEEP duration , *SCAFFOLD proteins , *EYE movements , *NEURAL transmission , *SLEEP , *WAKEFULNESS , *SLEEP-wake cycle - Abstract
Sleep/wake cycles intricately shape physiological activities including cognitive brain functions, yet the precise molecular orchestrators of sleep remain elusive. Notably, the clinical impact of benzodiazepine drugs underscores the pivotal role of GABAergic neurotransmission in sleep regulation. However, the specific contributions of distinct GABAA receptor subtypes and their principal scaffolding protein, gephyrin, in sleep dynamics remain unclear. The evolving role of synaptic phospho‐proteome alterations at excitatory and inhibitory synapses suggests a potential avenue for modulating gephyrin and, consequently, GABAARs for sleep through on‐demand kinase recruitment. Our study unveils the distinctive roles of two prevalent GABAA receptor subtypes, α1‐ and α2‐GABAARs, in influencing sleep duration and electrical sleep activity. Notably, the absence of α1‐GABAARs emerges as central in sleep regulation, manifesting significant alterations in both non‐rapid eye movement (NREM) and rapid eye movement (REM) sleep during dark or active phases, accompanied by altered electroencephalogram (EEG) patterns across various frequencies. Gephyrin proteomics analysis reveals sleep/wake‐dependent interactions with a repertoire of known and novel kinases. Crucially, we identify the regulation of gephyrin interaction with ERK1/2, and phosphorylations at serines 268 and 270 are dictated by sleep/wake cycles. Employing AAV‐eGFP‐gephyrin or its phospho‐null variant (S268A/S270A), we disrupt sleep either globally or locally to demonstrate gephyrin phosphorylation as a sleep regulator. In summary, our findings support the local cortical sleep hypothesis and we unveil a molecular mechanism operating at GABAergic synapses, providing critical insights into the intricate regulation of sleep. [ABSTRACT FROM AUTHOR]
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- 2024
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5. The behavioural response of mice lacking NK1 receptors to guanfacine resembles its clinical profile in treatment of ADHD
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Pillidge, Katharine, Porter, Ashley J, Dudley, Julia A, Tsai, Yuan-Chen, Heal, David J, and Stanford, Clare S
- Published
- 2014
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6. An examination of the wind-driven effect on the drift of precipitation particles using the Chung-Li VHF radar
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Tsai Yuan Chen, Yen Hsyang Chu, and Tszer Hong Lin
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Drift velocity ,Meteorology ,Terminal velocity ,Condensed Matter Physics ,Wind profiler ,Wind speed ,law.invention ,Atmosphere ,Drag ,law ,Physics::Space Physics ,General Earth and Planetary Sciences ,Environmental science ,Precipitation ,Electrical and Electronic Engineering ,Radar ,Physics::Atmospheric and Oceanic Physics - Abstract
One of the essential assumptions made in estimating the terminal velocity and the drop-size distribution of hydrometeor from the precipitation Doppler spectrum observed by VHF/UHF radar (or wind profiler) is that all of the precipitation particles are moving together at the same velocity as the ambient wind. However, this may not be the case. In this paper, taking advantage of the capability of simultaneously measuring the motions of precipitation particles and the clear-air turbulence with a VHF radar, we develop a method to examine the wind-driven effect on the drift of precipitation particles using the Chung-Li VHF radar. By appropriately setting the radar parameters and steering the radar beams, experimental results show that the drift velocity of the precipitation particles in response to the drag force exerted by the background atmosphere is not the same as the ambient wind velocity, approximately 83% of the latter in this case. This feature indicates that the precipitation particles are not moving with the background wind, causing inaccurate estimations of the terminal velocity and the drop-size distribution of hydrometeor if this effect is not taken into account.
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- 1997
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7. Adamtsl3 mediates DCC signaling to selectively promote GABAergic synapse function.
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Cramer, Teresa M.L., Pinan-Lucarre, Berangere, Cavaccini, Anna, Damilou, Angeliki, Tsai, Yuan-Chen, Bhat, Musadiq A., Panzanelli, Patrizia, Rama, Nicolas, Mehlen, Patrick, Benke, Dietmar, Karayannis, Theofanis, Bessereau, Jean-Louis, and Tyagarajan, Shiva K.
- Abstract
The molecular code that controls synapse formation and maintenance in vivo has remained quite sparse. Here, we identify that the secreted protein Adamtsl3 functions as critical hippocampal synapse organizer acting through the transmembrane receptor DCC (deleted in colorectal cancer). Traditionally, DCC function has been associated with glutamatergic synaptogenesis and plasticity in response to Netrin-1 signaling. We demonstrate that early post-natal deletion of Adamtsl3 in neurons impairs DCC protein expression, causing reduced density of both glutamatergic and GABAergic synapses. Adult deletion of Adamtsl3 in either GABAergic or glutamatergic neurons does not interfere with DCC-Netrin-1 function at glutamatergic synapses but controls DCC signaling at GABAergic synapses. The Adamtsl3-DCC signaling unit is further essential for activity-dependent adaptations at GABAergic synapses, involving DCC phosphorylation and Src kinase activation. These findings might be particularly relevant for schizophrenia because genetic variants in Adamtsl3 and DCC have been independently linked with schizophrenia in patients. [Display omitted] • Adamtsl3 is widely expressed at hippocampal synapses • Adamtsl3 is a ligand for DCC during synaptogenesis and in the mature brain • Presynaptic and postsynaptic Adamtsl3 contribute toward GABAergic synapse maintenance via DCC • Autocrine Adamtsl3-DCC signaling mediates plasticity adaptations at GABAergic postsynapse The secreted glycoprotein Ce-Punctin clusters neurotransmitter receptors at the C. elegans NMJ. Cramer et al. reveal that Ce-Punctin ortholog Adamtsl3 is a synaptic protein influencing synapse formation via the DCC receptor in the mouse brain. In the adult hippocampus, Adamtsl3-DCC signaling specializes toward GABAergic synapse function with behavioral consequences. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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8. RhoGEF9 splice isoforms influence neuronal maturation and synapse formation downstream of α2 GABAA receptors.
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de Groot, Claire, Floriou-Servou, Amalia, Tsai, Yuan-Chen, Früh, Simon, Kohler, Manuela, Parkin, Georgia, Schwerdel, Cornelia, Bosshard, Giovanna, Kaila, Kai, Fritschy, Jean-Marc, and Tyagarajan, Shiva K.
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NEURAL circuitry ,SYNAPSES ,GABA receptors ,SPLICEOSOMES ,ACTIN ,DEVELOPMENTAL neurobiology - Abstract
In developing brain neuronal migration, dendrite outgrowth and dendritic spine outgrowth are controlled by Cdc42, a small GTPase of the Rho family, and its activators. Cdc42 function in promoting actin polymerization is crucial for glutamatergic synapse regulation. Here, we focus on GABAergic synapse-specific activator of Cdc42, collybistin (CB) and examine functional differences between its splice isoforms CB1 and CB2. We report that CB1 and CB2 differentially regulate GABAergic synapse formation in vitro along proximal-distal axis and adult-born neuron maturation in vivo. The functional specialization between CB1 and CB2 isoforms arises from their differential protein half-life, in turn regulated by ubiquitin conjugation of the unique CB1 C-terminus. We report that CB1 and CB2 negatively regulate Cdc42; however, Cdc42 activation is dependent on CB interaction with gephyrin. During hippocampal adult neurogenesis CB1 regulates neuronal migration, while CB2 is essential for dendrite outgrowth. Finally, using mice lacking Gabra2 subunit, we show that CB1 function is downstream of GABA
A Rs, and we can rescue adult neurogenesis deficit observed in Gabra2 KO. Overall, our results uncover previously unexpected role for CB isoforms downstream of α2-containing GABAA Rs during neuron maturation in a Cdc42 dependent mechanism. [ABSTRACT FROM AUTHOR]- Published
- 2017
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9. The behavioural response of mice lacking NK1 receptors to guanfacine resembles its clinical profile in treatment of ADHD.
- Author
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Pillidge, Katharine, Porter, Ashley J, Dudley, Julia A, Tsai, Yuan-Chen, Heal, David J, and Stanford, S Clare
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TREATMENT of attention-deficit hyperactivity disorder ,GUANFACINE ,SUBSTANCE P receptors ,LABORATORY mice ,MICE behavior ,ABLATION techniques ,THERAPEUTICS - Abstract
Background and Purpose Mice with functional ablation of substance P-preferring neurokinin-1 receptors ( NK1R−/− mice) display behavioural abnormalities resembling those in attention deficit hyperactivity disorder ( ADHD). Here, we investigated whether the ADHD treatment, guanfacine, alleviated the hyperactivity and impulsivity/inattention displayed by NK1R−/− mice in the light/dark exploration box ( LDEB) and 5-choice serial reaction-time task (5- CSRTT), respectively. Following reports of co-morbid anxiety in ADHD, we also investigated effects of guanfacine on anxiety-like behaviour displayed by NK1R−/− and wild-type (WT) mice in the elevated plus maze ( EPM). Experimental Approach Mice were treated with guanfacine (0.1, 0.3 or 1.0 mg·kg
−1 , i.p.), vehicle or no injection and tested in the 5- CSRTT or the LDEB. Only the lowest dose of guanfacine was used in the EPM assays. Key Results In the 5- CSRTT, a low dose of guanfacine (0.1 mg·kg−1 ) increased attention in NK1R−/− mice, but not in WT mice. This dose did not affect the total number of trials completed, latencies to respond or locomotor activity in the LDEB. Impulsivity was decreased by the high dose (1.0 mg·kg−1 ) of guanfacine, but this was evident in both genotypes and is likely to be secondary to a generalized blunting of behaviour. Although the NK1R−/− mice displayed marked anxiety-like behaviour, guanfacine did not affect the behaviour of either genotype in the EPM. Conclusions and Implications This evidence that guanfacine improves attention at a dose that did not affect arousal or emotionality supports our proposal that NK1R−/− mice express an attention deficit resembling that of ADHD patients. Linked Articles This article is part of a themed section on Animal Models in Psychiatry Research. To view the other articles in this section visit [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
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