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Background: Near-infrared photoimmunotherapy (NIR-PIT) is an emerging cancer treatment technology that combines the advantages of optical technology and immunotherapy to provide a highly effective, precise, and low side-effect treatment approach. The aim of this study is to visualize the scientific results and research trends of NIR-PIT based on bibliometric analysis methods. Methods: The Web of Science Core Collection (WoSCC) database was searched in August 2024 for relevant publications in the field of NIR-PIT. Data were analyzed using mainly CiteSpace and R software for bibliometric and visual analysis of the country/region, authors, journals, references and keywords of the publications in the field. Results: A total of 245 publications were retrieved, including articles (n = 173, 70.61%) and reviews (n = 72, 29.39%). The annual and cumulative number of publications increased every year. The highest number of publications was from the United States (149, 60.82%), followed by Japan (70, 28.57%) and China (33, 13.47%). The research institution with the highest number of publications was National Institutes of Health (NIH)-USA (114, 46.53%). Kobayashi H (109) was involved in the highest number of publications, Mitsunaga M (211) was the most frequently cited in total. CANCERS (17) was the most frequently published journal, and NAT MED (220) was the most frequently co-cited journal. The top 10 keywords include near-infrared photoimmunotherapy (166), photodynamic therapy (61), monoclonal antibody (58), in vivo (50), cancer (46), expression (31), breast cancer (27), enhanced permeability (24), antibody (23), growth factor receptor (16). Cluster analysis based on the co-occurrence of keywords resulted in 13 clusters, which identified the current research hotspots and future trends of NIR-PIT in cancer treatment. Conclusion: This study systematically investigated the research hotspots and development trends of NIR-PIT in cancer treatment through bibliometric and visual analysis. As an emerging strategy, the research on the application of NIR-PIT in cancer treatment has significantly increased in recent years, mainly focusing on the targeting, immune activation mechanism, and treatment efficacy in solid tumors has received extensive attention. Future studies may focus on improving the efficacy and safety of NIR-PIT in cancer treatment, as well as developing novel photosensitizers and combination therapeutic regimens, and exploring the efficacy of its application in a wide range of solid tumors, which will provide an important reference and guidance for the application of NIR-PIT in clinical translation. [ABSTRACT FROM AUTHOR]

The article in Clinical & Translational Medicine delves into the comprehensive profiling of circular RNA in colorectal cancer (CRC) to understand its pathogenesis and identify potential diagnostic biomarkers. By analyzing circRNA alterations in CRC tissues, the study reveals distinct tissue-specific differences and identifies circRNAs that effectively distinguish tumor tissues. The study also constructs a circRNA-miRNA-mRNA regulatory network and explores the functional roles of differentially expressed circRNAs, suggesting their potential as staging biomarkers and early diagnostic tools for CRC. The research emphasizes the clinical relevance of circRNAs in CRC development and provides valuable insights into their diagnostic and therapeutic implications. [Extracted from the article]

Subitizing is the ability to appraise a number of small quantities (up to four) rapidly and precisely. This system, however, can be impaired by distractors presented along with targets to be enumerated. To better understand whether this limitation arises in perceptual circuits or in the response selection stage, we investigated whether subitizing can endure in simultaneous comparison tasks. Participants were asked to compare the number of dots in two sets on the left and right sides of the screen, presented either simultaneously or sequentially. For comparing within the numerosity range (6–32 dots), both the error rate and reaction time increased steadily as the ratio between the two numbers compared approached "1." Namely, a phenomenon labeled the ratio effect was revealed. For comparison with small numbers (<5), the sequential comparison task was errorless despite the ratio, suggesting the feature of subitizing. Individual efficiency (measured by the inverse efficiency score [IES]) did not correlate between number ranges in sequential comparison, suggesting that distinct mechanisms were involved. However, we found that in simultaneous tasks, error rate and efficiency showed an increase as the ratios of the two numbers compared approached "1." This is similar to the ratio effect revealed in the comparison for moderate numbers. Individual efficiency within these two ranges correlated, indicating that the enumeration within these two ranges was based on a single mechanism. These results suggest that subitizing cannot process sets in parallel, and numerosity takes the job whenever subitizing fails. [ABSTRACT FROM AUTHOR]

Background: Pulmonary hypertension (PH) is a progressive disease characterized by pulmonary vascular remodeling. Increasing evidence indicates that endothelial-to-mesenchymal transition (EndMT) in pulmonary artery endothelial cells (PAECs) is a pivotal trigger initiating this remodeling. However, the regulatory mechanisms underlying EndMT in PH are still not fully understood. Methods: Cytokine-induced hPAECs were assessed using RNA methylation quantification, qRT-PCR, and western blotting to determine the involvement of N6-methyladenosine (m6A) methylation in EndMT. Lentivirus-mediated silencing, overexpression, tube formation, and wound healing assays were utilized to investigate the function of METTL3 in EndMT. Endothelial-specific gene knockout, hemodynamic measurement, and immunostaining were performed to explore the roles of METTL3 in pulmonary vascular remodeling and PH. RNA-seq, RNA Immunoprecipitation-based qPCR, mRNA stability assay, m6A mutation, and dual-luciferase assays were employed to elucidate the mechanisms of RNA methylation in EndMT. Results: The global levels of m6A and METTL3 expression were found to decrease in TNF-α- and TGF-β1-induced EndMT in human PAECs (hPAECs). METTL3 inhibition led to reduced endothelial markers (CD31 and VE-cadherin) and increased mesenchymal markers (SM22 and N-cadherin) as well as EndMT-related transcription factors (Snail, Zeb1, Zeb2, and Slug). The endothelial-specific knockout of Mettl3 promoted EndMT and exacerbated pulmonary vascular remodeling and hypoxia-induced PH (HPH) in mice. Mechanistically, METTL3-mediated m6A modification of kruppel-like factor 2 (KLF2) plays a crucial role in the EndMT process. KLF2 overexpression increased CD31 and VE-cadherin levels while decreasing SM22, N-cadherin, and EndMT-related transcription factors, thereby mitigating EndMT in PH. Mutations in the m6A site of KLF2 mRNA compromise KLF2 expression, subsequently diminishing its protective effect against EndMT. Furthermore, KLF2 modulates SM22 expression through direct binding to its promoter. Conclusions: Our findings unveil a novel METTL3/KLF2 pathway critical for protecting hPAECs against EndMT, highlighting a promising avenue for therapeutic investigation in PH. [ABSTRACT FROM AUTHOR]

The yellow seal stone from northern Laos is one possible substitute for the Tianhuang Stone, the most famous Chinese seal stone, because of its similar yellow to orange-yellow appearance and the same main mineral composition. The colour causation of the yellow seal stone from northern Laos was studied. The samples' phase, micro-morphology and chemical components were studied by Raman spectroscopy, and scanning electron microscopy (SEM) with energy disperse spectroscopy (EDS), respectively. The yellow seal stone from northern Laos is mainly composed of dickite, occasionally with minor impurity minerals, such as hematite, anatase, barite, diaspore and pyrite. Micro- to nano-sized iron oxides/hydroxides were observed and detected by SEM and EDS in the yellow to orange-yellow part of the samples. Moreover, these iron oxides/hydroxides were suggested to cause the yellow to orange-yellow in the seal stone from northern Laos. The UV-Vis spectrum and its second derivative, the Kubelka-Munk spectra, were used to identify and quantify hematite and goethite. The samples' colour parameters were obtained with the Commission Internationale de l'Eclairage (CIE) 1931 standard space. According to the observation of the samples and the results obtained from experiments and calculations, the colour of the yellow parts (L* = 33.56~47.99, a* = 0.35~3.65, b* = 4.55~9.89) correlated with goethite (goethite is about 0.175~0.671 g/kg, the content of hematite was too low to be figured out in the yellow parts). In contrast, the colour of the orange-yellow parts (L* = 33.99~46.27, a* = 3.98~12.39, b* = 8.04~22.14) was more closely related with the content of hematite (goethite is about 0.096~0.691 g/kg, hematite is about 0.258~2.383 g/kg). The results of correlation analysis also support that the contents of iron oxides or hydroxides influence the samples' colour. Therefore, it is suggested that micro- to nano-scaled hematite and goethite caused the colour of yellow and orange-yellow in the studied seal stone. Hematite can strengthen the red hue and change the colour from yellow to orange-yellow. [ABSTRACT FROM AUTHOR]

Background: Ovarian cancer (OC) is the most lethal gynecological malignancy worldwide. Increasing evidence indicates that TBC domain family is implicated in various cellular events contributing to initiation and development of different cancers, including OC. However, the role of TBC1D2, a crucial member of TBC domain family, remains unclear in OC. Methods: IHC and qRT-PCR were employed to determine TBC1D2 expression in OC tissues and cells. In vitro and in vivo assays involving proliferation, migration, invasion were performed to explore the role of TBC1D2 in OC development. The underlying mechanism by which TBC1D2 promotes OC metastasis were elucidated using bioinformatics analysis, western blotting and co-immunoprecipitation. Results: Upregulation of TBC1D2 was found in OC and was associated with a poor prognosis. Meanwhile, TBC1D2 promoted OC cell proliferation, migration, and invasion in vitro and facilitated tumor growth and metastasis in vivo. Moreover, TBC1D2 contributed to OC cell invasion by E-cadherin degradation via disassembling Rac1-IQGAP1 complex. In addition, miR-373-3p was screened out and identified to inhibit OVCAR3 invasion via negative regulation of TBC1D2. Conclusion: Our findings indicated that TBC1D2 is overexpressed in OC and contributes to tumor metastasis via E-cadherin degradation. This study suggests that TBC1D2 may be an underlying therapeutic target for OC. [ABSTRACT FROM AUTHOR]

Since CdSe quantum dots (QDs) are increasingly used in electronics, medical, and pharmaceutical science due to their excellent optical properties, it is necessary to carry out thorough and systematic studies on their biosafety. Numerous studies have reported the toxicity of QDs on liver, kidney, immune system, and reproductive system. However, few studies have been done on the cardiotoxicity of QDs. In this study, we administered carboxylated CdSe/ZnS QDs in BALB/c mice via the tail vein and analyzed the in vivo cardiotoxicity of CdSe/ZnS QDs. The body weight, hematology, serum biochemistry, histology, heart elements concentration, echocardiography, and heart oxidative stress markers were carried out at different time. There were no significant differences in body weight and heart organ index between QDs group and the control group. Hematology results showed the platelet (PLT) counts on Day 1 and Day 42 in both high dose QDs group and low dose QDs group, and the PLT counts on Day1 in the high dose group were significantly higher than that in control group. Serum biochemistry results showed that lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase isoenzyme (CK-MB) of mice exposed to CdSe/ZnS QDs were significantly higher than that of the control group on Day 1, and CK-MB levels still remained high on Day 7. A higher concentration of Cd was observed in the heart of CdSe/ZnS QDs exposed mice on Day 42, whereas no Cd was detected in the control group, which suggested that QDs can accumulate in heart. No significant histopathological changes and cardiac function were observed in all mice at different time after treatment. Increased level of glutathione peroxidase (GPx) and malondialdehyde (MDA) was observed in mice administered with high dose QDs on Day 1, and increased level of total antioxidant capacity (T-AOC) and MDA activities was observed on Day 42. These results indicated that CdSe/ZnS QDs could accumulate in heart, cause some biochemical indicators change, induce oxidative damage, and have cardiotoxicity. Our findings might provide valuable information on the biological safety evaluation of the cardiovascular system of QDs. [ABSTRACT FROM AUTHOR]

The clinical application of RNA interference (RNAi)-based cancer gene therapy has been hampered by the lack of efficient delivery of short interfering RNA (siRNA). In this context, the use of biodegradable charged polyester-based vectors (BCPVs) for delivering mutated K-Ras-targeting siRNA in a pancreatic xenograft model was investigated in vivo. Using mice bearing pancreatic xenografts as an animal model, results show that fluorescently labeled TRAMA (carboxytetramethylrhodamine) K-Ras siRNA continuously accumulated in the xenograft via BCPVs for at least 72 h. After the treatment, the level of the targeted mRNA and protein reduced to 50% of their original level. As a consequence, significant suppression in tumor growth, decreased tumor local infiltration, and increased cell apoptosis were observed in the xenograft model after the siRNA treatment. More importantly, physiological analysis results reveal that an excessive amount of BCPV (10 times higher than the commonly treated amount) will not have a significant influence on the status of the blood stream, blood stream components, and organ tissue, suggesting that BCPVs have very low in vivo toxicity. Our results indicate that the delivery of K-Ras-targeting siRNA via BCPV nanoparticles may be a promising strategy for pancreatic cancer therapy. [ABSTRACT FROM AUTHOR]

Zinc sulfide (ZnS) has been widely studied due to its versatile application in photocatalysis. A facile, one-pot, ultrasonic-assisted approach was developed to synthesize coaxial nanocables consisting of highly conductive multi-walled carbon nanotube (CNT) cores and well-crystalline ZnS sheaths. The morphology and structural features of the materials were characterized using scanning electron microscopy, transmission electron microscopy, and X-ray diffraction analysis. The photoluminescence and optical properties of the CNT/ZnS coaxial nanocables are characterized by photoluminescent spectrum and UV-Vis spectroscopy. Methyl orange (MO) dye was chosen as a model for organic pollutants and was used to evaluate the photocatalytic performance of the CNT/ZnS nanocables. CNT/ZnS showed significant visible-light activity in the degradation of MO. Compared with the ZnS powders, the novel CNT/ZnS coaxial nanocables exhibited much higher performance as a photocatalyst. The enhanced performance arises from the structural advantages of the nanocables. First, the CNT cores effectively improved the electronic conductivity of the hybrid materials. Second, the CNT coaxial nanocables had rich hierarchical pores of ZnS sheath and large surface area. [ABSTRACT FROM AUTHOR]

Easily reproducible magnetic carbon nanoadsorbents (MPSNs) derived from polystyrene (PS) by direct calcination of functionalized PS with an iron salt are employed for effective tetrabromobisphenol A (TBBPA) adsorption. Batch adsorption tests indicate that MPSNs exhibited high adsorption affinity to aqueous TBBPA with a maximum adsorption capacity of 117.00 mg g−1. The TBBPA adsorption kinetics of MPSNs are found to obey the pseudo-second-order behavior with a calculated room temperature initial adsorption rate of 42.871 mg g−1 min−1 for the solution with an initial TBBPA concentration of 4.0 mg L−1 and pH value of 8.0. Monolayer adsorption of the Langmuir isotherm model is well fitted rather than the multilayer adsorption of the Freundlich isotherm model. The calculated thermodynamic parameters suggest that the TBBPA adsorption on MPSNs is spontaneous and exothermic. The optimal pH value for TBBPA adsorption on MPSNs is around 8.0 with an MPSNs dose of 30.0 mg L−1 and contact time of 30 min under sonication at 298 K. The TBBPA adsorption performance of MPSNs is strongly influenced by the presence of humic acid. The prepared MPSN retains around 85.5% of TBBPA adsorption capacity after 5 cycles and exhibits good reusability. The explored adsorption mechanism by Raman and Fourier transform infrared (FT-IR) spectroscopy suggests that the C–O–Fe bond in the MPSN is responsible for the superior TBBPA adsorption performance. This work provides promising magnetic adsorbents for TBBPA wastewater treatment as well as a new strategy to recycle and reuse polymer plastic waste. [ABSTRACT FROM AUTHOR]

In this work, the combined effects of graphene oxide (GO) and Cd 2+ solution on Microcystis aeruginosa were investigated. Chlorophyll fluorescence parameters were measured by a pulse-amplitude modulated fluorometer. GO at low concentrations exhibited no significant toxicity. The presence of GO at low concentrations significantly enhanced Cd 2 + toxicity as the 96 h half maximal effective concentration of the Cd 2+ reduced from 0.51 ± 0.01 to 0.474 ± 0.01 mg/L. However, concentrations of GO above 5 mg/L did not significantly increase the toxicity of the Cd 2+ /GO system. Observations through scanning and transmission electron microscopy revealed that GO, with Cd 2+ , easily attached to and entered into the algae. Reactive oxygen species and malondialdehyde were measured to explain the toxicity mechanism. The photosynthetic parameters were useful in measuring the combined toxicity of the nanoparticles and heavy metals. [ABSTRACT FROM AUTHOR]

In this work, we report the synthesis of dye-loaded and folic acid (FA)-conjugated organically modified silica (ORMOSIL) nanoparticles as targeted optical nanoprobes for in vitro and in vivo imaging. The dye-loaded ORMOSIL (ORMD) nanoparticles are synthesized by a facile aqueous phase (oil-in-water microemulsion) approach and they have an average size of 30 nm. We observed that the functionalization of FA onto the particle surface led to a strong cellular uptake of FA-conjugated ORMD nanoparticles for pancreatic cancer Miapaca-2 cells and hepatoma SMMC7721 cells with FA receptor overexpression. Such a trend is not observed for 293T cells and breast cancer MCF7 cells as these cells possess low-expression of the FA receptor. The in vivo imaging studies demonstrate that FA-ORMD nanoparticles are preferentially accumulated in tumor sites. Histological studies reveal that no-ill effects are observed in the major organs of treated mice when compared to the untreated ones. Because of the facile synthesis process, high specificity for tumor targeting and low toxicity of FA-ORMD nanoparticles, significant potential for early-cancer detection application is expected. [ABSTRACT FROM AUTHOR]

Perfluorooctanoic acid (PFOA) is one of the most commonly used perfluorinated chemicals in industry. Wide concerns of PFOA toxicity are increased in recent years. However, report on immunotoxicity of PFOA was quite limited. This study aimed to investigate the immunotoxicity of PFOA exposure on macrophage RAW264.7. We assessed the effects of PFOA exposure on macrophage cell viability, cell apoptosis and cellular ROS level, and detected prominent cytokines release by RAW264.7. The results indicated that the cell viability of macrophage RAW264.7 was decreased by PFOA in dose- and time-dependent manners. Specifically, the exposure of 200 μM PFOA significantly increased apoptosis and ROS generation in macrophage, and thus caused cell damage. The ELISA results displayed that 100 μM PFOA exposure induced macrophage activation and enhanced cytokines secretion, including TNF-α, IL-1, IL-6, and IL-12. We also conducted nontargeted metabolomics based on LC-MS/MS and unveiled the perturbed metabolic pathways in macrophages induced by sublethal doses of PFOA (10 μM and 100 μM). Remarkably, global metabolomics results displayed that 10 μM PFOA exposure affected glutamine related pathways and the exposure at 100 μM conspicuously changed glutathione and fatty acid oxidation metabolism. These findings showed that 10 μM PFOA exposure could impel metabolic reprogramming of macrophage to trigger inflammatory response, although such dose displayed no obvious effect on cell viability, cellular ROS or apoptosis events of macrophage RAW264.7. • Cytotoxicity assessment of PFOA on macrophage. • Metabolomics study of PFOA effect on macrophage. • Cytokines analysis of macrophage exposed to PFOA. • Elaborate pipeline and methods for macrophage metabolomics analysis. • PFOA impels metabolic reprogramming of macrophage that activates immune response. [ABSTRACT FROM AUTHOR]

Cadmium has been widely detected in the environment and various foods. The association between cadmium burden and osteoporosis has been studied in cohorts. However, the effects and mechanisms of environmental cadmium exposure on bone metabolism is poorly understood. This study aims to investigate the altered metabolites in bone cells affected by low-level cadmium by metabolomics analysis. Specifically, we used the dosage of cadmium that do not decrease the cell viability (determined by MTT assay) to treat Saos-2 cells for 24 h. ICP-MS was applied to quantify the cadmium in culture medium and cell precipitate. The cellular metabolites were extracted and analyzed by liquid chromatography-mass spectrometry. The pathway analysis based on the identified differential metabolites showed that 1 μM cadmium significantly affected citric acid cycle and malate-aspartate shuttle, while 10 μM cadmium treatment affected citric acid cycle, alanine metabolism, glucose-alanine cycle, pyrimidine metabolism and glutamate metabolism. Taken together, 1 μM cadmium exposure could suppress the electrons transportation from the cytosol to mitochondrial matrix in Saos-2, and the impediment of the electron transport chain further inhibited downstream activities in citric acid cycle, which resulted in the accumulation of pyruvic acid. In addition, the suppressed pyrimidine degradation resulted in senescent nucleic acid accumulation and the decrease of mRNA transcription in Saos-2 cells. In general, our studies unveil the cadmium-induced metabolic perturbations in Saos-2 cells and demonstrate the feasibility of our established metabolomics pipeline to understand cadmium-induced effects on bone. Image 1 • Metabolomics study of cadmium effect on human Saos-2 bone cell line. • Elaborate pipeline and methods for cell metabolomics analysis. • Dietary dosage-related cadmium exposure induced mitochondria dysfunction. • Cadmium perturbed the metabolism of amino acid and nucleic acids. [ABSTRACT FROM AUTHOR]

Tetrodotoxin (TTX) is a potent neurotoxin isolated mainly from toxic puffer fish. To date, the TTX biosynthetic mechanism inside its hosts remains unresolved. Here, we hypothesize the TTX synthesis relies on the host gut microbiota, including the neglected non-culturable bacteria. In these studies, we collected the gut contents from 5 puffer fish species of the genus Takifugu including one suspected hybrid species for gut microbiota study by 16S rRNA amplicon metagenomics approach. Their gut samples were divided into toxic and non-toxic groups based on the TTX concentrations in the livers detected by LC-MS/MS. Bacterial diversity studies showed that gut microbiota structures were significantly different between toxic and non-toxic species. Vibrio and Cyanobacteria centered at the gut bacterial co-occurrence network, suggesting their importance in TTX biosynthesis. The results of PICRUSt2 metagenomic prediction and gene set enrichment analysis provided new support of arginine-precursor required in TTX biosynthesis. This is the first study to profile the gut microbiota in toxic and non-toxic puffer fish species by 16S rRNA amplicon metagenomic approach, defining significant microbial co-occurrence patterns in their gut environment. Our data supported the proposed biosynthesis of TTX inside the hosts by their gut bacterial symbionts using arginine as a precursor. [ABSTRACT FROM AUTHOR]

[Display omitted] • Urines from female and male residents under cadmium exposure were investigated by mass spectrometry-based metabolomics. • Biomarkers and metabolic pathways related to cadmium-induced nephrotoxicity were characterized. • This study might provide information to reflect the body burden and help improve health policy for risk assessment. Cadmium is a well-known hazardous pollutant that mainly comes from dietary, tobacco and occupational exposure, posing threat to kidney. However, there is still a lack of systematic study on metabolic pathways and urinary biomarkers related to its nephrotoxicity under cadmium exposure for both females and males. In this study, a mass spectrometry-based metabolomics investigation of a cohort of 144 volunteers was conducted to explore sex-specific metabolic alteration and to screen biomarkers related to cadmium-induced nephrotoxicity. When the concentration of urinary cadmium increased, creatine pathway, amino acid metabolism especially the tryptophan metabolism, aminoacyl-tRNA biosynthesis, and purine metabolism were primarily influenced regardless of the gender. Also, the most specific biomarkers linked with nephrotoxicity based on the statistical analysis were detected including creatine, creatinine, l -tryptophan, adenine and uric acid. The study outcome might provide information to reflect the body burden and help improve health policy for risk assessment. [ABSTRACT FROM AUTHOR]

• Two different indica rice grains under Cd stress were subjected to metabolomics analysis. • Characteristic responses for carbohydrate, organic acid and amino acid metabolic pathways. • This study may aid selection of rice varieties with low cadmium accumulation. Cadmium is a toxic environmental pollutant that is readily absorbed by rice grains and poses serious threats to human health. The selection and breeding of rice varieties with low cadmium accumulation is one of the most economical and ecological methods to reduce cadmium exposure. In this study, two different indica rice grains under cadmium stress were subjected to mass spectrometry-based metabolomics analysis for the first time. When the cadmium concentration increased in rice grains, most carbohydrates and amino acids were down-regulated, except myoinositol that can prevent cadmium toxicity, which was up-regulated. d -Mannitol and l -cysteine were up-regulated with the increase of cadmium concentration in low-cadmium-accumulating rice. Also, organic acids were activated especially 13-(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid that is related to the alpha-linolenic acid metabolism and jasmonic acid production. The determination of biomarkers and characterization of metabolic pathways might be helpful for the selection of rice varieties with low cadmium accumulation. [ABSTRACT FROM AUTHOR]