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Passive design strategies enhance indoor thermal comfort and reduce energy consumption in buildings located in hot climates. Thus, exploring the various zones of a house's yard can yield valuable insights for devising a plan that achieves high energy and lighting efficiency. This study examines the climate-responsive design strategies of 20 traditional courtyard buildings in Birjand's hot and dry climate. These courtyard houses informed modelling a new hypothetical house in DesignBuilder. Two main orientations (45° = NE–SW, and 315° = NW–SE) were used for comparing their impact on indoor air temperature, solar gain and daylight factor across various courtyard zones. The findings indicate that during winter, the interiors of courtyard houses maintain a temperature higher than the exterior, while in summer, they remain cooler than outside conditions. Concerning the influence of orientation on indoor climates, the NE–SW orientation yielded the greatest solar gains for windows in the northern and western zones. Conversely, in the NW–SE orientation, the most substantial solar gains were recorded for windows in the northern and eastern zones. Additionally, the study noted a marginal variation in the daylight factor across different zones of the courtyard houses when comparing the two orientations. [ABSTRACT FROM AUTHOR]

Familial adenomatous polyposis (FAP) is an autosomal dominant colorectal cancer syndrome that is characterized by the development of multiple adenomas in the colon and rectum with high penetrance rates. This disease has specific features like the occurrence of pathogenic variations in the APC gene and diverse FAP phenotypes due to the occurrence region. In this study we aimed to evaluate pathogenic variants in exons of the APC gene in Iranian patients with FAP. A total of 35 FAP individuals were referred to the gastroenterology ward of Taleghani Hospital. As the aim of the study was to study the germline variations in the participants, the peripheral blood was collected and after the DNA extraction, PCR, and Sanger sequencing processes for the APC gene, the results were evaluated by the ACMG classification guidelines to report their pathogenicity. Accordingly, out of eight specific detected variants, three of them were novel, and the rest were reported previously. These eight variants were all truncating protein and pathogenic, and they were limited to 849-1378 codons. Overall, detected variants revealed discrepancies and parallels with previous reported cases in terms of quantity, occurrence region, and association with demographic and clinicopathological characteristics of patients. The spectrum of detected variants and the patient's phenotype showed distinct characteristics, such as occurrence in specific regions and the absence of extracolonic symptoms like Congenital hypertrophy of the retinal pigment epithelium (CHRPE). These findings open the path to comprehending the typical symptoms, their rarity, and their occurrence in the Iranian population and also due to the facts, we found that the studying of the APC gene alone for diagnosing FAP disease is not sufficient, and considering other genes are completely rational in the case of sequencing and studying the variants. [ABSTRACT FROM AUTHOR]

Based on the analysis of patients with Peutz-Jeghers syndrome (PJS), Serine threonine kinase11 (STK11) is known as a tumor suppressor gene, which is involved in cell polarization, regulation of apoptosis, and DNA damage response. In this case report study, we examined STK11 gene sequencing in a 42-year-old woman with mucocuta neous pigmentation and positive family history. Endoscopy and colonoscopy showed >1000 polyps throughout the stomach/colon (PJ-type hamartomas). The larger polyp in the stomach was resected and the small bowel imaging detected multiple jejunum/ileum small polyps. The data released from the sequencing results revealed five alterations in exons 1 to 5. The major mutation in stop codon was reported as converted to the amino acid tryptophan (TRP) to tyrosine (TER). The TGG codon was converted to TAG by mutation. Finally, another novel mutation in STK11 stop codon as a 'de novo' variant was seen. It is predicted that stop codon mutations make the affected person susceptible to developing colorectal cancer. [ABSTRACT FROM AUTHOR]

The energy released during the bubble collapse process is used for medical and industrial purposes. The present study investigates the effects of electrohydrodynamic force on the collapse phenomenon near the rigid wall and the enhancement of the collapse energy. A solver in the OpenFoam open-source code is developed based on the volume-of-fluid model, in which the effects of compressibility, energy transfer, and electrohydrodynamic force are included. The developed solver is validated against the available experimental data, and a good agreement is seen. The effects of an electric field on the bubble collapse for the range of the electrocapillary number (CaE) of 0–5.76 and normalized wall distance (γ) of 0.8–2.0 are investigated. The results indicate that the bubble is deformed due to the presence of an electric field, and the values obtained for the maximum velocity and pressure are 33 and 35 times the state without the electric field at γ = 2 and CaE = 5.76, respectively. Also, due to the increase in velocity, the maximum shear stress on the rigid wall is increased up to seven times in the absence of the electric field. Therefore, the jet force obtained from the bubble collapse can be enhanced by applying the electric field in the continuous phase fluid. Also, the correlations are proposed to estimate the jet velocity, pressure, and wall shear stress of bubble collapse in the presence of an electric field. [ABSTRACT FROM AUTHOR]

Thermal comfort in cities is an influential factor for citizens' wellbeing and life quality. Urban microclimate studies have gained popularity following increasing urbanization trends and global climate change in recent years. Urban fabric and morphology in traditional cities represent a unique pattern both spatially and climatically. However, few studies have investigated traditional cities' urban thermal comfort conditions. Therefore, this study aimed to assess the thermal comfort in different subspaces of Algiers Casbah's historic urban fabric, which falls in the hot Mediterranean climate (Csa). This research evaluated the human thermal sensation by applying the physiological equivalent temperature (PET) index. The methodology used was a mixed approach, including field measurements, calculations, and a survey questionnaire. The results indicate the presence of a high-stress level during the measurement periods, and notable differences between the subspaces in January (ΔPETMax.Jan = 3.7 °C) and August (ΔPETMax.Aug = 2.2 °C). The highest discomfort was recorded in spaces with collapsed buildings, especially during the hot hours of the day. The findings also highlight a strong impact of the sky view factor on the mean radiant temperature (Tmrt) and the physiological equivalent temperature (PET). The study discusses recommendations and ways to improve the design of outdoor spaces and relieve heat stress in the streets of traditional cities. Finally, this work helps urban managers and heritage conservators in urban rehabilitation policies concerning outdoor microclimate improvement. [ABSTRACT FROM AUTHOR]

Impacts of the laminar airflow ventilation system design factors on contaminant removal and thermal comfort condition in an operating room have been investigated by means of Lagrangian-based particle transport using the non-Boussinesq modeling of the buoyancy effects. An operating room including staffs and a patient with realistic human geometries and two surgery lights are included in simulations. The laminar airflow system is placed on the ceiling with a surrounding fixed-height partial wall and the air barrier supply grills. Effects of density change in mixed convection flow regime are included by the non-Boussinesq modeling of the exact air density variation. The predicted mean vote, the age of air, the colony-forming units per cubic meter, the average temperature, the average velocity, the relative humidity, the density distribution, and the positions of particles are calculated to assess the indoor air ventilation quality. A total of 27 simulation cases have been considered to determine the impact of three main design factors including the laminar airflow system area, the supply air, and the air barrier velocities on the performance of the system. It is concluded that for the curtain velocity of 2 m/s, the thermal comfort reduces with increasing the laminar airflow velocity, but for the third staff, the results show that for small laminar airflow areas, the speed of the inlet port should be reduced and for the larger sizes, the inverse of this trend is recommended. Moreover, for all cases the humidity varies within the range of 55%–56%, which agrees well with the suggested standard humidity range between 50% and 60%. It is concluded that the cases of the laminar airflow velocity equal to 0.3 and 0.5 at the curtain velocity of 1 m/s are generally more appropriate than other cases due to less accommodation of particles near the entire body of the patient. [ABSTRACT FROM AUTHOR]

Aim: This study aimed to evaluate the distribution of PIK3CA E545K mutation in Iranian CRC patients and explored its roles in disease prognosis. Background: Deregulation of the phosphoinositide 3-kinase (PI3K) pathway contributes to the progression of tumors. The p110a (PIK3CA), a catalytic subunit of PIK3, is mutated in many types of cancers. Exon 9 (E545K) is the most frequently mutated hotspot in PIK3CA in colorectal cancer (CRC). However, the prognostic role of PIK3CA E545K mutation needs to be elucidated. Methods: Tumors from 187 CRC patients were retrospectively collected from the Taleghani and Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran, between 2010 and 2017. PIK3CA E545K status was detected in Formalin-fixed paraffin-embedded (FFPE) tissues using PCR-RFLP methods, and validated by pyrosequencing. Correlations between PIK3CA E545K mutation clinicopathological features were analyzed. Results: The frequency of PIK3CA E545K gene mutations in CRC patients was 10.7%. Significant correlations were observed in PIK3CA E545K mutation with tumor differentiation and TNM stage (p < 0.042 and p = 0.033, respectively). Kaplan-Meier analysis showed a worse prognosis in overall survival (OS) in patients with PIK3CA E545K mutation (p < 0.001). Multivariate analysis indicated that PIK3CA E545K mutation was a detrimental factor for OS (HR = 6.497, 95% CI: 2.859-14.768, p < 0.021). Conclusion: A high frequency of PIK3CA E545K mutation was detected in Iranian CRC patients. The results of the present study suggested that PIK3CA E545K mutation may be associated with poor prognosis. These findings require further confirmation via prospective studies with larger samples. [ABSTRACT FROM AUTHOR]

The human epidermal growth factor 2 (HER2) gene undergoes various mutations that could alter its activity or respond to the antibody therapies. Cetuximab, a known anti‐EGFR monoclonal antibody (mAB), is widely administered in metastatic colorectal cancer (mCRC) cases. Here we identified mCRC patients who did not respond to cetuximab (500 mg/m2, q2w) after fluoropyrimidine/oxaliplatin regimen failure. Tumor samples were examined with immunohistochemistry for protein distribution, polymerase chain reaction (PCR) sequencing for mutation detection and real‐time PCR for mRNA expression pattern analysis between cetuximab sensitive and resistance patients. The conformational differences of normal and mutated protein structures were predicted by bioinformatics analysis. The 5‐year survival rates of target groups were estimated using the Kaplan–Meier method. Immunohistochemistry showed that all cases had high level of HER2 protein. No K‐Ras or B‐Raf mutation was observed among the study population; however, cetuximab resistance patients harbored a somatic mutation R784G at the exon 20 region of HER2 coding sequence. According to bioinformatics analysis, this mutation caused a notable misfold in protein conformation. Meanwhile, survival analysis showed R784G mutated mCRC patients had shortened survival rate compared with the mCRC cases with wild‐type HER2. Collectively, these data report a new mechanism of resistance to cetuximab and might be applicable in modifying new therapeutic strategies for HER2 involved cancers. [ABSTRACT FROM AUTHOR]

In this experiment, at first, waste polyvinyl chloride was used as an additive in an attempt to improve the performance of bitumen and hot mix asphalt (HMA) in order to minimize the amount of rutting. To further these aims, conventional and rheological tests were conducted on bitumen and all HMA specimens were tested by Marshall stability, flow, and repeated load axial tests. After investigating rutting resistance of HMA samples, the results demonstrated that this waste material could improve the rheological properties of bitumen, and the HMA samples, manufactured by this particular type of bitumen, are more resistant to rutting. [ABSTRACT FROM AUTHOR]

Asphaltene precipitation is introduced as a seriously problematic issue in the petroleum reservoirs that causes filling porosity of rocks and reduction of oil production. Hence, estimating the amount of precipitated asphaltene has huge importance in preventing the deposition of asphaltene. The present study was done to estimate the precipitated asphaltene as a function of temperature, dilution ratio, and molecular weight of different n-alkanes using adaptive neuro-fuzzy inference system (ANFIS). Moreover, another new scaling model was also developed to compare with the ANFIS model. In addition, these two developed models have been compared with previously developed correlations. The obtained values of R2for the ANFIS and scaling models were 0.9912 and 0.9862, respectively. These tools are simple to use and can be used as an accurate approximation of the precipitated asphaltene as a function of temperature, dilution ratio, and molecular weight of different n-alkanes. [ABSTRACT FROM PUBLISHER]

The influence of microsatellite instability (MSI) on the prognosis of colorectal cancer (CRC) requires more investigation. We assessed the role of MSI status in survival of individuals diagnosed with primary colorectal cancer. In this retrospective cross-sectional study the MSI status was determined in 158 formalin-fixed paraffin-embedded tumors and their matched normal tissues from patients who underwent curative surgery. Cox proportional hazard modeling was performed to assess the clinical prognostic significance. In this study we found that MSI-H tumors were predominantly located in the colon versus rectum (p=0.03), associated with poorer differentiation (p=0.003) and TNM stage II/III of tumors (p=0.02). In CRC patients with stage II, MSI-L cases showed significantly poorer survival compared with patients who had MSI-H or MSS tumors (p=0.04). This study indicates that MSI-L tumors correlate with poorer clinical outcome in patients with stage II tumors (p=0.04) or in tumors located in the colon (p=0.02). MSI-L characterizes a distinct subgroup of CRC patients who have a poorer outcome. This study suggests that MSI status in CRC, as a clinical prognostic marker, is dependent on other factors, such as tumor stage and location. [ABSTRACT FROM AUTHOR]

Aim: We aimed to explore the frequency of BRAFV600E mutation in Iranian patients with colorectal cancer (CRC) as well as its association with clinic pathological characteristic of patients. Background: CRC is the third leading cause of cancer related death. There is a growing body of data showing the association of BRAFV600E mutation with malignant transformation and clinical outcome of different tumors, including CRC. These findings suggest that BRAFV600E mutation can be used as diagnostic and/or prognostic biomarker for management of cancer patients. Patients and methods: A total of 85 patients with sporadic tumor were recruited. BRAFV600E mutation was investigated using sequencing of extracted DNAs from formalin-fixed paraffin-embedded (FFPE) tumor tissues. Electropherograms were analyzed using Laser-gene 6 software. Results: More than 95% of patients were in stage I and II and none of them were in stage IV. Patients were mostly below 55 years old and tumors were dominantly located in the distal colon. Of note, no BRAFV600E mutations were detected in our population. Conclusion: Our results showed no V600E mutation in the BRAF gene in stage I and II of CRC patients. Further studies in multi-center settings are warranted to examine the prognostic and/or predictive value of this marker in different stages of colorectal cancer patients. [ABSTRACT FROM AUTHOR]

Nowadays, the COVID-19 pandemic has had many harmful effects on the hotel industry. This study aims to identify and prioritize the factors affecting SERVQUAL in the hotel industry and evaluates five-star hotels during the COVID-19 Pandemic. The present study prioritizes the influence factors of SERVQUAL using the stepwise weight assessment ratio analysis (SWARA) method and using the Additive Ratio Assessment (ARAS) method to identify the best hotel among the five hotels in Iran in 2022. In the present study, 22 influence sub-criteria in the hotel industry were studied, and the delivery of service promise sub-criterion was ranked first, which shows its importance among other sub-criteria. Among the hotels studied, A1 hotels ranked first among other hotels. The present study examines and prioritizes the influence factors of SERVQUAL in the hotel industry to help hotel managers to identify the most important influence factors of SERVQUAL in the hotel industry to implement these factors in their hotels and can improve their strengths. [ABSTRACT FROM AUTHOR]