14 results on '"Takehiro Sejima"'
Search Results
2. Site-dependent differences in the composite fibers of male pelvic plexus branches: an immunohistochemical analysis of donated elderly cadavers
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Kuniyasu Muraoka, Shuichi Morizane, Keisuke Hieda, Masashi Honda, Takehiro Sejima, Gen Murakami, Shin-ichi Abe, and Atsushi Takenaka
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Pelvic autonomic nerve plexus ,Fiber composition ,Neuronal nitric oxide synthase ,Vasoactive intestinal polypeptide ,Tyrosine hydroxylase ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Background Although the pelvic autonomic plexus branches are considered to be a mixture of sympathetic and parasympathetic nerves, little is known regarding the composite fibers of the pelvic plexus branches. This study aimed to investigate the immunohistochemical features of sympathetic and parasympathetic nerves in the pelvic autonomic plexus branches. Methods Using 10 donated elderly male cadavers, the detailed topohistology of nerve fibers at and around the bladder, seminal vesicle, prostate, and rectum was examined. Neuronal nitric oxide synthase (nNOS) and vasoactive intestinal polypeptide (VIP) were used as parasympathetic nerve markers; tyrosine hydroxylase (TH) was used as a sympathetic nerve marker. The myenteric plexus of the colon was utilized as a positive control. Results Most nerve fibers in the bladder, seminal vesicle, prostate, and rectum were both nNOS- and TH-positive. Thus, pelvic plexus branches were classified into two types: 1) triple-positive mixed nerves (nNOS+, VIP+, TH+, thick myelinated fibers + or -) and 2) double-positive mixed nerves (nNOS+, VIP-, TH+, thick myelinated fibers + or -). Notably, triple-positive nerves were localized within the posterosuperior part of the plexus (near the rectum) and travelled anteroinferiorly toward the posterolateral corner of the prostate. The posteriorly and inferiorly located nerves were predominantly composed of parasympathetic, rather than sympathetic, fibers. In contrast, nerve fibers within and along the bladder and seminal vesicle contained either no or few VIP-positive nerves. These superiorly located nerves were characterized by clear sympathetic nerve dominance. Conclusions The nerves of the pelvic plexus branches were clearly classified into nerves around the bladder and seminal vesicle (VIP-negative) and nerves around the prostate (VIP-positive). Although nNOS- and VIP-positive nerve fibers are candidate cavernous nerves, cavernous nerve identity cannot be definitively concluded for these nerves in the periprostatic region.
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- 2018
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3. The Benefits of Adjuvant Chemotherapy for Upper Tract Urothelial Carcinoma Require at Least Three Cycles.
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Tetsuya Yumioka, Shuichi Morizane, Kuniyasu Muraoka, Hirofumi Oono, Tadahiro Isoyama, Naoyuki Sakaridani, Koji Ono, Takehiro Sejima, Hiroyuki Kadowaki, Katsuya Hikita, Masashi Honda, and Atsushi Takenaka
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ADJUVANT chemotherapy ,TRANSITIONAL cell carcinoma ,KIDNEY surgery ,EVIDENCE-based medicine ,TREATMENT effectiveness - Abstract
Background Upper urinary tract urothelial carcinoma (UTUC) is uncommon. In advanced cases, radical nephroureterectomy (RNU) alone is not curative, and recurrence and metastasis are likely to occur. Adjuvant chemotherapy (AC) is an evidence-based treatment. However, the optimal number of AC cycles is not clear. This multicenter study investigated the number of cycles required for the beneficial effects of AC in Japanese patients with UTUC. Methods Patients who were diagnosed with UTUC and underwent RNU at our hospital and affiliated hospitals from January 2010 to September 2020 were included in the study. Patients with pathological T3 or higher or lymph node metastasis were observed or given AC, and their responses were compared. The AC regimens included gemcitabine and cisplatin or carboplatin. Patients were also classified into two groups: the observation and two cycles of AC group and the three to four cycles of AC group. The survival curves for recurrencefree survival (RFS) and cancer-specific survival (CSS) were evaluated using Kaplan-Meier analyses. Results Of the 133 patients enrolled in the study, 24 received 2 cycles of AC, 37 received 3-4 cycles, and 72 were observed only. The 5-year RFS was 67.1% for the 3-4 cycles of AC group and 41.7% for the observation and two cycles of AC group. The 5-year CSS was 72.2% for the 3-4 cycles of AC group and 35.9% for the observation and two cycles of AC group. RFS and CSS were significantly longer in the 3-4 cycles of AC group compared to the observation and 2 cycles group (P = 0.048 and P = 0.005 respectively). Conclusion AC prolonged RFS and CSS in the realworld setting. However, at least three cycles of AC are required to achieve beneficial effects in patients with UTUC. [ABSTRACT FROM AUTHOR]
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- 2024
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4. ISOLATED ADRENOCORTICOTROPIC HORMONE DEFICIENCY ASSOCIATED WITH PEMBROLIZUMAB THERAPY IN A PATIENT WITH ADVANCED URETER CANCER : A CASE REPORT AND LITERATURE REVIEW.
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TOSHIHIKO MASAGO, TAKAHIRO HOSHINO, HIROSHI YAMAGUCHI, TAKEHIRO SEJIMA, KENICHI TAKEDA, and MOTOFUMI SASAKI
- Abstract
Copyright of Nishinihon Journal of Urology is the property of West Japan Urological Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
5. Comprehensive Investigations of Multiple Factors That Are Related to Refractory Outcome in Urosepsis Patients.
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Takehiro Sejima, Toshihiko Masago, Shuichi Morizane, Masashi Honda, and Atsushi Takenaka
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SEPSIS ,URINARY tract infections ,FEVER ,LOGISTIC regression analysis ,C-reactive protein ,SERUM albumin - Abstract
Background Urosepsis is an acute life-threating disease, and some cases show refractory outcome to therapy. In an aging society of developed countries, characteristics of urosepsis are becoming complicated. We performed a comprehensive investigation regarding the clinical and social aspects that are related to refractory outcomes in urosepsis patients. Methods The patient cohort consisted of 66 patients with urosepsis. Multiple factors from clinical and social aspects were reviewed retrospectively. Two categories of refractory outcomes were defined. One was afebrile resistance (AR); fever continued more than 7 days from the initiation of therapy. Another was discharge resistance (DR); hospitalization continued for more than 30 days. Logistic regression analyses were performed to identify significant factors that are related to the AR or DR. Results Univariate analysis demonstrated that high score of Eastern Cooperative Oncology Group Performance Status (ECOG PS) (≥ 2) and Age-adjusted Charlson comorbidity index (CCI) (≥ 4), high serum C-reactive protein (CRP) level (≥ 14.9 mg/dL), and low serum albumin level (≤ 2.26 g/dL) were significantly related to AR. Univariate analysis results also revealed that high score of ECOG PS (≥ 2), high serum creatinine level (≥ 1.54 mg/dL) and vasopressor administration were significantly related to DR. Multivariate analyses demonstrated that low serum albumin level (≤ 2.26g/dL) was the only significant factor that was related to AR. In contrast, high score of ECOG PS (≥ 2) and high serum creatinine level (≥ 1.54 mg/dL) were significant factors that were related to DR. Conclusion It is suggested that evaluating serum albumin levels is essential for the therapeutic first step because hypoalbuminemia was the significant factor that was related to obstruction to antipyresis. It is also suggested that the deterioration of patients' activities of daily living and renal dysfunction might be the refractory factors for discharge from the hospital, which was the ultimate therapeutic goal. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Cross-Sectional Investigations of Pre- and Post-Operative Renal Global Function and Renal Parenchymal Volumetry in Both Partial and Radical Nephrectomy Utilizing Film-Based Technology.
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Takehiro Sejima, Tetsuya Yumioka, Noriya Yamaguchi, Toshihiko Masago, Shuichi Morizane, Katsuya Hikita, Masashi Honda, and Atsushi Takenaka
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- 2018
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7. The Liverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI.
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Ginga Shimakawa, Kimitsune Ishizaki, Shigeyuki Tsukamoto, Moeko Tanaka, Takehiro Sejima, and Chikahiro Miyake
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- 2017
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8. Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition.
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Daisuke Takagi, Shigeo Takumi, Masaki Hashiguchi, Takehiro Sejima, and Chikahiro Miyake
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- 2016
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9. Post-illumination transient O2-uptake is driven by photorespiration in tobacco leaves.
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Takehiro Sejima, Hitomi Hanawa, Ginga Shimakawa, Daisuke Takagi, Yuji Suzuki, Hiroshi Fukayama, Amane Makino, and Chikahiro Miyake
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PLANT photorespiration , *TOBACCO analysis , *MOLECULAR biology , *COMPOSITION of leaves , *PHOTOSYNTHESIS , *ATMOSPHERIC carbon dioxide - Abstract
This study aims to elucidate the molecular mechanism for the transient increase in the O2-uptake rate in tobacco (Nicotiana tabacum cv Xanthi) leaves after turning off actinic lights (ALs). The photosynthetic O2 evolution rate reaches a maximum shortly after the onset of illumination with ALs and then decreases to zero in atmospheric CO2/O2 conditions. After turning off the ALs, tobacco leaves show a transient increase in the O2-uptake rate, the post-illumination transient O2-uptake, and thereafter, the O2-uptake rate decreases to the level of the dark-respiration rate. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], maintained a steady-state value distinct from the photosynthetic O2-evolution rate. In high-[ CO2] conditions, the photosynthetic O2-evolution rate and Y(II) showed a parallel behavior, and the post-illumination transient O2-uptake was suppressed. On the other hand, in maize leaves (a C4 plant), even in atmospheric CO2/O2 conditions, Y(II) paralleled the photosynthetic O2-evolution rate and the post-illumination transient O2-uptake was suppressed. Hypothesizing that the post-illumination transient O2-uptake is driven by C3 plant photorespiration in tobacco leaves, we calculated both the ribulose 1,5-bisphosphate carboxylase- and oxygenase-rates (Vc and Vo) from photosynthetic O2-evolution and the post-illumination transient O2-uptake rates. These values corresponded to those estimated from simultaneous chlorophyll fluorescence/O2-exchange analysis. Furthermore, the H+-consumption rate for ATP synthesis in both photosynthesis and photorespiration, calculated from both Vc and Vo that were estimated from chlorophyll fluorescence/CO2-exchange analysis, showed a positive linear relationship with the dissipation rate of the electrochromic shift signal. Thus, these findings support our hypothesis. [ABSTRACT FROM AUTHOR]
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- 2016
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10. Site-dependent and interindividual variations in Denonvilliers' fascia: a histological study using donated elderly male cadavers.
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Kuniyasu Muraoka, Nobuyuki Hinata, Shuichi Morizane, Masashi Honda, Takehiro Sejima, Gen Murakami, Tewari, Ashutosh K., and Atsushi Takenaka
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DEAD ,IMMUNOHISTOCHEMISTRY ,EOSIN ,SMOOTH muscle ,ACTIN - Abstract
Background: Site-dependent and interindividual histological differences in Denonvilliers' fascia (DF) are not well understood. This study aimed to examine site-dependent and interindividual differences in DF and to determine whether changes in the current approach to radical prostatectomy are warranted in light of these histological findings. Methods: Twenty-five donated male cadavers (age range, 72-95 years) were examined. These cadavers had been donated to Sapporo Medical University for research and education on human anatomy. Their use for research was approved by the university ethics committee. Horizontal sections (15 cadavers) or sagittal sections (10 cadavers) were prepared at intervals of 2-5 mm for hematoxylin and eosin staining. Elastic-Masson staining and immunohistochemical staining were also performed, using mouse monoclonal anti-human alpha-smooth muscle actin to stain connective tissues and mouse monoclonal anti-human S100 protein to stain nerves. Results: We observed that DF consisted of disorderly, loose connective tissue and structures resembling "leaves", which were interlacing and adjacent to each other, actually representing elastic or smooth muscle fibers. Variations in DF were observed in the following: 1) configuration of multiple leaves, including clear, unclear, or fragmented behind the body and tips of the seminal vesicles, depending on the site; 2) connection with the lateral pelvic fascia at the posterolateral angle of the prostate posterior to the neurovascular bundles, being clear, unclear, or absent; 3) all or most leaves of DF fused with the prostatic capsule near the base of the seminal vesicles, and periprostatic nerves were embedded in the leaves at the fusion site; and 4) some DF leaves fused with the prostatic capsule anteriorly and/or the fascia propria of the rectum posteriorly. Conclusions: Site-dependent and interindividual variations in DF were observed in donated elderly male cadavers. All or most DF leaves are fused with the prostatic capsule near the base of the seminal vesicles and some DF leaves are fused with the fascia propria of the rectum posterior. Based on our results, surgeons should be aware of variations and search for them to create a suitable dissection plane to avoid iatrogenic positive margins and rectal injury. [ABSTRACT FROM AUTHOR]
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- 2015
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11. Prostatic ischemia induces ventral prostatic hyperplasia in the SHR; possible mechanism of development of BPH.
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Motoaki Saito, Panagiota Tsounapi, Ryo Oikawa, Shogo Shimizu, Masashi Honda, Takehiro Sejima, Yukako Kinoshita, and Shuhei Tomita
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PROSTATE hypertrophy ,ANIMAL models in research ,HYPERTENSION ,LABORATORY rats ,BLOOD flow ,CARDIOVASCULAR diseases ,MALONDIALDEHYDE ,STANOLONE - Abstract
In the light of increasing evidence that benign prostatic hyperplasia is associated with cardiovascular disease, we have investigated the relationship between prostatic blood flow and prostatic hyperplasia in the spontaneously-hypertensive-rat (SHR). Twelve-week-old male SHRs were treated with nicorandil for six weeks. Wistar-Kyoto rats were used as controls. Six weeks after nicorandil treatment, blood pressure and the prostatic blood flow were estimated, and tissue levels of malondialdehyde, HIF-1α, TGF-β1, bFGF, dihydrotestosterone, and α-SMA were measured. SHRs showed significant increases in blood pressure, tissue levels of malondialdehyde, HIF-1α, TGF-β1, bFGF, α-SMA and a significant decrease in the prostatic blood flow. Although treatment with nicorandil failed to alter the blood-pressure and α-SMA, it significantly ameliorated the increased levels of malondialdehyde, HIF-1α, TGF-β1, and bFGF. There were no significant differences in tissue levels of dihydrotestosterone among any groups. These data indicate that development of prostatic hyperplasia may be associated with prostatic hypoxia, which nicorandil prevents via its effect to increase the blood flow. [ABSTRACT FROM AUTHOR]
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- 2014
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12. Low pre-operative levels of serum albumin predict lymph node metastases and ultimately correlate with a biochemical recurrence of prostate cancer in radical prostatectomy patients.
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Takehiro Sejima, Hideto Iwamoto, Toshihiko Masago, Shuichi Morizane, Akihisa Yao, Tadahiro Isoyama, Hiroyuki Kadowaki, and Atsushi Takenaka
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SERUM albumin , *CANCER patients , *METASTASIS , *PROSTATE cancer , *PROSTATECTOMY - Abstract
Introduction. To date, only few studies focusing on the issue of host general and immune activity have been performed in localized prostate cancer (PCa). The aim of this study was to elucidate potent non tumor-related biomarkers that express aggressiveness of PCa treated by radical prostatectomy (RP). Materials and methods. Data from 179 patients who underwent RP were analyzed. The correlations between various kinds of non tumor-related factors in addition to tumor-related factors and biochemical recurrence (BCR) were analyzed. The correlations between pre-, intra- and post-operative factors were also analyzed. Results. Thirty-two cases (17.9%) had a BCR. The factors found to be significantly predictive of BCR using a Cox-proportional hazard model were the pre-operative serum prostate specific antigen (PSA) level and the existence of pathological lymph node metastasis (LNM). A low pre-operative serum albumin level (<4.0 g/dl) was significantly correlated with BCR univariately. Logistic regression analysis revealed that a low pre-operative serum albumin level, an American Society of Anesthesiologists (ASA) score above class 2, and a Gleason score above 8 in the biopsy specimens were significantly predictive of pathological LNM. Conclusions. Tumor-related characteristics are more important for predicting BCR. However, our results suggest that low pre-operative serum albumin level may indicate extensive disease of clinically localized PCa and may ultimately be correlated with BCR. Although multiple reasons may account for the significance of the serum albumin level, it is noteworthy that delayed diagnostic and therapeutic procedures in comorbid patients with low serum albumin levels may lead to PCa progression. [ABSTRACT FROM AUTHOR]
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- 2013
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13. Alteration of apoptotic regulatory molecules expression during carcinogenesis and tumor progression of renal cell carcinoma.
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Takehiro Sejima, Fahri, Tadahiro Isoyama, Fahri, and Ikuo Miyagawa, Fahri
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RENAL cell carcinoma , *CANCER invasiveness , *POLYMERASE chain reaction , *CARCINOGENESIS - Abstract
Abstract Background: In order to characterize the alteration of apoptotic regulatory molecule expressions during carcinogenesis and tumor progression of renal cell carcinoma (RCC), we compared the expressions between tumor and normal tissues, and evaluated the relationships between expressions in tumors with pathological and clinical characteristics. Methods: Reverse transcription–polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) allowed the determination of Fas, Fas ligand (FasL), and bcl-2 mRNA expressions in surgically resected tissues of 40 cases of RCC . Results: In comparing the mRNA expression incidence between tumor and normal tissues, RT-PCR analysis demonstrated that the Fas and bcl-2 incidence in tumors was significantly lower than that in normal tissues. An IHC analysis was supportive of the RT-PCR results that higher immunoreactivity was recognized in mRNA positive cases than in negative cases. With regard to relationships with pathological and clinical characteristics, FasL mRNA expression was recognized significantly more frequently in pT3a tumors than in pT1 tumors, and also more frequently in G2 tumors than in G1 tumors. A similar result was obtained by IHC analysis. Five patients died of cancer. Four of these patients had tumors which showed positive mRNA expression and a strong immunoreactivity of FasL. Conclusion: It is suggested that the alteration of the down-regulated expression of Fas and bcl-2 might contribute to the carcinogenesis of RCC. It is also suggested that the alteration of up-regulated expression of FasL in tumors, which establishes the immune evasion mechanism in some malignancies, might be characterized during the tumor progression of RCC. [ABSTRACT FROM AUTHOR]
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- 2003
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14. AUTHOR'S REPLY.
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Takehiro Sejima
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PROSTATE cancer ,EXOCRINE glands ,METASTASIS ,BIOMARKERS ,CANCER invasiveness - Abstract
In this article the author comments about views on their article on discovering preoperative non-tumor related biomarkers which can easily be obtained by routine laboratory examination. The author says that delayed diagnostic procedure may be responsible for micro metastases progression of prostate cancer (PCa).
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- 2013
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