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The tissue‐specific heart decellularized extracellular matrix (hdECM) demonstrates a variety of therapeutic advantages, including fibrosis reduction and angiogenesis. Consequently, recent research for myocardial infarction (MI) therapy has utilized hdECM with various delivery techniques, such as injection or patch implantation. In this study, a novel approach for hdECM delivery using a wet adhesive paintable hydrogel is proposed. The hdECM‐containing paintable hydrogel (pdHA_t) is simply applied, with no theoretical limit to the size or shape, making it highly beneficial for scale‐up. Additionally, pdHA_t exhibits robust adhesion to the epicardium, with a minimal swelling ratio and sufficient adhesion strength for MI treatment when applied to the rat MI model. Moreover, the adhesiveness of pdHA_t can be easily washed off to prevent undesired adhesion with nearby organs, such as the rib cages and lungs, which can result in stenosis. During the 28 days of in vivo analysis, the pdHA_t not only facilitates functional regeneration by reducing ventricular wall thinning but also promotes neo‐vascularization in the MI region. In conclusion, the pdHA_t presents a promising strategy for MI treatment and cardiac tissue regeneration, offering the potential for improved patient outcomes and enhanced cardiac function post‐MI. [ABSTRACT FROM AUTHOR]

Hemorrhage is one of the major challenges in healthcare area. Many hemostats are commercially available, but their application is limited due to high cost, non-biocompatible and immunogenic properties. Chitosan and its derivatives have been extensively used as a hemostatic material to stop bleeding. The amine and hydroxyl groups of chitosan are modified into different derivatives, for exhibiting better solubility, hemostatic properties, biocompatibility, moisture absorption, film-forming, and antibacterial activities. One such derivative is carboxymethyl chitosan and it has been widely studied for hemostatic and other biomedical applications. In this book chapter, we are overviewing the different forms of carboxymethyl chitosan derivatives such as hydrogel, sponges, cryogel, membranes and microspheres which were studied for hemostatic applications. [ABSTRACT FROM AUTHOR]

Hemorrhage is the leading cause of death in combat settings, trauma injury, and during surgical procedures. Hemostatic agents and tissue sealants are required when body’s coagulation cascade is not able to control hemorrhage effectively. Chitosan because of its hemostatic and tissue adhesive property has gained popularity as a topical hemostatic agent. Chitosan and derivatives of chitosan in different forms such as sponge, hydrogel, dressing, and particles have been extensively studied as hemostatic agent. This chapter provides an overview of the basic hemostatic property of chitosan and also reviews the recent progress on different forms of chitosan and its derivatives as hemostatic agents and tissue sealants. [ABSTRACT FROM AUTHOR]

Abstract Effective bleeding control is a major concern in trauma and major surgeries. Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug. Our prime interest is to improve the blood clotting property of Ch hydrogel. Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH 2 group) would act at the same time and bring about rapid blood clot formation. Sol-gel method was followed to synthesize nBG particles and its particle size was found to be 14 ± 3 nm. 2%Ch-5%nBG hydrogel was then prepared and studied using SEM and FTIR. The prepared hydrogel was injecable and was also cytocompatible with HUVEC. In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel. Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations. [ABSTRACT FROM AUTHOR]

Periodontitis is an inflammatory disease that causes destruction of tooth-supporting tissues and if left untreated leads to tooth loss. Current treatments have shown limited potential for simultaneous regeneration of the tooth-supporting tissues. To recreate the complex architecture of the periodontium, we developed a bilayered construct consisting of poly(caprolactone) (PCL) multiscale electrospun membrane (to mimic and regenerate periodontal ligament, PDL) and a chitosan/2wt % CaSO4 scaffold (to mimic and regenerate alveolar bone). Scanning electron microscopy results showed the porous nature of the scaffold and formation of beadless electrospun multiscale fibers. The fiber diameter of microfiber and nanofibers was in the range of 10 ± 3 µm and 377 ± 3 nm, respectively. The bilayered construct showed better protein adsorption compared to the control. Osteoblastic differentiation of human dental follicle stem cells (hDFCs) on chitosan/2wt % CaSO4 scaffold showed maximum alkaline phosphatase at seventh day followed by a decline thereafter when compared to chitosan control scaffold. Fibroblastic differentiation of hDFCs was confirmed by the expression of PLAP-1 and COL-1 proteins which were more prominent on PCL multiscale membrane in comparison to control membranes. Overall these results show that the developed bilayered construct might serve as a good candidate for the simultaneous regeneration of the alveolar bone and PDL. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 761-770, 2016. [ABSTRACT FROM AUTHOR]