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The article explores the use of sediment DNA (sedaDNA) in archaeology and its potential to provide valuable insights into ancient organisms and their interactions. It emphasizes the importance of understanding the context and association of sedaDNA within the archaeological record. The authors propose a microcontextual approach to sedaDNA studies, which involves analyzing the microscopic sources and movement of sedaDNA to ensure reliable collection and interpretation of data. They also highlight the need for external validation and a thorough understanding of the formation history of sedaDNA. By integrating sedaDNA analysis with stratigraphic and geological studies, researchers can enhance their understanding of sedaDNA hotspots and improve the interpretation of genetic data. [Extracted from the article]

Over the last two decades, much of the recent efforts dedicated to the Levantine Middle Paleolithic has concentrated on the role of open-air sites in the settlement system in the region. Here focus on the site of ‘Ein Qashish as a cases study. Located in present-day northern Israel, the area of this site is estimated to have been >1300 m2, of which ca. 670 were excavated. The site is located at the confluence of the Qishon stream with a small tributary running off the eastern flanks of the Mt. Carmel. At the area of this confluence, water channels and alluvial deposits created a dynamic depositional environment. Four Archaeological Units were identified in a 4.5-m thick stratigraphic sequence were dated by Optically Stimulated Luminescence (OSL) to between—71 and 54 ka, and probably shorter time span–~70-~60 ka. Here we present the diverse material culture remains from the site (lithics, including refitted sequences; modified limestone pieces; molluscs; faunal remains) against their changing paleogeographic backdrop. Skeletal evidence suggests that these remains were associated with Neanderthals. The large-scale repeated accumulation of late Middle Paleolithic remains in the same place on the landscape provides a unique opportunity to address questions of occupation duration and intensity in open-air sites. We find that each occupation was of ephemeral nature, yet presents a range of activities, suggesting that the locale has been used as a generalized residential site rather than specialized task-specific ones. This role of ‘Ein Qashish did not change through time, suggesting that during the late Middle Paleolithic settlement system in this part of the southern Levant were stable. [ABSTRACT FROM AUTHOR]

Archaeological investigations undertaken by the Proyecto Arqueológico Tlajinga Teotihuacán are focused on understanding urban expansion and household economies in this southern district of the city. Our geoarchaeological research addresses similar topics through examination of relevant microstratigraphic and botanical signatures as well as those relevant to reconstructing paleoenvironment. We investigated four different contexts at Tlajinga: the southern extension of the Street of the Dead, an obsidian working area outside Compound 17:S3E1, anthropogenic and natural layers below Compound 18:S3E1, and a soil profile at the San Lorenzo river. We employed micromorphological, pollen, and phytolith analyses as well as standard soil analytics to study the various deposits in these contexts. Our analysis demonstrates artificial lowering of the tepetate for continuing the axis of the Street of the Dead, microdebitage from obsidian working outside Compound 17:S3E1, and the preservation of in situ burning activities at Compound 18:S3E1. Further, we reconstruct alluvial infilling of the Street of the Dead with pedogenetic overprinting and present further evidence on the occurrence and variability of the Black San Pablo Paleosol and its agricultural significance. [ABSTRACT FROM AUTHOR]

The resilience of Neanderthals towards changing climatic and environmental conditions, and especially towards severely cold climates in northern regions of central Europe, is still under debate. One way to address this is to investigate multi-layered occupation in different climatic intervals, using independently-compiled paleoenvironmental and chronological data. Unfortunately, most open-air sites on the northern European Plain lack a robust chronostratigraphy beyond the radiocarbon dating range, thereby often hampering direct links between human occupation and climate. Here we present the results of integrative research at the Middle Paleolithic open-air site of Lichtenberg, Northern Germany, comprising archaeology, luminescence dating, sedimentology, micromorphology, as well as pollen and phytolith analyses. Our findings clearly show Neanderthal presence in temperate, forested environments during the Mid-Eemian Interglacial, MIS 5e and the latest Brörup Interstadial, MIS 5c/GI 22 (Lichtenberg II). For the previously known occupation Lichtenberg I, we revise the chronology from the former early MIS 3 (57 ± 6 ka) to early MIS 4/GS 19 (71.3 ± 7.3 ka), with dominant cold steppe/tundra vegetation. The early MIS 4 occupation suggests that Neanderthals could adjust well to severely cold environments and implies recurring population in the region between MIS 5 and MIS 3. The artefact assemblages differ between the temperate and cold environment occupations regarding size, blank production, typology and tool use. We argue that this distinctness can partially be explained by different site functions and occupation duration, as well as the availability of large and high-quality flint raw material. Raw material availability is in turn governed by changing vegetation cover that hindered or fostered sediment redeposition as a provider of flint from the primary source of the glacial sediments nearby. • Integrative multidisciplinary approach of geo-biosciences and archaeology. • Precise chronology connects Neanderthal occupation to distinct climatic phases. • Neanderthals adapted to changing and also severely cold environments in northern Central Europe. • Variability of tool size, raw material and tool use in response to climate changes. • First numerical dates for early Weichselian interstadials in the type area. [ABSTRACT FROM AUTHOR]