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Abstract: The work presents a new and highly sensitive disposable amperometric triglyceride (TG) biosensor based on Au/PEDOT-PSS nanocomposite inkjet-printed on screen-printed carbon electrodes (SPCEs) and co-immobilization of lipase, glycerol kinase and glycerol-3-phosphate oxidase. One-step chemically synthesized Au/PEDOT-PSS nanocomposite deposited by inkjet printing is demonstrated to produce in nanorod structures on SPCE surface. The electrochemical responses are found to be significantly enhanced by Au nanoparticles and optimal preparation conditions of gold nanoparticle formation for electrochemical detection are found to be 0.015M EDOT and 6.25mM gold precursor. For TGs determination in blood, SPCE electrode with 5 layers of inkjet printed Au/PEDOT-PSS nanocomposite provides optimum performances when operated with the operating potential of 0.4V in 0.1M sodium phosphate buffer pH of 7.0. Wide dynamic range (0–531mg/dL), moderate sensitivity (2.66μA/mM), modest response time (30s), low detection limit (7.88mg/dL), low interference, good reproducibility and satisfactory life time (40% loss of response current after 30 days of storage at 4°C) have been achieved. Therefore, inkjet-printed Au/PEDOT-PSS nanocomposite on SPCEs is a promising candidate for TGs determination. [Copyright &y& Elsevier]