Search

Context. Dietary supplementation with phytobiotics may form part of an alternative strategy to the use of antibiotics as growth promoters in the poultry industry. Essential oils of rosemary (Rosmarinus officinalis L.) may have antimicrobial and antioxidant properties. Aims. The aims were to evaluate in vitro characteristics of non-encapsulated and encapsulated rosemary essential oils, and to investigate their effects on growth performance, intestinal morphology and microbial population in broiler chickens. Methods. Essential oil main components were determined, and the minimum inhibitory concentration and 2,2-diphenyl-1-picrylhydrazyl radical assay methods were applied for determination of antimicrobial and antioxidant activities, respectively. In an in vivo study of performance, 1-day-old male chicks (n = 420) were randomly assigned to seven dietary treatments with five replicates of 12 birds in a completely randomised experimental design. Treatments were control (basal) diet, and basal diet supplemented with chitosan (encapsulating agent) at 150 mg/kg, encapsulated and non-encapsulated rosemary essential oils at both 150 and 300 mg/kg, and the antibiotic flavophospholipol at 650 mg/kg. Key results. Non-encapsulated essential oil had stronger antioxidant activity than the encapsulated form (IC50 values 0.969 and 1.879 mg/mL, respectively). Non-encapsulated essential oil had greater antimicrobial effect against Escherichia coli, whereas the encapsulated form was more effective against Staphylococcus aureus. During the whole growing period of the in vivo study, supplementation with different levels of non-encapsulated or encapsulated essential oils significantly (P < 0.01) improved bodyweight gain and feed conversation ratio compared with control and antibiotic diets. Furthermore, feed intake in birds receiving essential oils (150 or 300 mg/kg) was lower (P < 0.01) than in other groups. Villus height was increased (P < 0.01) in birds supplemented with essential oils compared with all other treatments. Ileal coliform count was lower in birds supplemented with non-encapsulated essential oil at 150 mg/kg than in birds fed the control diet. Conclusion. Use of non-encapsulated rosemary essential oils at 150 mg/kg is recommended. Encapsulation did not provide additional benefits for most parameters. Implications. Rosemary essential oils offer a potential replacement to conventional antibiotics in poultry nutrition, reducing the spread of antibiotic-resistant bacteria and presence of antibiotic residues in animal tissues. [ABSTRACT FROM AUTHOR]

One experiment was carried out to investigate the effect of low crude protein (CP) diets supplemented with crystalline essential amino acids (EAAs) on growth performance, meat quality, and nitrogen retention in Japanese quails reared from 1 to 35 days of age. A total of 840 unsexed one-day-old quail chicks were used in a completely randomized design consisting of seven treatments with six replicates and 20 birds in each. In addition to the control diet, two negative control diets were also adjusted to contain 10 and 20% lower CP than the control (LP10 and LP20 diets, respectively). The EAAs/CP ratio was similar to that of the control diet. Also, four rations supplemented with amino acids were formulated to contain 10% (LP10+EAAs10 and LP20+EAAs10) or 20% (LP10+EAAs20 and LP20+EAAs20) higher EAAs than LP10 and LP20. Crude protein digestibility was determined using the total collection method at 21 and 35 d of age. After slaughtering at 21 and 35 d of age, whole-breast meats were dissected out to determine meat quality indices. During 1 - 21d of age, 10 or 20% reduction in CP significantly decreased body weight gain and increased feed conversion ratio; however, amino acid supplementation significantly improved body weight gain and feed conversion ratio (P < 0.05). During 1- 35 d of age, reduction of CP to 20% led to decreased body weight gain in EAAs supplemented and un-supplemented diets. Dietary treatments did not have any significant effect on the breast meat quality variables except for pH. At 35d of age, 20% reduction in dietary CP significantly reduced nitrogen retention percentage. Overall, a 10% reduction in dietary CP without EAAs supplementation had no adverse effect on the growth performance of Japanese quails during the 1 to 35d of age. [ABSTRACT FROM AUTHOR]