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Objective: To evaluate the efficacy of silver diamine fluoride (SDF) in arresting dentin caries lesions when applied under different concentrations and times. Material and Methods: Forty-two bovine blocks were selected and fixed in 24-well plates. Each well received a mixed bacterial inoculum added to the culture medium with 5% sucrose. The plates were incubated in microaerophilia (7 days) for caries formation, confirmed by micro-CT (M1). SDF was applied over the carious lesions for different times and concentrations (n=6): SDF 30% - immediate removal, 1 minute and 3 minutes; SDF 38%, - immediate removal, 1 minute and 3 minutes. The group without treatment was the control. Then, the samples were again scanned by micro-CT (M2) and submitted to a second cariogenic challenge for 21 days. Then, a final scan was performed (M3). Results: Mean pH at the culture medium and lesion depth were compared using Kruskal-Wallis and Wilcoxon tests. 38% SDF showed the lowest metabolic activity of the biofilm. All 38% groups and 30% 1 and 3 minutes did not show an increase in mean lesion depth comparing M3 with M1. However, only 30% 3 minutes and 38% 1 and 3 minutes showed a significant reduction of lesion depth. Conclusion: The minimum application time of 30% SDF to arrest dentin caries lesion was 1 minute, while 38% SDF arrested with application and immediate removal.

Abstract This study aimed to evaluate the endodontic instrumentation outcomes with asymmetrical files compared to reciprocating and hand files (HFs) in 3D-printed prototypes of upper primary incisors using micro-computed tomography (micro-CT). For this purpose, 50 prototypes were randomly divided (n = 10) according to the instrumentation technique as follows: HFs, a reciprocating file (WaveOne® Gold [WOG]), and three asymmetrical movement files: XP-Endo® Shaper (XPS), XP-Endo® Finisher (XPF), and XP Clean (XPC). The specimens were scanned and, after registration of the baseline and instrumented volumes, changes in the root canal volume (RCV), debris accumulation, removed root material volume (RRMV), non-instrumented areas, and the presence of cracks/perforations were quantified. Data were analyzed by analysis of variance and Student’s t-test, while the effect size was calculated for statistically significant outcomes. All groups showed an increase in RCV after instrumentation (p < 0.05), but this was higher with HFs (p < 0.05). Accumulated debris was higher for WOG and XPS (p < 0.05), but WOG exhibited more in the medium and apical third areas. HFs showed the highest RRMV (p < 0.05), especially at the apical third. The non-instrumented areas were lower for HFs and XPC than for the other systems (p < 0.05). Cracks were present in a few WOG (n = 2) and HF specimens (n = 3) and in this group, one of the cracked specimens and two others showed perforations. The asymmetric systems resulted in conservative dentin removal and fewer cracks/perforations as compared to HFs and a reciprocation file in prototyped primary upper incisors. XPC showed the best compromise between RRMV and non-instrumented areas with a low accumulation of debris.

Background: Eccentric instruments have been proposed as more effective and less time‐consuming for endodontic instrumentation. Aim: To compare biomechanical outcomes of different instrumentation systems and time undertaken for instrumentation in resin prototypes. Design: Sixty standardized prototypes of mandibular second primary molars were instrumented according to the following systems: K‐files, ProTaper Next (PTN), XP‐endo Shaper (XPS), XP‐endo Finisher (XPF), XP–Clean (XPC), and Sequence Baby File (SBF; n = 10/each). Irrigation was performed with saline with simultaneous aspiration, and time spent was recorded. The prototypes were micro‐CT‐scanned before and after the instrumentation, and image sets were reconstructed and registered. Non‐instrumented areas, accumulated debris, removed root material volume, and canal transportation were quantified. Data were analyzed through ANOVA, the Kruskal–Wallis test, and the Wilcoxon signed‐rank test (α = 5%). Results: K‐files and SBF resulted in more instrumentation time (p <.05). SBF, XPC and PTN removed less root dentine (p <.05), but PTN left more untouched areas (p <.05). Accumulated debris were lower for XPC and SBF (p <.05). Canal transportation was similar among the groups. Conclusion: Rotary systems reduced instrumentation time, whereas SBF and XPC resulted in more conservative instrumentation, with less debris accumulation and non‐instrumented areas. A dedicated paediatric endodontic system (SBF) outperformed eccentric instruments in terms of effectiveness. [ABSTRACT FROM AUTHOR]

Introduction This study aimed to compare the effectiveness of two endodontic cleaning techniques, passive ultrasonic irrigation (PUI) and the XP-endo Finisher R (XPR) system, in removing residual filling material during endodontic retreatment procedures. Materials and Methods: Forty mandibular premolars with oval canals were divided into four groups based on the sealer used (AH-Plus or Bio-C Sealer) and the cleaning technique employed (PUI or XPR). To ensure uniformity of canal volume among groups, initial micro-CT scans were conducted. The canals were instrumented, filled, and then re-instrumented before undergoing either PUI or XPR cleaning techniques. Residual filling material volumes were assessed through micro-CT scans, and statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests. Results: Following instrumentation, there was no significant difference in residual filling material volumes between AH-Plus and Bio-C Sealer groups (1.35 mm³ and 1.02 mm³, respectively; P>0.05). However, after supplementary cleaning techniques, XPR-cleaned specimens exhibited significantly less residual material compared to PUI-cleaned specimens (0.01 mm³ and 0.29 mm³ for Bio-C Sealer, and 0.07 mm³ and. 0.30 mm³ for AH-Plus, P<0.05). Conclusion: The XPR system was found to be more effective than PUI in removing residual filling material from Bio-C Sealer-filled root canals. This highlights its potential as a useful supplementary cleaning technique in endodontic retreatment procedures. [ABSTRACT FROM AUTHOR]

This study aimed at evaluating the mineral variation induced by polyalkenoate cements in residual dentine after different caries removal methods. Ten extracted sound molars were selected, and five cavities were prepared on the occlusal surface of each specimen. Carious lesions were generated using a microbial protocol, while the caries removal was performed using bur, hand excavator, or two papain-based chemo-mechanical agents. One cavity was left unexcavated in each tooth. Cavities were restored using a zinc polycarboxylate cement (ZPC; n = 25), or a glass ionomer cement (GIC) was used (n = 25). Subsequently, the specimens were stored under simulated pulpal pressure for 45 days. Micro-CT scanning was performed after caries formation, removal, and restoration. Cavity volumes (mm3) and mineral density (MD) changes were calculated. Remineralisation ability was investigated by scanning electron microscopy (SEM) and Fourier-transformed infrared spectroscopy (FTIR). Statistical analysis was performed (5% significance level). Baseline caries, cavity volume, and MD increase after caries removal were similar to all excavation groups (p > 0.05). MD was higher in ZPC compared to GIC (p < 0.05). SEM revealed that the chemo-mechanical techniques produced a smoother dentine surface compared to mechanical methods. FTIR showed silicon-based minerals in GIC-treated dentine, while ZPC-treated presented mineral deposits characterised by amorphous calcium phosphate. In both cases, the presence of carbonates was detected on dentine surfaces. All caries removal techniques performed similarly in terms of carious dentine excavation. ZPC can be used as dentine replacement material after caries removal as it may induce higher MD increase in residual dentine via deposition of calcium phosphates, compared to GIC. [ABSTRACT FROM AUTHOR]

In this study, an analysis is made of the process of adapting and standardising the Luria-Christense neuropsychological set of tests for use by Brazilian children, teenagers and adults. The process is characterised by research motivated by the need to develop diagnostic assessment instruments standardised to Brazilian social and cultural reality. The set of tests is comprised of ten sets of tests, each one of which assesses part of the dynamic functioning of the brain, thus providing a qualitative mapping of the brain areas and their interlinking, by means of testing perceptual, cognitive and movement abilities. The task of adaptation and standardization is divided into 4 phases. Phase 1 is dedicated to the process of the translation of, changes to and adaptation of signs, pictures and other stimuli, as well as the elaboration of instructions regarding their application and answer form, and also the layout and desktop printing thereof. In Phase 2, the test is taken to the field for the first time, and applied to control and experimental groups, passing, after analysis and alterations, to Phase 3. In Phase 3 the test is taken to the field for the second time, once more undergoing analysis and final alterations. In this way, only those tests that, after qualitative analysis and statistical treatment, still require further revision and use in the field, are subject to Phase 4. The results obtained indicate when the pilot phase of the study can be finished, as follows: no tests in Phase 1 (pre-adaptation), 03 tests in Phase 2, 07 tests in Phase 3 and no tests in Phase 4. Once this research has been fully implemented it will be possible to obtain a more accurate assessment that is more appropriate to Brazilian reality. Keywords: Neuropsychological Assessment; Test Adaptation and Standardisation; Brain Function Mapping.

Objective: To evaluate the efficacy of silver diamine fluoride (SDF) in arresting dentin caries lesions when applied under different concentrations and times. Material and Methods: Forty-two bovine blocks were selected and fixed in 24-well plates. Each well received a mixed bacterial inoculum added to the culture medium with 5% sucrose. The plates were incubated in microaerophilia (7 days) for caries formation, confirmed by micro-CT (M1). SDF was applied over the carious lesions for different times and concentrations (n=6): SDF 30% - immediate removal, 1 minute and 3 minutes; SDF 38%, - immediate removal, 1 minute and 3 minutes. The group without treatment was the control. Then, the samples were again scanned by micro-CT (M2) and submitted to a second cariogenic challenge for 21 days. Then, a final scan was performed (M3). Results: Mean pH at the culture medium and lesion depth were compared using Kruskal-Wallis and Wilcoxon tests. 38% SDF showed the lowest metabolic activity of the biofilm. All 38% groups and 30% 1 and 3 minutes did not show an increase in mean lesion depth comparing M3 with M1. However, only 30% 3 minutes and 38% 1 and 3 minutes showed a significant reduction of lesion depth. Conclusion: The minimum application time of 30% SDF to arrest dentin caries lesion was 1 minute, while 38% SDF arrested with application and immediate removal. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the endodontic instrumentation outcomes with asymmetrical files compared to reciprocating and hand files (HFs) in 3D-printed prototypes of upper primary incisors using micro-computed tomography (micro-CT). For this purpose, 50 prototypes were randomly divided (n = 10) according to the instrumentation technique as follows: HFs, a reciprocating file (WaveOne® Gold [WOG]), and three asymmetrical movement files: XP-Endo® Shaper (XPS), XP-Endo® Finisher (XPF), and XP Clean (XPC). The specimens were scanned and, after registration of the baseline and instrumented volumes, changes in the root canal volume (RCV), debris accumulation, removed root material volume (RRMV), non-instrumented areas, and the presence of cracks/perforations were quantified. Data were analyzed by analysis of variance and Student's t-test, while the effect size was calculated for statistically significant outcomes. All groups showed an increase in RCV after instrumentation (p < 0.05), but this was higher with HFs (p < 0.05). Accumulated debris was higher for WOG and XPS (p < 0.05), but WOG exhibited more in the medium and apical third areas. HFs showed the highest RRMV (p < 0.05), especially at the apical third. The non-instrumented areas were lower for HFs and XPC than for the other systems (p < 0.05). Cracks were present in a few WOG (n = 2) and HF specimens (n = 3) and in this group, one of the cracked specimens and two others showed perforations. The asymmetric systems resulted in conservative dentin removal and fewer cracks/perforations as compared to HFs and a reciprocation file in prototyped primary upper incisors. XPC showed the best compromise between RRMV and non-instrumented areas with a low accumulation of debris. [ABSTRACT FROM AUTHOR]

Background: Achieving a void‐free root canal filling is a challenge in current clinical pulpectomy practice. Aim: To compare filling effectiveness and internal voids of root filling materials and obturation techniques in resin‐prototyped primary incisors by micro‐CT. Design: Fifty prototypes were instrumented and randomly divided (n = 10) according to the filling materials (Vitapex®, ZOE, Calcicur®, Feapex, and Calen®‐ZO) and obturation technique: lentulo or pressure syringe (n = 5). The specimens were micro‐CT scanned (pixel size = 7.83 μm) and reconstructed, and the total volume of filled canal (%FC) and internal voids (%IV) of the bulk filling material was calculated. Kruskal‐Wallis and Mann‐Whitney tests were used to detect differences and interactions among groups. Results: Calen®‐ZO, Vitapex®, and ZOE showed similar %FC. Calcicur® showed lower %FC compared with Calen®‐ZO, Vitapex®, and ZOE (P <.05) but similar to Feapex. No statistically significant differences in %FC between lentulo or pressure syringe were disclosed. Regarding %IV, Vitapex®, and Calen®‐ZO performed better with syringe, although Calcicur® was better with lentulo. The number of voids in the obturation bulk was similar among materials, but always lower (P <.05) in syringe filled specimens. Conclusion: Vitapex®, Calen®‐ZO, and ZOE outperformed Calcicur® and Feapex in %FC and if the syringe technique is used, %IV of the obturation bulk and presence of defects was lower. [ABSTRACT FROM AUTHOR]

To evaluate the microbiologic and tissue action of light emitting diodes (LEDs) in induced bisphosphonate-related osteonecrosis in rats, comparing it with photodynamic therapy with a low-power laser. Ten rats were used, divided into the following groups: G1, animals treated with saline solution; G2, animals treated with antibiotic therapy; G3, animals treated for 14 days with application of a low-intensity laser (660 nm) and methylene blue; G4, animals treated with application of LEDs and methylene blue. Clinical, bacterial, tomographic, and microscopic analyses were performed. Clinically, rats from the laser and LED groups showed a more favorable result for bone exposure management and none of the rats had a local inflammatory process. In the bacterial and tomographic analyses, there was no statistical difference between the 4 groups after treatment. Microscopically, all mandibles had devitalized bone; however, group 4 had a greater amount of bone trabeculae than the other groups, as well as fewer average osteoclasts per area. We conclude that all rats developed osteonecrosis; however, photodynamic therapy with LED did not show a statistical difference when compared to laser and control groups. [ABSTRACT FROM AUTHOR]