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4 results on '"SDV:BBM"'

1. Human Immunodeficiency Virus Protease Inhibitors Accumulate into Cultured Human Adipocytes and Alter Expression of Adipocytokines

Author

Cécile Vernochet, Stéphane Azoulay, Christian Dani, Roger Guedj, Hubert Vidal, Gérard Ailhaud, Daniele Duval, Françoise Cottrez, Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Laboratoire de Chimie des Molécules Bioactives et des Arômes (LCMBA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Interactions cellulaires en immunologie et immunopathologie, Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanisme Moléculaire du Diabète (MMD), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), and Oillaux, Genevieve

Subjects
Adipocytes, Cell Differentiation, Chromatography, High Pressure Liquid, Cytokines, Enzyme-Linked Immunosorbent Assay, HIV Protease Inhibitors, Humans, Insulin Resistance, Reverse Transcriptase Inhibitors, Spectrophotometry, Ultraviolet, Adipose tissue, Biochemistry, chemistry.chemical_compound, MESH: Spectrophotometry, Ultraviolet, Adipocyte, HIV Protease Inhibitor, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Chromatography, High Pressure Liquid, MESH: Cytokines, 0303 health sciences, Biologie du développement, MESH: Enzyme-Linked Immunosorbent Assay, virus diseases, Lopinavir, Development Biology, 3. Good health, MESH: Insulin Resistance, Adipogenesis, medicine.drug, MESH: Cell Differentiation, medicine.medical_specialty, Biology, 03 medical and health sciences, Amprenavir, Internal medicine, [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, SDV:BBM, medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Chromatography, High Pressure Liquid, Molecular Biology, MESH: Adipocytes, MESH: HIV Protease Inhibitors, 030304 developmental biology, MESH: Humans, 030306 microbiology, Cell Biology, Endocrinology, chemistry, Spectrophotometry, Ultraviolet, Ritonavir, MESH: Reverse Transcriptase Inhibitors, Saquinavir
Abstract

International audience; Lipodystrophic syndrome is a major side effect of highly active antiviral therapy. Fat tissue redistribution is associated with changes in adipocyte gene expression and in circulating levels of adipocytokines involved in the development of insulin resistance. However, the evidence that HIV drugs accumulate into human adipocytes and have a direct effect on the expression of adipocyte-specific genes is still lacking. To address these questions, we used adipocytes derived from adult stem (hMADS) cells isolated from human adipose tissue. We showed by ELISA that two inhibitors of the HIV protease, lopinavir and ritonavir, accumulated at similar levels during the development of hMADS cells in adipocytes, whereas a non-nucleoside reverse transcriptase inhibitor, the nevirapine, accumulated at lower levels. Two fluorescent protease inhibitors then have been generated to investigate their subcellular localization. The data showed that HIV drugs accumulated into adipocytes and displayed various effects on hMADS cell-derived adipocytes. Indinavir, amprenavir, and nevirapine did not alter differentiation of precursor cells. In contrast, lopinavir, saquinavir, and ritonavir inhibited the development of preadipocytes into adipocytes. In adipocytes, amprenavir increased leptin expression and ritonavir was able to up-regulate tumor necrosis factor-alpha, interleukin 6, and leptin expression and down-regulate the expression of peroxisome proliferator-activated receptor gamma and adiponectin. Intracellular accumulation and localization of HIV drugs into human adipocytes strongly suggest that adipose tissues store these drugs. Because ritonavir can alter the expression of insulin resistance-related cytokines in human adipocytes in a way parallel to the situation observed in vivo upon treatment of HIV-infected patients, we propose that protease inhibitors participate in insulin resistance through a direct effect on adipocytes.

Published
2005
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2. A simple and efficient method for the long-term preservation of plant cell suspension cultures

Author

Corinne Rivasseau, Anne-Marie Boisson, Richard Bligny, Elisabeth Gout, Laboratoire de physiologie cellulaire végétale (LPCV), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
0106 biological sciences, phosphate starvation, Programmed cell death, Arabidopsis thaliana, Plant cell suspension, Cell, plant, cell culture, method, cell suspension, cell preservation, Acer pseudoplatanus, in vitro, in vivo, NMR, nuclear magnetic resonance, low temperature, Plant Science, lcsh:Plant culture, 01 natural sciences, Suspension culture, 03 medical and health sciences, In vivo, Botany, SDV:BBM, Genetics, medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, lcsh:SB1-1110, lcsh:QH301-705.5, 030304 developmental biology, 0303 health sciences, biology, Cell growth, Methodology, biology.organism_classification, Plant cell, Cell biology, medicine.anatomical_structure, in vitro and in vivo NMR spectroscopy, lcsh:Biology (General), Cell culture, 010606 plant biology & botany, Biotechnology
Abstract

Background The repeated weekly subculture of plant cell suspension is labour intensive and increases the risk of variation from parental cells lines. Most of the procedures to preserve cultures are based on controlled freezing/thawing and storage in liquid nitrogen. However, cells viability after unfreezing is uncertain. The long-term storage and regeneration of plant cell cultures remains a priority. Results Sycamore (Acer pseudoplatanus) and Arabidopsis cell were preserved over six months as suspensions cultures in a phosphate-free nutrient medium at 5°C. The cell recovery monitored via gas exchange measurements and metabolic profiling using in vitro and in vivo 13C- and 31P-NMR took a couple of hours, and cell growth restarted without appreciable delay. No measurable cell death was observed. Conclusion We provide a simple method to preserve physiologically homogenous plant cell cultures without subculture over several months. The protocol based on the blockage of cell growth and low culture temperature is robust for heterotrophic and semi-autotrophic cells and should be adjustable to cell lines other than those utilised in this study. It requires no specialized equipment and is suitable for routine laboratory use.

Published
2012
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3. The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver

Author

Grégory Chevillard, Valérie Nicolas-Francès, Stéphane Mandard, F. Hansmannel, Frank J. Gonzalez, J. A. Bonzo, Thierry Pineau, Stephen A. Duncan, Michele A. Battle, Pascal G.P. Martin, J. Chamouton, Norbert Latruffe, Marie Claude Clémencet, Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Epidémiologie des maladies chroniques : impact des interactions gène environnement sur la santé des populations, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Laboratory of Metabolism, Division of Basic Sciences, National Cancer Institute [Bethesda] (NCI-NIH), National Institutes of Health [Bethesda] (NIH)-National Institutes of Health [Bethesda] (NIH), Lady Davis Institute for Medical Research [Montréal], McGill University = Université McGill [Montréal, Canada]-Jewish General Hospital, Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Laboratoire de Pharmacologie et Toxicologie, UR66, INRA, Institut National de la Recherche Agronomique (INRA), The Ministry of Research, National Education and Technology, the Regional Council of Burgundy, the GDR-CNRS no2583 on peroxisome, the French Ministry of Research, National Education and Technology., McGill University-Jewish General Hospital, Mandard, Stéphane, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Lille, Droit et Santé, National Cancer Institute ( NIH ), Lady Davis Institute for Medical Research, McGill University, and Institut National de la Recherche Agronomique ( INRA )

Subjects
Article Subject, Response element, Peroxisome proliferator-activated receptor, Biology, 03 medical and health sciences, Transactivation, 0302 clinical medicine, Drug Discovery, Gene expression, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, SDV:BBM, Pharmacology (medical), [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, lcsh:QH301-705.5, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Transcription factor, 030304 developmental biology, [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, chemistry.chemical_classification, Genetics, Endocrinology and metabolism, 0303 health sciences, Thiolase, Intron, [ SDV.MHEP.EM ] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Cell biology, lcsh:Biology (General), Nuclear receptor, chemistry, 030220 oncology & carcinogenesis, Endocrinologie et métabolisme, Research Article
Abstract

PPARα and HNF4α are nuclear receptors that control gene transcription by direct binding to specific nucleotide sequences. Using transgenic mice deficient for either PPARα or HNF4α, we show that the expression of the peroxisomal3-keto-acyl-CoA thiolase B(Thb) is under the dependence of these two transcription factors. Transactivation and gel shift experiments identified a novel PPAR response element within intron 3 of theThbgene, by which PPARα but not HNF4α transactivates. Intriguingly, we found that HNF4α enhanced PPARα/RXRα transactivation from TB PPRE3 in a DNA-binding independent manner. Coimmunoprecipitation assays supported the hypothesis that HNF4α was physically interacting with RXRα. RT-PCR performed with RNA from liver-specific HNF4α-null mice confirmed the involvement of HNF4α in the PPARα-regulated induction ofThbby Wy14,643. Overall, we conclude that HNF4α enhances the PPARα-mediated activation ofThbgene expression in part through interaction with the obligate PPARα partner, RXRα.

Published
2010
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4. Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters

Author

Olivier Bastien, Athel Cornish-Bowden, Renaud Dumas, Mylène Robert-Genthon, María Luz Cárdenas, Gilles Curien, Laboratoire de physiologie cellulaire végétale (LPCV), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Chloroplasts, Arabidopsis thaliana, Arabidopsis, Aspartate metabolism, plant, 01 natural sciences, enzyme kinetics, Threonine, Amino Acids, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, aspartate, Mathematical model, Applied Mathematics, simulation, Recombinant Proteins, Computational Theory and Mathematics, Biochemistry, General Agricultural and Biological Sciences, Biological system, aminoacid, flux distribution, Information Systems, metabolism, metabolic pathway, mathematical model, allosteric regulation, threonine, Allosteric regulation, Biology, Kinetic energy, Models, Biological, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, aspartate metabolism, SDV:BBM, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Computer Simulation, 030304 developmental biology, Aspartic Acid, General Immunology and Microbiology, Kinetic model, Reproducibility of Results, biology.organism_classification, Kinetics, Function (biology), 010606 plant biology & botany
Abstract

International audience; The aspartate-derived amino-acid pathway from plants is well suited for analysing the function of the allosteric network of interactions in branched pathways. For this purpose, a detailed kinetic model of the system in the plant model Arabidopsis was constructed on the basis of in vitro kinetic measurements. The data, assembled into a mathematical model, reproduce in vivo measurements and also provide non-intuitive predictions. A crucial result is the identification of allosteric interactions whose function is not to couple demand and supply but to maintain a high independence between fluxes in competing pathways. In addition, the model shows that enzyme isoforms are not functionally redundant, because they contribute unequally to the flux and its regulation. Another result is the identification of the threonine concentration as the most sensitive variable in the system, suggesting a regulatory role for threonine at a higher level of integration.

Published
2009
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