49 results on '"Rubtsova, Maria"'
Search Results
2. Encapsulation of octadecane through crosslinking of cellulose nanofibrils at the interface of Pickering emulsion: Effect of ionic strength on cellulose assembly and capsule shell properties
- Author
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Voronin, Denis V., Zaytseva, Nina E., Sitmukhanova, Eliza A., Bardina, Kristina A., Rubtsova, Maria I., Cherednichenko, Kirill A., Novikov, Andrei A., and Vinokurov, Vladimir A.
- Published
- 2024
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3. Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells
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Larionova, Tatyana D., Bastola, Soniya, Aksinina, Tatiana E., Anufrieva, Ksenia S., Wang, Jia, Shender, Victoria O., Andreev, Dmitriy E., Kovalenko, Tatiana F., Arapidi, Georgij P., Shnaider, Polina V., Kazakova, Anastasia N., Latyshev, Yaroslav A., Tatarskiy, Victor V., Shtil, Alexander A., Moreau, Pascale, Giraud, Francis, Li, Chaoxi, Wang, Yichan, Rubtsova, Maria P., Dontsova, Olga A., Condro, Michael, Ellingson, Benjamin M., Shakhparonov, Mikhail I., Kornblum, Harley I., Nakano, Ichiro, and Pavlyukov, Marat S.
- Published
- 2022
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4. Panel of potential lncRNA biomarkers can distinguish various types of liver malignant and benign tumors
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Burenina, Olga Y., Lazarevich, Natalia L., Kustova, Inna F., Shavochkina, Daria A., Moroz, Ekaterina A., Kudashkin, Nikolay E., Patyutko, Yuriy I., Metelin, Alexey V., Kim, Eduard F., Skvortsov, Dmitry A., Zatsepin, Timofei S., Rubtsova, Maria P., and Dontsova, Olga A.
- Published
- 2021
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5. New Fire-Retardant Open-Cell Composite Polyurethane Foams Based on Triphenyl Phosphate and Natural Nanoscale Additives.
- Author
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Cherednichenko, Kirill, Smirnov, Egor, Rubtsova, Maria, Repin, Dmitrii, and Semenov, Anton
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URETHANE foam ,HALLOYSITE ,FIREPROOFING agents ,FIRE testing ,FOAM ,ADDITIVES ,PHOSPHATES - Abstract
Despite the mechanical and physical properties of polyurethane foams (PUF), their application is still hindered by high inflammability. The elaboration of effective, low-cost, and environmentally friendly fire retardants remains a pressing issue that must be addressed. This work aims to show the feasibility of the successful application of natural nanomaterials, such as halloysite nanotubes and nanocellulose, as promising additives to the commercial halogen-free, fire-retardant triphenyl phosphate (TPP) to enhance the flame retardance of open-cell polyurethane foams. The nanocomposite foams were synthesized by in situ polymerization. Investigation of the mechanical properties of the nanocomposite PUF revealed that the nanoscale additives led to a notable decrease in the foam's compressibility. The obtained results of the flammability tests clearly indicate that there is a prominent synergetic effect between the fire-retardant and the natural nanoscale additives. The nanocomposite foams containing a mixture of TPP (10 and 20 parts per hundred polyol by weight) and either 10 wt.% of nanocellulose or 20 wt.% of halloysite demonstrated the lowest burning rate without dripping and were rated as HB materials according to UL 94 classification. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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6. Phenomenon for the modern ferromanganese mineralization in aeolian sediments along the coastal zone of Lake Baikal (Siberia)
- Author
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Akulov, Nikolay I, Rubtsova, Maria N, Akulova, Varvara V, and Shchetnikov, Alexander A
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- 2022
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7. LINC00116 codes for a mitochondrial peptide linking respiration and lipid metabolism
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Chugunova, Anastasia, Loseva, Elizaveta, Mazin, Pavel, Mitina, Aleksandra, Navalayeu, Tsimafei, Bilan, Dmitry, Vishnyakova, Polina, Marey, Maria, Golovina, Anna, Serebryakova, Marina, Pletnev, Philipp, Rubtsova, Maria, Mair, Waltraud, Vanyushkina, Anna, Khaitovich, Philipp, Belousov, Vsevolod, Vysokikhh, Mikhail, Sergiev, Petr, and Dontsova, Olga
- Published
- 2019
8. Human RPF1 and ESF1 in Pre-rRNA Processing and the Assembly of Pre-Ribosomal Particles: A Functional Study.
- Author
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Deryabin, Alexander, Moraleva, Anastasiia, Dobrochaeva, Kira, Kovaleva, Diana, Rubtsova, Maria, Dontsova, Olga, and Rubtsov, Yury
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ORGANELLE formation ,RIBOSOMAL proteins ,CYTOSKELETAL proteins ,RIBOSOMAL RNA ,CELL lines ,HUMAN beings - Abstract
Ribosome biogenesis is essential for the functioning of living cells. In higher eukaryotes, this multistep process is tightly controlled and involves a variety of specialized proteins and RNAs. This pool of so-called ribosome biogenesis factors includes diverse proteins with enzymatic and structural functions. Some of them have homologs in yeast S. cerevisiae, and their function can be inferred from the structural and biochemical data obtained for the yeast counterparts. The functions of human proteins RPF1 and ESF1 remain largely unclear, although RPF1 has been recently shown to participate in 60S biogenesis. Both proteins have drawn our attention since they contribute to the early stages of ribosome biogenesis, which are far less studied than the later stages. In this study, we employed the loss-of-function shRNA/siRNA-based approach to the human cell line HEK293 to determine the role of RPF1 and ESF1 in ribosome biogenesis. Downregulating RPF1 and ESF1 significantly changed the pattern of RNA products derived from 47S pre-rRNA. Our findings demonstrate that RPF1 and ESF1 are associated with different pre-ribosomal particles, pre-60S, and pre-40S particles, respectively. Our results allow for speculation about the particular steps of pre-rRNA processing, which highly rely on the RPF1 and ESF1 functions. We suggest that both factors are not directly involved in pre-rRNA cleavage but rather help pre-rRNA to acquire the conformation favoring its cleavage. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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9. Ultrahigh-throughput functional profiling of microbiota communities
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Terekhov, Stanislav S., Smirnov, Ivan V., Malakhova, Maja V., Samoilov, Andrei E., Manolov, Alexander I., Nazarov, Anton S., Danilov, Dmitry V., Dubiley, Svetlana A., Osterman, Ilya A., Rubtsova, Maria P., Kostryukova, Elena S., Ziganshin, Rustam H., Kornienko, Maria A., Vanyushkina, Anna A., Bukato, Olga N., Ilina, Elena N., Vlasov, Valentin V., Severinov, Konstantin V., Gabibov, Alexander G., and Altman, Sidney
- Published
- 2018
10. Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
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Terekhov, Stanislav S., Smirnov, Ivan V., Stepanova, Anastasiya V., Bobik, Tatyana V., Mokrushina, Yuliana A., Ponomarenko, Natalia A., Belogurov, Alexey A., Rubtsova, Maria P., Kartseva, Olga V., Gomzikova, Marina O., Moskovtsev, Alexey A., Bukatin, Anton S., Dubina, Michael V., Kostryukova, Elena S., Babenko, Vladislav V., Vakhitova, Maria T., Manolov, Alexander I., Malakhova, Maja V., Kornienko, Maria A., Tyakht, Alexander V., Vanyushkina, Anna A., Ilina, Elena N., Masson, Patrick, Gabibov, Alexander G., and Altman, Sidney
- Published
- 2017
11. Natural Fibrous Materials Based on Fungal Mycelium Hyphae as Porous Supports for Shape-Stable Phase-Change Composites.
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Sayfutdinova, Adeliya R., Cherednichenko, Kirill A., Rakitina, Maria A., Dubinich, Valeria N., Bardina, Kristina A., Rubtsova, Maria I., Petrova, Daria A., Vinokurov, Vladimir A., and Voronin, Denis V.
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MYCELIUM ,PARAFFIN wax ,PHASE change materials ,SOLID-state fermentation ,LATENT heat ,HEAT storage ,MORTAR - Abstract
Adsorption of organic phase-change materials (PCMs) by the porous matrix of microfibrillar cellulose (MFC) is a simple and versatile way to prepare shape-stable phase-change composites, which are promising as sustainable thermoregulating additives to construction materials. However, due to MFC inherent morphology, the resulting composites have relatively low poured density that complicates their introduction in sufficient amounts, for instance, into mortar mixes. Unlike MFC, fungal mycelium has, by an order, less fibrils thickness and, thus, possesses significantly higher poured density. Herein, we studied the feasibility of fungal mycelium-based matrices as alternative biopolymeric porous supports for preparation of sustainable and shape-stable phase-change composites. Two methods were employed to prepare the porous mycelium-based supports. The first one was the solid-state fermentation, which resulted in partial biotransformation of MFCs to mycelium hyphae, while the second one was the liquid-state surface fermentation, used to cultivate the reference matrix of Trametes hirsuta hyphae. The phase-change composites were prepared by adsorption of model organic PCMs on porous biopolymer matrices. The mass ratio of support/PCM was 40/60 wt%. The composites were studied with respect to their structure, composition, poured density, latent heat storage properties, and thermal and shape stability. The employment of the partially transformed to mycelium-hyphae MFC fibers was found to be a suitable way to prepare phase-change composites with improved poured density while preserving a reasonable latent heat capacity and shape stability as compared to the MFC/PCM composites. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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12. Hydroisomerization Catalysts for High-Quality Diesel Fuel Production.
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Aljajan, Yamen, Stytsenko, Valentin, Rubtsova, Maria, and Glotov, Aleksandr
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DIESEL fuels ,CATALYSTS ,CATALYSIS ,PETROLEUM chemicals industry ,CARBONACEOUS aerosols ,ISOMERIZATION - Abstract
Upgrading the properties of diesel fractions is considered one of the crucial processes in the petrochemical industry; and for this purpose in laboratory-scale researching it is studied on the base of the hydroisomerization of n-hexadecane as a main model reaction. Recently, zeolite-based bifunctional catalysts have proven their efficiency due to their remarkable acidity, shape-selectivity and relative resistance to deactivation. In this review, different topological-type zeolite-based catalysts, the mechanism of their catalytic effect in n-C
16 isomerization, and the principles of shape-selectivity are reviewed. A comparison of their structural-operational characteristics is made. The impact of some feedstock impurities on the catalyst's performance and deactivation due to carbonaceous deposits as well as various modern eco-friendly cost-effective synthesis techniques are also discussed. [ABSTRACT FROM AUTHOR]- Published
- 2023
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13. Polyurethane/ n -Octadecane Phase-Change Microcapsules via Emulsion Interfacial Polymerization: The Effect of Paraffin Loading on Capsule Shell Formation and Latent Heat Storage Properties.
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Voronin, Denis V., Sitmukhanova, Eliza, Mendgaziev, Rais I., Rubtsova, Maria I., Kopitsyn, Dmitry, Cherednichenko, Kirill A., Semenov, Anton P., Fakhrullin, Rawil, Shchukin, Dmitry G., and Vinokurov, Vladimir
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HEAT storage ,LATENT heat ,HEAT of formation ,EMULSION polymerization ,PHASE change materials ,FOOD emulsions ,POLYURETHANE elastomers - Abstract
Organic phase-change materials (PCMs) hold promise in developing advanced thermoregulation and responsive energy systems owing to their high latent heat capacity and thermal reliability. However, organic PCMs are prone to leakages in the liquid state and, thus, are hardly applicable in their pristine form. Herein, we encapsulated organic PCM n-Octadecane into polyurethane capsules via polymerization of commercially available polymethylene polyphenylene isocyanate and polyethylene glycol at the interface oil-in-water emulsion and studied how various n-Octadecane feeding affected the shell formation, capsule structure, and latent heat storage properties. The successful shell polymerization and encapsulation of n-Octadecane dissolved in the oil core was verified by confocal microscopy and Fourier-transform infrared spectroscopy. The mean capsule size varied from 9.4 to 16.7 µm while the shell was found to reduce in thickness from 460 to 220 nm as the n-Octadecane feeding increased. Conversely, the latent heat storage capacity increased from 50 to 132 J/g corresponding to the growth in actual n-Octadecane content from 25% to 67% as revealed by differential scanning calorimetry. The actual n-Octadecane content increased non-linearly along with the n-Octadecane feeding and reached a plateau at 66–67% corresponded to 3.44–3.69 core-to-monomer ratio. Finally, the capsules with the reasonable combination of structural and thermal properties were evaluated as a thermoregulating additive to a commercially available paint. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
14. Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay
- Author
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Kalinina, Marina A., Skvortsov, Dmitry A., Rubtsova, Maria P., Komarova, Ekaterina S., and Dontsova, Olga A.
- Published
- 2018
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15. Human nucleolar protein SURF6/RRP14 participates in early steps of pre-rRNA processing.
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Moraleva, Anastasiia, Deryabin, Alexander, Kordyukova, Maria, Polzikov, Mikhail, Shishova, Kseniya, Dobrochaeva, Kira, Rubtsov, Yury, Rubtsova, Maria, Dontsova, Olga, and Zatsepina, Olga
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NUCLEAR proteins ,RIBOSOMAL proteins ,MOLECULAR chaperones ,HELA cells ,CELL cycle ,RIBOSOMAL RNA - Abstract
The biogenesis of ribosomes requires tightly controlled transcription and processing of pre-rRNA which comprises ribosomal RNAs forming the core of large and small ribosomal subunits. Early steps of the pre-rRNA processing and assembly of the ribosomal subunits require a large set of proteins that perform folding and nucleolytic cleavage of pre-rRNAs in the nucleoli. Structure and functions of proteins involved in the pre-rRNA processing have been extensively studied in the budding yeast S. cerevisiae. Functional characterization of their human homologues is complicated by the complexity of mammalian ribosomes and increased number of protein factors involved in the ribosomal biogenesis. Homologues of human nucleolar protein SURF6 from yeast and mouse, Rrp14 and Surf6, respectively, had been shown to be involved in the early steps of pre-rRNA processing. Rrp14 works as RNA chaperone in complex with proteins Ssf1 and Rrp15. Human SURF6 knockdown and overexpression were used to clarify a role of SURF6 in the early steps of pre-rRNA processing in human cell lines HeLa and HTC116. By analyzing the abundance of the rRNA precursors in cells with decreased level or overexpression of SURF6, we demonstrated that human SURF6 is involved in the maturation of rRNAs from both small and large ribosomal subunits. Changes in the SURF6 level caused by knockdown or overexpression of the protein do not result in the death of HeLa cells in contrast to murine embryonic fibroblasts, but significantly alter the distribution of cells among the phases of the cell cycle. SURF6 knockdown in both p53 sufficient and p53 deficient HCT116 human cancer cells results in elongation of G0/G1 and shortening of G2/M phase. This surprising result suggests p53 independence of SURF6 effects on the cell cycle and possible multiple functions of SURF6. Our data point to the shift from pathway 1 to pathway 2 of the rRNA biogenesis caused by the SURF6 knockdown and its likely association with p53 pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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16. Post-Transcriptional and Post-Translational Modifications in Telomerase Biogenesis and Recruitment to Telomeres.
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Shepelev, Nikita, Dontsova, Olga, and Rubtsova, Maria
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TELOMERES ,TELOMERASE ,POST-translational modification ,CELLULAR aging ,EMBRYOLOGY ,GERM cells ,STEM cells ,CANCER invasiveness - Abstract
Telomere length is associated with the proliferative potential of cells. Telomerase is an enzyme that elongates telomeres throughout the entire lifespan of an organism in stem cells, germ cells, and cells of constantly renewed tissues. It is activated during cellular division, including regeneration and immune responses. The biogenesis of telomerase components and their assembly and functional localization to the telomere is a complex system regulated at multiple levels, where each step must be tuned to the cellular requirements. Any defect in the function or localization of the components of the telomerase biogenesis and functional system will affect the maintenance of telomere length, which is critical to the processes of regeneration, immune response, embryonic development, and cancer progression. An understanding of the regulatory mechanisms of telomerase biogenesis and activity is necessary for the development of approaches toward manipulating telomerase to influence these processes. The present review focuses on the molecular mechanisms involved in the major steps of telomerase regulation and the role of post-transcriptional and post-translational modifications in telomerase biogenesis and function in yeast and vertebrates. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
17. Phase-Change Microcapsules with a Stable Polyurethane Shell through the Direct Crosslinking of Cellulose Nanocrystals with Polyisocyanate at the Oil/Water Interface of Pickering Emulsion.
- Author
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Voronin, Denis, Mendgaziev, Rais, Sayfutdinova, Adeliya, Kugai, Maria, Rubtsova, Maria, Cherednichenko, Kirill, Shchukin, Dmitry, and Vinokurov, Vladimir
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CELLULOSE nanocrystals ,REVERSIBLE phase transitions ,HEAT storage ,PHASE change materials ,LATENT heat ,POLYURETHANES ,POLYOLS - Abstract
Phase-change materials (PCMs) attract much attention with regard to their capability of mitigating fossil fuel-based heating in in-building applications, due to the responsive accumulation and release of thermal energy as a latent heat of reversible phase transitions. Organic PCMs possess high latent heat storage capacity and thermal reliability. However, bare PCMs suffer from leakages in the liquid form. Here, we demonstrate a reliable approach to improve the shape stability of organic PCM n-octadecane by encapsulation via interfacial polymerization at an oil/water interface of Pickering emulsion. Cellulose nanocrystals are employed as emulsion stabilizers and branched oligo-polyol with high functionality to crosslink the polyurethane shell in reaction with polyisocyanate dissolved in the oil core. This gives rise to a rigid polyurethane structure with a high density of urethane groups. The formation of a polyurethane shell and successful encapsulation of n-octadecane is confirmed by FTIR spectroscopy, XRD analysis, and fluorescent confocal microscopy. Electron microscopy reveals the formation of non-aggregated capsules with an average size of 18.6 µm and a smooth uniform shell with the thickness of 450 nm. The capsules demonstrate a latent heat storage capacity of 79 J/g, while the encapsulation of n-octadecane greatly improves its shape and thermal stability compared with bulk paraffin. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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18. Natural aluminosilicate nanotubes loaded with RuCo as nanoreactors for Fischer-Tropsch synthesis.
- Author
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Mazurova, Kristina, Glotov, Aleksandr, Kotelev, Mikhail, Eliseev, Oleg, Gushchin, Pavel, Rubtsova, Maria, Vutolkina, Anna, Kazantsev, Ruslan, Vinokurov, Vladimir, and Stavitskaya, Anna
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HALLOYSITE ,RUTHENIUM catalysts ,FISCHER-Tropsch process ,POROUS materials ,NANOTUBES ,RUTHENIUM ,REDUCING agents ,COBALT - Abstract
Following nanoarchitectural approach, mesoporous halloysite nanotubes with internal surface composed of alumina were loaded with 5-6 nm RuCo nanoparticles by sequential loading/ reduction procedure. Ruthenium nanoclusters were loaded inside clay tube by microwaveassisted method followed by cobalt ions electrostatic attraction to ruthenium during wetness impregnation step. Developed nanoreactors with bimetallic RuCo nanoparticles were investigated as catalysts for the Fischer-Tropsch process. The catalyst with 14.3 wt.% of Co and 0.15 wt.% of Ru showed high activity (CO conversion reached 24.6%), low selectivity to methane (11.9%), CO
2 (0.3%), selectivity to C5+ hydrocarbons of 79.1% and chain growth index (α) = 0.853. Proposed nanoreactors showed better selectivity to target products combined with high activity in comparison to the similar bimetallic systems supported on synthetic porous materials. It was shown that reducing agent (NaBH4 or H2) used to obtain Ru nanoclusters at first synthesis step played a very important role in the reducibility and selectivity of resulting RuCo catalysts. [ABSTRACT FROM AUTHOR]- Published
- 2022
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19. Flow-Seq Evaluation of Translation Driven by a Set of Natural Escherichia coli 5′-UTR of Variable Length.
- Author
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Komarova, Ekaterina S., Slesarchuk, Anna N., Rubtsova, Maria P., Osterman, Ilya A., Tupikin, Alexey E., Pyshnyi, Dmitry V., Dontsova, Olga A., Kabilov, Marsel R., and Sergiev, Petr V.
- Subjects
ESCHERICHIA coli ,NUCLEOTIDE sequencing - Abstract
Flow-seq is a method that combines fluorescently activated cell sorting and next-generation sequencing to deduce a large amount of data about translation efficiency from a single experiment. Here, we constructed a library of fluorescent protein-based reporters preceded by a set of 648 natural 5′-untranslated regions (5′-UTRs) of Escherichia coli genes. Usually, Flow-seq libraries are constructed using uniform-length sequence elements, in contrast to natural situations, where functional elements are of heterogenous lengths. Here, we demonstrated that a 5′-UTR library of variable length could be created and analyzed with Flow-seq. In line with previous Flow-seq experiments with randomized 5′-UTRs, we observed the influence of an RNA secondary structure and Shine–Dalgarno sequences on translation efficiency; however, the variability of these parameters for natural 5′-UTRs in our library was smaller in comparison with randomized libraries. In line with this, we only observed a 30-fold difference in translation efficiency between the best and worst bins sorted with this factor. The results correlated with those obtained with ribosome profiling. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
20. Architectural design of core-shell nanotube systems based on aluminosilicate clay.
- Author
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Stavitskaya, Anna, Rubtsova, Maria, Glotov, Aleksandr, Vinokurov, Vladimir, Vutolkina, Anna, Fakhrullin, Rawil, and Lvov, Yuri
- Published
- 2022
- Full Text
- View/download PDF
21. How Structural Features Define Biogenesis and Function of Human Telomerase RNA Primary Transcript.
- Author
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Rubtsova, Maria and Dontsova, Olga
- Subjects
TELOMERASE ,REVERSE transcriptase ,RNA ,CELL proliferation ,EUKARYOTIC cells ,AUTOPHAGY - Abstract
Telomerase RNA has been uncovered as a component of the telomerase enzyme, which acts as a reverse transcriptase and maintains the length of telomeres in proliferated eukaryotic cells. Telomerase RNA is considered to have major functions as a template for telomeric repeat synthesis and as a structural scaffold for telomerase. However, investigations of its biogenesis and turnover, as well as structural data, have provided evidence of functions of telomerase RNA that are not associated with telomerase activity. The primary transcript produced from the human telomerase RNA gene encodes for the hTERP protein, which presents regulatory functions related to autophagy, cellular proliferation, and metabolism. This review focuses on the specific features relating to the biogenesis and structure of human telomerase RNA that support the existence of an isoform suitable for functioning as an mRNA. We believe that further investigation into human telomerase RNA biogenesis mechanisms will provide more levels for manipulating cellular homeostasis, survival, and transformation mechanisms, and may contribute to a deeper understanding of the mechanisms of aging. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. Chimeric bifunctional oligonucleotides as a novel tool to invade telomerase assembly
- Author
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Azhibek, Dulat, Zvereva, Maria, Zatsepin, Timofei, Rubtsova, Maria, and Dontsova, Olga
- Published
- 2014
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23. Role of RNA Biogenesis Factors in the Processing and Transport of Human Telomerase RNA.
- Author
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Pakhomova, Tatiana, Moshareva, Maria, Vasilkova, Daria, Zatsepin, Timofey, Dontsova, Olga, and Rubtsova, Maria
- Subjects
TELOMERASE ,NON-coding RNA ,RNA ,TRANSCRIPTION factors ,GENE expression - Abstract
Telomerase RNA has long been considered to be a noncoding component of telomerase. However, the expression of the telomerase RNA gene is not always associated with telomerase activity. The existence of distinct TERC gene expression products possessing different functions were demonstrated recently. During biogenesis, hTR is processed by distinct pathways and localized in different cell compartments, depending on whether it functions as a telomerase complex component or facilitates antistress activities as a noncoding RNA, in which case it is either processed in the mitochondria or translated. In order to identify the factors responsible for the appearance and localization of the exact isoform of hTR, we investigated the roles of the factors regulating transcription DSIF (Spt5) and NELF-E; exosome-attracting factors ZCCHC7, ZCCHC8, and ZFC3H1; ARS2, which attracts processing and transport factors; and transport factor PHAX during the biogenesis of hTR. The data obtained revealed that ZFC3H1 participates in hTR biogenesis via pathways related to the polyadenylated RNA degradation mechanism. The data revealed essential differences that are important for understanding hTR biogenesis and that are interesting for further investigations of new, therapeutically significant targets. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
24. Little but Loud. The Diversity of Functions of Small Proteins and Peptides – Translational Products of Short Reading Frames.
- Author
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Sergiev, Petr V. and Rubtsova, Maria P.
- Subjects
- *
FRAMES (Social sciences) , *PEPTIDES , *OPEN reading frames (Genetics) , *FUNCTIONAL genomics , *PROTEINS , *CELLULAR control mechanisms - Abstract
Cell functioning is tightly regulated process. For many years, research in the fields of proteomics and functional genomics has been focused on the role of proteins in cell functioning. The advances in science have led to the uncovering that short open reading frames, previously considered non-functional, serve a variety of functions. Short reading frames in polycistronic mRNAs often regulate their stability and translational efficiency of the main reading frame. The improvement of proteomic analysis methods has made it possible to identify the products of translation of short open reading frames in quantities that suggest the existence of functional role of those peptides and short proteins. Studies demonstrating their role unravel a new level of the regulation of cell functioning and its adaptation to changing conditions. This review is devoted to the analysis of functions of recently discovered peptides and short proteins. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
25. Rewiring of growth-dependent transcription regulation by a point mutation in region 1.1 of the housekeeping σ factor.
- Author
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Pletnev, Philipp, Pupov, Danil, Pshanichnaya, Lizaveta, Esyunina, Daria, Petushkov, Ivan, Nesterchuk, Mikhail, Osterman, Ilya, Rubtsova, Maria, Mardanov, Andrey, Ravin, Nikolai, Sergiev, Petr, Kulbachinskiy, Andrey, and Dontsova, Olga
- Published
- 2020
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26. METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue.
- Author
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Laptev, Ivan, Shvetsova, Ekaterina, Levitskii, Sergey, Serebryakova, Marina, Rubtsova, Maria, Zgoda, Victor, Bogdanov, Alexey, Kamenski, Piotr, Sergiev, Petr, and Dontsova, Olga
- Published
- 2020
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- View/download PDF
27. Translation at first sight: the influence of leading codons.
- Author
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Osterman, Ilya A, Chervontseva, Zoe S, Evfratov, Sergey A, Sorokina, Alena V, Rodin, Vladimir A, Rubtsova, Maria P, Komarova, Ekaterina S, Zatsepin, Timofei S, Kabilov, Marsel R, Bogdanov, Alexey A, Gelfand, Mikhail S, Dontsova, Olga A, and Sergiev, Petr V
- Published
- 2020
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- View/download PDF
28. Influence of the spacer region between the Shine–Dalgarno box and the start codon for fine‐tuning of the translation efficiency in Escherichia coli.
- Author
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Komarova, Ekaterina S., Chervontseva, Zoya S., Osterman, Ilya A., Evfratov, Sergey A., Rubtsova, Maria P., Zatsepin, Timofei S., Semashko, Tatiana A., Kostryukova, Elena S., Bogdanov, Alexey A., Gelfand, Mikhail S., Dontsova, Olga A., and Sergiev, Petr V.
- Subjects
TRANSLATIONS ,NUCLEOTIDE sequencing ,GENE expression ,BACTERIAL genes ,SEQUENCE analysis ,ESCHERICHIA coli - Abstract
Summary: Translation efficiency contributes several orders of magnitude difference in the overall yield of exogenous gene expression in bacteria. In diverse bacteria, the translation initiation site, whose sequence is the primary determinant of the translation performance, is comprised of the start codon and the Shine–Dalgarno box located upstream. Here, we have examined how the sequence of a spacer between these main components of the translation initiation site contributes to the yield of synthesized protein. We have created a library of reporter constructs with the randomized spacer region, performed fluorescently activated cell sorting and applied next‐generation sequencing analysis (the FlowSeq protocol). As a result, we have identified sequence motifs for the spacer region between the Shine–Dalgarno box and AUG start codon that may modulate the translation efficiency in a 100‐fold range. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
29. Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m5U formation in both tRNA and rRNA.
- Author
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Laptev, Ivan, Shvetsova, Ekaterina, Levitskii, Sergey, Serebryakova, Marina, Rubtsova, Maria, Bogdanov, Alexey, Kamenski, Piotr, Sergiev, Petr, and Dontsova, Olga
- Abstract
RNA molecules of all species contain modified nucleotides and particularly m
5 U residues. The vertebrate mitochondrial small subunit rRNA contains m5 U nucleotide in a unique site. In this work we found an enzyme, TRMT2B, responsible for the formation of this nucleotide and m5 U residues in a number of mitochondrial tRNA species. Inactivation of the Trmt2B gene leads to a reduction of the activity of respiratory chain complexes I, III and IV, containing the subunits synthesized by the mitochondrial protein synthesis apparatus. Comparative sequence analysis of m5 U-specific RNA methyltransferases revealed an unusual evolutionary pathway of TRMT2B formation which includes consecutive substrate specificity switches from the large subunit rRNA to tRNA and then to the small subunit rRNA. [ABSTRACT FROM AUTHOR]- Published
- 2020
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30. Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases.
- Author
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Pletnev, Philipp, Guseva, Ekaterina, Zanina, Anna, Evfratov, Sergey, Dzama, Margarita, Treshin, Vsevolod, Pogorel'skaya, Alexandra, Osterman, Ilya, Golovina, Anna, Rubtsova, Maria, Serebryakova, Marina, Pobeguts, Olga V., Govorun, Vadim M., Bogdanov, Alexey A., Dontsova, Olga A., and Sergiev, Petr V.
- Subjects
RIBOSOMAL RNA ,ESCHERICHIA coli ,FUNCTIONAL analysis ,RIBOSOMES ,METHYLTRANSFERASES ,REPORTER genes ,RIBOSOMAL proteins - Abstract
Ribosomal RNAs in all organisms are methylated. The functional role of the majority of modified nucleotides is unknown. We systematically questioned the influence of rRNA methylation in Escherichia coli on a number of characteristics of bacterial cells with the help of a set of rRNA methyltransferase (MT) gene knockout strains from the Keio collection. Analysis of ribosomal subunits sedimentation profiles of the knockout strains revealed a surprisingly small number of rRNA MT that significantly affected ribosome assembly. Accumulation of the assembly intermediates was observed only for the rlmE knockout strain whose growth was retarded most significantly among other rRNA MT knockout strains. Accumulation of the 17S rRNA precursor was observed for rsmA (ksgA) knockout cells as well as for cells devoid of functional rsmB and rlmC genes. Significant differences were found among the WT and the majority of rRNA MT knockout strains in their ability to sustain exogenous protein overexpression. While the majority of the rRNA MT knockout strains supported suboptimal reporter gene expression, the strain devoid of the rsmF gene demonstrated a moderate increase in the yield of ectopic gene expression. Comparative 2D protein gel analysis of rRNA MT knockout strains revealed only minor perturbations of the proteome. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
31. Protein encoded in human telomerase RNA is involved in cell protective pathways.
- Author
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Rubtsova, Maria, Naraykina, Yulia, Vasilkova, Daria, Meerson, Mark, Zvereva, Maria, Prassolov, Vladimir, Lazarev, Vasily, Manuvera, Valentin, Kovalchuk, Sergey, and Anikanov, Nickolay
- Published
- 2018
- Full Text
- View/download PDF
32. Corpus-based conceptualization in sociology: possibilities and limits.
- Author
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RUBTSOVA, MARIA, VASILIEVA, ELENA, PAVENKOV, OLEG, and PAVENKOV, VLADIMIR
- Subjects
- *
SOCIOLOGY , *SOCIOLOGICAL research , *ALTRUISM , *CORPORA , *SOCIAL problems - Abstract
The problem of the quantitative interpretation of qualitative data is one of the most important in sociological research. Textual analysis has placed emphasis on deep and careful study of texts how personal strategies embodied in the concepts. However, quantitative interpretation has always been problematic. Our paper deals with the corpus-based conceptualization method, which can considered as a method of collecting and organizing data material from linguistic corpora. The corpus-based conceptualization allows us to establish a closer link with the meaning and identify the whole spectrum of meanings. It shows that some sociologists lose essential meanings in the research process because of lack of in-deep immersion in the daily life and speech of the people. We chose the concepts of "altruism" and "mercy" as examples to demonstrate the corpus-based conceptualization and its place in sociological research methodology. Data comes from the Russian National Corpus. The Russian National Corpus consists of 1802 relevant words, with 775 for altruism and 1047 for mercy. Data processing carried out by SPSS 19.0. As the result, we have discussed what difficulties the researcher can meet using this method and have offered SFL and Role and Reference grammar as a way to accurately determine the context. Our suggestions can be used in the preparation of questionnaires, guides, in an analysis of interview transcripts. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
33. The transformation of gender visualization in photography: Soviet and Russian multisemiotics.
- Author
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Pavenkov, Oleg, Rubtsova, Maria, and Shmelev, Ilya
- Subjects
- *
GENDER identity , *GENDER inequality - Abstract
The article is devoted to the analysis of the construction of new system of gender images in the sociopolitical conditions in Russia during the last thirty ages. Based on Kress and Van Leeuwen multisemiotic theory and the ideas A. Dudareva, I.Groshev and M. Petrova, we conducted a research of gender images of women in the Soviet Union and modern Russian advertising. The data comes from 300 images of the Russian men and women in advertising. These 300 images were specifically chosen by the author in order to be acceptable for coding. As the result of the study, we developed the typology of gender images of woman in Russian advertisement. Our conclusion was that economic, political and socio-cultural factors were the main factors in the transformation of gender images in modern Russia. The gender of a woman, her body and figures as shown in the media, very often were object of sexual exploitation, when the impact of using the image and play with human sexual passion forces her to commit certain actions, such as buying goods. This characteristic of our commercialization of gender images turns a person, both men and women, into a commodity. Gender is becoming the object that can be purchased and can be enjoyed. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
34. Interstitial telomeric repeats-associated DNA breaks.
- Author
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Shubernetskaya, Olga, Skvortsov, Dmitry, Evfratov, Sergey, Rubtsova, Maria, Belova, Elena, Strelkova, Olga, Cherepaninets, Varvara, Zhironkina, Oxana, Olovnikov, Alexey, Zvereva, Maria, Dontsova, Olga, and Kireev, Igor
- Subjects
DOUBLE-strand DNA breaks ,GENE amplification ,DNA damage ,EUKARYOTIC genomes ,SINGLE-strand DNA breaks ,POLYMERASE chain reaction - Abstract
During a cell's lifespan, DNA break formation is a common event, associated with many processes, from replication to apoptosis. Most of DNA breaks are readily repaired, but some are meant to persist in time, such as the chromosome ends, protected by telomeres. Besides them, eukaryotic genomes comprise shorter stretches of interstitial telomeric repeats. We assumed that the latter may also be associated with the formation of DNA breaks meant to persist in time. In zebrafish and mouse embryos, cells containing numerous breakage foci were identified. These breaks were not associated with apoptosis or replication, nor did they seem to activate DNA damage response machinery. Unlike short-living, accidental sparse breaks, the ones we found seem to be closely associated, forming discrete break foci. A PCR-based method was developed, allowing specific amplification of DNA regions located between inverted telomeric repeats associated with breaks. The cloning and sequencing of such DNA fragments were found to denote some specificity in their distribution for different tissue types and development stages. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
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- View/download PDF
35. La medición de la cohesión del personal a través de la investigación multimodal en organizaciones de gestión pública y privada.
- Author
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Andreeva, Maria and Rubtsova, Maria V.
- Abstract
Copyright of Dilemas Contemporáneos: Educación, Política y Valores is the property of Dilemas Contemporaneos: Educacion, Politica y Valores and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
36. The genome-wide transcription response to telomerase deficiency in the thermotolerant yeast Hansenula polymorpha DL-1.
- Author
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Beletsky, Alexey V., Malyavko, Alexander N., Sukhanova, Maria V., Mardanova, Eugenia S., Zvereva, Maria I., Petrova, Olga A., Parfenova, Yulia Yu., Rubtsova, Maria P., Mardanov, Andrey V., Lavrik, Olga I., Dontsova, Olga A., and Ravin, Nikolai V.
- Subjects
TELOMERASE ,YEAST fungi genetics ,EUKARYOTIC genomes ,CHROMOSOME replication ,NUCLEOPROTEINS - Abstract
Background: In the course of replication of eukaryotic chromosomes, the telomere length is maintained due to activity of telomerase, the ribonucleoprotein reverse transcriptase. Abolishing telomerase function causes progressive shortening of telomeres and, ultimately, cell cycle arrest and replicative senescence. To better understand the cellular response to telomerase deficiency, we performed a transcriptomic study for the thermotolerant methylotrophic yeast Hansenula polymorpha DL-1 lacking telomerase activity. Results: Mutant strain of H. polymorpha carrying a disrupted telomerase RNA gene was produced, grown to senescence and analyzed by RNA-seq along with wild type strain. Telomere shortening induced a transcriptional response involving genes relevant to telomere structure and maintenance, DNA damage response, information processing, and some metabolic pathways. Genes involved in DNA replication and repair, response to environmental stresses and intracellular traffic were up-regulated in senescent H. polymorpha cells, while strong down-regulation was observed for genes involved in transcription and translation, as well as core histones. Conclusions: Comparison of the telomerase deletion transcription responses by Saccharomyces cerevisiae and H. polymorpha demonstrates that senescence makes different impact on the main metabolic pathways of these yeast species but induces similar changes in processes related to nucleic acids metabolism and protein synthesis. Up-regulation of a subunit of the TORC1 complex is clearly relevant for both types of yeast. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
37. Application of sorting and next generation sequencing to study 5'-UTR influence on translation efficiency in Escherichia coli.
- Author
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Evfratov, Sergey A., Osterman, Ilya A., Komarova, Ekaterina S., Pogorelskaya, Alexandra M., Rubtsova, Maria P., Zatsepin, Timofei S., Semashko, Tatiana A., Kostryukova, Elena S., Mironov, Andrey A., Burnaev, Evgeny, Krymova, Ekaterina, Gelfand, Mikhail S., Govorun, Vadim M., Bogdanov, Alexey A., Sergiev, Petr V., and Dontsova, Olga A.
- Published
- 2017
- Full Text
- View/download PDF
38. Phase-change composites for bimodal solar/electromagnetic energy storage based on magnetite-modified cellulose microfibers.
- Author
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Pirtsul, Alexandra E., Rubtsova, Maria I., Mendgaziev, Rais I., Cherednichenko, Kirill A., Kruglov, Vladimir V., Komlev, Aleksei S., Lomova, Maria V., Vinokurov, Vladimir, and Voronin, Denis V.
- Subjects
- *
MICROFIBERS , *IRON oxide nanoparticles , *ELECTROMAGNETIC waves , *IRON oxides , *ENERGY storage , *CELLULOSE - Abstract
• Cellulose microfibers were modified with Fe 3 O 4 nanoparticles in a tunable way. • Adsorption of lauric acid onto Fe 3 O 4 -modified fibers leads to shape-stable PCM. • Magnetic PCM composites can store thermal energy under alternating magnetic field. • Magnetite nanoparticles improves light-to-thermal conversion of PCM composites. Fe 3 O 4 -modified cellulose microfibers containing 7, 16, and 31 wt% of magnetite were prepared via co-precipitation of Fe2+ and Fe3+ salts. The magnetic phase-change composites were synthesized by adsorption of lauric acid onto the modified fibers with the highest saturation magnetization (23 emu/g) and magnetic-to-thermal conversion. The resulted composites demonstrated the saturation magnetization of 11.2 emu/g and latent heat storage capacity of 90 J/g corresponding to the loading efficiency of lauric acid of 49–51 wt%. The IR-imaging revealed the efficient accumulation of latent heat in the phase-change composite under the simulated sunlight and high frequency alternating magnetic field along with the excellent shape stability of the composites during the melting of lauric acid. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
39. Mixed Valence Copper(I,II) Binuclear Complexes withUnexpected Structure: Synthesis, Biological Properties and AnticancerActivity.
- Author
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Majouga, Alexander G., Zvereva, Maria I., Rubtsova, Maria P., Skvortsov, Dmitry A., Mironov, Andrei V., Azhibek, Dulat M., Krasnovskaya, Olga O., Gerasimov, Vasily M., Udina, Anna V., Vorozhtsov, Nikolay I., Beloglazkina, Elena K., Agron, Leonid, Mikhina, Larisa V., Tretyakova, Alla V., Zyk, Nikolay V., Zefirov, Nikolay S., Kabanov, Alexander V., and Dontsova, Olga A.
- Published
- 2014
- Full Text
- View/download PDF
40. Deep Functional Profiling of Wild Animal Microbiomes Reveals Probiotic Bacillus pumilus Strains with a Common Biosynthetic Fingerprint.
- Author
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Baranova, Margarita N., Kudzhaev, Arsen M., Mokrushina, Yuliana A., Babenko, Vladislav V., Kornienko, Maria A., Malakhova, Maja V., Yudin, Victor G., Rubtsova, Maria P., Zalevsky, Arthur, Belozerova, Olga A., Kovalchuk, Sergey, Zhuravlev, Yuriy N., Ilina, Elena N., Gabibov, Alexander G., Smirnov, Ivan V., and Terekhov, Stanislav S.
- Subjects
BACILLUS pumilus ,RACCOON ,PROBIOTICS ,RACCOON dog ,HUMAN microbiota ,BROWN bear - Abstract
The biodiversity of microorganisms is maintained by intricate nets of interactions between competing species. Impaired functionality of human microbiomes correlates with their reduced biodiversity originating from aseptic environmental conditions and antibiotic use. Microbiomes of wild animals are free of these selective pressures. Microbiota provides a protecting shield from invasion by pathogens in the wild, outcompeting their growth in specific ecological niches. We applied ultrahigh-throughput microfluidic technologies for functional profiling of microbiomes of wild animals, including the skin beetle, Siberian lynx, common raccoon dog, and East Siberian brown bear. Single-cell screening of the most efficient killers of the common human pathogen Staphylococcus aureus resulted in repeated isolation of Bacillus pumilus strains. While isolated strains had different phenotypes, all of them displayed a similar set of biosynthetic gene clusters (BGCs) encoding antibiotic amicoumacin, siderophore bacillibactin, and putative analogs of antimicrobials including bacilysin, surfactin, desferrioxamine, and class IId cyclical bacteriocin. Amicoumacin A (Ami) was identified as a major antibacterial metabolite of these strains mediating their antagonistic activity. Genome mining indicates that Ami BGCs with this architecture subdivide into three distinct families, characteristic of the B. pumilus, B. subtilis, and Paenibacillus species. While Ami itself displays mediocre activity against the majority of Gram-negative bacteria, isolated B. pumilus strains efficiently inhibit the growth of both Gram-positive S. aureus and Gram-negative E. coli in coculture. We believe that the expanded antagonistic activity spectrum of Ami-producing B. pumilus can be attributed to the metabolomic profile predetermined by their biosynthetic fingerprint. Ultrahigh-throughput isolation of natural probiotic strains from wild animal microbiomes, as well as their metabolic reprogramming, opens up a new avenue for pathogen control and microbiome remodeling in the food industry, agriculture, and healthcare. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
41. Nanoarchitectural approach for synthesis of highly crystalline zeolites with a low Si/Al ratio from natural clay nanotubes.
- Author
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Rubtsova, Maria, Smirnova, Ekaterina, Boev, Sevastyan, Kotelev, Michail, Cherednichenko, Kirill, Vinokurov, Vladimir, Lvov, Yuri, and Glotov, Aleksandr
- Subjects
- *
CLAY , *ZEOLITES , *NANOTUBES , *HALLOYSITE , *MORDENITE , *SODALITE - Abstract
A nanoarchitectural approach for synthesis of highly crystalline zeolites based on natural aluminosilicate nanotubes with adjustable silica to alumina ratio has been developed. A double template technique was proposed for synthesis of zeolite microcrystals with geometric morphology defined by the initial aluminosilicate nanotubes (halloysite). Remarkable and unique zeolite microcrystal shapes (submicron spheres, pompon-like systems and coffin-shaped prisms) possessing different internal structures (sodalite, analcime, mordenite, mordenite framework inverted) were obtained from halloysite by hydrothermal method. Halloysite Si/Al adjustments (acid etching, addition of extra silica or alumina) significantly affected crystalline structure and morphology of the obtained materials, while changing organic templates did not have a considerable effect. [Display omitted] • A novel approach for zeolites synthesis from aluminosilicate nanotubes was proposed. • Zeolites morphology is defined by the initial aluminosilicate nanotubes. • SOD, ANA, MOR, and MFI microstructures were synthesized from natural nanoclay. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
42. CuO-In 2 O 3 Catalysts Supported on Halloysite Nanotubes for CO 2 Hydrogenation to Dimethyl Ether.
- Author
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Pechenkin, Alexey, Potemkin, Dmitry, Rubtsova, Maria, Snytnikov, Pavel, Plyusnin, Pavel, and Glotov, Aleksandr
- Subjects
CATALYST supports ,METHYL ether ,CARBON dioxide ,HALLOYSITE ,NANOTUBES ,HYDROGENATION - Abstract
Hydrogenation of CO
2 relative to valuable chemical compounds such as methanol or dimethyl ether (DME) is an attractive route for reducing CO2 emissions in the atmosphere. In the present work, the hydrogenation of CO2 into DME over CuO-In2 O3 , supported on halloysite nanotubes (HNT) was investigated in the temperature range 200–300 °C at 40 atm. HNT appears to be novel promising support for bifunctional catalysts due to its thermal stability and the presence of acidic sites on its surface. CuO-In2 O3 /HNT catalysts demonstrate higher CO2 conversion and DME selectivity compared to non-indium CuO/HNT catalysts. The catalysts were investigated by N2 adsorption, X-ray diffraction, hydrogen-temperature programmed reduction and transition electron microscopy. The acid sites were analyzed by temperature programmed desorption of ammonia. It was shown that CuO/HNT was unstable under reaction conditions in contrast to CuO-In2 O3 /HNT. The best CuO-In2 O3 /HNT catalyst provided CO2 conversion of 7.6% with 65% DME selectivity under P = 40 atm, T = 250 °C, gas hour space velocity 12,000 h−1 and H2 :CO2 = 3:1. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
43. Distinctive Properties of the 5'-Untranslated Region of Human Hsp70 mRNA.
- Author
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Rubtsova, Maria P., Sizova, Daria V., Dmitriev, Sergei E., Ivanov, Dmitri S., Prassolov, Vladimir S., and Shatsky, Ivan N.
- Subjects
- *
MESSENGER RNA , *HEAT shock proteins , *RIBOSOMES - Abstract
Demonstrates that the 5'-untranslated region (UTR) of human heat shock protein Hsp70 mRNA represents an internal ribosome entry site (IRES) with the relative activity similar to that of the classical picornaviral IRES. Expression of a downstream reporter gene directed by the 5'-UTR of Hsp70 mRNA in a dicistronic vector; Variance in the effect of small deletions within the 5'-UTR of Hsp70 mRNA on the IRES activity.
- Published
- 2003
- Full Text
- View/download PDF
44. Conversion of 48S translation preinitiation complexes into 80S initiation complexes as revealed by toeprinting
- Author
-
Dmitriev, Sergey E., Pisarev, Andrey V., Rubtsova, Maria P., Dunaevsky, Yan E., and Shatsky, Ivan N.
- Subjects
RETICULOCYTES ,RIBOSOMES - Abstract
A method of analysis of translation initiation complexes by toeprinting has recently acquired a wide application to investigate molecular mechanisms of translation initiation in eukaryotes. So far, this very fruitful approach was used when researchers did not aim to discriminate between patterns of toeprints for 48S and 80S translation initiation complexes. Here, using cap-dependent and internal ribosomal entry site (IRES)-dependent mRNAs, we show that the toeprint patterns for 48S and 80S complexes are distinct whether the complexes are assembled in rabbit reticulocyte lysate or from fully purified individual components. This observation allowed us to demonstrate for the first time a delay in the conversion of the 48S complex into the 80S complex for β-globin and encephalomyocarditis virus (EMCV) RNAs, and to assess the potential of some 80S antibiotics to block polypeptide elongation. Besides, additional selection of the authentic initiation codon among three consecutive AUGs that follow the EMCV IRES was revealed at steps subsequent to the location of the initiation codon by the 40S ribosomal subunit. [Copyright &y& Elsevier]
- Published
- 2003
- Full Text
- View/download PDF
45. Human Telomerase RNA: Telomerase Component or More?
- Author
-
Rubtsova, Maria and Dontsova, Olga
- Subjects
- *
TELOMERASE , *REVERSE transcriptase , *GENETIC regulation , *TELOMERES , *RNA , *RIBOSOMES - Abstract
Telomerase is a ribonucleoprotein complex that maintains the lengths of telomeres. Most studies of telomerase function have focused on the involvement of telomerase activation in the immortalization of cancer cells and cellular rejuvenation. However, some studies demonstrated that the results do not meet expectations for telomerase action in telomere maintenance. Recent results give reason to think that major telomerase components—the reverse transcriptase protein subunit and telomerase RNA—may participate in many cellular processes, including the regulation of apoptosis and autophagy, cell survival, pro-proliferative effects, regulation of gene expression, and protection against oxidative stress. However, the difficulties faced by scientist when researching telomerase component functions often reduce confidence in the minor effects observed in experiments. In this review, we focus on the analysis of the functions of telomerase components (paying more attention to the telomerase RNA component), both as a complex and as independent components, providing effects that are not associated with telomerase activity and telomere length maintenance. Despite the fact that the data on alternative roles of telomerase components look illusory, it would be wrong to completely reject the possibility of their involvement in other biological processes excluded from research/discussion. Investigations to improve the understanding of every aspect of the functioning of telomerase components will provide the basis for a more precise development of approaches to regulate cellular homeostasis, which is important for carcinogenesis and aging. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
46. Bizeolite Pt/ZSM-5:ZSM-12/Al2O3 catalyst for hydroisomerization of C-8 fraction with various ethylbenzene content.
- Author
-
Glotov, Aleksandr, Demikhova, Nataliya, Rubtsova, Maria, Melnikov, Dmitry, Tsaplin, Dmitriy, Gushchin, Pavel, Egazar'yants, Sergey, Maximov, Anton, Karakhanov, Eduard, and Vinokurov, Vladimir
- Subjects
- *
CATALYSTS , *X-ray spectroscopy , *MAGIC angle spinning , *ETHYLBENZENE , *CATALYST selectivity , *NUCLEAR magnetic resonance - Abstract
[Display omitted] • A new bizeolite Pt/ZSM-5:ZSM-12/Al 2 O 3 catalyst was designed for C-8 aromatics isomerization. • ZSM-5/ZSM-12 based Pt catalyst allows both intra- and intermolecular isomerization routes. • Ethylbenzene and m -xylene conversions over ZSM-5+ZSM-12 based catalyst are higher than over an industrial counterpart. A bizeolite catalyst based on ZSM-5 and ZSM-12 was designed to maximize the yield of valuable aromatics from ethylbenzene (EB) rich feedstocks for the first time. The catalyst Pt/ZSM-5:ZSM-12/Al 2 O 3 was characterized by X-ray diffraction (XRD), nitrogen physisorption, temperature-programmed desorption of ammonia (TPD-NH 3), transmission and scanning electron microscopy (TEM and SEM), X-ray fluorescence analysis (XRF), Fourier-transformed infrared spectroscopy (FTIR) and 27Al magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy. The catalyst and its components were tested in isomerization of an industrial C-8 aromatic cut (C8-Ar) and feedstocks with high EB content. The catalyst demonstrated higher conversion of EB and m -xylene (MX) compared with an industrial catalyst and higher selectivity to dealkylation products. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
47. Oligoglutamylation of E. coli ribosomal protein S6 is under growth phase control.
- Author
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Pletnev, Philipp I., Nesterchuk, Mikhail V., Rubtsova, Maria P., Serebryakova, Marina V., Dmitrieva, Ksenia, Osterman, Ilya A., Bogdanov, Alexey A., and Sergiev, Petr V.
- Subjects
- *
RIBOSOMES , *RIBOSOMAL proteins , *POST-translational modification , *BACTERIAL cultures , *GENETIC overexpression , *BIOSYNTHESIS , *ESCHERICHIA coli - Abstract
Ribosomal protein S6 in Escherichia coli is modified by ATP-dependent glutamate ligase RimK. Up to four glutamate residues are added to the C-terminus of S6 protein. In this work we demonstrated that unlike the majority of ribosome modifications in E. coli , oligoglutamylation of S6 protein is regulated and happens only in the stationary phase of bacterial culture. Only S6 protein incorporated into assembled small ribosomal subunits, but not newly made free S6 protein is a substrate for RimK protein. Overexpression of the rimK gene leads to the modification of S6 protein even in the exponential phase of bacterial culture. Thus, it is unlikely that any stationary phase specific factor is needed for the modification. We propose a model that S6 modification is regulated solely via the rate of ribosome biosynthesis at limiting concentration of RimK enzyme. • E. coli ribosomal protein S6 is oligoglutamylated by RimK. • Modification happens only in the stationary phase. • Assembled 30S subunit but not free S6 protein is modified. • A model is proposed for the regulation of S6 modification. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
48. Ag-Modified microfibrillar cellulose as support in composite phase change materials with enhanced thermal transfer properties.
- Author
-
Pirtsul, Alexandra E., Krainov, Alexey, Rubtsova, Maria I., Mendgaziev, Rais I., Cherednichenko, Kirill A., Vinokurov, Vladimir, and Voronin, Denis V.
- Subjects
- *
STEARIC acid , *HEAT transfer , *CELLULOSE fibers , *THERMAL properties , *PHASE change materials , *CELLULOSE , *HEAT storage , *THERMOGRAPHY - Abstract
• MFC was modified with Ag nanoparticles with a mass content of 13% • Adsorption of SA onto AgMFC fibers results in shape stable PCM composites. • Ag nanoparticles do not affect the phase transitions of SA on the fiber surface. • SA/AgMFC composites have improved heating and cooling rate comparing to SA/MFC ones. • The improved heat transfer in SA/AgMFC composites reduces their thermal stability. Composite phase change material was prepared via adsorption of stearic acid onto cellulose microfibrils modified with Ag nanoparticles. The latent heat storage capacity, cycling performance, thermal stability, and heating/cooling rate of the composites were studied with differential scanning calorimetry, thermogravimetric analysis, and thermal imaging and compared to those of the composite prepared by adsorption of stearic acid onto pure microfibrillar cellulose. The modification of cellulose with Ag nanoparticles improves the heating and cooling rate of the composites and promotes latent heat release during the solidification of stearic acid on the fiber surface. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
49. Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide technique.
- Author
-
Vasilkova, Daria V., Azhibek, Dulat M., Zatsepin, Timofei S., Naraikina, Yulia V., Prassolov, Vladimir S., Prokofjeva, Maria M., Zvereva, Maria I., and Rubtsova, Maria P.
- Subjects
- *
DYNAMICS , *TELOMERASE , *RNA , *ANTISENSE DNA , *ANTISENSE nucleic acids , *OLIGONUCLEOTIDES , *CHROMOSOMES - Abstract
Abstract: Telomeres are the nucleoprotein complexes that cap the linear chromosome ends. Telomerase is a ribonucleoprotein that maintains telomere length in stem, embryonic and cancer cells. Somatic cells don't contain active telomerase and telomere function as mitotic clock and telomere length determines the number of cell divisions. Telomerase RNA (TER) contains the template for telomere synthesis and serves as a structural scaffold for holoenzyme assembly. We compared different oligonucleotide based methods for telomerase RNA inhibition, such as antisense oligonucleotides, knockdown by transient siRNA transfection and silencing by miRNA derived from short expressed RNA hairpin in HEK293 cells. All of these methods were applied to different TER regions. Our results revealed that CR2/CR3 domain of TER is accessible in vitro and in vivo and could serve as an optimal site for oligonucleotide-based telomerase silencing. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
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