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LiDAR-unmanned aerial system (LiDAR-UAS) technology can accurately and efficiently obtain detailed and accurate three-dimensional spatial information of objects. The classification of objects in estuarine areas is highly important for management, planning, and ecosystem protection. Owing to the presence of slopes in estuarine areas, distinguishing between dense vegetation (lawns and trees) on slopes and the ground at the tops of slopes is difficult. In addition, the imbalance in the number of point clouds also poses a challenge for accurate classification directly from point cloud data. A multifeature-assisted and multilayer fused neural network (MLF-PointNet++) is proposed for LiDAR-UAS point cloud classification in estuarine areas. First, the 3D shape features that characterize the geometric characteristics of targets and the visible-band difference vegetation index (VDVI) that can characterize vegetation distribution are used as auxiliary features to enhance the distinguishability of dense vegetation (lawns and trees) on slopes and the ground at the tops of slopes. Second, to enhance the extraction of target spatial information and contextual relationships, the feature vectors output by different layers of set abstraction in the PointNet++ model are fused to form a combined feature vector that integrates low and high-level information. Finally, the focal loss function is adopted as the loss function in the MLF-PointNet++ model to reduce the effect of imbalance in the number of point clouds in each category on the classification accuracy. A classification evaluation was conducted using LiDAR-UAS data from the Moshui River estuarine area in Qingdao, China. The experimental results revealed that MLF-PointNet++ had an overall accuracy (OA), mean intersection over union (mIOU), kappa coefficient, precision, recall, and F1-score of 0.976, 0.913, 0.960, 0.953, 0.953, and 0.953, respectively, for object classification in the three representative areas, which were better than the corresponding values for the classification methods of random forest, BP neural network, Naive Bayes, PointNet, PointNet++, and RandLA-Net. The study results provide effective methodological support for the classification of objects in estuarine areas and offer a scientific basis for the sustainable development of these areas. [ABSTRACT FROM AUTHOR]

Background: Myasthenia gravis (MG) is an autoimmune disease characterized by pathogenic antibodies that target structures of the neuromuscular junction. The evidence suggests that the regulation of long noncoding RNAs (lncRNAs) that is mediated by transcription factors (TFs) plays a key role in the pathophysiology of MG. Nevertheless, the detailed molecular mechanisms of lncRNAs in MG remain largely undetermined. Methods: Using microarray analysis, we analyzed the lncRNA levels in MG. By bioinformatics analysis, LINC01566 was found to potentially play an important role in MG. First, qRT‒PCR was performed to verify the LINC1566 expressions in MG patients. Then, fluorescence in situ hybridization was conducted to determine the localization of LINC01566 in CD4 + T cells. Finally, the impact of LINC01566 knockdown or overexpression on CD4 + T-cell function was also analyzed using flow cytometry and CCK-8 assay. A dual-luciferase reporter assay was used to validate the binding of the TF FOSL1 to the LINC01566 promoter. Results: Based on the lncRNA microarray and differential expression analyses, we identified 563 differentially expressed (DE) lncRNAs, 450 DE mRNAs and 19 DE TFs in MG. We then constructed a lncRNA-TF-mRNA network. Through network analysis, we found that LINC01566 may play a crucial role in MG by regulating T-cell-related pathways. Further experiments indicated that LINC01566 is expressed at low levels in MG patients. Functionally, LINC01566 is primarily distributed in the nucleus and can facilitate CD4 + T-cell apoptosis and inhibit cell proliferation. Mechanistically, we hypothesized that LINC01566 may negatively regulate the expressions of DUSP3, CCR2, FADD, SIRPB1, LGALS3 and SIRPB1, which are involved in the T-cell activation pathway, to further influence the cellular proliferation and apoptosis in MG. Moreover, we found that the effect of LINC01566 on CD4 + T cells in MG was mediated by the TF FOSL1, and in vitro experiments indicated that FOSL1 can bind to the promoter region of LINC01566. Conclusions: In summary, our research revealed the protective roles of LINC01566 in clinical samples and cellular experiments, illustrating the potential roles and mechanism by which FOSL1/LINC01566 negatively regulates CD4 + T-cell activation in MG. [ABSTRACT FROM AUTHOR]

Nucleic acids, including DNA and RNA, have been considered as powerful and functional biomaterials owing to their programmable structure, good biocompatibility, and ease of synthesis. However, traditional nucleic acid-based probes have always suffered from inherent limitations, including restricted cell internalization efficiency and structural instability. In recent years, DNA nanotechnology has shown great promise for the applications of bioimaging and drug delivery. The attractive superiorities of DNA nanostructures, such as precise geometries, spatial addressability, and improved biostability, have enabled them to be a novel category of nucleic acid delivery systems for biomedical applications. In this review, we introduce the development of DNA nanotechnology, and highlight recent advances of DNA nanostructure-based delivery systems for cellular imaging and therapeutic applications. Finally, we propose the challenges as well as opportunities for the future development of DNA nanotechnology in biomedical research. [ABSTRACT FROM AUTHOR]

Strain-hardened austenitic stainless steel (γ-SS) is extensively used in high-pressure hydrogen systems after welding. The weld may experience varying degrees of hydrogen embrittlement (HE) with different levels of pre-strain, which seriously threatens the reliability of hydrogen systems. Therefore, it is vital to research the effect of pre-strain on hydrogen-induced cracking of γ-SS welds. In this research, the impact of hydrogen on the mechanical properties of type 304 γ-SS plasma arc welding (PAW) welds with different levels of pre-strain are studied through slow strain rate tensile (SSRT) test. The pre-strain of the weld after hydrogen charging is optimized, then the causes of hydrogen-induced cracking are illustrated by microstructure analysis. The weld metal zone (WMZ) has a greater HE susceptibility than the base metal zone (BMZ). The HE susceptibility of the weld can decrease after solution-treated. The fracture of the weld with pre-strain of 5% is mainly due to dislocation and ferrite increasing the HE susceptibility. When the pre-strain is above 10%, the weld metal and the base metal have undergone a severe transformation from austenite to α′ martensite. The content of α′ martensite rises with the rising pre-strain. The HE susceptibility of the weld is greater than the base metal due to the influence of dislocations and ferrite. The brittle fracture is initiated primarily at the phase boundary. The strength of welds can be improved while keeping good HE resistance with pre-strain of 5%. [Display omitted] • The mechanical properties of the welds can be improved while keeping good resistance to hydrogen embrittlement with pre-strain of 5%. • The existence of ferrite reduces the relative elongation and relative reduction of area of the welds. • The brittle fracture of the weld is initiated primarily at the phase boundary between austenite and α′ martensite. [ABSTRACT FROM AUTHOR]

Six new compounds, (7R,8S,8′R)-balanophorone (1), (7′S,8′R,8R)-yunnanensin A (2), (3S)-thunberginol C (3), (8R,8′R)-maninsigin B (4), (7S,8R)-4,7,8-dihydroxy-9,9-dimethyl-chroman (5), and 4-hydroxy-1-(4-hydroxy-3-methoxyphenyl)butan-1-one (6), along with eight known compounds (7–14), were isolated from the herbaceous stems of Ephedra intermedia Schrenket C. A. Meyer. Their structures were elucidated based on their spectroscopic (MS, NMR, IR, and UV) data, and their absolute configurations were determined by comparing their calculated and experimental electronic circular dichroic (ECD) spectra. Moreover, compounds 1 and 3–6 were evaluated for their ability to protect human pulmonary epithelial cells (BEAS-2B) from injury induced by lipopolysaccharide (LPS) in vitro. The results showed that compound 6 exhibited a significant protective effect against LPS-induced injury in BEAS-2B, and compound 5 exhibited a slightly protective effect at the concentration of 10 μM. [ABSTRACT FROM AUTHOR]

Mg2SiO4 and MgSiO3 ceramics with superior microwave dielectric properties are considered to be promising candidates for 5G applications. However, a slight deviation from the stoichiometric Mg/Si ratio will significantly influence their microwave dielectric properties, which will hinder their practical applications. In this work, the xMgO-SiO2 (x = 1~2) ceramics were synthesized by a solid-state reaction method. The influence of the Mg/Si ratio x on the crystalline phase, microstructure, and microwave dielectric properties was investigated through X-ray diffraction (XRD), a scanning electron microscope (SEM), and the resonant cavity method. The XRD patterns revealed the coexistence of Mg2SiO4 and MgSiO3 within the x range of 1~2, which was further demonstrated by the energy-dispersive X-ray spectra. The SEM images show a typical polycrystalline morphology of ceramics with an inhomogeneous grain size distribution. It is found that the microwave dielectric properties fluctuate at both sides of the x range while those remain relatively stable with minor changes at the intermediate components, indicating an obvious low composition dependence helpful for practical applications. Further, a demonstrator of a microstrip patch antenna for 5G applications using the 1.5MgO-SiO2 ceramic was designed and fabricated, and a return loss of −16.2 dB was demonstrated, which demonstrated the potential applications. [ABSTRACT FROM AUTHOR]

Microneedles, especially hollow microneedles (HMNs), play an important role in drug delivery, but most of the current HMNs are manufactured based on silicon microfabrication (lithography, etching, etc.), which are slightly conservative due to the lack of low‐cost, batch‐scale and customized preparation approach, especially for the HMNs with flexible substrate. For the first time, we propose the use of a high‐precision 3D printed master mold followed by a dual‐molding process for the preparation of HMNs with different shapes, heights, and inner and outer diameters to satisfy different drug delivery needs. The 3D printed master mold and negative mold can be reused, thereby significantly reducing the cost. HMNs are based on biocompatible materials, such as heat‐curing polymers or light‐curing resins. The thickness and rigidity/flexibility characteristics of the substrate can be customized for different applications. The drug delivery efficiency of the fabricated HMNs was verified by the in situ treatment of psoriasis on the backs of mice, which required only a 0.1‐fold oral dose to achieve similar efficacy, and the associated side effects and drug toxicity were reduced. Thus, this dual‐molding process can reinvigorate HMNs development. [ABSTRACT FROM AUTHOR]

In this study, dense composites of xNaCl-(1−x)Ni0.5Zn0.5Fe2O4 (referred to as NaCl-NZO) and xH3BO3-(0.8−x)Ni0.5Zn0.5Fe2O4-0.2NaCl (referred to as HB-NZO-NaCl) were prepared using the cold sintering process. The objective was to investigate the cold sintering procedure for fabricating ferrite composite ceramics with comparable permeability and dielectric constants suitable for radio-frequency electronic device applications. Optimal cold sintering conditions were determined as 200 °C/30 min/500 MPa with a relative density of 95% for NaCl-NZO and 120 °C/30 min/300 MPa with a relative density of 95.4% for HB-NZO-NaCl. X-ray diffraction and scanning electron microscope analyses confirmed the absence of secondary phases. The resulting composite ceramics showed promising characteristics, with the 0.5NaCl-0.5NZO composition exhibiting a dielectric constant of 6.2 @ 100 MHz, dielectric loss of 0.02 @ 100 MHz, permeability of 2.5 @ 100 MHz, and magnetic loss of 0.001 @ 100 MHz. Similarly, the 0.3HB-0.5NZO-0.2NaCl composition displayed a dielectric constant of 5.9 @ 100 MHz, dielectric loss of 0.02 @ 100 MHz, permeability of 5.1 @ 100 MHz, and magnetic loss of 5 × 10−4 @ 100 MHz. These findings indicate potential applications in wireless communication. [ABSTRACT FROM AUTHOR]

The mechanical properties of materials deteriorate when hydrogen embrittlement (HE) occurs, seriously threatening the reliability and durability of the hydrogen system. Therefore, it is important to summarize the status and development trends of research on HE. This study reviewed 6676 publications concerned with HE from 1997 to 2022 based on the Web of Science Core Collection. VOSviewer was used to conduct the bibliometric analysis and produce visualizations of the publications. The results showed that the number of publications on HE increased after 2007, especially between 2017 and 2019. Japan was the country with the highest numbers of productive authors and citations of publications, and the total number of citations of Japanese publications was 24,589. Kyushu University was the most influential university, and the total number of citations of Kyushu University publications was 7999. Akiyama was the most prolific and influential author, publishing 88 publications with a total of 2565 citations. The USA, South Korea and some European countries are also leading in HE research; these countries have published more than 200 publications. It was also found that the HE publications generally covered five topics: "Hydrogen embrittlement in different materials", "Effect of hydrogen on mechanical properties of materials", "Effect of alloying elements or microstructure on hydrogen embrittlement", "Hydrogen transport", and "Characteristics and mechanisms of hydrogen related failures". Research hotspots included "Fracture failure behavior and analysis", "Microstructure", "Hydrogen diffusion and transport", "Mechanical properties", "Hydrogen resistance", and so on. These covered the basic methods and purposes of HE research. Finally, the distribution of the main subject categories of the publications was determined, and these categories covered various topics and disciplines. This study establishes valuable reference information for the application and development of HE research and provides a convenient resource to help researchers and scholars understand the development trends and research directions in this field. [ABSTRACT FROM AUTHOR]

Monitoring sleep conditions is of importance for sleep quality evaluation and sleep disease diagnosis. Accurate respiration detection provides key information about sleep conditions. Here, we propose a perforated temperature sensor that can be worn below the nasal cavity to monitor breath. The sensing system consists of two perforated temperature sensors, signal conditioning circuits, a transmission module, and a supporting analysis algorithm. The perforated structure effectively enhances the sensitivity of the system and shortens the response time. The sensor's response time is 0.07 s in air and sensitivity is 1.4‰°C−1. The device can achieve a monitoring respiratory temperature range between normal room temperature and 40 °C. The simple and standard micromachining process ensures low cost and high reproducibility. We achieved the monitoring of different breathing patterns, such as normal breathing, panting, and apnea, which can be applied to sleep breath monitoring and exercise information recording. [ABSTRACT FROM AUTHOR]

A new flavonoid, saffloflavanside (1), a new sesquiterpene, safflomegastigside (2), and a new amide, saffloamide (3), together with twenty-two known compounds (4–25), were isolated from the flowers of Carthamus tinctorius L. Their structures were determined based on interpretation of their spectroscopic data and comparison with those reported in the literature. The protective effects against lipopolysaccharide (LPS)-stimulated damage on human normal lung epithelial (BEAS-2B) cells of the compounds were evaluated using MTT assay and cellular immunofluorescence assay. The results showed that compounds 2–3, 8–11, and 15–19 exhibited protective effects against LPS-induced damage to BEAS-2B cells. Moreover, compounds 2–3, 8–11, and 15–19 can significantly downregulate the level of nuclear translocation of NF-κB p-p65. In summary, this study revealed chemical constituents with lung protective activity from C. tinctorius, which may be developed as a drug for the treatment of lung injury. [ABSTRACT FROM AUTHOR]

Results show that human replication protein A (RPA) specifically binds to damaged double-stranded DNA and the specificity is dependent on the zinc-finger domain of RPA-70. Data further indicate that RPA exhibits highest affinity for pyrimidine-pirimidone photoproducts of the damaged DNA.

[Display omitted] • Two new diarylheptanoids (1 – 2) and a new phenylhexanol derivative (3), along with seven known compounds (4 – 10) were isolated from the bulbils of Dioscorea opposita Thunb.. • The absolute configurations of compounds 1 – 2 were deduced by comparison of the experimental and calculated ECD spectra. • The α -glucosidase inhibitory effects of all the isolated compounds were evaluated. • Molecular docking and molecular dynamics simulations of compound 7 bound α -glucosidase were performed Two previously undescribed diarylheptanoids, diosniponol E (1) and F (2), and a new phenylhexanol derivative, (rel-2 R ,4 R)-6-(4-hydroxy-3-methoxyphenyl)-hexane-1,2,4-triol (3), together with seven known compounds, were isolated from the bulbils of Dioscorea opposita Thunb.. Their structures were elucidated by HRESIMS and 1D/2D NMR as well as by comparison with the literature, and absolute configurations of compounds 1 and 2 were deduced by comparison of their experimental and calculated electronic circular dichroic (ECD) spectra. All the isolated compounds were evaluated in vitro for their α -glucosidase inhibitory activity. The results indicated that compounds 3 and 7 exhibited inhibitory effect against α -glucosidase with the IC 50 values of 16.2 ± 2.2 and 8.7 ± 1.6 μ M, respectively. Molecular docking experiments were performed to identify the probable binding mode of compound 7 in the binding sites of α -glucosidase and molecular dynamics simulations were performed to evaluate the stability over time of the main interactions observed in docking experiments. [ABSTRACT FROM AUTHOR]

Two previously undescribed alkaloids, 4,4′-dihydroxy-3-methoxy-paucine-4′-O-β- D -glucopyranoside and (S)-2-(1,3-propanediol-2-yl)-isococlaurine, and three new lignans, (7R,8S)-9,9′-dihydroxy-3,4-dimethoxy-7,8-dihydrobenzofuran lignan, (7R,8S)-4,9,9′-trihydroxy-3,3′-dimethoxy-9′-acetoxy-7,8-dihydrobenzofuran lignan-4-O-β- D -glucopyranoside, and (7S,8R)-4,9,9′-trihydroxy-3,3′,5-trimethoxy-9′-acetoxy-7,8-dihydro-benzofuran lignan-4-O-β- D -glucopyranoside, together with eleven known compounds, were isolated from the flower buds of Magnolia biondii Pamp. Their structures were determined by extensive spectroscopic analysis, and their absolute configurations were deduced by analysis of optical rotation and electronic circular dichroic (ECD) spectra. All the isolated compounds were evaluated in vitro for their acetylcholinesterase (AChE) inhibitory activity. As a result, it was found that (+)-isococlaurine exhibited the strongest AChE inhibitory effect. Molecular docking experiments were carried out to identify the probable binding mode of this compound in the binding sites of AChE and molecular dynamics simulations were performed to evaluate the stability over time of the main interactions observed during docking calculations. [ABSTRACT FROM AUTHOR]

[Display omitted] • Two new and two known flavonoids were isolated from the thorns of Gleditsia sinensis. • The absolute configurations were deduced by interpretations of the ECD spectra. • The inhibition effects against the NRK 52e cells apoptosis induced by LPS were detected. Two new flavonoids, (2 R ,3 R ,7′′ R ,8′' S)-5′′-methoxyl-silychristin A, (2 R ,3 R ,7′′ S ,8′′ R)-5′′-methoxyl-silychristin A, and two known analogues were isolated from the thorns of Gleditsia sinensis. Their structures were determined by spectroscopic data analysis. The absolute configurations of the new compounds were deduced by interpretation of the ECD spectra. The cytoprotective effects of the isolated compounds against lipopolysaccharide (LPS)-induced apoptosis in normal rat kidney tubule epithelioid (NRK 52e) cells were investigated in vitro and the results showed that compounds 2 and 3 exhibited moderate protective effects with EC 50 values of 10.5 and 8.3 μM, respectively. [ABSTRACT FROM AUTHOR]