25 results on '"Ponzetta, Andrea"'
Search Results
2. Major alterations in the mononuclear phagocyte landscape associated with COVID-19 severity
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Karolinska KI/K COVID-19 Study Group, Kvedaraite, Egle, Hertwig, Laura, Sinha, Indranil, Ponzetta, Andrea, Myrberg, Ida Hed, Lourda, Magda, Dzidic, Majda, Akber, Mira, Klingström, Jonas, Folkesson, Elin, Muvva, Jagadeeswara Rao, Chen, Puran, Gredmark-Russ, Sara, Brighenti, Susanna, Norrby-Teglund, Anna, Eriksson, Lars I., Rooyackers, Olav, Aleman, Soo, Strålin, Kristoffer, Ljunggren, Hans-Gustaf, Ginhoux, Florent, Björkström, Niklas K., Henter, Jan-Inge, and Svensson, Mattias
- Published
- 2021
3. Natural killer cells and unconventional T cells in COVID-19
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Björkström, Niklas K and Ponzetta, Andrea
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- 2021
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4. Complement activation promoted by the lectin pathway mediates C3aR-dependent sarcoma progression and immunosuppression
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Magrini, Elena, Di Marco, Sabrina, Mapelli, Sarah N., Perucchini, Chiara, Pasqualini, Fabio, Donato, Alessia, Guevara Lopez, Maria de la Luz, Carriero, Roberta, Ponzetta, Andrea, Colombo, Piergiuseppe, Cananzi, Ferdinando, Supino, Domenico, Reis, Edimara S., Peano, Clelia, Inforzato, Antonio, Jaillon, Sebastien, Doni, Andrea, Lambris, John D., Mantovani, Alberto, and Garlanda, Cecilia
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- 2021
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5. Tissue-specific nonheritable influences drive endometrial immune system variation.
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Bister, Jonna, Filipovic, Iva, Sun, Dan, Crona-Guterstam, Ylva, Cornillet, Martin, Ponzetta, Andrea, Michaëlsson, Jakob, Gidlöf, Sebastian, Ivarsson, Martin A., Strunz, Benedikt, and Björkström, Niklas K.
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IMMUNE system ,MONOZYGOTIC twins ,CYTOMEGALOVIRUS diseases ,BLOOD cells ,TWIN studies - Abstract
Although human twin studies have revealed the combined contribution of heritable and environmental factors in shaping immune system variability in blood, the contribution of these factors to immune system variability in tissues remains unexplored. The human uterus undergoes constant regeneration and is exposed to distinct environmental factors. To assess uterine immune system variation, we performed a system-level analysis of endometrial and peripheral blood immune cells in monozygotic twins. Although most immune cell phenotypes in peripheral blood showed high genetic heritability, more variation was found in endometrial immune cells, indicating a stronger influence by environmental factors. Cytomegalovirus infection was identified to influence peripheral blood immune cell variability but had limited effect on endometrial immune cells. Instead, hormonal contraception shaped the local endometrial milieu and immune cell composition with minor influence on the systemic immune system. These results highlight that the magnitude of human immune system variation and factors influencing it can be tissue specific. Editor's summary: Baseline differences in the immune system between individuals are driven by both heritable and nonheritable, environmental factors. By analyzing endometrial and peripheral blood immune cells collected from monozygotic twins, Bister et al. measured the tissue-specific contributions of nonheritable effects on immune system variation. Although peripheral blood immune cells were largely shaped by genetics, environmental factors including hormonal contraception contributed more strongly to the variation in endometrial immune cells and soluble proteome. Together, these findings demonstrate that the human immune system can be highly variable and driven by nonheritable factors in a tissue-specific manner. —Claire Olingy [ABSTRACT FROM AUTHOR]
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- 2024
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6. Role of bacteria and microbial metabolites in immune modulation during early life.
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Oldereid, Tine Simensen, Jiang, Xiaojun, Nordhus, Kathrine Sivertsen, Ponzetta, Andrea, Bjørnholt, Jørgen Vildershøj, Björkström, Niklas K., Melum, Espen, and Rasmussen, Henrik
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MICROBIAL metabolites ,IMMUNOREGULATION ,LYMPHOID tissue ,ORAL drug administration ,MUCOUS membranes - Abstract
Host–microbiome interplay from birth is essential for immune imprinting and tuning. Live gut microbes and microbial‐derived metabolites regulate the development and modulation of the immune system, but whether microbial metabolites solely are sufficient to induce immune maturation remains unclear. Sterile faecal filtrates (FFT) were generated from murine gut contents. Newborn germ‐free (GF) mice were treated twice daily with FFT (GF‐FFT) or saline (GF‐NaCl) from post‐natal day 5 until 4 weeks of age. A third group of GF neonates were conventionalized by the transfer of caecal microbiota with live gut microbes. Host immune compartments were comprehensively immunophenotyped and systemically analysed in all available immune‐related organs using flow cytometry. Oral FFT was associated with reduced survival among neonates (n = 7/19; 36.8% mortality), while saline treatment was well tolerated (n = 1/17, 5.9% mortality). Four‐week‐old FFT‐treated pups were comparable in body weight to GF‐NaCl, and the major B‐cell, conventional T‐cell and unconventional T‐cell subsets were unchanged from saline‐treated mice. Live bacteria administered during early life induced clear changes in proportions of B cells, T cells and T‐cell subsets in all mucosal tissues and secondary lymphoid organs compared to GF‐FFT, including restoration of intestinal natural killer T (NKT) cells with characteristics similar to conventional pups. Our findings show that oral administration of a FFT made of microbial metabolites, antigens and bacteriophages alone is insufficient to induce normal immune development elicited by the presence of live bacteria. Reduced survival during neonatal FFT treatment suggests a potential bioactive attribute of sterile faecal filtrates. [ABSTRACT FROM AUTHOR]
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- 2024
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7. IL-1R8 is a checkpoint in NK cells regulating anti-tumour and anti-viral activity
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Molgora, Martina, Bonavita, Eduardo, Ponzetta, Andrea, Riva, Federica, Barbagallo, Marialuisa, Jaillon, Sbastien, Popovi, Branka, Bernardini, Giovanni, Magrini, Elena, Gianni, Francesca, Zelenay, Santiago, Jonji, Stipan, Santoni, Angela, Garlanda, Cecilia, and Mantovani, Alberto
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Interleukins -- Health aspects ,Killer cells -- Health aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Martina Molgora [1]; Eduardo Bonavita [1]; Andrea Ponzetta [1]; Federica Riva [2]; Marialuisa Barbagallo [1]; Sbastien Jaillon [1, 3]; Branka Popovi [4]; Giovanni Bernardini [5, 6]; Elena Magrini [1]; [...]
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- 2017
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8. CX3CL1 protects neurons against excitotoxicity enhancing GLT-1 activity on astrocytes
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Catalano, Myriam, Lauro, Clotilde, Cipriani, Raffaela, Chece, Giuseppina, Ponzetta, Andrea, Di Angelantonio, Silvia, Ragozzino, Davide, and Limatola, Cristina
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- 2013
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9. Distinct T cell subsets in adipose tissue are associated with obesity.
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Haugstøyl, Martha E., Cornillet, Martin, Strand, Kristina, Stiglund, Natalie, Sun, Dan, Lawrence‐Archer, Laurence, Hjellestad, Iren D., Sparrelid, Ernesto, Busch, Christian, Hjelmesæth, Jøran, Hertel, Jens K., Ponzetta, Andrea, Mellgren, Gunnar, Fernø, Johan, and Björkström, Niklas K.
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ADIPOSE tissues ,T cells ,FAT cells ,BLOOD cells ,METABOLIC disorders - Abstract
Adipose tissue inflammation is a driving factor for the development of obesity‐associated metabolic disturbances, and a role of adipose tissue T cells in initiating the pro‐inflammatory signaling is emerging. However, data on human adipose tissue T cells in obesity are limited, reflected by the lack of phenotypic markers to define tissue‐resident T cell subsets. In this study, we performed a deep characterization of T cells in blood and adipose tissue depots using multicolor flow cytometry and RNA sequencing. We identified distinct subsets of T cells associated with obesity expressing the activation markers, CD26 and CCR5, and obesity‐specific genes that are potentially engaged in activating pro‐inflammatory pathway, including ceramide signaling, autophagy, and IL‐6 signaling. These findings increase our knowledge on the heterogeneity of T cells in adipose tissue and on subsets that may play a role in obesity‐related pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2023
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10. CX3CR1 expression defines 2 KLRG1+ mouse NK-cell subsets with distinct functional properties and positioning in the bone marrow
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Sciumè, Giuseppe, De Angelis, Giulia, Benigni, Giorgia, Ponzetta, Andrea, Morrone, Stefania, Santoni, Angela, and Bernardini, Giovanni
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- 2011
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11. Occurrence and significance of tumor-associated neutrophils in patients with colorectal cancer
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Galdiero, Maria Rosaria, Bianchi, Paolo, Grizzi, Fabio, Di Caro, Giuseppe, Basso, Gianluca, Ponzetta, Andrea, Bonavita, Eduardo, Barbagallo, Marialuisa, Tartari, Silvia, Polentarutti, Nadia, Malesci, Alberto, Marone, Gianni, Roncalli, Massimo, Laghi, Luigi, Garlanda, Cecilia, Mantovani, Alberto, and Jaillon, Sébastien
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- 2016
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12. Chemotherapy-induced neutropenia elicits metastasis formation in mice by promoting proliferation of disseminated tumor cells.
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Russo, Massimo, Panini, Nicolò, Fabbrizio, Paola, Formenti, Laura, Becchetti, Riccardo, Matteo, Cristina, Meroni, Marina, Nastasi, Claudia, Cappelleri, Andrea, Frapolli, Roberta, Nardo, Giovanni, Scanziani, Eugenio, Ponzetta, Andrea, Bani, Maria Rosa, Ghilardi, Carmen, and Giavazzi, Raffaella
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CANCER invasiveness ,CHEMOTHERAPY complications ,NEUTROPENIA ,OXYGENATORS ,METASTASIS - Abstract
Chemotherapy is the standard of care for most malignancies. Its tumor debulking effect in adjuvant or neoadjuvant settings is unquestionable, although secondary effects have been reported that paradoxically promote metastasis. Chemotherapy affects the hematopoietic precursors leading to myelosuppression, with neutropenia being the main hematological toxicity induced by cytotoxic therapy. We used renal and lung murine tumor models metastatic to the lung to study chemotherapy-induced neutropenia (CIN) in the metastatic process. Cyclophosphamide and doxorubicin, two myelosuppressive drugs, but not cisplatin, increased the burden of artificial metastases to the lung, by reducing neutrophils. This effect was recapitulated by treatment with anti-Ly6G, the selective antibody-mediated neutrophil depletion that unleashed the formation of lung metastases in both artificial and spontaneous metastasis settings. The increased cancer dissemination was reversed by granulocyte-colony stimulating factor-mediated boosting of neutrophils in combination with chemotherapy. CIN affected the early metastatic colonization of the lung, quite likely promoting the proliferation of tumor cells extravasated into the lung at 24–72 hours. CIN did not affect the late events of the metastatic process, with established metastasis to the lung, nor was there any effect on the release of cancer cells from the primary, whose growth was, in fact, somewhat inhibited. This work suggests a role of neutrophils associated to a common cancer treatment side effect and claims a deep dive into the relationship between chemotherapy-induced neutropenia and metastasis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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13. COVID‐19‐specific metabolic imprint yields insights into multiorgan system perturbations.
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Cornillet, Martin, Strunz, Benedikt, Rooyackers, Olav, Ponzetta, Andrea, Chen, Puran, Muvva, Jagadeeswara Rao, Akber, Mira, Buggert, Marcus, Chambers, Benedict J., Dzidic, Majda, Filipovic, Iva, Gorin, Jean‐Baptiste, Gredmark‐Russ, Sara, Hertwig, Laura, Klingström, Jonas, Kokkinou, Efthymia, Kvedaraite, Egle, Lourda, Magda, Mjösberg, Jenny, and Maucourant, Christopher
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SARS-CoV-2 ,COVID-19 - Abstract
Corona disease 2019 (COVID‐19) affects multiple organ systems. Recent studies have indicated perturbations in the circulating metabolome linked to COVID‐19 severity. However, several questions pertain with respect to the metabolome in COVID‐19. We performed an in‐depth assessment of 1129 unique metabolites in 27 hospitalized COVID‐19 patients and integrated results with large‐scale proteomic and immunology data to capture multiorgan system perturbations. More than half of the detected metabolic alterations in COVID‐19 were driven by patient‐specific confounding factors ranging from comorbidities to xenobiotic substances. Systematically adjusting for this, a COVID‐19‐specific metabolic imprint was defined which, over time, underwent a switch in response to severe acute respiratory syndrome coronavirus‐2 seroconversion. Integration of the COVID‐19 metabolome with clinical, cellular, molecular, and immunological severity scales further revealed a network of metabolic trajectories aligned with multiple pathways for immune activation, and organ damage including neurological inflammation and damage. Altogether, this resource refines our understanding of the multiorgan system perturbations in severe COVID‐19 patients. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Imprint of unconventional T‐cell response in acute hepatitis C persists despite successful early antiviral treatment.
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Du, Yanqin, Khera, Tanvi, Strunz, Benedikt, Deterding, Katja, Todt, Daniel, Woller, Norman, Engelskircher, Sophie Anna, Hardtke, Svenja, Port, Kerstin, Ponzetta, Andrea, Steinmann, Eike, Cornberg, Markus, Hengst, Julia, Björkström, Niklas K., and Wedemeyer, Heiner
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HEPATITIS C ,CHRONIC hepatitis C ,T cells ,HEPATITIS C virus ,HEPATITIS - Abstract
Unconventional T cells (UTCs) are a heterogeneous group of T cells that typically exhibit rapid responses toward specific antigens from pathogens. Chronic hepatitis C virus (HCV) infection causes dysfunction of several subsets of UTCs. This altered phenotype and function of UTCs can persist over time even after direct‐acting antiviral (DAA)‐mediated clearance of chronic HCV. However, it is less clear if and how UTCs respond in acute, symptomatic HCV infection, a rare clinical condition, and if rapid DAA treatment of such patients reverses the caused perturbations within UTCs. Here, we comprehensively analyzed the phenotype and reinvigoration capacity of three major UTC populations, mucosal‐associated invariant T (MAIT) cells, γδ T cells, and CD4 and CD8 double‐negative αβ T cells (DNT cells) before, during, and after DAA‐mediated clearance of acute symptomatic HCV infection. Furthermore, MAIT cell functionality was systematically studied. We observed a reduced frequency of MAIT cells. However, remaining cells presented with a near‐to‐normal phenotype in acute infection, which contrasted with a significant dysfunction upon stimulation that was not restored after viral clearance. Notably, DNT and γδ T cells displayed a strong activation ex‐vivo in acute HCV infection, which subsequently normalized during the treatment. In addition, DNT cell activation was specifically associated with liver inflammation and inflammatory cytokines. Altogether, these data provide evidence that UTCs respond in a cell type‐specific manner during symptomatic HCV infection. However, even if early treatment is initiated, long‐lasting imprints within UTCs remain over time. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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15. Major alterations in the mononuclear phagocyte landscape associated with COVID-19 severity.
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Kvedaraite, Egle, Hertwig, Laura, Sinha, Indranil, Ponzetta, Andrea, Myrberg, Ida Hed, Lourda, Magda, Dzidic, Majda, Akber, Mira, Klingström, Jonas, Folkesson, Elin, Muvva, Jagadeeswara Rao, Chen, Puran, Gredmark-Russ, Sara, Brighenti, Susanna, Norrby-Teglund, Anna, Eriksson, Lars I., Rooyackers, Olav, Soo Aleman, Strålin, Kristoffer, and Ljunggren, Hans-Gustaf
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MACROPHAGES ,COVID-19 ,VIRUS diseases ,SUPPRESSOR cells ,SARS-CoV-2 - Abstract
Dendritic cells (DCs) and monocytes are crucial mediators of innate and adaptive immune responses during viral infection, but misdirected responses by these cells may contribute to immunopathology. Here, we performed high-dimensional flow cytometryanalysis focusing on mononuclear phagocyte (MNP) lineages in SARS-CoV-2-infected patients with moderate and severe COVID-19. We provide a deep and comprehensive map of the MNP landscape in COVID-19. A redistribution of monocyte subsets toward intermediate monocytes and a general decrease in circulating DCs was observed in response to infection. Severe disease coincided with the appearance of monocytic myeloid-derived suppressor cell-like cells and a higher frequency of pre-DC2. Furthermore, phenotypic alterations in MNPs, and their late precursors, were cell-line-age-specific and associated either with the general response against SARS-CoV-2 or COVID-19 severity. This included an interferon-imprint in DC1s observed in all patients and a decreased expression of the coinhibitory molecule CD200R in pre-DCs, DC2s, and DC3 subsets of severely sick patients. Finally, unsupervised analysis revealed that the MNP profile, alone, pointed to a cluster of COVID-19 nonsurvivors. This study provides a reference for the MNP response to SARS-CoV-2 infection and unravels mononuclear phagocyte dysregulations associated with severe COVID-19. [ABSTRACT FROM AUTHOR]
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- 2021
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16. MAIT cell activation and dynamics associated with COVID-19 disease severity.
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Parrot, Tiphaine, Gorin, Jean-Baptiste, Ponzetta, Andrea, Maleki, Kimia T., Kammann, Tobias, Emgård, Johanna, Perez-Potti, André, Sekine, Takuya, Rivera-Ballesteros, Olga, Gredmark-Russ, Sara, Rooyackers, Olav, Folkesson, Elin, Eriksson, Lars I., Norrby-Teglund, Anna, Ljunggren, Hans-Gustaf, Björkström, Niklas K., Aleman, Soo, Buggert, Marcus, Klingström, Jonas, and Strålin, Kristoffer
- Abstract
Severe COVID-19 is characterized by excessive inflammation of the lower airways. The balance of protective versus pathological immune responses in COVID-19 is incompletely understood. Mucosa-associated invariant T (MAIT) cells are antimicrobial T cells that recognize bacterial metabolites, and can also function as innate-like sensors and mediators of antiviral responses. Here, we investigated the MAIT cell compartment in COVID-19 patients with moderate and severe disease, as well as in convalescence. We show profound and preferential decline in MAIT cells in the circulation of patients with active disease paired with strong activation. Furthermore, transcriptomic analyses indicated significant MAIT cell enrichment and pro-inflammatory IL-17A bias in the airways. Unsupervised analysis identified MAIT cell CD69
high and CXCR3low immunotypes associated with poor clinical outcome. MAIT cell levels normalized in the convalescent phase, consistent with dynamic recruitment to the tissues and later release back into the circulation when disease is resolved. These findings indicate that MAIT cells are engaged in the immune response against SARS-CoV-2 and suggest their possible involvement in COVID-19 immunopathogenesis. [ABSTRACT FROM AUTHOR]- Published
- 2020
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17. Natural killer cell immunotypes related to COVID-19 disease severity.
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Maucourant, Christopher, Filipovic, Iva, Ponzetta, Andrea, Aleman, Soo, Cornillet, Martin, Hertwig, Laura, Strunz, Benedikt, Lentini, Antonio, Reinius, Björn, Brownlie, Demi, Cuapio, Angelica, Ask, Eivind Heggernes, Hull, Ryan M., Haroun-Izquierdo, Alvaro, Schaffer, Marie, Klingström, Jonas, Folkesson, Elin, Buggert, Marcus, Sandberg, Johan K., and Eriksson, Lars I.
- Abstract
Activated NK cells in severe COVID-19: Natural killer (NK) cells are cytotoxic lymphocytes that provide innate immune defense against viral infections and cancer, but little is known about their involvement in the host response to COVID-19. Maucourant et al. used high-dimensional flow cytometry to characterize NK cells in patients with moderate or severe COVID-19. SARS-CoV-2 infection was associated with fewer blood NK cells but a higher activation state in circulating NK cells. Severe COVID-19 resulted in an increase in "armed" NK cells containing high levels of cytotoxic proteins such as perforin. The adaptive NK subset was markedly expanded in a subset of severe patients. These findings lay the groundwork for future studies examining the mechanisms of NK cell activation in COVID-19 and their potential roles in host protection and immunopathology. Understanding innate immune responses in coronavirus disease 2019 (COVID-19) is important to decipher mechanisms of host responses and interpret disease pathogenesis. Natural killer (NK) cells are innate effector lymphocytes that respond to acute viral infections but might also contribute to immunopathology. Using 28-color flow cytometry, we here reveal strong NK cell activation across distinct subsets in peripheral blood of COVID-19 patients. This pattern was mirrored in single-cell RNA sequencing signatures of NK cells in bronchoalveolar lavage from COVID-19 patients. Unsupervised high-dimensional analysis of peripheral blood NK cells furthermore identified distinct NK cell immunotypes that were linked to disease severity. Hallmarks of these immunotypes were high expression of perforin, NKG2C, and Ksp37, reflecting increased presence of adaptive NK cells in circulation of patients with severe disease. Last, arming of CD56
bright NK cells was observed across COVID-19 disease states, driven by a defined protein-protein interaction network of inflammatory soluble factors. This study provides a detailed map of the NK cell activation landscape in COVID-19 disease. [ABSTRACT FROM AUTHOR]- Published
- 2020
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18. Fluid phase recognition molecules in neutrophil-dependent immune responses.
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Jaillon, Sébastien, Ponzetta, Andrea, Magrini, Elena, Barajon, Isabella, Barbagallo, Marialuisa, Garlanda, Cecilia, and Mantovani, Alberto
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NEUTROPHILS , *NATURAL immunity , *PATTERN perception , *PATHOGENIC microorganisms , *PEPTIDOGLYCANS - Abstract
The innate immune system comprises both a cellular and a humoral arm. Neutrophils are key effector cells of the immune and inflammatory responses and have emerged as a major source of humoral pattern recognition molecules (PRMs). These molecules, which include collectins, ficolins, and pentraxins, are specialised in the discrimination of self versus non-self and modified-self and share basic multifunctional properties including recognition and opsonisation of pathogens and apoptotic cells, activation and regulation of the complement cascade and tuning of inflammation. Neutrophils act as a reservoir of ready-made soluble PRMs, such as the long pentraxin PTX3, the peptidoglycan recognition protein PGRP-S, properdin and M-ficolin, which are stored in neutrophil granules and are involved in neutrophil effector functions. In addition, other soluble PRMs, such as members of the collectin family, are not expressed in neutrophils but can modulate neutrophil-dependent immune responses. Therefore, soluble PRMs are an essential part of the innate immune response and retain antibody-like effector functions. Here, we will review the expression and general function of soluble PRMs, focusing our attention on molecules involved in neutrophil effector functions. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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19. Multiple Myeloma Impairs Bone Marrow Localization of Effector Natural Killer Cells by Altering the Chemokine Microenvironment.
- Author
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Ponzetta, Andrea, Benigni, Giorgia, Antonangeli, Fabrizio, Sciumè, Giuseppe, Sanseviero, Emilio, Zingoni, Alessandra, Ricciardi, Maria Rosaria, Petrucci, Maria Teresa, Santoni, Angela, and Bernardini, Giovanni
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MULTIPLE myeloma , *KILLER cells , *CHEMOKINES , *ANTINEOPLASTIC agents , *IMMUNE response ,BONE marrow cancer - Abstract
Natural killer (NK) cells are key innate immune effectors against multiple myeloma, their activity declining in multiple myeloma patients with disease progression. To identify the mechanisms underlying NK cell functional impairment, we characterized the distribution of functionally distinct NK cell subsets in the bone marrow of multiple myeloma-bearing mice. Herein we report that the number of KLRG1- NK cells endowed with potent effector function rapidly and selectively decreases in bone marrow during multiple myeloma growth, this correlating with decreased bone marrow NK cell degranulation in vivo. Altered NK cell subset distribution was dependent on skewed chemokine/chemokine receptor axes in the multiple myeloma microenvironment, with rapid downmodulation of the chemokine receptor CXCR3 onNKcells, increased CXCL9 and CXCL10, and decreased CXCL12 expression in bone marrow. Similar alterations in chemokine receptor/chemokine axes were observed in patients with multiple myeloma. Adoptive transfer experiments demonstrated that KLRG1- NK cell migration to the bone marrow was more efficient in healthy than multiple myeloma-bearing mice. Furthermore, bone marrow localization of transferred CXCR3-deficient NK cells with respect to wild type was enhanced in healthy and multiple myeloma-bearing mice, suggesting that CXCR3 restrains bone marrow NK cell trafficking. Our results indicate that multiple myeloma-promoted CXCR3 ligand upregulation together with CXCL12 downmodulation act as exit signals driving effector NK cells outside the bone marrow, thus weakening the antitumor immune response at the primary site of tumor growth. [ABSTRACT FROM AUTHOR]
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- 2015
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20. Multiple levels of chemokine receptor regulation in the control of mouse natural killer cell development.
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Bernardini, Giovanni, Benigni, Giorgia, Antonangeli, Fabrizio, Ponzetta, Andrea, and Santoni, Angela
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CHEMOKINE receptors ,LYMPHOCYTES ,CHEMOKINES ,KILLER cells ,CELL physiology - Abstract
Chemokines play a fundamental role in lymphocyte development, mainly attributable to the control of the correct localization in the proper microenvironments of cells undergoing maturation. Natural killer (NK) cell development occurs in the bone marrow (BM) where their localization is regulated by the balance of chemokine function in cell retention into tissues and mobilization into circulation. In addition, NK cells from several extra-medullary tissues are phenotypically and functionally different from their circulating counterpart suggesting that maturation can be completed in organs other than BM. Indeed, a role of chemokines in NK cell localization into tissues during homeostatic conditions is also documented. In this review,we summarize the current notion related to the relevance of several chemokine/chemokine receptor axes in NK cell development with a focus on the regulation of their expression and function. [ABSTRACT FROM AUTHOR]
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- 2014
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21. CX3CR1 Regulates the Maintenance of KLRG1+ NK Cells into the Bone Marrow by Promoting Their Entry into Circulation.
- Author
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Ponzetta, Andrea, Sciumè, Giuseppe, Benigni, Giorgia, Antonangeli, Fabrizio, Morrone, Stefania, Santoni, Angela, and Bernardini, Giovanni
- Subjects
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KILLER cells , *BONE cells , *BONE marrow , *IMMUNE system , *CALCIFICATION - Abstract
NK cell differentiation mainly occurs in the bone marrow (BM) where a critical role in the regulation of developing lymphocyte distribution is played by members of the chemokine receptor family. In mouse, the chemokine receptor CX3CR1 identifies a late stage of NK cell development characterized by decreased effector functions and expression of the inhibitory receptor KLRG1. The role of CX3CR1 in the regulation of differentiation and positioning of NK cell subsets in theBMis not known. In this study, we found that CX3CR1 deficiency leads to accumulation of KLRG1+ NK cells in BM during steady-state conditions. The NK cell subset that expresses the receptor in wild-type mice was expanded in several tissues of CX3CR1-deficient mice, and NK cell degranulation in response to sensitive target cell stimulation was enhanced, suggesting a regulatory role of CX3CR1 in NK cell positioning and differentiation in BM. Indeed, the observed NK cell expansion was not due to altered turnover rate, whereas it was associated with preferential accumulation in the BM parenchyma. In addition, a role of CX3CR1 in NK cell trafficking from BM and spleen was evidenced also during inflammation, as CX3CR1-deficient NK cells were more prompt to exit the BM and did not decrease in spleen in response to polyinosinic-polycytidylic acid-promoted hepatitis. Overall, our results evidenced a relevant role of CX3CR1 in the regulation of NK cell subset exit from BM during homeostasis, and suggest that defect in the CX3CR1/CX3CL1 axis alters NK cell trafficking and functional response during inflammatory conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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22. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors.
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Ponzetta, Andrea, Carriero, Roberta, Carnevale, Silvia, Barbagallo, Marialuisa, Molgora, Martina, Perucchini, Chiara, Magrini, Elena, Gianni, Francesca, Kunderfranco, Paolo, Polentarutti, Nadia, Pasqualini, Fabio, Di Marco, Sabrina, Supino, Domenico, Peano, Clelia, Cananzi, Ferdinando, Colombo, Piergiuseppe, Pilotti, Silvana, Alomar, Suliman Yousef, Bonavita, Eduardo, and Galdiero, Maria Rosaria
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T cells , *SARCOMA , *NEUTROPHILS , *IMMUNE response , *TUMORS , *RNA sequencing - Abstract
Neutrophils are a component of the tumor microenvironment and have been predominantly associated with cancer progression. Using a genetic approach complemented by adoptive transfer, we found that neutrophils are essential for resistance against primary 3-methylcholantrene-induced carcinogenesis. Neutrophils were essential for the activation of an interferon-γ-dependent pathway of immune resistance, associated with polarization of a subset of CD4− CD8− unconventional αβ T cells (UTC αβ). Bulk and single-cell RNA sequencing (scRNA-seq) analyses unveiled the innate-like features and diversity of UTC αβ associated with neutrophil-dependent anti-sarcoma immunity. In selected human tumors, including undifferentiated pleomorphic sarcoma, CSF3R expression, a neutrophil signature and neutrophil infiltration were associated with a type 1 immune response and better clinical outcome. Thus, neutrophils driving UTC αβ polarization and type 1 immunity are essential for resistance against murine sarcomas and selected human tumors. • Neutrophils mediate antitumor response by sustaining an IL-12/IFNγ-dependent pathway • Neutrophils are essential for unconventional αβ T cell (UTCαβ) type 1 polarization • Type 1 UTC αβ possess an innate-like phenotype and display antitumor potential in vivo • Neutrophil infiltration is associated with good prognosis in selected human tumors Tumor-associated neutrophils (TANs) have mainly been portrayed as tumor-promoters. Here, we describe a novel antitumor pathway in which TANs promote IL-12 production by macrophages, leading to type 1 polarization of a subset of unconventional αβ T cell (UTC αβ). Type 1 UTC αβ possess an innate-like phenotype and antitumor potential in vivo. In selected human tumors, neutrophil infiltration is associated with type 1 immunity and better clinical outcome. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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23. Natural killer cell recognition of in vivo drug-induced senescent multiple myeloma cells.
- Author
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Antonangeli, Fabrizio, Soriani, Alessandra, Ricci, Biancamaria, Ponzetta, Andrea, Benigni, Giorgia, Morrone, Stefania, Bernardini, Giovanni, and Santoni, Angela
- Subjects
KILLER cells ,IMMUNOREGULATION ,MELPHALAN ,MULTIPLE myeloma treatment ,KILLER cell receptors ,CELLULAR aging ,CANCER ,THERAPEUTICS - Abstract
Recognition of tumor cells by the immune system is a key step in cancer eradication. Melphalan is an alkylating agent routinely used in the treatment of patients with multiple myeloma (MM), but at therapeutic doses it leads to an immunosuppressive state due to lymphopenia. Here, we used a mouse model of MM to investigate the ability ofin vivotreatment with low doses of melphalan to modulate natural killer (NK) cell activity, which have been shown to play a major role in the control of MM growth. Melphalan treatment was able to enhance the surface expression of the stress-induced NKG2D ligands RAE-1 and MULT-1, and of the DNAM-1 ligand PVR (CD155) on MM cells, leading to better tumor cell recognition and killing by NK cells, as highlighted by NK cell increased degranulation triggered by melphalan-treated tumor cells. Remarkably, NK cell population was not affected by the melphalan dose used, but rather displayed activation features as indicated by CD107a and CD69 expression. Furthermore, we showed that low doses of melphalan fail to induce tumor cell apoptosis, but promote thein vivoestablishment of a senescent tumor cell population, harboring high levels of the stress-induced ligands RAE-1 and PVR. Taken together our data support the concept of using chemotherapy in order to boost antitumor innate immune responses and report the possibility to induce cellular senescence of tumor cellsin vivo. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
- Full Text
- View/download PDF
24. Role of complement in cancer-related inflammation.
- Author
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Magrini, Elena, Di Marco, Sabrina, Berthenet, Kevin, Barbagallo, Maria Luisa, Polentarutti, Nadia, Ponzetta, Andrea, Mantovani, Alberto, and Garlanda, Cecilia
- Subjects
- *
COMPLEMENT activation , *ANAPHYLATOXINS , *INFLAMMATION , *TUMOR microenvironment , *MACROPHAGES - Published
- 2017
- Full Text
- View/download PDF
25. Role of complement in cancer-related inflammation.
- Author
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Di Marco, Sabrina, Magrini, Elena, Berthenet, Kevin, Barbagallo, Maria Luisa, Polentarutti, Nadia, Ponzetta, Andrea, Mantovani, Alberto, and Garlanda, Cecilia
- Subjects
- *
INFLAMMATION , *NATURAL immunity , *ANTINEOPLASTIC agents , *CARCINOGENESIS , *COMPLEMENT activation - Published
- 2017
- Full Text
- View/download PDF
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