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Financial aid administrators administer various aspects of financial assistance programs; oversee, direct, coordinate, evaluate, and provide training for program activities and the personnel who manage office operations and supervise support staff; and ensure alignment of student and institutional needs while protecting the public interest. They have long recognized the value of professional standards in this complex field. This study uses Peterson's (2011) professionalization theory, 2010 archival data, analyses of variance, and risk estimation to examine the need for, benefits of, and level of support for certification, credentialing, and establishing basic core standards for financial aid practitioners. Among 2,756 survey respondents, 72% agreed with the need for basic core standards; 82% agreed professionalization would increase the stature of and respect for the occupation; and 79% agreed to support a process if adopted. A voluntary process, mandatory basic training, a recognized financial aid curriculum, and a phase-in period for current practitioners were the components agreed on most (ICC[subscript A] = 0.98, a[subscript 2] = 0.01). With an item reliability index of alpha = 0.91 (p = 0.001) and a margin of error of ±1.75% at the 95% confidence level, the results indicate financial aid administrators want to move forward with certification, credentialing, and setting standards.

This exercise is designed to illustrate how challenging it is to listen. Although listening is an activity that people use more than speaking, reading, or writing, it is typically not afforded the same level of instruction and focus (Adler & Rodman, 2011). In addition, humans are subject to such continual and diverse stimuli; most do not even realize that they are poor listeners and that listening is hard. Students in particular spend the majority of their time in situations where listening is the primary activity they are engaging in and is key to their success, yet talk is the skill often more privileged than listening and explicitly taught.In this activity, students will take part in a class exercise that contrasts listening for information and listening for enjoyment, yet they are not aware of it until the activity has been completed and class discussion and debriefing has taken place. They will be able to recognize their behaviors and emotions when faced with paying attention to unfamiliar material.

The transfer of repatriate international knowledge to their colleagues is essential for organisations to capitalise on the benefits international assignments can offer and to gain a competitive edge. Literature on knowledge sharing and repatriate effectiveness was reviewed for the development of the repatriate knowledge sharing environment (RKSE) measure. The first phase of the study created and pilot tested a proposed seven-dimension measure of the RKSE. Phase two confirmed the dimensions of the measure and examined the validity of the measure. The first phase alluded to an eight-dimension RKSE factor structure: source credibility, knowledge quality, source awareness, employee interaction, source recognition, workplace professionalism, supervisor support and promotion of knowledge exchange. Phase two exploratory and confirmatory factor analysis confirmed the eight-dimension RKSE scale. Furthermore, construct validity was found for the eight dimensions of RKSE scale and criterion-related validity was established. Through the use of this new instrument, organisations can improve their understanding of the organisations support towards repatriate knowledge management. [ABSTRACT FROM AUTHOR]

Abstract: Escherichia coli DNA adenine methyltransferase (Dam) and Leucine-responsive regulatory protein (Lrp) are key regulators of the pap operon, which codes for the pilus proteins necessary for uropathogenic E. coli cellular adhesion. The pap operon is regulated by a phase variation mechanism in which the methylation states of two GATC sites in the pap regulatory region and the binding position of Lrp determine whether the pilus genes are expressed. The post-replicative reassembly of Dam, Lrp, and the local regulator PapI onto a hemimethylated pap intermediate is a critical step of the phase variation switching mechanism and is not well understood. We show that Lrp, in the presence and in the absence of PapI and nonspecific DNA, specifically protects pap regulatory GATC sites from Dam methylation when allowed to compete with Dam for assembly on unmethylated and hemimethylated pap DNA. The methylation protection is dependent upon the concentration of Lrp and does not occur with non-regulatory GATC sites. Our data suggest that only at low Lrp concentrations will Dam compete effectively for binding and methylation of the proximal GATC site, leading to a phase switch resulting in the expression of pili. [Copyright &y& Elsevier]

Abstract: Transcriptional regulatory proteins typically bind specific DNA sequences with ∼103–107-fold higher affinity than non-specific DNA and this discrimination is essential for their in vivo function. Here we show that the bacterial leucine-responsive regulatory protein (Lrp) does not follow this trend and has a ∼20–400-fold binding discrimination between specific and non-specific DNA sequences. We suggest that the dual function of Lrp to regulate genes and to organize DNA utilizes this unique property. A ∼20-fold decrease in binding affinity from specific DNA is dependent upon cryptic binding sites, including the sequence GN2-3TTT and A-tracts. Removal of these sites still results in high binding affinity, only ∼70-fold weaker than that of specific sites. Similar to Lrp''s binding of specific sites in the pap and ilvIH promoters, Lrp binds cooperatively to non-specific DNA; thus, protein/protein interactions are important for both specific and non-specific DNA binding. When considering this cooperativity of Lrp binding, the binding selectivity to specific sites may increase to a maximum of ∼400-fold. Neither leucine nor the pap-specific local regulator PapI alter Lrp''s non-specific binding affinity or cooperative binding of non-specific DNA. We hypothesize that Lrp combines low sequence discrimination and relatively high intracellular protein concentrations to ensure its ability to regulate the transcription of specific genes while also functioning as a nucleoid-associated protein. Modeling of Lrp binding data and comparison to other proteins with regulatory and nucleoid-associated properties suggests similar mechanisms. [Copyright &y& Elsevier]

Escherichia coli DNA adenine methyltransferase (Dam) plays essential roles in DNA replication, mismatch repair and gene regulation. The differential methylation by Dam of the two GATC sequences in the pap promoter regulates the expression of pili genes necessary for uropathogenic E.coli cellular adhesion. Dam processively methylates GATC sites in various DNA substrates, yet the two pap GATC sites are not processively methylated. We previously proposed that the flanking sequences surrounding the two pap GATC sites contribute to the enzyme''s distributive methylation. We show here that replacement of the poorly methylated pap GATC sites with sites predicted to be processively methylated indeed results in an increase in Dam processivity. The increased processivity is due to a change in the methyltransfer kinetics and not the binding efficiency of Dam. A competition experiment in which the flanking sequences of only one pap GATC site were altered demonstrates that the GATC flanking sequences directly regulate the enzyme''s catalytic efficiency. The GATC flanking sequences in Dam-regulated promoters in E.coli and other bacteria are similar to those in the pap promoter. Gene regulation from some of these promoters involves mechanisms and proteins that are quite different from those in the pap operon. Further, GATC sequences previously identified to remain unmethylated within the E.coli genome, but whose function remains largely unassigned, are flanked by sequences predicted to be poorly methylated. We conclude that the GATC flanking sequences may be critical for expression of pap and other Dam-regulated genes by affecting the activity of Dam at such sites and, thus, its processivity. A model is proposed, illustrating how the sequences flanking the GATC sites in Dam-regulated promoters may contribute to this epigenetic mechanism of gene expression, and how flanking sequences contribute to the diverse biological roles of Dam. [Copyright &y& Elsevier]